Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F90 I4 R1 13 51.2 939505 89.5% 840856 286.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,187,507 A→C N720H (AAC→CAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,5070AC98.0% 150.8 / NA 50N720H (AAC→CAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (26/22);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACC  >  NC_000913/4187215‑4187797
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                   
ccAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAAATGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCGGGTTTCCTTCCACAGCATc                                                                                                                                                                                                                                                                                             >  2:436298/1‑301 (MQ=255)
  aGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCt                                                                                                                                                                                                                                                                                            >  2:459195/1‑300 (MQ=255)
      actgtagcccGGGTTCGCTCATGAAGGGGGTTCGGGGGCATCTGTTGGTATCGATGA‑CATGGGCATCCCGGAGAAGAAAAACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATAGCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACa                                                                                                                                                                                                                                                                                         <  1:350749/291‑1 (MQ=255)
                             cAGCGCGTTCTGGTGCATCTGTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGCCAGGAAGAGAAGAAGGTTTCCTTCCACAACATCAACATGATGGCCGCCGTGGGTGCGCGTgg                                                                                                                                                                                                                                                                >  1:153985/1‑301 (MQ=255)
                                        ggTGCATCTGTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAACTCATCTCCGAGGCAGAAGCAGAAGTTGGTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGcac                                                                                                                                                                                                                                                     <  1:178779/301‑1 (MQ=255)
                                                tgatGGTACCGTTGACCATGGCCA‑CCCGGAAAAAAAACACGAAATCAGCCCCGAGGAAGAAGCAGAATTTTCGGAAATTCGGGGGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGGTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTc                                                                                                                                                                                                                                               <  1:336043/296‑1 (MQ=255)
                                                tGTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGGGAAGCAGGTTTCCTTCCACAGCACCTACAAGATGGCCGACTCAGGTGCGCGGGGTTCTGGGGCACGGAtt                                                                                                                                                                                                                                                >  1:97645/1‑298 (MQ=255)
                                                tGTTGGTATCGATGA‑CATGGTCAGCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGAtt                                                                                                                                                                                                                                                <  2:97669/298‑1 (MQ=255)
                                                 gTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTAAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACGCCGGTGCGCGTGGGTCTGCGGCACAGATTcagg                                                                                                                                                                                                                                            >  2:336136/1‑298 (MQ=255)
                                                     ggtaatgatgcactGGTCATACTGAAGAAGAAACACGAAATCGTCTCCGAGGCGGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCAAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGc                                                                                                                                                                                                                                        <  1:69734/288‑1 (MQ=255)
                                                     gaaTCGATGA‑AATGGTCAACCCGGAGAAGAAACACGAAATCATTTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGGAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCt                                                                                                                                                                                                                                       <  1:459071/299‑1 (MQ=255)
                                                             gA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTAACGGTAAGGAAGAAAAGCAGGTTTCCTTACACAGCATCTAAATGATGGCCGACTCCGGTGCGAGTGGTTCTGCGGCACAGATTATG‑TAGCTTGCTGGtt                                                                                                                                                                                                                               >  2:367769/1‑300 (MQ=255)
                                                                              agaagaAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACCCCGGTGCGATGGGTTCGGCGGCCCAGAGTCGT‑CAGCTTGCTGGTAGGCGTGGTCTGAgggg                                                                                                                                                                                                               >  1:367906/1‑300 (MQ=255)
                                                                                 agaaACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGTTCAGGAAGAGAAGCAGGTTTACTGCCACAGCATATACATGATGGCCGACTCTGGTTCGCGAGGTTCTGCGGC‑CCGGCTCGTGCAGCTTGCTGGTAGGCGGGGTTTGATGGCGa                                                                                                                                                                                                             >  1:144574/1‑300 (MQ=255)
                                                                                     acacGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACCCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGGGGGGTCTGTTGGCGAAGCCg                                                                                                                                                                                                        >  1:429185/1‑301 (MQ=255)
                                                                                                tctcCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTTTCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCAAAGCATCTACATGATGGCAGACTCCGGTGACCGTGTTTTGGAGGAACAGGTTCGT‑CAGCTTGATGGTATCGGTGGTCTAATGGCGAATACGGCTGGCTCcat                                                                                                                                                                                             >  2:133688/1‑301 (MQ=255)
