Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F17 I0 R1	160	98.0	2214160	89.0%	1970602	222.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,043,013	A→G	22.4%	G200G (GGA→GGG)	srkA →	Cpx stress response Thr/Ser protein kinase, MazF antagonist protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,013	0	A	G	22.4%	153.4 / 8.9	76	G200G (GGA→GGG)	srkA	Cpx stress response Thr/Ser protein kinase, MazF antagonist protein
Reads supporting (aligned to +/- strand): ref base A (12/44); new base G (15/3); total (28/48)
Fisher's exact test for biased strand distribution p-value = 4.08e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGGAAGCGGTTGGGCGTTATTTAGGGC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGC  >  NC_000913/4042775‑4043249

gggttgAAGCGGTTGGGCTTTTTTGGGGGC‑GTA‑GCACCCAGCAGGGGCGCAAAACGTTTTTTTC‑CCAGCGCCCTCCCATCGGTTTAAAGGAATATCCC‑ATTGGGCCACGCAAGGTGTTTGG‑GGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACGGATGAGCTGTTTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGga                                                                                                                                                                                                                                                   <  2:849145/234‑1 (MQ=255)

    ggAAGCGGTTGGGCGTTATTTAGGGC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGAGGG‑CAGGATTCTCCGTCCTGCGGGTC‑CATGgtg‑aa                                                                                                                                                                                                                                               >  1:622803/1‑237 (MQ=255)

        gCGGTTGGGCGTTATTTAGGGC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGAGTTCACCGTTTTGCGGGTA‑CATGgag‑aagtgc                                                                                                                                                                                                                                           >  1:207702/1‑233 (MQ=255)

            ttGGGCGTTATTTAGGGT‑GTATCCA‑CCAGACGGGGCCCAAAACGTTTTTTAC‑CCATCCCCCGACCATCGGTTTGAAAGAAGATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTCCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑c                                                                                                                                                                                                                                      <  1:366209/238‑1 (MQ=255)

            ttGGGCGTTATTTAGGGC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCCCACTGGCGGGAAAGATTTC‑CCGTTCTGCGGCCT‑CAAGGGG‑ACTTcccagcc                                                                                                                                                                                                                                      >  1:227526/1‑234 (MQ=255)

            ttGGGCGTTATTTAGGGC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTTACCGTTTTTCGGCTC‑CAAGGGG‑ACTGCCAcc‑cc                                                                                                                                                                                                                                     >  1:843320/1‑239 (MQ=255)

            ttGGGCGTTATTGAGGGC‑GTTTGCA‑CCAGACGGGGCGCAAACAGCTTTTTCT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTT‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑c                                                                                                                                                                                                                                      <  1:381800/238‑1 (MQ=255)

             ggggCGTTA‑TTAGGGCGGTATGCA‑ACAGTCGGGGCTCAAAAAGCTTTTTAC‑CCATCGCCAGACAATGGGTTTGAACGAATTGCTC‑ATTGAGCCAGGCAAGCTGTTTGA‑GGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACATATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCg                                                                                                                                                                                                                                    <  1:1045981/238‑1 (MQ=255)

                gCGTTATTTAGGGC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑GAGAGTTTACCGTTTTGCGGCTC‑CACAGGG‑AGTGCCACG‑CCcggg                                                                                                                                                                                                                                 >  1:256538/1‑238 (MQ=255)

                           ggC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCCCACTGGCGGG‑CAGAATTCAACGTGCTGCGGCAA‑CCAGGAG‑GAACCCACG‑CAGGGAATAAtcttcgg                                                                                                                                                                                                                      >  2:15743/1‑235 (MQ=255)

                                tATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACCCTGGCGGG‑AGAAATTTACCGTACTGGGGCGACCATAGA‑‑ACAGCCACC‑CCGGGaaaaaatactgggggg                                                                                                                                                                                                                  >  2:382113/1‑225 (MQ=255)

