Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F90 I4 R1 13 51.2 939505 89.5% 840856 286.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,695,705 T→G I123L (ATT→CTT)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,695,7050TG97.5% 125.7 / ‑5.1 41I123L (ATT→CTT) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (20/19);  minor base A (0/1);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCA‑CCACCTGCCAGTCAACAGACGCGTGGT‑TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGATCAATTCCACAGTTTTCGC  >  NC_000913/1695410‑1695998
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     
tGAGCCGCATCACGCAGTCCAACGCGGACATCACCCTTGGCAA‑CCACCTGCCAGGCAACAGACGCGTGGT‑TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACa                                                                                                                                                                                                                                                                                                   <  2:72943/300‑1 (MQ=255)
        aTCACGCAGTTCAACGCTGACATCACCATTGGCCA‑CCACCTGCCAGTCAACAGACGCGTGGT‑TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCAGGCGGGATAGTCTGCCAGTTCAGGTCGTTTTTCACACAACCGGTGAACTGTACACTTTCCCCGGCACGAACACACgccatg                                                                                                                                                                                                                                                                                          >  1:178105/1‑296 (MQ=255)
                   aaccGCTGACTTCACCA‑TGGCCACCCACCTGCCAGGCAACAG‑CGGGTTGTGTACCGTCTGGCGCGACATGCGTCACCACGGGGATCTCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTc                                                                                                                                                                                                                                                                               <  1:34034/298‑1 (MQ=255)
                             aTCACCATTGGCCA‑CCACCTGCCAGTCAACAGACGCGTGGT‑TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGt                                                                                                                                                                                                                                                                      >  2:316063/1‑300 (MQ=255)
                                                    agttCAACAGGCCCGGGGT‑TACGTTCTTGGTGGGCAGGCTTCACCCCGGTAGTATCGTCCACCCTGGTGTTCGGCGTGGTGTAGAGGATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGAGAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCa                                                                                                                                                                                                                                               <  1:315974/298‑1 (MQ=255)
                                                      gTCAACAGACGCGTGGT‑TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCATGATGCTCCATCACt                                                                                                                                                                                                                                             >  2:214972/1‑301 (MQ=255)
                                                       tCAACAGACGCGTGGT‑TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATAAGGCGTGACATCGGCTTCAAATGGCGGATAGCAGGCCTGATg                                                                                                                                                                                                                                                       >  2:286613/1‑290 (MQ=255)
                                                                      t‑tACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTTATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCATGAGCCCCCCTCCCTTCCTTATTTTTGAccc                                                                                                                                                                                                                             >  1:417542/1‑301 (MQ=255)
                                                                      t‑tACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCCGATGCCCCATCCCTTCCTGATTATTTAccc                                                                                                                                                                                                                             >  1:376846/1‑301 (MQ=255)
                                                                      ggtACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTTATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGAcc                                                                                                                                                                                                                              <  2:230666/299‑1 (MQ=255)
                                                                                 gcgcGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAACTGGCGTATAGCCGCCCTGATGCTCCCTCCCTTCCTGATTTTTCAACCACCCTTTGcc                                                                                                                                                                                                                   >  1:397358/1‑301 (MQ=255)
                                                                                   gcgACATGCGTCACCAAGGTGATATCGTCCACCCAGGTGTTCGACGTGTTGTAGAGCATTACGCAGCGATGAATCCCGGCATAATTAAATAAAACAGGGAAGTATGACTGCTTGTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGAGAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTGTTCCCGGCAAGAACATACGGCGTGACATCGTCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGt                                                                                                                                                                                                                 <  2:27298/301‑1 (MQ=255)
                                                                                          gCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGt                                                                                                                                                                                                          <  2:417650/301‑1 (MQ=255)
                                                                                          gCGTCACCACGGTGATATCGTCCACCAAGGGGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGt                                                                                                                                                                                                          <  2:376943/301‑1 (MQ=255)
                                                                                                          atCGTCCACCCAGGTGTTCGTGGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACgc                                                                                                                                                                                          <  2:178157/301‑1 (MQ=255)
                                                                                                          atCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTc                                                                                                                                                                                                                                                    >  2:382847/1‑243 (MQ=255)
                                                                                                                   ccATGTGCTCGGCGTGGTGTCGCGCATGACTCGTCGACTCAGCCCGGCATAGTTAAACAACTCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCCCCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATa                                                                                                                                                                                 <  2:62136/301‑1 (MQ=255)
                                                                                                                    cAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACCTACGGCGTGACATCGGCTCAAAATGGCGTATAACCGCCCTGCTGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCGTCGAACCgccgcaagcacc                                                                                                                                                                                >  1:458960/1‑295 (MQ=255)
                                                                                                                                   gggtAGCGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAAc                                                                                                                                                                 <  2:397459/299‑1 (MQ=255)
