Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F9 I0 R1 103 124.7 2465218 97.5% 2403587 230.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,288,256 A→C 11.6% N165T (AAC→ACC)  nrfA → nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,288,2560AC11.6% 261.5 / 2.1 102N165T (AAC→ACC) nrfAnitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base A (57/30);  new base C (2/13);  total (59/43)
Fisher's exact test for biased strand distribution p-value = 3.07e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCA  >  NC_000913/4288018‑4288481
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
cgcgATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑CTTGCCATaa                                                                                                                                                                                                                                   >  1:144450/1‑239 (MQ=255)
  cgATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAac                                                                                                                                                                                                                                  >  2:654755/1‑238 (MQ=255)
  cgATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAac                                                                                                                                                                                                                                  >  1:426708/1‑238 (MQ=255)
    aTTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACAcc                                                                                                                                                                                                                               >  2:1141629/1‑239 (MQ=255)
             aGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGGCCGGAAATCGT‑CAACAACTTAGGTTGGGCCG‑ATTGCCATAACACCGCCTCTCCa                                                                                                                                                                                                                      >  1:336656/1‑239 (MQ=255)
               ccGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCaga                                                                                                                                                                                                                    >  2:943216/1‑239 (MQ=255)
                         aTGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAGCAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCaaa                                                                                                                                                                                                          >  2:1030511/1‑239 (MQ=255)
                            cTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCCCCAAAGGc                                                                                                                                                                                                       >  2:2686/1‑239 (MQ=255)
                                tGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑CTTGCCATAACACCGCCTCTCCATAGTTCGCCAAAGGCaaa                                                                                                                                                                                                    >  1:133447/1‑238 (MQ=255)
                                 gCTGTGACTGTGGGGGGTAAACCCCGGCGTACCGGGGCGCCAAAAAAGGCTGAAGATGGTCCGCTCCCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGGGGCGCGTCTGACCCAGAAAGACGGAGAAGAGGGCTA‑CTTCCACGGTAAATGGGGGCGCGGCGGTCCGGAAATCGT‑AAACAACTTAGGTTGTGCCG‑ATTGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAAcc                                                                                                                                                                                                  <  1:960265/239‑1 (MQ=255)
                                     tgACCGATGTGCGTGAAACCCCGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGGTGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGGCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAg                                                                                                                                                                                              >  1:1000666/1‑239 (MQ=255)
                                      gACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAACGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑CTTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCgcggt                                                                                                                                                                                             >  1:400964/1‑235 (MQ=255)
                                        ccGAGGGGCGTGACACCCTGCGGACCGGGGGGCCAAAAAACGCTGAGTGTGGTCCGCTACCGAGGGATTGCGGGATTTGAAAACGCCCGGATGTGGGGCGTCTGATCCAAAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAAGGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTGGTTGGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTa                                                                                                                                                                                           <  1:784918/239‑1 (MQ=255)
                                           aTGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGCAGTTAAc                                                                                                                                                                                         >  1:1145023/1‑238 (MQ=255)
                                            tgtgCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGCGTTCGCCAAAGGCAAACCGGAGTTAAc                                                                                                                                                                                         >  1:1054372/1‑237 (MQ=255)
                                            tgtgCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAAc                                                                                                                                                                                         >  2:420716/1‑237 (MQ=255)
                                                cgtcaaaacccacctACCGGTGAGCCGAAAAACGCTGAAGATGGTCCGCTACCGATAGCATGCTGGAGTTGTACAAACACGGATGTGGCGCGTCTGCTACAGAAAGACGGCGACGTAGGCTA‑CTTTCACGGTAAATGGGCCCGCGGCGGTCCGGCAATCGC‑CAAAACCGCAGGATACGACG‑AATGACATACAACCTCCACTACACACTTCGCCAAGGGCAAACCGGCGTTAACACttt                                                                                                                                                                                    >  1:189483/15‑238 (MQ=255)
                                                  tGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGAAGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTccc                                                                                                                                                                                 >  2:359393/1‑239 (MQ=255)