                                                                                                   ccGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCCACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcctcat                                                                                                                                                                                          >  2:29426/1‑301 (MQ=255)
                                                                                                                   agTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGTTGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCTT‑CAGCTTGCTGGTATGCGTGGTCTGTTGGCGATGCCGGATGGCTCCATCATCGAACCGCCACTcccc                                                                                                                                                                          >  2:47449/1‑301 (MQ=255)
                                                                                                                      tGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAATTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTTATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAAAAGCCTCGAAATGATGGCCGATATCGGTACGCGTGCTTCCGAGGCACAGATTAGT‑CAGCTAGCTGGTATGCGTGGTGTGGTGGGGAACGCGGTAGGCCCCTTCACGGAACCGGAAATCACtgcg                                                                                                                                                                       >  2:108934/1‑301 (MQ=255)
                                                                                                                          aaaaaTCAGGAGCAGTTCCAGTCTGGTCTGGAAATTGCGGGCGAACGCTACAAAAAAGTTATCGATATCTGGGAGGCGGCGAACGATCGTGGATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACt                                                                                                                                                                   <  1:367672/300‑1 (MQ=255)
                                                                                                                              gtCAGGAGCAGATCGAGTCTGTGCGGTTACAGGAGGACGAACTATACAAAAAAGGTAAGGATTACTCGGGTGTGGCGAACGATCGGTGATCCAAAGCGCTGATGGATAACCTGCAAACTGCAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCg                                                                                                                                                               <  1:436182/300‑1 (MQ=255)
                                                                                                                                                      gggtAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTa                                                                                                                                       <  2:154027/300‑1 (MQ=255)
                                                                                                                                                              gCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGGTGCGGCGAACGATCGTTTATCCAAAGCGATGATGGATAACCAGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGAGGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAtct                                                                                                                               <  2:144610/301‑1 (MQ=255)
                                                                                                                                                                         cTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTAAGCGTGGTCTTATGGCGAAGCCGGATGGCCCCATCAACGAAAAGCCAACCACCGCGAAATCCCGTGCAGGTCTGAACGTACCCCAGTCCTTCATCTCCCCCCCCggc                                                                                                                    >  1:425074/1‑300 (MQ=255)
                                                                                                                                                                               caaAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAgg                                                                                                                                                         <  2:395011/258‑1 (MQ=255)
                                                                                                                                                                                  aGTAATCGATAGCGGTGTGGCGGCGAACGAGCGTGTGTCAGAAGCGATGATGGATAACCTGCAAACAGATGCCGTGATTAACCGTGACGGTCAGGAAGAGAAACAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTaaa                                                                                                           <  2:199383/301‑1 (MQ=255)
                                                                                                                                                                                     tATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                                         <  2:429296/300‑1 (MQ=255)
                                                                                                                                                                                          atatCTGGGGTGGGGCGCGCGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                   <  1:166047/301‑1 (MQ=255)
                                                                                                                                                                                          aaacggcggCTGCGGCGAAAGAGCGTGTAGTCAAGACGATGATGGAGAACCTGCAAACTGAAACCGGGATTAACCGTGACGGTCAGTAAGATAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTAGGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                   <  1:47437/294‑1 (MQ=255)
                                                                                                                                                                                           aaacagggacgggacgAACGTTTGTGTAGCAAAAGCGATTATGGAGTACCTGAAAACTGAAAGCGTTATTTACCGTGACGGGCCGGAAGACAAGCAGGTTTCCTCCGACAGCGTCTTCATGCTGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGCCTCCATCATCGAAACGCCAATCACCGCGATCTTCCGTGAAGGTCTGAACGTACTCCAGTACTTTATTTCCACCCACGGTGCTCGTAAAGGTCTGGCg                                                                                                  <  2:81758/287‑1 (MQ=255)
                                                                                                                                                                                            atCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCgg                                                                                                 >  2:454207/1‑301 (MQ=255)
                                                                                                                                                                                                             cgagcgttgacccAAAGCGATGATGGATAACCTGCAAACTGAGACCGTGATGAACCGTTACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACGGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACt                                                                                <  1:454086/290‑1 (MQ=255)
