Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F9 I0 R1 113 83.2 1840812 91.6% 1686183 224.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,748,910 A→G 12.3% E112G (GAG→GGG)  sgbH → 3‑keto‑L‑gulonate 6‑phosphate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,748,9100AG12.3% 207.8 / 2.7 94E112G (GAG→GGG) sgbH3‑keto‑L‑gulonate 6‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (41/37);  new base G (2/12);  total (44/50)
Fisher's exact test for biased strand distribution p-value = 9.14e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCGCTGTTTAAAGATATCCGCGTGAAAGCGTTTATTGCCGG  >  NC_000913/3748674‑3749147
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
ggCGGGCCCCATTCTCTGTTTAAACGAAGGGCTTGGGGCGGTGAAAGCCTGGCGCGAACAGTGCCCGGACAAAATCAACGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATagag                                                                                                                                                                                                                                                  <  2:742931/237‑1 (MQ=255)
 gCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGc                                                                                                                                                                                                                                                 <  1:500697/238‑1 (MQ=255)
 gCGGGCACCATTCTCGGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGc                                                                                                                                                                                                                                                 <  1:769956/238‑1 (MQ=255)
   gggccgcATTCTGTGTTTAAACGAAAGGCTTGGCGGTGTGAAAGCCTTGCGCGAACCGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGGCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTg                                                                                                                                                                                                                                               <  1:342762/232‑1 (MQ=255)
                  tttAAACGAAGGGTTGGGGGGGGTTAAAGCGTTGCGAGAACAGTGCCCGGACAAAATCATCGTGGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCGCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGAGGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAActggacgc                                                                                                                                                                                                                               <  2:310418/239‑1 (MQ=255)
                   ttAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCCCA‑ACGC‑TGCGGGGGTGAAATTCAGATAGGGCGGTTCGGTAActggac                                                                                                                                                                                                                                 >  1:386623/1‑236 (MQ=255)
                        cGAAGGGCTTGGCGCGGTGAAAGCCATGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGGCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGTCCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAActggacgctggacg                                                                                                                                                                                                                         <  1:130737/239‑1 (MQ=255)
                            gggCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGccgacg                                                                                                                                                                                                                      >  1:319311/1‑238 (MQ=255)
                                 tGGCGGGGTTAAAGCCTGGCGCCAACGGTGCCCGGAAAAAATCCTGGTTGCTGGCTGGAAGGGCGCCGACGCTGGGGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCc‑gcg                                                                                                                                                                                                               <  2:679950/239‑1 (MQ=255)
                                   ggcgggTGAAATCCTTGGGCGAACAGGGCCCGGACACAATCATCGTGGCGGAGTGGAAGGGCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGAc                                                                                                                                                                                                             <  2:319311/235‑1 (MQ=255)
                                    ggggtggcaaactttGCGCGGAGCGTGCCCGGACAAATGCCTCGTTGCTGACTGGAAGGTCGCCGTCGCTGGGGAAACGCCCGCGCAACATGCGTTTGGCGCAGGCGCTAACGGGAAGCCCATCATCTGCGCCGGGCCGCTCGGGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑CGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTATCTGGCCGCTGGACGACGCCC‑GCGAc                                                                                                                                                                                                             <  2:194402/225‑1 (MQ=255)
                                        gTGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGAGCAACAGGCGTTTGGGGCCGGCGCTCACTGGATGACAATCATCTGCGCCGCGCCGCTCGCGCCGGTAGACAAAGGACACGCAAAGGCACA‑GCGC‑TACGGGGGTGACATTCAAATCGAGCAGGCCGGTAactgg‑cactggacgtatgaccgcatcgggcc                                                                                                                                                                                                        >  2:67275/1‑222 (MQ=255)
                                         tGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAACCGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCa                                                                                                                                                                                                        <  1:633310/238‑1 (MQ=255)
                                                  tGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGTTGACGCTGTTCGGTAACTGACCGCCGGACGACCCCC‑GCGACGGGAATCGGATTggg                                                                                                                                                                                              >  1:308168/1‑238 (MQ=255)