                                                          tGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAATATGGCTA‑ATTCCACGGTAAATGGGCACGCGGCGGTGCGGAAATCGT‑CAACAAATTAAGTTGTGGCG‑ATTGCCCTAACACCGCATCTCCAGAATTACCCAAAGGCAAACCGGAGTGAACCCTTTCccgtccggta                                                                                                                                                                         >  1:131473/1‑236 (MQ=255)
                                                          tGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCACTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTAt                                                                                                                                                                         >  2:962311/1‑239 (MQ=255)
                                                           gCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTAt                                                                                                                                                                         >  2:1029598/1‑238 (MQ=255)
                                                            cGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATACAGAAAGACGGCGCAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGA‑CAACAACTTAGGTTGTGACG‑ATTGCCATAACACCGCCTATCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGc                                                                                                                                                                       >  2:677367/1‑239 (MQ=255)
                                                               aCCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCgg                                                                                                                                                                     >  2:711351/1‑238 (MQ=255)
                                                                 cGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTc                                                                                                                                                                  >  1:264748/1‑239 (MQ=255)
                                                                        ccGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGGAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGAt                                                                                                                                                           >  2:1042722/1‑239 (MQ=255)
                                                                          aaaaaaCGGTGAAGAGGGCCCGTCACCGAGGGCAGGGGGGAGTTGAAAAAGCCGGGATGGGGCGCGTCGGACCAAGAAAGACGGGGAAGAGGGTTA‑CTCCCCGGGTAAATGGGCGCGGGGCGGTCCGGAACTCGT‑AAACAACTTAGGTTGTGCCG‑ATTGCCAAAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATgg                                                                                                                                                         <  1:653642/238‑1 (MQ=255)
                                                                              aacgctgaggggtggccGCGACCGAGGGCATGAGGGACTTGAAAAACCCCGTGTGGGGCGCGTCTGATCAAGAAAGAGGGGGAAGATGTCGA‑CTCCCCGGGTAAATGGGCGGGGGGCGGTCGGGAAATCGT‑GAACACCTTAGTTTGTGCCG‑ATTGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGc                                                                                                                                                     <  1:245874/224‑1 (MQ=255)
                                                                                cGCTGAAGATGGTCCGCTACCGATGGCATGCGGGAGTTGAAAAAGCCCGGATGGGGCGCGCCTAATCCAGAAAGACGGAGAAGAGGGCTA‑CTCCCACGGTAAATGGGCGCGCGGCGGCCGGGAAATCGC‑AAACACCGTAGGTTGTGCCG‑ATTGCCAAAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCa                                                                                                                                                   <  1:517900/239‑1 (MQ=255)
                                                                                     aaGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGt                                                                                                                                              >  2:457230/1‑239 (MQ=255)
                                                                                      aGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTa                                                                                                                                             <  2:1084460/239‑1 (MQ=255)
                                                                                                 gacggatgcacggcgggggtggTAAAAGGCCGAGTGGGGCCGGTCGGACCAAGAAGGCCGGGGAAG‑TTGCTACCTCCCACGGTAAAGGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTGGGTGGTGCCG‑ATGGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTgag                                                                                                                                  <  1:651388/218‑1 (MQ=255)
                                                                                                 ctcagtttgcggtgtGGAGTTGTAATAGCGCGGGTGCGGCCAGACTGATCCAGAAAGGCGGCGAAGGTGTCTA‑CTTCCAGGATATGGGGGCGCGGTGGGGCCCAGAAGTCGC‑CAGAATCTTGTTTGGCGCGG‑ATATTCATAACCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAGCCTTTTgag                                                                                                                                  <  1:254501/225‑1 (MQ=255)
                                                                                                      aTGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑GTTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAg                                                                                                                              >  2:466470/1‑238 (MQ=255)
                                                                                                      aTGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGATAAAg                                                                                                                              >  2:282344/1‑238 (MQ=255)
                                                                                                        ggCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGC‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAAGCAAACCGGAGTTAACCCTTTCCCGTCCGTCTGCGGCTCGCGCGATGGAAGCCATTGGTAAACCATTTAAGAAAGCCg                                                                                                                           >  1:50129/1‑239 (MQ=255)
                                                                                                           aTGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGAc                                                                                                                        >  2:296526/1‑239 (MQ=255)
                                                                                                             gCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGt                                                                                                                      >  2:491818/1‑239 (MQ=255)