                                                                                                                                                                                                             cGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATACCCACCCACGGTGCTCGTACAGGTCTGGCGGATACCGCACTGAAACCt                                                                                >  1:63629/1‑301 (MQ=255)
                                                                                                                                                                                                                    gtttCCAAAGCGATTATGGACAACCTGAGAAAGTAAACCTGTATGACCCGTGACGGTCAGGAATAACAGCAGGTTTCCTTCCAAAGCATCTACATGTTGGCCGACATCGGTAGGCGTGGTTCTTAGCCAACGATTCTA‑CCGCTTGCTGGGATGCGTCGTCTGAGGGAGAAGCCGGATGGCTCCATCATCGAAACTCCCATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAAc                                                                          <  1:133655/297‑1 (MQ=255)
                                                                                                                                                                                                                     tATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTTATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCCCCATCATCGAAACGCCAATCACCGCGCAATTCCGGGAGGGTCTGAACGTACCCCAGCACTTCATCTCCACCCCCGCTGCTCGTCACGGCCTGGCGGATCCCGCACTCACAACAGCGAAccc                                                                        >  1:25988/1‑299 (MQ=255)
                                                                                                                                                                                                                        caaagaccattgtgGATAGCCTGCGATCTGATCCCGTGATTATCCTGGACGGGCAGGAGGACACGCAGCTTTTCTTGCACCGCAGCTAGATGAGTGCCGACGCCTGTGCGCGTGGTTCTGCGGCACAGATCCGT‑CAGCTTGCTGGGATGCGTGGTCTTATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTCCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCgg                                                                     <  1:49586/289‑1 (MQ=255)
                                                                                                                                                                                                                              cGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCt                                                               <  2:368003/301‑1 (MQ=255)
                                                                                                                                                                                                                                              gCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCCCAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGCACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCAACCCACGGTGCTCGTAACgg                                                                                                         >  2:353223/1‑243 (MQ=255)
                                                                                                                                                                                                                                               cAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGCCTGGTTg                                              >  2:413634/1‑301 (MQ=255)
                                                                                                                                                                                                                                               aaaaCGGAAACCGTGATGAACCGGGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCAACGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTg                                              <  2:425185/300‑1 (MQ=255)
                                                                                                                                                                                                                                                   cTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGGAGCAGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCCCTGAAAACTGCACACTCCGGTTCCCTgctgctccggccggttgccgg                                          >  1:308466/1‑281 (MQ=255)
                                                                                                                                                                                                                                                    tGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTATGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACGTCATCTCCACCCACGGTTCTCGTAAAGGTCTGGCGGATACGGCACTGCAAACTGCGAACGCCGGTTACCTGCCTCGTAGTATGGtagtagtg                                         >  2:136215/1‑294 (MQ=255)
                                                                                                                                                                                                                                                     gacaCCGTGAGTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACAGGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTgg                                        <  2:345122/298‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTATAGGTCTGGCGGCTACCGCCTTGAAAACTGAGCACCCCggagagcggtctcgcgtcctgtcaggagagcg                                       >  1:257532/1‑271 (MQ=255)
                                                                                                                                                                                                                                                      aaaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCgg                                                                     <  2:257608/271‑1 (MQ=255)
                                                                                                                                                                                                                                                             gATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTTACTCGTCGCCTGGTTGACGTGGCACAGGAc                                >  1:178767/1‑301 (MQ=255)
                                                                                                                                                                                                                                                             gATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGGCTGGCGGATACCGCCCTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcccgggcc                                >  1:43129/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                   ccGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAGACCGCACTGAAAACTGCGCACTCCGGTTACCTGACTCGTCGTCTGGCTGACGTGGCGCAGGACCtggtg                          >  1:371342/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                gAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGGGCAGGTCTGAACGTACTCCAGTACTTCATCCCCACCCACGGTGCCCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGCACTCCGGTTACCTGAACCGTCCTCTGGTTGACGTTGCGACGGCCGTGGGGGTTTCCGAAGACg             >  1:368/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                           ggTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTAcc  >  2:312398/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                   
CCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGA‑CATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGT‑CAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACC  >  NC_000913/4187215‑4187797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A