                                                                                                                                                      gATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGTCAAGAACATACGGCGTGACATCGGCTTCAAATGGCTTATAGCCGCCCCGATGCTCCATCACCTCCTGCTTATTGACCCACACCTTGCCGTAATGAGTGACCGCATCCAAACGCAGCACGATACGCTGGCCCGCCCAACCTTCCCGTCTAAAGACTTc                                                                                                                                              >  1:18053/1‑301 (MQ=255)
                                                                                                                                                           aTCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCAAACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCCAATGGCTTATAGCCGCCCTGATGCCCCATCAATTCCTGCTTATTGACCCACACTTTTCCGTAATGCGTGACCGCATCGAAACGCAGCCCGATACGCCGGCCCGCCCCACCTTTCGGTATAAAAACTCcggccc                                                                                                                                         >  1:1708/1‑297 (MQ=255)
                                                                                                                                                             cccGGCATAGTTAAAGAAATCTTGGAAGTACGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATATTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGccc                                                                                                                                                                    <  2:370557/272‑1 (MQ=255)
                                                                                                                                                                   atggTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTAGCCGTTTTCGGCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACgcag                                                                                                                                                                                        <  2:22783/243‑1 (MQ=255)
                                                                                                                                                                                 cATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTTCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCCCTTATCCCAAAAGTTGCCCGCAt                                                                                                                    >  1:434482/1‑300 (MQ=255)
                                                                                                                                                                                  aTGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATa                                                                                                                   >  2:462692/1‑300 (MQ=255)
                                                                                                                                                                                             gcggCTTTTTCTTGCCGTTTTCGTCGGTGATCGCCATTCCCGGCGGGATAGTCGGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTACCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATc                                                                                                       <  2:1708/298‑1 (MQ=255)
                                                                                                                                                                                                gCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTg                                                                                                     <  2:18056/300‑1 (MQ=255)
                                                                                                                                                                                                gCTTTGTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCt                                                                                                      <  2:459084/299‑1 (MQ=255)
                                                                                                                                                                                                                atcgtccagtatcccctgtCCCGGGGAGACAGTCTGCCATGTCCGTTCTTTGTTCCCACAAACGGGGATACGTACACTTTACCCGTCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATc                                                                                     <  1:280755/282‑1 (MQ=255)
                                                                                                                                                                                                                    tcgGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATCCGCTCGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTa                                                                                 >  2:156687/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTAATCACCCTTCCCGGCGGGCAAGTCTTCCAGTTCAGTTCGTTTTTCACACAAACGGTGATACGTACACTGTTCCCGGCAAGAACATACGGCGTGACGTCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAAc                                                                             <  1:462567/301‑1 (MQ=255)
                                                                                                                                                                                                                        tAATCACCGTTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACCCAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAAc                                                                             <  1:214909/300‑1 (MQ=255)
                                                                                                                                                                                                                              ccATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCATGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGACACCTTCGCCTGCCCAACCTTTCGGTACAAAGACTTCGCCCTGATACCATACCTTGCCCCCATCATTACCAACATCTGAACCTGCTAACTGAACGTTAAAACTGTCTgg                                                                      >  1:38951/1‑301 (MQ=255)
                                                                                                                                                                                                                                               gTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTAAGAATATCAGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCACggg                                                     >  2:32743/1‑300 (MQ=255)
                                                                                                                                                                                                                                                         tccgttcGTTGTTCGCACAAACGGTGAAACGTCAACTTTTCCCGGCAATGACAGACGGCGTGACATCGGCTTCAAATGGGGTATAGCCGCCCTGATTCTCCATACCTTCCTGATGATTGCCCCTCACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTa                                            <  1:122381/297‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tcaaaaaaaCGGTGATACGTACACTTTTGCCGGCAAAAACAAACGGCGTTACATCGGCTTCACATGGCGTATAGCCGCCCTGATGCACCCTCACCTCCCGCTTATTGACCCACACTTTGCCGGAATGCGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTccc                                <  1:156645/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTCTAAAg                                                                                                                                                   >  1:35772/1‑185 (MQ=255)
                                                                                                                                                                                                                                                                     tCACACAAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAg                                                                                                                                                   <  2:35782/185‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cacacaAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGCCGTTGCCCGCATAATTACGAATATCTTCCTCGGTGAACTGATCGTTAAAACTGCCTGGCACCGCAATTTCCCGGCTTTCTTTTAACGCGCTTTCccac                              >  2:298736/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                        cacaAACGGTGATACGTACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCCCTGATACCAGACGTTGCCCGCATATGTACGAATATCTGCATAGGCGAACTGATCGTTAAAACTGCCTGGCACACCAATTTCCCGCCTTTCTTGTAACCCGCTTTCccacc                             >  1:461093/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  ccGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGACCGGCTTTCTTGTAACGCGCTTTCCCACACACGCTGATCAATTCCACAGTTTTcgc  >  2:158809/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     
TGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCA‑CCACCTGCCAGTCAACAGACGCGTGGT‑TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGATCAATTCCACAGTTTTCGC  >  NC_000913/1695410‑1695998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A