                                                                                                             gCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGG‑CAACAACTTAGGTTGTGCCG‑ATTGCCAGAACACCGCCTCTCCAGAGTTCGCCAAAAGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGCAAGCCGGACGt                                                                                                                      >  1:352286/1‑239 (MQ=255)
                                                                                                                          aggccggtaTGTGACGCGTCGGTGCAAAAAAGACGGGGAAGATGGTTC‑CTCCTAGGGTAAGGGGGGGCGCGGCGG‑CCGGAAAATGTAAAACAACTGGGGTGGCGCCT‑ATGGCCAAAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAAt                                                                                                         <  1:1042851/231‑1 (MQ=255)
                                                                                                                                 gATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCCAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTAATCGGCTCGCGCGGTGGAATCCATTGGTAACCCTTTTGAGAAAGCCGGACGCGTCGACCACCAATCGATGGt                                                                                                  >  1:934855/1‑239 (MQ=255)
                                                                                                                                 gATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTc                                                                                                                                                                                   >  2:298873/1‑158 (MQ=255)
                                                                                                                                 gATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGt                                                                                                  >  1:1013179/1‑239 (MQ=255)
                                                                                                                                 gATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATAGAAGCCCTTGGTAAACCTTTTGAGAAAGCCGGACGGTTCGACCAGCAATCGATGGt                                                                                                  >  1:689482/1‑239 (MQ=255)
                                                                                                                                    ggggggcgTCTGAGCCAGAAAGACGGAGAAGATGCCTA‑CTACCGCGGTAAATGGGCGCGCGGCGGTCCGAAAATCGC‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGttt                                                                                                <  1:2686/233‑1 (MQ=255)
                                                                                                                                     tgGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGc                                                                                              >  1:154956/1‑239 (MQ=255)
                                                                                                                                      ggCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAACGGCAAACCGGAGGTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGCAACCCTTCGGAAATCTTTTGGGAAAGCCGGCGGTTTCGACCAGCAATCGATGGTTTTCg                                                                                             >  1:210437/1‑239 (MQ=255)
                                                                                                                                       gcgcGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCCCGCGCGATGGAAGCCATTGGTAAAACTTTTGAGAAAGCCGGGCGTTTCGCCCAGCAATCGATGGTTTGCgg                                                                                            >  1:1181577/1‑239 (MQ=255)
                                                                                                                                         gcgTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGt                                                                                           <  2:426708/238‑1 (MQ=255)
                                                                                                                                             cggacccAGAAAGACGGCGAAAATGCCTA‑CTCCAACGGGAAAGGGGCGGGCGGCGGTCCGGAAATCGT‑CAACACCTTTGGTTGTGCCG‑ATTGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGt                                                                                       <  1:780146/233‑1 (MQ=255)
                                                                                                                                                acccAGAAAGGCGGCGAGTAGGGCTA‑CTTCCGCGGAAAAGGGGCGCGGGGCGGTCCGAAACTCGT‑CAACAACTTAGGTTGGGCCG‑ATTGACAAAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGcc                                                                                    <  1:887448/236‑1 (MQ=255)
                                                                                                                                                aTCAAGAAAGGCGGGTAAGAGGTGTA‑CTTCCAGGGTAAATGGGCGCGCGGCGGTCCGAAATGCGT‑CAACACCTTAGGTTGCGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGcc                                                                                    <  1:1061748/238‑1 (MQ=255)
                                                                                                                                                  ccAAAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtg                                                                                 <  2:522701/239‑1 (MQ=255)
                                                                                                                                                     gaaaagcGGGGAATATGGCTA‑CTTCCCGGGTAAAGGGGGGCGCGGCGGTCGGAAAACGGT‑CAAAAACTTGGGGTGGCCCG‑ATTGCAAAAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtgtg                                                                               <  1:879948/232‑1 (MQ=255)
                                                                                                                                                      aaaGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGa                                                                             >  2:219537/1‑239 (MQ=255)
                                                                                                                                                       aaggcggtaaaGATGGTCT‑CTTCCCCGTGAAGTGGGCGCGGGGCGGTCCGGAAATCGT‑CAACTACTGAGGTTGTGCGG‑ATTGCCATACCCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtgtg                                                                               <  1:126258/227‑1 (MQ=255)
                                                                                                                                                          aCGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTAt                                                                         >  1:1075576/1‑239 (MQ=255)
                                                                                                                                                                  agggggctcgtcccACGGTAAAGGGGCGCGGGGCGGTCCGGAAATCTC‑CAACAATTTAGGTTGGCCCG‑ATGGCAATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCg                                                                  <  2:1054372/227‑1 (MQ=255)