                                tATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTAACCGTTCTCGGGGCA‑CATGGGG‑AGTGTCACG‑CCAGGAGAATTTTCTGCcgcg                                                                                                                                                                                                                  >  1:220150/1‑239 (MQ=255)

                                 ttggcccacgacgggtgccAAACTGTTTTTTAC‑CCCCCCCCCGACCGTGGGTTTGAACCGATATCGC‑TTGGCGCCACGCAACGTGTTTGA‑GGACGCTCCGCCGATTCCTGCCGGGTTGAAAGCGTCATTCCGAAAAGG‑GAAAGCTGGGCTGTGTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGcgcg                                                                                                                                                                                                                  <  2:227526/221‑1 (MQ=255)

                                 tgtcA‑CCAGGCGGGGGGCCAACCG‑TTTTTATACCTGCGCCCGACCGT‑GGGTTGAACAGAAATCCCTATTGGGCAACGAAGGCTTTTGGA‑GGACGCTACACTGTTCCCTACCGGTTTGAAAGCGGAATTCCGAAAAGG‑GAAAGATGGGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGcgcg                                                                                                                                                                                                                  <  2:757850/235‑1 (MQ=255)

                                 aTGCA‑CCAGATGGGTCGCAAACTGCTTTTTAT‑CCAGCGCCCGACCTTCGGTTTGAACGAATATCTC‑ATTGAGCCCCGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGa                                                                                                                                                                                                                 <  1:496735/239‑1 (MQ=255)

                                 aTGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTTTGCGGCCA‑CACGGGG‑ACTGCAACA‑CCCGGGGAATTCTCTgggtcga                                                                                                                                                                                                                 >  1:70152/1‑234 (MQ=255)

                                 aTGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCAACCTTCTGCGGCTA‑CATTGAG‑AATGCCCCC‑CCCGGGAGATTCTCTGGcgcgc                                                                                                                                                                                                                 >  1:1085401/1‑238 (MQ=255)

                                  tGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTTACCGGTCTGCGGCTA‑CATGGGG‑ACTGCCCCG‑CCGGGAATATTTTCTGGcgcgcg                                                                                                                                                                                                                >  1:1010910/1‑237 (MQ=255)

                                        caggcgggtccaaaaCAGCTTTTTTC‑CCATCGCCCGCCCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGTGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTcc                                                                                                                                                                                                           <  2:405626/227‑1 (MQ=255)

                                        cAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAAGATTT‑AACGGTCTGCGGGTA‑CCAGGGG‑ACTGCCCCC‑CCCGGGATATTTTCTgggcgccggggcc                                                                                                                                                                                                           >  1:853655/1‑228 (MQ=255)

                                         cgACGGGGCCAAAATCGTTTTTTCT‑CCATCGCCCGCACATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCa                                                                                                                                                                                                          <  2:256538/238‑1 (MQ=255)

                                              gggCCCAACCGGTTTTTTTC‑CCCTCCCCCGGCCTTGGGTTTGAACGAATTCCCC‑TTTGGCCCACGCAAGCTGTTTGG‑GGACCCTATACTGATCCCTTCCGGGTTGAAAGCGTCATTCCGGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑t                                                                                                                                                                                                       <  2:207702/236‑1 (MQ=255)

                                               ggCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AGAAATTCACCCTTTTTCGGCCC‑ACTGGGG‑ACTGCCCCC‑CCGCGGATATtctcctgtcgggagggccaacgatt                                                                                                                                                                                                    >  2:605695/1‑218 (MQ=255)

                                                gcgcAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑GAAAATTTACCCTTCTGCGGCTA‑CATGGGG‑ACTGCCACG‑CCCGGGGAATTTTCCGGCGCGCTGGTTCAA‑tggttg                                                                                                                                                                                                  >  1:589702/1‑239 (MQ=255)

