Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F9 I0 R1	131	136.1	2723430	98.1%	2671684	229.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,091,185	T→G	24.0%	Y394S (TAC→TCC)	frvB ←	putative PTS enzyme, IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,091,185	0	T	G	24.0%	149.9 / 5.3	83	Y394S (TAC→TCC)	frvB	putative PTS enzyme, IIB component/IIC component
Reads supporting (aligned to +/- strand): ref base T (21/35); new base G (22/2); total (45/38)
Fisher's exact test for biased strand distribution p-value = 5.58e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATC  >  NC_000913/4090948‑4091414

ttCGCTTTGGGGAGTAACCACAGTCCGTTCAGGACGAGAATGAATGAAAGGTTCGGCGCGAGGCCAATGCCCATGCCCAGAAAAACCGAACCCACCGGCGCCATCAGGGCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGTCCCGGCCGTTTGATTCCG‑AAAGCGTTCACCAGGC‑CGCCTGTAAGGCCGCCTGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGt                                                                                                                                                                                                                                              <  2:158451/238‑1 (MQ=255)

        gcggcgTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGGAAA‑GGGGCCGCCAGAGCGAAA‑GGaaaa                                                                                                                                                                                                                                     >  1:1075013/1‑236 (MQ=255)

         cggcgAAACCACAATCCTTCCAGGACGATAAGGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGAAAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGTGCCAGACGTTTGATCCCG‑AAGGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAg                                                                                                                                                                                                                                    <  2:603093/238‑1 (MQ=255)

          ggcgTAACCCCAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGCCGTTTGATTCCG‑AAGGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGc                                                                                                                                                                                                                                   <  1:1034692/238‑1 (MQ=255)

             gTACCCCCAATCCTTCCAGGAGAATAATGATTGAAATGTTCCCCCCGCGGCCAACCGCCAGCCCCAAAAAAAACGAACCCCCCGCGGACACCAGCCCGATCGGCGGTTCAAAAATCCCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGACCACCAGCA‑CGCCGGAAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcggt                                                                                                                                                                                                                               <  2:993940/239‑1 (MQ=255)

              tagtcacagcccgtgcAGGAGGATAAGAAATGACATGTGCAGCGCGCAGTCAATCACCAGCACCACAAAAAACGACCCCGCCGGCGACTTCAGGCCGACCAGCGGGTCAAAGATCCCCAACCCA‑GGTGCCAGGCGTGGGACCCAG‑AACGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcgctt                                                                                                                                                                                                                              <  2:462007/224‑1 (MQ=255)

              tAACCACAATCCTTTCAGGACGAAAAGGAAGGAAATGTTAAGCGCGAGGCAAACCGCCAGCACCAGATAAAACGACCCCACCGGCGACATCAGCCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGGGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcggt                                                                                                                                                                                                                               <  2:360662/238‑1 (MQ=255)

              gaacccgaatcctcgagacacaaTAATGAAAAAAAGGTTCAGCGCCAGCCACACCGCCAGCACCAGATAAAACGACCCCACGGGCACCATCAGCCCGAGACGCGGGCCAAATATCCACAAACAA‑GGTGCCCGCCTTTTGAGTCCG‑AAAGCGACCACCAGCA‑CCCTGGAAAGCCTGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcggt                                                                                                                                                                                                                               <  2:950335/216‑1 (MQ=255)

                     accctgtccatgagtataataAATAAAATTCTCACCCCGAGGCAAATCCCCGCCACCAGATAAAACGACCCCACCGTCTACTCCCTGCCACTCAGCGGGTAAAAGTTACCCAAACCA‑GGTTCCAGACGTTTGATCCCG‑AAAGCGATCCCCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGc                                                                                                                                                                                                                       <  2:424796/224‑1 (MQ=255)

                     aaTCCTTCCAGGACGTAAATGAAGAAAATGTTCAGCGGGGGGCCAATCCCCAGCACCAAATAAAACGAACCCACCGGCGAAAACCGGCCGATCCGCGGGTAAAAATCACCCAAACCA‑GGTCCCGGCCGTTGGATTCCG‑AAAGCGATCCCCGGCA‑CGCCGTTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGc                                                                                                                                                                                                                       <  2:1127668/239‑1 (MQ=255)

                      aTCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAC‑GGAGCTGCCCGGGGGGAA‑GGAAAAACcgggtcgggttgg                                                                                                                                                                                                                      >  2:86710/1‑234 (MQ=255)