                                                                                                                                                                            ttCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCaaaa                                                         >  2:684737/1‑238 (MQ=255)
                                                                                                                                                                                 cGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAg                                                   <  2:264748/239‑1 (MQ=255)
                                                                                                                                                                                 cGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGGTTGCGGTCCGTGCCATGAGGAGTATTACTTCGACGGCAAAAACaaa                                                    >  1:998243/1‑238 (MQ=255)
                                                                                                                                                                                  ggTAAAGGCGCGCGCGGCGGTCCGAAACGCGA‑CAACAACTGAGGTTGTGCCT‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGc                                                  <  1:1034611/239‑1 (MQ=255)
                                                                                                                                                                                   gTAAAGGGGGGCGCGGGGGCCGGGAAATCGT‑CACCACTTGAGGTTGTGCGA‑ATTCCCAAAACCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACaaa                                                    <  2:270812/236‑1 (MQ=255)
                                                                                                                                                                                          ggCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                           <  2:159945/238‑1 (MQ=255)
                                                                                                                                                                                          gccgcgcgGGGGCCCGGAAA‑CTT‑CAACCACTTAGTGTTTGCCGCTTTCCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                           <  1:1190388/236‑1 (MQ=255)
                                                                                                                                                                                            cgcgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAAtt                                         >  1:594646/1‑238 (MQ=255)
                                                                                                                                                                                            cgcgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAAtt                                         >  2:90216/1‑238 (MQ=255)
                                                                                                                                                                                             gcgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ACTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGACGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAATCTAAGCGGTTAACTTc                                        >  1:1185505/1‑238 (MQ=255)
                                                                                                                                                                                              cgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTccc                                      >  2:659993/1‑239 (MQ=255)
                                                                                                                                                                                              cgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTccc                                      >  1:428283/1‑239 (MQ=255)
                                                                                                                                                                                              cgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTccc                                      >  1:747658/1‑239 (MQ=255)
                                                                                                                                                                                              cgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTccc                                      >  1:9362/1‑239 (MQ=255)
                                                                                                                                                                                              cgcgGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGAGCCG‑GTTGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATCCGGCTCGCGTGCTGTAAGCCACTGGTCAACCTTTTGTGAAAGCCGCCCGTTTCGCCCAGCAATCGATGGTTTGCGCTCAGTGCCATGTGACGTAGTACTTCCACGGCACAAACAAAACGGTTAAATTccc                                      >  1:652229/1‑239 (MQ=255)
                                                                                                                                                                                               gcggggGCCCAGAAATCGT‑CAACATCTTGGGTTGTGCCG‑ATTGCCATAACCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCg                                     <  2:1118293/239‑1 (MQ=255)
                                                                                                                                                                                               gcggcgGTCCGAAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCg                                     <  2:1013179/239‑1 (MQ=255)
                                                                                                                                                                                               gcggcgGCCCGGAAACCGT‑CAACAACTTAGGTTG‑GCCGAATTGCCATAACCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCg                                     <  1:296526/239‑1 (MQ=255)
                                                                                                                                                                                               gcggcgGCCCGAAAATCCT‑CAACAACTTAGGTTGTGCGG‑ATTGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCg                                     <  2:566961/239‑1 (MQ=255)
                                                                                                                                                                                                cggcggTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGt                                    >  2:326288/1‑239 (MQ=255)
                                                                                                                                                                                                                gT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGaa                    >  1:253125/1‑239 (MQ=255)
                                                                                                                                                                                                                 gcaaacaatTTAGGTTGGCCCG‑ATTGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGaa                    <  2:163945/236‑1 (MQ=255)