                                                  tGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGGGAAATTCAGATAGAGCGGTTCGGAACATGAACGCTGGCGGACCCCC‑GGGACGGGCACCGAATTGGc                                                                                                                                                                                              >  2:150937/1‑239 (MQ=255)
                                                  tGCGCGAACAGTGCCCGGACAAAATCATCGATGCTGACTGGAAGGTCGCCGACGCTTGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGTCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGCCGGTAGCAAAAGGCAACGCAATGG‑ACACACGC‑TGCGGGAGGGAAAGTCCAATAGAAGTGGTCGGTGAGCGGACCCTGGCCGAAGCCC‑GTGaagggccaggcattggg                                                                                                                                                                                              >  2:635882/1‑223 (MQ=255)
                                                   gcgcGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGCCGGTAGAAAAAGGCCAAGCAATGGCACA‑ACGC‑TCCGGGGGGGGAATTCAGATAGAGCGGTCAGGGAACTGGACGCTGGGCAGCGCCc‑ggaaccggctccgagttgggg                                                                                                                                                                                             >  2:722864/1‑219 (MQ=255)
                                                   gcgcGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGAGGACGCCG‑GCGACTGGCATCGTATTGGCg                                                                                                                                                                                             >  1:297764/1‑239 (MQ=255)
                                                   gcgcGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGAAACTGGACGCTGGCCGACGCCC‑GCGACCGGGATCGCATTgggg                                                                                                                                                                                             >  1:767322/1‑237 (MQ=255)
                                                   gcgcGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGGGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGCCGACGCCC‑GCGACTGGATTGGTATTGGCg                                                                                                                                                                                             >  1:570164/1‑239 (MQ=255)
                                                   gcgcGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACCC‑TGCGGGGGGGAAATTCAGCTAGAGCTGTTCGGTAACGGGACGCTGGACGACGCCC‑GCGACGGGCAACGCATGGGCg                                                                                                                                                                                             >  2:775585/1‑239 (MQ=255)
                                                   gcgcAAACAGTGCCCGGACAAAATCATCGTTGCGGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCg                                                                                                                                                                                             <  1:541260/239‑1 (MQ=255)
                                                         aCAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGCATTGGCGTGCGGc                                                                                                                                                                                       >  1:25793/1‑239 (MQ=255)
                                                         aCAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGACGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCAACGCAATGGCACA‑ACGC‑TGCGGGGGGGGAATTCAGATACAGCTGTTCGGGAACGGACCGCTGGCGGACGCAC‑GGGACGGGCATCGATTTgggggggggc                                                                                                                                                                                       >  2:346615/1‑231 (MQ=255)
                                                             tGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACCC‑TCCGGGGGGGAAATTCGATTAGAGCTGTTCGGTAACTGGACGCTGGGCGACGCCC‑CCGACGGGCATCGTATGGGCGTGGGGCggg                                                                                                                                                                                    >  2:873525/1‑238 (MQ=255)
                                                             ggCCCGGACAAAATCATCGTTGCTGACGGGAAGGTCGCCGACCCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCgg                                                                                                                                                                                        <  1:382154/233‑1 (MQ=255)
                                                              gCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAACCGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAgg                                                                                                                                                                                    <  1:791586/237‑1 (MQ=255)
                                                              gCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGGCGCCC‑GCGACTGGCATCGTATTGGCGAGCGGCAGGcc                                                                                                                                                                                  >  1:281000/1‑239 (MQ=255)
                                                              gCCCGGAAAAAATCATCGTTGCCGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCa                                                                                                                                                                                      <  1:426647/235‑1 (MQ=255)
                                                               cccGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCACCTGGCATCGTATTGGCGTGCGGCAGGc                                                                                                                                                                                   >  1:834730/1‑237 (MQ=255)
                                                                   gACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCCCA‑ACGC‑TGCGGGGGTGAAATTCAGATAGGGCTGTTCGGAAACTGAACGCGGGACGACGCCC‑GCGACTGGCAACGTATGGGCGGGCGGGAGGCCAttaa                                                                                                                                                                             >  2:426647/1‑237 (MQ=255)