                          tttCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGCAATCATCGGCAAC‑AGGACTGCCAGCGCGTAA‑GGAAAAGCCGATTCTGTTGCG‑GCtg                                                                                                                                                                                                                 >  2:761677/1‑239 (MQ=255)

                           ttCGGGACGAAAAGGAATGAAATTTCCAGCGCGAGGCCAATCGCCAGCCCCAGAAAAAACGAACCCCCCGGCGACATCAGGCCGATCAGCGGGTCAAAGATCCCCAACCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGt                                                                                                                                                                                                                                              <  2:153950/211‑1 (MQ=255)

                            tCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGCGCCGCCCGAGCGGAA‑GGAATAAACAGTTCGGGTgaggccgtg                                                                                                                                                                                                                >  2:507642/1‑231 (MQ=255)

                                  cGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCAcc                                                                                                                                                                                                         <  2:130620/238‑1 (MQ=255)

                                   gATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAA‑CGAGCTGCCAGGGGGCAA‑GGAAGAGCCGGGTCGGCTGCG‑AGTGG‑GGCACCg                                                                                                                                                                                                        >  1:1043625/1‑238 (MQ=255)

                                   gATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATACTCGGCAAC‑GGAGGTGCCCGAGCGGAA‑GGAAAAGCCGGTTCGGTTGCG‑GCCTT‑GGCCCCg                                                                                                                                                                                                        >  1:1062046/1‑238 (MQ=255)

                                     tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGc                                                                                                                                                                                                                                   >  1:153950/1‑211 (MQ=255)

                                     tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGGCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATAAACCGCAAC‑GGAGACGCCGGAGCGTAA‑GGAATAGCCGGTTCGGGTGCG‑GCAGT‑gggcccagac                                                                                                                                                                                                     >  2:406278/1‑231 (MQ=255)

                                       atgaatgaAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACcac                                                                                                                                                                                                   <  1:1058207/239‑1 (MQ=255)

                                       agaaaagaaattcacAGCGAAAGGCCAGTCCCCAGCACCAAATAAAGCGAACCAGCCGGCACAAACAGCCCGAACAGCGGGTCAAACATACCAAAACCA‑GGGGCCAGCCGTTTGTTCCCA‑AAAGCGACCCCCACCA‑CGCCGGTAAGGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACcac                                                                                                                                                                                                   <  2:935692/225‑1 (MQ=255)

                                           atgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATg                                                                                                                                                                                               <  1:897649/239‑1 (MQ=255)

                                              aaaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTAAAAGATCCCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATgg                                                                                                                                                                                              <  1:1155817/237‑1 (MQ=255)

                                               aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAA‑GGAGCTGCCCGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCa                                                                                                                                                                                            >  1:431799/1‑238 (MQ=255)

                                               aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGAAATCATCGGCAAC‑GGAGCTGCCCGAGCGGAA‑GGAAAAGCCGGGTTGGGTGGG‑ACTGT‑GGGGCCCACcacacatgga                                                                                                                                                                                            >  1:512837/1‑232 (MQ=255)

                                               aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCACGCCAGCGTATTGGCGGCACGCATCCGCGAC‑CGAGCTGACCGAGCGGAAGGGAAAACCCGGTT‑GGGTGCG‑ACTGG‑GGCGCCGGCCACACGGGCa                                                                                                                                                                                            >  2:269630/1‑238 (MQ=255)

                                                  gTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGCAATCATCGGCAAA‑GGGGCCGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAAAGGCacta                                                                                                                                                                                         >  1:856099/1‑238 (MQ=255)

                                                    tCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAAACATCGGCAAA‑GGAGCTGCCCGGGCGTAG‑GGAAAAGCCGGGTGGGTTGGG‑GCCGT‑GGCACCGGACAAAATgggccaaaag                                                                                                                                                                                      >  1:1097077/1‑231 (MQ=255)

                                                     cAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCAGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTTCG‑ACTGG‑GGCCCCGAACACAATGGGACCACTg                                                                                                                                                                                      >  1:1323707/1‑238 (MQ=255)