                                                   caaaacgCTTTTTAC‑CCATCCCCCGGCCATCGGTTTGAACGAATATCCC‑ATTGGGCCCCGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑tgtttgtt                                                                                                                                                                                                <  1:1025971/232‑1 (MQ=255)

                                                         gCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAAATTTCACCGCTCTGCGGCTA‑CATGGGG‑AATGCCACC‑CCCGGGCAATTTCCTGGGCCGACGGTCCAC‑TGTtttttgtctcgt                                                                                                                                                                                         >  1:319523/1‑233 (MQ=255)

                                                                 ttcccccgccctgccttggttttGAATGATTTTCTC‑TTTGAGCCACGC‑AGGTGTGTGAGGGCCGCTACACTGTCACCTTCGGGTTTGAAGGCGGCATTCCGGAAAGG‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTAACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGc                                                                                                                                                                                  <  2:495096/219‑1 (MQ=255)

                                                                 acccAGCGCCCGACATGCGGTTTGACCGAATATCTC‑ATTGAGCCACGAAAGCTGTTTGA‑GGACGCTACACGGATCCCTTCGGGTTTGAAAGCGGCATTCCTGAAAGG‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGc                                                                                                                                                                                  <  1:728063/237‑1 (MQ=255)

                                                                       cGCCCGACCTTCGTTTTGAACGAATATCTC‑TTGGAGCCACCAAGGTTTTTTGA‑GGCCGCTACCCGGATGCCTTCCGGGTGGAAAGCGGCATTCCTGAAGGC‑GACAGATGAGCTGATGGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTaa                                                                                                                                                                            <  2:319523/239‑1 (MQ=255)

                                                                          ccGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTGCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGa                                                                                                                                                                                                                                 <  1:463800/184‑1 (MQ=255)

                                                                               cctCGTTTTGAACGAATATCTC‑ATTGGCCCACGGAAGTTTGTGGA‑GGACGCGACATTGATCCCTCCTGGGTTGAAGGCGGCATGCCGAAAAGC‑GACAGTGTAGCTGATTGCCGCCGTTACAGCACCCTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAg                                                                                                                                                                    <  2:220150/237‑1 (MQ=255)

                                                                                     tttGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTCCACTGATCCCTTCGGGGTTGAAGGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTc                                                                                                                                                              <  1:446374/239‑1 (MQ=255)

                                                                                      ttGAACGAAATCCCC‑ATGGGGCCACGAAAGCTGTTGGA‑GGACGCTCCACTGATCCCTTCCGGGTTGAAAGGGGCATTCCTGAAAGC‑GACAGAGGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTc                                                                                                                                                              <  1:965985/238‑1 (MQ=255)

                                                                                        gAACGAATATCCA‑ATGGGGCCACGCAAGCTGTTTGG‑GGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑gg                                                                                                                                                          <  1:382113/239‑1 (MQ=255)

                                                                                              ttttctc‑TTTGACCCCCGCAAGCTGTTGTA‑GGACCCTACCTTGATTCCTTCGGGTTTGAAGGCGGCTTTCCGAAAGGC‑GACAGTTGAGTTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTtgt                                                                                                                                                    <  2:853655/236‑1 (MQ=255)

                                                                                                    taaTTGAGCCCGGCAAGGTGTTTGA‑GGACCCGAGACTGATGCCTTCCGGGTTGAAAGCGGCATTCCGGAAGGC‑GACAGATGAGCTGATGGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATg                                                                                                                                               <  1:409899/237‑1 (MQ=255)

                                                                                                        tGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTACTGAAAGC‑GACAGATGAACTGATTGCCGCCGTTACAGCACCCGGGCGGG‑AAACATTTAACGCTATGCGGGTA‑CAGAGAGAACTAGCACA‑CGGGGAAAATAC‑CTGCTGGCGTGGGCAAA‑TGTTTGTTGGAATGGATGAAGCACGTAATGGGGCCGCCGGCCC‑GGAGTTGTgggggtggg                                                                                                                                           >  2:185596/1‑232 (MQ=255)