                                                                   gACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCAC‑‑ACGCATGCGGGGGGGCAATTCAGATAAAACAGTTCGGAAACGGGACGCTGACCGACGCCC‑GCGACGGGCATCGGATTGGGTGGCGGCAGGCAAttaa                                                                                                                                                                             >  2:122619/1‑237 (MQ=255)
                                                                       aaaTCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCCCA‑ACGC‑TGCGGGGGGGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACGGGCATCGGATTGGGGTGGGGCAGGCACTTTaagg                                                                                                                                                                          >  2:382154/1‑235 (MQ=255)
                                                                            ctcGTTGCTGACTGGAAGGTCGCCGACGCTGGGTAAACGGCCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCgg                                                                                                                                                                    <  1:16492/238‑1 (MQ=255)
                                                                              cGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCCAATTGGGGGGCGGAAGGCCATTTataacgcggggg                                                                                                                                                                  >  2:614020/1‑229 (MQ=255)
                                                                              cGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCGGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGACATCCAGATAGAGCTGTTGGGTAACTGCACGCTGGCCGAAGCCCAGCGA‑GGGCACGGTATTGGCGGCCGGCAGGACATTGaccataccgggg                                                                                                                                                                  >  2:817308/1‑232 (MQ=255)
                                                                                      aCTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATAATCGCGGCCGTgtggc                                                                                                                                                           >  1:804997/1‑234 (MQ=255)
                                                                                           aaGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGAATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCCCAgg                                                                                                                                                      >  1:68171/1‑238 (MQ=255)
                                                                                            aGGTCGCCGACGCTGGTAAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGc                                                                                                                                                     <  1:899701/238‑1 (MQ=255)
                                                                                            aGGTCCCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGc                                                                                                                                                     <  1:150937/238‑1 (MQ=255)
                                                                                               tCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCa                                                                                                                                                   <  1:565780/237‑1 (MQ=255)
                                                                                                cGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCCTCGTATTGGCGTGCGGCAGGCCATTTATCATCCCGGCCGCGTTGCCCAGGcgcgcg                                                                                                                                                >  1:752831/1‑234 (MQ=255)
                                                                                                cGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCCTCGGATTGGCGTGGGGCAGGCCATTTATCAACCCGGTCGGTATGCACAGGCCAggg                                                                                                                                                >  2:570552/1‑237 (MQ=255)
                                                                                                cGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGGAACTGGCCGCTGGACGACGCCC‑GCGCCTGGCCTCGTATTGGGGTGCGGCAGGCCCTTTTTCATACCGGGCGGGATGCACAGGCCAggg                                                                                                                                                >  2:565780/1‑237 (MQ=255)
                                                                                                   cGACGCGGGTGAAACCCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGc                                                                                                                                             <  1:190872/239‑1 (MQ=255)
                                                                                                                 cGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTGGAAAAAGGCCACGCACTGGCACA‑ACGC‑TGCGGTGGTGCAATTCAGCTAGAGCGGTTCGGGCACTGGACGCTGGACGACGCCG‑GCGACGGGCATCGTATTCGGCTGTGGCCGGGCATTTATCAACGCGGTAGGGCACCGCAGGGCAGAGGCCAACAGGGGGGGGaa                                                                                                                               >  2:910185/1‑239 (MQ=255)
                                                                                                                  gcccgcgCAACGGGCGTTTGGAGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAg                                                                                                                              <  1:516008/236‑1 (MQ=255)
                                                                                                                     cgcgCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACACAGGGCCGAAGACg                                                                                                                           >  1:258376/1‑239 (MQ=255)