                                                      aGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAAACATCGGCAAC‑GGAGCTGCCCGCGCGGAA‑GGAATAGCCGGTTCGGGTGGG‑GCTGT‑GGCACCGACCACAAAGGCACTACCgg                                                                                                                                                                                     >  2:278101/1‑238 (MQ=255)

                                                        cgcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCCAC‑GGAGCGGCCAGAGCGTAA‑GGAAGAACCGGGTCGGGTGGG‑ACCGT‑GGGACCGGCCCCCAAGGCCCTCCTGGCa                                                                                                                                                                                   >  2:754034/1‑238 (MQ=255)

                                                        cgcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCAC‑GGAGCTGCCAGAGCGGAA‑GGAAAAACCGGGTCGGGTGCG‑GCCGG‑GGGCCCGCACACAAAGGCACCACTGGCa                                                                                                                                                                                   >  2:1155817/1‑238 (MQ=255)

                                                         gcaagccaaaTCGCCAGCCCCAGAAAAAACGCACCCCCCGGCGACAACAGGCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGACCACCAGCC‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAg                                                                                                                                                                                  <  2:1300141/230‑1 (MQ=255)

                                                             ggCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAA‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGGG‑ACTGT‑GGCACCGACCACACAGGGACTACTgggcgccact                                                                                                                                                                             >  1:1168749/1‑231 (MQ=255)

                                                                    cgcaagacccaaaaaaaaCGAACCCCCCGGCGACATCAGGCCAACCAGCGGGTCAAAGATACCAAAACAA‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGCTCACAAGCC‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCt                                                                                                                                                                      <  1:1253804/225‑1 (MQ=255)

                                                                    cGCCAGCACCAGATAAAACGAACCCGCCGGCGACATCAGGCCGACCAGCGGGTCAAAGACACCCAAACCA‑GGTGCCAGACTTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCt                                                                                                                                                                      <  2:1054994/239‑1 (MQ=255)

                                                                      ccacccccAGATAAAACGACCCCACCGGGGACATCGGGCCGACCGGGGGGTCAAAGATCCCCAACCCA‑GTTGCCAGACGTTTGATCCCA‑AAAGCGACCCCCACCA‑CGCCGTTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGt                                                                                                                                                                    <  2:512837/233‑1 (MQ=255)

                                                                       cAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAAAGGCACCACTGGCAGCCTCTTTTTCCTGTc                                                                                                                                                                   >  1:280667/1‑239 (MQ=255)

                                                                        aGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCCGAGCGGAA‑GGAATAGCCGGTTTGGGTGCG‑ACTGG‑GGCACCGACCCCAATGGGACTACACGCAGCCTCTTTTTcccttcg                                                                                                                                                                  >  2:175343/1‑234 (MQ=255)

                                                                         gCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATTCTCCGCCAC‑GGAGCTGCCAGAGCGGAA‑AGGATAACCGGGTCGGTTGCG‑ACTGG‑GGCAACGGCCACAAAGGCACTACTGGCAACCTCTTTTTCCTGTCgg                                                                                                                                                                 >  2:594096/1‑239 (MQ=255)

                                                                           acaaGAAAAAACAAACCCACCGGCGACATCAGGCCAATCGGCGGGTAAAAGAT‑CCCAAACCAGGGTGCCAGACGTTTGATCCCG‑AAAGCGATCCCCAGCA‑CGCGGGTAAGGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCg                                                                                                                                                                  <  2:569369/233‑1 (MQ=255)

                                                                            ccAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTa                                                                                                                                                              <  1:603688/239‑1 (MQ=255)

                                                                               gATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAC‑GGAGCTGCCAGAGCGGAA‑GGAAAAACCGGTTCGGTTGCG‑ACTGT‑GGCACCGCCCACCATGGCACTAATGGCAGCCTCCTTTTTCTGTCGGGTAAAc                                                                                                                                                           >  1:670224/1‑239 (MQ=255)

                                                                               caaaaaaCGAACCCCCCGGAGACAGCAGGCCGCACAGGGGGTAAAAGATCCCCAACCCG‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                                                                                                                                           <  2:1062046/236‑1 (MQ=255)