                                                                                                          aGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑GAGATTTTACCGTTCTGCGGCTA‑AATTGGG‑ACTGCCCCG‑CCGGGGATATTTTCTGGGGCGCTGGGCCCA‑TGTTTGTTTATCTGGATGATGGACGGAATGGTCCAGCCGTTCA‑GGATTTTTGGGTG‑TTGTTc                                                                                                                                        >  1:167459/1‑239 (MQ=255)

                                                                                                                         ttGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑GAGATTTTACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTTTCTGGCGCGATTGTTCAA‑TGTTTGTTTGTCTGGGTGATGCACGGAATGGTCCAGCCGTTCA‑GGGTTTTTTGATG‑TTGTTCAATGGGGTTAAAAcc                                                                                                                         >  1:829308/1‑239 (MQ=255)

                                                                                                                               gACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTTACCGTTCTGCGGCTA‑CATGGGG‑ACTGCCACG‑CCGGGGATATTCTCTGGCGCGAGGGGCCAA‑TGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCGTTCA‑GGATTTTTGGATG‑TTGTTCAATGGGGGTAAAAcccggca                                                                                                                    >  1:129724/1‑234 (MQ=255)

                                                                                                                               gACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGGATATTCTCTGGCGCGCTGGTCCCA‑TGTTTGTTTATCTGGATGATGCACGTAATGGTCCAGCCGTGCC‑GGGTTTTTGGGTT‑TTGTTCAAAGGCGCTAAAGCCCagca                                                                                                                    >  1:217957/1‑239 (MQ=255)

                                                                                                                                aCGCTACACTGATCCCTGCCGGGTTGAATGCGTCATTCCTGAAGGC‑GACAGAGGTGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGagca                                                                                                                    <  1:948900/238‑1 (MQ=255)

                                                                                                                                 cGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAAAGTTTACCCTTCTTCGGCTC‑CCTCGAG‑ACTGCCCCGCCCGGGAATA‑TCTCTTTCCCGCTTGTCCAC‑AGTTTGTTGGTTTTGGTGATGGTCGCAATGGTGCAGCCCTTCA‑GGATTTTTGGGTG‑TTTCTCAAAGGCGATAAAAACGagccgc                                                                                                                  >  2:211421/1‑239 (MQ=255)

                                                                                                                                   cTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGAATTCTCCGTTCTGCGGGCC‑CATGGGG‑AATGCCCCC‑CCGGGGAAATTTTCTGTCGCGAGGGTCCCA‑TGGTTGTTGTTCTTGGGGAGGCACGGAATGGTCCCGCCCGCCAGGGTTTTGTTGGTG‑TGTCTCAA‑GGCGGTAAAGcagcgaaaggg                                                                                                                >  2:306249/1‑229 (MQ=255)

                                                                                                                                    ttcgcggATGCCTTCCGGTTTAGATGCGGCATTCCGGAAGGC‑GAATGATGAGCTGGTTGCCGCCGTTACAGCCACCGGGCGGG‑AAGATTTCCCCGTTCGGGGGCTA‑CATGGAG‑CCTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGa                                                                                                               <  2:217957/233‑1 (MQ=255)

                                                                                                                                    tACACTGATCCCTTCCGGGTTGAAAGCGGAATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTaa                                                                                                                                                                            <  1:1038332/180‑1 (MQ=255)

                                                                                                                                      cacgtaTACCTTCCGGGTTAAAAGCGGCATTCCTGAAAGC‑GACAGATGACCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATg                                                                                                             <  1:367201/234‑1 (MQ=255)

                                                                                                                                      cacTGCTCCCCTCCGGGTTAAAAGCGGCATCCCGTAAAGC‑AAGAGTGGGGCTGCTTGCCGGCGTTACAGCACCCTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATg                                                                                                             <  2:843320/239‑1 (MQ=255)