                                                                                                                     cgcgCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGGGATGCACAGGCCAGCGGCGAACAGGGGGGCGAAccc                                                                                                                            >  1:59129/1‑238 (MQ=255)
                                                                                                                                        gcgggCGTTAAATGGAGGGCCCTCCCCGGCGCGGGGCCGCGCGGGGAGGTAAAAAAAGGCCACGCAAGGGCACA‑ACGG‑TGCGGGGGTGAAATTCAGATAGAGCGTTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAgg                                                                                                        <  2:804997/235‑1 (MQ=255)
                                                                                                                                         caggcttaatcTGGCTGACCATTCTCCGCGCCCGGCCGCTCGCGAGGGTAAAAAAAGGCCCCGCAAGTGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGGGCTGTTCGGTAACTGGCCGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGgcg                                                                                                      <  2:572950/229‑1 (MQ=255)
                                                                                                                                         cAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATGCCGGTCGCGCTCCACAGCCAAGCGGGCAAACATGGGGAGGAGCCGATCTGGCACG‑CATGAggggg                                                                                                      >  2:407289/1‑237 (MQ=255)
                                                                                                                                              gcTAAATGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCttt                                                                                                  <  1:614020/238‑1 (MQ=255)
                                                                                                                                                    tGGATGACCATCATTTGGGCCGCGCCGGGCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTGCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGatat                                                                                           <  1:122619/239‑1 (MQ=255)
                                                                                                                                                      gATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAACCGATCTGGCACG‑CCTGAAGGCGCTTTCAGATATCg                                                                                         >  1:516249/1‑239 (MQ=255)
                                                                                                                                                              tcgtcTGCGCCCCGCCGCTCGGGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAg                                                                                 <  1:558513/236‑1 (MQ=255)
                                                                                                                                                              tcaacTGGGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAAGGGCACA‑ACGC‑TGCGGGGGGGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAg                                                                                 <  1:208526/239‑1 (MQ=255)
                                                                                                                                                                 tttggcccgcgccgcTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGGTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCtt                                                                              <  1:249856/233‑1 (MQ=255)
                                                                                                                                                                     cgccgcgccgCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGAATTGGCGGGCGGCAGGCCATTTATCATCTCGGTCGTGATGCCCAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGGACG‑CATGGAGGCGCGTTCGGATAACGGCCTTGGGCTTTCCa                                                                          >  2:889736/1‑239 (MQ=255)
                                                                                                                                                                             ggggcgggACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTgg                                                                   <  1:737914/231‑1 (MQ=255)
                                                                                                                                                                             cgtccgcgGCGGTAGAAAAAGGCCCCGCAATGGCACA‑ACGC‑TGCGGGGGTTAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGGCGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTgg                                                                   <  1:335613/234‑1 (MQ=255)
                                                                                                                                                                             cggtggggcggggaGAAAAAGCCCTCGCAAGGGCCCA‑ACCG‑TGGGGGGGGGAAATTCGGTAAGTGCGTTTTGGGACCTGGTCGCGGGACGCGCCCC‑GCGACTGGCCTCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGc                                                                  <  2:480662/226‑1 (MQ=255)
                                                                                                                                                                             ccccgcccgcggtcaaaaaaaGCCACGCAGTGGCACA‑ACCG‑GGCGGGGGTGAAAGTCAGGTAGGGCTGTTGGGTAACGGGCCCTTGGACGAGCCCC‑GCGACTGGCATCGGATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGc                                                                  <  2:767322/224‑1 (MQ=255)
                                                                                                                                                                               cTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGGAACAGGGGGGCGCAGCCGATCTGGCACG‑CATGAAGGCGCGTTCAGATAACGGCCTTGAGCTTTCCATTACTAGCgg                                                                >  2:126683/1‑239 (MQ=255)