                                                                                 tAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                                                                                                                                           <  1:618769/237‑1 (MQ=255)

                                                                                        aaCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCAC‑GGGGCTGCCAGGGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCGCCGACCACCAAGGCACCACTCGCCGCCCCCTTTTTCTGTCTGGTAAAAAGT‑TTCGgc                                                                                                                                                 >  2:314843/1‑239 (MQ=255)

                                                                                         aCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCCGCGTATTGGCGGCAATCATCGGCCACGGGACCTG‑CAGGGCGGAA‑GGAATAGGCCGGTCGGTGGGGCCCGGT‑GGC‑CCCACCCCAAAGGGCCTACCGGCAGCCCCTTTTTCCTGTCgggggagctgtttgagc                                                                                                                                                  >  2:1283039/1‑224 (MQ=255)

                                                                                         aCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGgc                                                                                                                                                 >  1:368111/1‑238 (MQ=255)

                                                                                              ccGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAA‑GGAGCTGCCCGAGCGTAA‑GGAAAAACCGGGTCGGTTGCG‑ACTGT‑GGGACCGACCACAATGGGAACACTGGCGGCCTCTTTTTTCTGTCGGGGAAACAGA‑TTTGGCGCAATg                                                                                                                                           >  2:529782/1‑239 (MQ=255)

                                                                                                ggCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGaa                                                                                                                                         <  1:86710/239‑1 (MQ=255)

                                                                                                   gACATCAGGCCGACCAGCGGGCCAAAGATACCCAACCCG‑GGTGCCAGAGGTTTGATCCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑g                                                                                                                                      <  2:724574/238‑1 (MQ=255)

                                                                                                     cATCAGGCCGATCAGCGGGTAAAAGATCCCCAACCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGAc                                                                                                                                                                                                      <  2:397133/174‑1 (MQ=255)

                                                                                                       tCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGGAAC‑GGAACTGCCCGAGGGGAA‑GGAATAACCGGGTCGGTTGCG‑ACAGTGGGCACC‑ACCACCAAGGACCAACCGGCAGCCCCTTTTTTCTGTACGGTGAACAGTGTTggggccaagtaatccgc                                                                                                                                    >  2:431288/1‑224 (MQ=255)

                                                                                                              caccggggggTAAAAGAT‑CCCAAAGCC‑GGTCTCAGGTCTTTTATTCCGAAAAGCG‑CCCCCAGCCCCGCCGTTAATCCCGCCCGCCGGCGTATTGGCGGTAATCATGGGCAAC‑GGAGCTCCCAGAGCGTAA‑GAAATAGCCGGTTCGTTTGCG‑ACTGT‑GGCCCCGACCACAATGGCACTCCTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TGGGGCGCAATGAAC‑GTCGCAA‑GACccg                                                                                                                         <  2:1291617/232‑1 (MQ=255)

                                                                                                               aTCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAA‑CGAGCTGCCAGAGCGGAA‑GGAATAGCCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGGCCCTTTTTTCCGTCGGGGAAACAGG‑TTTGGCGCAATGAAC‑ATCCCAA‑GAaccc                                                                                                                         >  2:742344/1‑237 (MQ=255)

                                                                                                               aTCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCa                                                                                                                                                                                            >  1:397133/1‑174 (MQ=255)

                                                                                                                    cGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTTGGCGCAATGAAC‑GTCGCAA‑AACCCGCcgcg                                                                                                                    >  1:938951/1‑238 (MQ=255)

                                                                                                                      ggTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATTCTCCGCAAC‑GGAGCCGCCCAAGCGTAA‑GGGAAAGACGGTTCCGCTGCG‑ACCGC‑GGCACCGACCCCAAATGCACCACCGGCAGCCTCTTTCTTCTGTCCGGTAAAACGA‑TCGGGCGCCATGACC‑GTCGCAC‑TACCcgccgcccac                                                                                                                 >  2:1060536/1‑235 (MQ=255)

                                                                                                                                 cccAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCAC‑GGAGCTGCCCGAGCGGAA‑GGAAAAGCCGGTTCGGGTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACCTGCAGCCCCTTTTTTCTGTTCGGTGAACAGG‑TTTGGCGCAAAGAAC‑GGCGCCA‑GACCCCCCCCGTCgggggggctga                                                                                                       >  2:1039856/1‑233 (MQ=255)