                                                                                                                                      cacTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGa                                                                                                                                                                                                                 <  1:378560/142‑1 (MQ=255)

                                                                                                                                         tGATACCTTCCGGGTTGAAAGCGGCATGCCGGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCa                                                                                                                                                                                 <  1:1068091/170‑1 (MQ=255)

                                                                                                                                            tACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGCG‑ACTGCCACG‑CCGGGGATATTCTCTGGCGCGATGGTGCAA‑tg                                                                                                                                                                                                      >  1:265452/1‑146 (MQ=255)

                                                                                                                                             cccTTGCGGTTGGAAAGGGGCA‑TCCAGAAAGCGGAAAGATGAGCTTAATGCCGACGTTACAACACACTGGCGGG‑AAGATTTCTCCGTTCTGCGTCTA‑CATGGCG‑ACTGCCAAG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTgg                                                                                                      <  2:1091746/238‑1 (MQ=255)

                                                                                                                                              ctttCGGGGTTGAACGCGGATTTCCTGAAAGG‑GACAGTGGGGGTGTTGGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGa                                                                                                     <  2:129724/237‑1 (MQ=255)

                                                                                                                                              ctgtCCGGTTTAAAAGCGGAATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGa                                                                                                     <  1:829951/236‑1 (MQ=255)

                                                                                                                                              ccTTCGGGTTTGAAGGCGAGTTTCCTAAAGGC‑GACAGATGAGCTGATTGCCGCCGTTCCAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGa                                                                                                     <  1:686/239‑1 (MQ=255)

                                                                                                                                              ccTTCCGTGTTGAAAGCGGCATTCCTGAAACC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACTCTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTGAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGa                                                                                                     <  1:93704/239‑1 (MQ=255)

                                                                                                                                              ccTTCCGGGTTGAAAGCGGAATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGa                                                                                                     <  1:306249/239‑1 (MQ=255)

                                                                                                                                               ctgcggtgTTGAACGAGGCATCCATTAAGGC‑GACAGATGTGTTGTTTCGCGCGGTTGCAGCCCCCTGGCGGG‑AAGATTTCCCGTTTGTGTGGCTA‑AATGGGG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGa                                                                                                     <  2:70152/231‑1 (MQ=255)

                                                                                                                                                 tCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTATGCGGCCG‑CAAGGAG‑ACTTCCACG‑CCGGGAATAGTCTCTTGCGCGATGGTCCCA‑CGTTTTTTGATCTTGGTGATGCTCGGACTGGTCCAGCCGTTCA‑GGGTTTGTGGGGG‑TTGCGCAATTGCGATAAAACCGAGCACCggggggagaggggaaa                                                                                                  >  2:607320/1‑225 (MQ=255)

                                                                                                                                                   cGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGGTTTCACCCGTCCGCTGCTC‑AATGGGG‑AATGCCACG‑CCGGGGAAATTTTCTGGCCGGATTGTCCAA‑TGTTTGTTTATCTGGATGATGCACGTAATTGGCCCGCCGGTCT‑GGGTTTTTGGGTGTTTGCTC‑ATGGCGATAAAGACGCGCAGGGGGAGCAACAGGGAAAta                                                                                                >  2:6305/1‑239 (MQ=255)

                                                                                                                                                          aaaGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCa                                                                                                                                                                                 <  1:154353/153‑1 (MQ=255)

                                                                                                                                                          aaaGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACC‑CCGGGAATATTTTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTTGATGATGCACGTAATGGTCCAGCCGTTCA‑GGGTTTTTGGATG‑TTGCTCAACGGGGGTAAAACCGAGCAGCGGATGCAACTGGAAACTattattta                                                                                         >  1:2573/1‑237 (MQ=255)