                                                                                                                                                                                                                   caacaaCTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAg                  >  2:457851/1‑239 (MQ=255)
                                                                                                                                                                                                                    aacaccTTGGGTTGTGCCG‑ATTGCAATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGt                 <  2:664703/239‑1 (MQ=255)
                                                                                                                                                                                                                     cacaacggagttcggcccaaTTCCCAGACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAGCCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGt                 <  2:817330/220‑1 (MQ=255)
                                                                                                                                                                                                                       attttgggTTGTGCCG‑TGGGCAAAACCCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGa              <  1:801902/233‑1 (MQ=255)
                                                                                                                                                                                                                       aacTTGGGTTGTGCCG‑ATTGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGa              <  2:546297/239‑1 (MQ=255)
                                                                                                                                                                                                                       aacTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGa              <  2:336656/239‑1 (MQ=255)
                                                                                                                                                                                                                       aacTTAGGTTGGCCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGa              <  1:327120/239‑1 (MQ=255)
                                                                                                                                                                                                                        aactgaggtggcgacgcctccaaTAACACCGCCTCTCCAGACTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGa              <  1:1141629/217‑1 (MQ=255)
                                                                                                                                                                                                                            ggtttGTGCCG‑ATCGCCATAACCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAata         <  1:282344/236‑1 (MQ=255)
                                                                                                                                                                                                                                 gtgCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAATCGGAGTTAACCCTTTCGCGTCCGTATGCGGCCCGCGCGAAGGAAGACCTTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATAGATGGATCGCGGTCAGTGCCATGTGGAGAATTACTTCGACGGCAAAAACAAAGCGGTTAAATTACCGTGGGACGACGGCATGAAAGTCGaacgtttggcg    >  1:99439/1‑230 (MQ=255)
                                                                                                                                                                                                                                 gtgCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGa     >  2:801902/1‑238 (MQ=255)
                                                                                                                                                                                                                                 gtgCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAg    <  2:154956/239‑1 (MQ=255)
                                                                                                                                                                                                                                  tgCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc   >  2:346511/1‑239 (MQ=255)
                                                                                                                                                                                                                                  tgCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc   >  1:836304/1‑239 (MQ=255)
                                                                                                                                                                                                                                  gtcacgtttGACAAAACACCGCCTCTCCAGAGTGCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACATTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACTGCATGAAAGTCGAAAATATGGAg    <  1:677367/232‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc   <  2:747658/238‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCa  >  2:293541/1‑239 (MQ=255)
                                                                                                                                                                                                                                   gCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCa  >  2:1119991/1‑239 (MQ=255)
                                                                                                                                                                                                                                   cccG‑TTTGCCATAACCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGATAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTTCTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc   <  2:131473/237‑1 (MQ=255)
                                                                                                                                                                                                                                    cgctgtgggattAACCCCGCCTCTCCAGACTTGGCCAAAGGCAAACCGGAGTTAACCCATTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAGCCTTTTGAGAAAGCCGGACGTTTCGACCAGCAAACGATGGTTTGCGGGCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc   <  1:65616/227‑1 (MQ=255)
                                                                                                                                                                                                                                    cccgcttcccccaaCTCCGCCTCTCCAGAGTGCGCAAAAGCCAAACCGGAGTTAACCCTTTCGCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc   <  2:189483/226‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
CGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTA‑CTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGT‑CAACAACTTAGGTTGTGCCG‑ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCA  >  NC_000913/4288018‑4288481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A