                                                                                                                                                                                tCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCgg‑g                                                              <  1:775585/239‑1 (MQ=255)
                                                                                                                                                                                  gcggtggTAAAAAAAGGCCCCGCAATGGCACA‑ACGC‑GGGGGGGGTGAAATTCAGATAGGGCTGTTCGGTAACTGGACGCTGGACGCCGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GAt                                                            <  2:516249/234‑1 (MQ=255)
                                                                                                                                                                                  gcggcgGTAGAAAAAGGCCCCGCAAGGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GAt                                                            <  1:873525/239‑1 (MQ=255)
                                                                                                                                                                                   cgAGGGTAGAAAAAGGCCCCGCAATGGCACA‑ACGC‑TGGGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACGGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GAt                                                            <  2:386623/238‑1 (MQ=255)
                                                                                                                                                                                           gAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTgg                                                                   <  1:330149/224‑1 (MQ=255)
                                                                                                                                                                                                  gCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGGGGGGCGAACCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCgg‑gggaaccaccccgcacccg                                            >  2:360769/1‑221 (MQ=255)
                                                                                                                                                                                                   ccACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAACTATCGGCCTTGAGCTTTCCATTACTGGCGG‑GGTTACCCctcct‑gtgctg                                           >  1:274528/1‑233 (MQ=255)
                                                                                                                                                                                                     cggccaggggcca‑ACGC‑GGGGGGGGGGAAATTCAGGTGGAGCTGTTGGGTAACGGGCGGCTGGACGACGCCC‑GCGGTGGGAATCGTAGTGGCGGGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCg                                        <  2:37685/228‑1 (MQ=255)
                                                                                                                                                                                                     ccccaaggcacca‑ACGC‑GGGGGGGGGGAAATTCCGAGAGGGCTTTCGGGAAAGTGGACGTTGGAGGACCCCC‑GCGACGGACATCGTATTGGCGGGGGGCAGGCCATGTATCATCGCGGTCGTGATGCCCAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTGTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCCGCT‑GACCTGCCg                                        <  2:549935/228‑1 (MQ=255)
                                                                                                                                                                                                     ccGCATTGGCACA‑AGGT‑GGCGGGGGGTAAATTCAGAATGGGTGTTTGGGTAACTGGAGCGTGGGCGACGCCG‑GCGATGGGCATCGTATGGGCGTGCGGCAGGCCATTTATCAGCGCGGTCGTGATGCACAGGCCAGCGGGAAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCg                                        <  2:297764/238‑1 (MQ=255)
                                                                                                                                                                                                     cagCAATGGCCCC‑ACGT‑AGCGGTGGTGAAATTCAGATAGGGCTGTTCGGTAACGGGACGGTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCg                                        <  1:889736/237‑1 (MQ=255)
                                                                                                                                                                                                     aCGCAATGGCACA‑ACGC‑TGGGGGGGTGAAATTCAGATAGGCTTGTTGGGTAACTGGACGTTGGACGAAGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGTTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCg                                        <  1:407289/239‑1 (MQ=255)
                                                                                                                                                                                                     aCGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCCCAGGCCAGCGGGCAACAGGGGGGCGAAGCCGGTCTGGCCGC‑CATGAAGGCGCTTTCAGATATGGGCCTTGAGCTTTCCATTACTGGCGGAGGTAACCCctgcctcttaaaa                                          >  2:330149/1‑230 (MQ=255)
                                                                                                                                                                                                     aCGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCGGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCg                                        <  1:346615/239‑1 (MQ=255)
                                                                                                                                                                                                      gtcaaagggcaacaCGC‑GGCGGGGGGAAATTTCAGAGGGGGATGTTGGGGAAGTGGTCGCGGGACCGGGCCA‑GCGACTGGCCTCGGATTGGGGGGCGGCAGGACATTTATCATCGCGGTCGGGATGCACAGGACAGCGGGCAACAGGGGGGCGAAGCCGATCTGGAACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCg                                        <  2:252678/226‑1 (MQ=255)
                                                                                                                                                                                                      ccccaaggccacacggC‑TGGGGGGGTGAATTTCAGAGTGGGCTTTTGGGTAACTGGCCGCGGGACGGCCCCC‑GGGACTGGCATCGTATTGGCGGGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCg                                        <  2:323597/224‑1 (MQ=255)