                                                                                                                                                          aaaGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAAATTTCCCCCCTTCGCGGGTC‑CAAGGGA‑AATGCCACC‑CCGGGAATATTTCCTGGGCGGACGGGCCAACTGTTTTTTGCTC‑GGCGGGTGCACGAAATGGGCCAGCCGGTCG‑GGGATTGGGGGGT‑TTGTTCAAAGGGGCTAAAACCCAGCCGCGGAGGCAAATGGAAAAAATTATTGa                                                                                         >  2:831286/1‑239 (MQ=255)

                                                                                                                                                          aaaGCCGCTTTCTGGAAAGA‑GACAGGTGAGCTGTTGCCCGCGGTTACAGCACCCTGGCGGG‑AAGATTTCCCCGTTCGGCGGCTA‑CATGGAG‑GCTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGa                                                                                         <  2:1085401/239‑1 (MQ=255)

                                                                                                                                                                   ttCCGGAAGGG‑GACAGGTGGGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCCCCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATga                                                                                <  1:211421/239‑1 (MQ=255)

                                                                                                                                                                                            aTTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGc                                                        <  1:15743/239‑1 (MQ=255)

                                                                                                                                                                                                      ttACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGa                                              <  1:185596/239‑1 (MQ=255)

                                                                                                                                                                                                                 tGGCGGG‑AAGATTTCACCGTTCTGCGTCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGc                                                                                      <  1:921675/188‑1 (MQ=255)

                                                                                                                                                                                                                            aTTTCCCCGTTCGGCGGCTC‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGa                                              <  1:42284/218‑1 (MQ=255)

                                                                                                                                                                                                                                   cGTTTTGGGGCTA‑CATGGGG‑GTGGCCACG‑CCGGGAATTTTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGtt                  <  2:829308/239‑1 (MQ=255)

                                                                                                                                                                                                                                   cGTTCTGCGGCTA‑CATGTAG‑CCTGCCACG‑CCGGGAATATTCTGTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCCCGTAAGTGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGtt                  <  1:948163/239‑1 (MQ=255)

                                                                                                                                                                                                                                   cGTTCTGCGGCTA‑CATGGGG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGtt                  <  1:466430/239‑1 (MQ=255)

                                                                                                                                                                                                                                   cGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGtt                  <  1:605695/239‑1 (MQ=255)

                                                                                                                                                                                                                                      tCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGACGAATTTAGCAAGTTCGACACACCTCAAATCGGACTGTTTTAAcccattttcttttaacaaacc                >  2:42284/1‑219 (MQ=255)

                                                                                                                                                                                                                                       cTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCCGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGt              >  1:563373/1‑239 (MQ=255)

                                                                                                                                                                                                                                          cGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGATTATGAAGAATTTAGCGAGTTCGAACCCCCTGAAATCGGAATGATTGAAACTTTACCCCCGCTGCGTTTGGtttt           >  2:1152/1‑238 (MQ=255)

                                                                                                                                                                                                                                                    ggAG‑ACTGCCACG‑CCGGGAATTTTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGc  <  1:445251/239‑1 (MQ=255)

                                                                                                                                                                                                                                                    ggAG‑ACTGCCACG‑CCGGGAATATTCTCGGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGc  <  1:85173/239‑1 (MQ=255)

                                                                                                                                                                                                                                                    ggAG‑ACGGCCACG‑CCGGGAATATTCTCTGGCTCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGc  <  1:28870/239‑1 (MQ=255)

AGATGGAAGCGGTTGGGCGTTATTTAGGGC‑GTATGCA‑CCAGACGGGGCGCAAACAGCTTTTTAT‑CCATCGCCCGACCATCGGTTTGAACGAATATCTC‑ATTGAGCCACGCAAGCTGTTTGA‑GGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGC‑GACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGG‑AAGATTTCACCGTTCTGCGGCTA‑CATGGAG‑ACTGCCACG‑CCGGGAATATTCTCTGGCGCGATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCA‑GGATTTGTGGATG‑TTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGC  >  NC_000913/4042775‑4043249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A