                                                                                                                                      aCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGG‑TTTGGCGCCATGAAC‑GTCGCCA‑GACCCGCCGCGACGGGCGGCATCAGcgcg                                                                                                  >  1:23870/1‑238 (MQ=255)

                                                                                                                                      aCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGGCGGTTCGGGTGCG‑ACTGT‑GGGACCGACCACAATGGCACTACTGGCAGCCTCCTTTTTCTGTCGGGGAAACAAG‑TTTGGCGCAAATAAC‑CGCGCCA‑AGCCCGGCGCGACGGGCGGCagacggcgg                                                                                                  >  2:65039/1‑230 (MQ=255)

                                                                                                                                                        tGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAAAAACCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCCCTTTTTTCTGTCGGGGAAACAGT‑TTGGGCGCAACGAAC‑GGCGCCA‑AACCCGCCCCGGCGGGCGGCCTCAGGCCGACAAAGcccgcgcagggg                                                                                >  2:1145576/1‑229 (MQ=255)

                                                                                                                                                               g‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGT‑TTGGGCGCAATGAAC‑GTCGCCA‑GACCCCCCCCGACGGGCGGCCTCCGCGCGACCACGCCGCCCATGGCGTACCac                                                                          >  2:68690/1‑237 (MQ=255)

                                                                                                                                                                 aagggcacccccAGCC‑CGGCGGAAGGGCCGCCCGCCAGCGTATTGGGGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                                                        <  1:68690/229‑1 (MQ=255)

                                                                                                                                                                 aaaGCGACCCCCGGCC‑CGCGGGTAGGCCCGCCCGCCAGCGTATGGGCGGTAATCACCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCGGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TGGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                                                        <  1:622097/239‑1 (MQ=255)

                                                                                                                                                                      gATCCCGAGCA‑CGCGGGTAATGCCCCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATaaa                                                                   <  1:742344/239‑1 (MQ=255)

                                                                                                                                                                               cA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCCGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGG‑TTGGGCGCAATGAAA‑GGCGCAA‑AACCCGCCCCGGCGGGCGGCAACACGGCGCACACCCCGCCCACGGCGGACCAGCCATAAAAGAGttttt                                                          >  2:1257764/1‑239 (MQ=255)

                                                                                                                                                                                 acgcggtaaaggccgccCGCCAGCGTTTTGGGGGTAAGCAGCGGCAAC‑GGGGCTGCCAGGGGTTAA‑GAAGTAGCGGGTGCGGTTGCG‑AGTGG‑GGCACCGCCCAAAATGGCCATACTGGCAGCCTCTTTTCCCTGTGGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTc                                                         <  2:1142804/228‑1 (MQ=255)

                                                                                                                                                                                     cGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTTCTGTCGGGGAAAAAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc                                                     >  1:342983/1‑239 (MQ=255)

                                                                                                                                                                                               gcccgccAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGGACCAGTCATAAATGTGTTTTTTCAGCAGTGAGAAGc                                           >  1:349611/1‑239 (MQ=255)

                                                                                                                                                                                                cccgccAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCCCAATGGCACTACTGGCAGCCTCTTTTTCCTTTCGGGGAAACCGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCCTAAATGTGTTTTTCCAGCAGTGCGAAGCa                                          >  1:354151/1‑239 (MQ=255)

                                                                                                                                                                                                 ccgcgggggTATGGGGGGAAACCGTCGGCAAC‑GGGCCTGCCAGGGCGTAA‑GGAATGGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCCCTACTGGCAGCCTTTTTTTCCTGTCGGGAAAACAGT‑TTGGGCGAAATGAAC‑GCCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAGGCag                                         <  2:670224/231‑1 (MQ=255)

                                                                                                                                                                                                  cgccAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCCGAGCGGAA‑GGGAAAGCCGGTTCGGTTGCG‑ACCGT‑GGCACCGAACACAATGGCACTACTGGCCGCCTCTTTTTCCTGTCGGGGAAACAGG‑TTGGGGGCAAAGAAA‑GTCGCAA‑AACCCGCCGCGGCGGGGGGGCTTCGAGCGGCAACGCCGGCCGTGGCCGAACAGGCATAAAAGGGTGTTTCccgcaagggggaggagg                                        >  2:841083/1‑227 (MQ=255)