                                                                                                                                                                                                      cGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCCGCGGGAAACAGTGGGGCGGAGCCGATCTGGGCCGACAT‑AAAGCGCCTTACGATACAGGCCTGGAGCTTTCCATTACCGGGGG‑Gaagtaccccacagcactccacc                                        >  2:853507/1‑218 (MQ=255)
                                                                                                                                                                                                      cGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACATGGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGCTATCGGCCTTGAGCTTTCCCTTACTGGCgg‑gggttccccccctcacctccccc                                        >  2:804495/1‑217 (MQ=255)
                                                                                                                                                                                                      cGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGCCGTGATGCACAGGCCAGCGGGCAACGGTTGGGCAAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGAAATCGGCCTTGGCGTTTCCCTTAATggggg‑ggagaacacccgctgaccgcccg                                        >  2:123227/1‑217 (MQ=255)
                                                                                                                                                                                                           tGGCAAA‑ACGC‑TGCGGGGGTGAAATTCAAATAGGACTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCGCTGttt                                  <  1:353220/239‑1 (MQ=255)
                                                                                                                                                                                                                    cGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTg                                                                    >  1:659637/1‑199 (MQ=255)
                                                                                                                                                                                                                    cGC‑TGCGGGGGGAAAATTCAGATCGGGCTTGTCGGGAACTGGACGGTGGACGACGTCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCAGCGCGGTCGTGATGCCCAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTCCCCCTGCT‑GACCTGCCGCTGTTTAAAGatat                          <  1:635882/239‑1 (MQ=255)
                                                                                                                                                                                                                     cggtgggggggTGAAATTCAGATAGGGCTGTTCGGTAACGGGACGCTGGACGGCGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCGCTGTTTAAAGatat                          <  1:804495/233‑1 (MQ=255)
                                                                                                                                                                                                                        tGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGGTCTGGCACG‑CCTGAAGGCGCTTTCAGAAATCGGCCTTGGGCTTTCCATTAATGGCGG‑GAAGACCCCTCCA‑GACCcgccgcggggtaaaaaaaacag                       >  2:420954/1‑223 (MQ=255)
                                                                                                                                                                                                                                tGAAATTCAGATAGAGGTTGTCGGTAACTGGACGCGGGACGCCGCCC‑GTGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCGCTGTTTAAAGATATCCGCGTGaaa                <  1:55981/238‑1 (MQ=255)
                                                                                                                                                                                                                                tGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GGTTACCCCCGCT‑GACCTGCCGcc                                      >  1:94096/1‑215 (MQ=255)
                                                                                                                                                                                                                                tGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCGCTGTTTAAAGATATCCGCGTGaaa                <  1:570552/238‑1 (MQ=255)
                                                                                                                                                                                                                                ggAAATTCAGATAGAGTTGTTCGGTAACCGGACGGTGGACGACGGCC‑GCGACTGGCAGCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTGCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCGCTGTTTAAAGATATCCGCGTGaaa                <  1:373986/237‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaTTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCCGCT‑GCCCTGCCGCTGTTTAAAACAATCCCCTGGAAAGCg             >  1:173888/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGatat                                                                                           >  2:783576/1‑158 (MQ=255)
                                                                                                                                                                                                                                       cAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGatat                                                                                           <  1:783576/158‑1 (MQ=255)
                                                                                                                                                                                                                                              agCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTCCTGGCGG‑AGTGTCCCCCGCTGGACCGCCCCGTTTTTAAAGATATCCCGGGGAAAGGGTTTCTTggggg  >  2:718856/1‑235 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
AGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGCGCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACA‑ACGC‑TGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCC‑GCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACG‑CATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGG‑GATTACCCCTGCT‑GACCTGCCGCTGTTTAAAGATATCCGCGTGAAAGCGTTTATTGCCGG  >  NC_000913/3748674‑3749147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A