                                                                                                                                                                                                          ttttGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGAAA‑GAAATAGCCGGTTCGTTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCa                                 <  1:1232954/236‑1 (MQ=255)

                                                                                                                                                                                                           tttcgggtaaagcatcGGCAAC‑GGAGGTGCCAGTGCGTAG‑GGAAGGGCCGTTGCGTCGGCG‑CCTGG‑GGGCCCGACCAAAATGGCACTACTGGCGCACTTTTTTTCCGGCGGTGGAACCGGT‑TTGGCCGCATATAGC‑G‑GGCAACGACCCGCCGCGACGGGCGGCATCAGCGCGACAACTCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAGCCAgg                               <  2:1043625/227‑1 (MQ=255)

                                                                                                                                                                                                           agtGGCGGAAATCACCGGCAAG‑GGGGCTGCCAGGGCGGAA‑GAAAAGGCGGGTTCGGTGGCG‑ACTGT‑GCCCCCACCCAAAAGGGCCCTACGGGCGCCTTCTTTTCCCTGTGGGAAAAACAGT‑TGGGCCGAAATGAAC‑GTCGCAA‑GCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg                               <  2:288620/237‑1 (MQ=255)

                                                                                                                                                                                                           aTTGGCGGTAATCATCGGCAAC‑GGGGCTGCCAGAGCGTAA‑GGAATGGCGGGTTCGGTTGCG‑ACTGT‑GGCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg                               <  1:754034/239‑1 (MQ=255)

                                                                                                                                                                                                           aTTGGCGGTAATCACCGCCAAC‑GGAGCGGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTGGCG‑ACGGT‑GGCACCGACCACAATGGCACTACTGGCGCCCTCTTTTTCCTGTCGGATAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg                               <  1:314843/239‑1 (MQ=255)

                                                                                                                                                                                                            tttagctgtaccaatcggacggggAGTTGCCAGAGGATAA‑GGAATAGCCGTGGCTGTGGCG‑CGTGT‑GGCACCGACCACAATGGAACTACTGGCACCCTCTTTTTCCTGTGGGGAAAACAGT‑TGGGGCGCAATGAAC‑GTCACAG‑TGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGATCCAgg                               <  2:469573/217‑1 (MQ=255)

                                                                                                                                                                                                                   gaaTCACCGGCAAC‑GGAGCGGCAAGAGCGGAG‑AGAAAAGCCGGTTCGGTGGCG‑ACTGT‑GGCACCGACCACAAGGCCACTTGTGGCAGCCTCTTTTCCCGGTCGGGTAAACAGT‑TTGGGCG‑AAAGAACGGCCGCAA‑GCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGtttt                        <  2:703682/237‑1 (MQ=255)

                                                                                                                                                                                                                       cctcGGCACG‑GGAGCTGCAAGAGGGTAA‑GGAAGAGCGGGTGCGGTGGCG‑ACGGC‑GGCCCCGACCACAAGGGCACTACTGGCAGCCTCTTTTTCCTGCCGGTAAAACAGT‑TTGGGCGCAAGGACC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGa                   <  2:1304864/237‑1 (MQ=255)

                                                                                                                                                                                                                         tcGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTTGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATAGCGTCCCCGTCATCAATGTGTTTTTCCAGCAGTGTGAAGCCGAGGCACCAGGCGGATTTCTTGC‑Tg                >  2:603339/1‑239 (MQ=255)

                                                                                                                                                                                                                         tcGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑Tg                >  1:1194718/1‑239 (MQ=255)

                                                                                                                                                                                                                                    aGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGCTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTCTTGATTGGGgccgcc        >  2:528106/1‑238 (MQ=255)

                                                                                                                                                                                                                                        gCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGCCGGGCGGCATCAGCGCGACAACGCCGAAGATGGCGCACCAGTCATAAATGTGTTTTTCAAGCAGTGAGAAGCAGAAGAACCAGGCGGGTTTATTGT‑TGGGGCCCGCCATATc  >  2:1043736/1‑239 (MQ=255)

TTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATC  >  NC_000913/4090948‑4091414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A