Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I0 R1 140 172.9 3477528 97.9% 3404499 226.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,342,036 A→C 16.3% D42A (GAC→GCC)  melA → alpha‑galactosidase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,342,0360AC16.3% 347.7 / 12.0 144D42A (GAC→GCC) melAalpha‑galactosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (74/38);  new base C (3/28);  total (77/67)
Fisher's exact test for biased strand distribution p-value = 1.71e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

ACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGC  >  NC_000913/4341799‑4342265
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       
aCTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGa                                                                                                                                                                                                                                         >  2:1626896/1‑238 (MQ=255)
aCTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGa                                                                                                                                                                                                                                         >  2:694320/1‑238 (MQ=255)
    ctgctTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGAcccc                                                                                                                                                                                                                                     >  2:654648/1‑238 (MQ=255)
     ttctTCGCGCAGTATTTGGTTTTAT‑GGAATTTCCAACCCGGAAGGCGGGAGTTTTCTTGAAGTTTCCCCGGCAATGATAAAGTTATTCACGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTGGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGTTGAAACCGGCGCATA‑TTGCCCTGATGGACATTGACCCCAc                                                                                                                                                                                                                                   <  1:1511773/237‑1 (MQ=255)
      gctTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCAc                                                                                                                                                                                                                                   >  1:1481088/1‑238 (MQ=255)
               gggATCTGAGTTTATGGGAATGCTCAACTGGGAAGCGGGAGGTTTTC‑TGCAGATTCGCCTGCCATGAGGAATTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCCCCCGCCTGGaa                                                                                                                                                                                                                         <  1:553318/238‑1 (MQ=255)
                 gATCTGAGTTTATGGGAATGCTCAACCTGGACGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAATATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGCTGATGTGTTCTATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAaga                                                                                                                                                                                                                       >  2:985000/1‑239 (MQ=255)
                  aTCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAaga                                                                                                                                                                                                                       <  2:1608776/238‑1 (MQ=255)
                  aTCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAaga                                                                                                                                                                                                                       <  2:960976/238‑1 (MQ=255)
                  aTCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAaga                                                                                                                                                                                                                       <  2:1125685/238‑1 (MQ=255)
                  aTCTGAGTTTATGGGAATGAGCAACGTGGAAGCCGGAGGTTTTC‑TGCAGATTGGCCTGCCATGATGAAGTTATTCAAGAAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTAATTTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTTATGTGTTCCATGGTGAGGCGTTGAAAACGGCGAATA‑TTGCCATGATGGACATTGACCCCACCCGCCTGGAaga                                                                                                                                                                                                                       <  2:181001/238‑1 (MQ=255)
                           tATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTACAAATATTCTTGGTGATGTGTTCCATCGCGCGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATAtt                                                                                                                                                                                                             >  1:2921/1‑239 (MQ=255)
                           tATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATAtt                                                                                                                                                                                                             >  2:1019878/1‑239 (MQ=255)
                           tATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATAtt                                                                                                                                                                                                             >  2:316650/1‑239 (MQ=255)
                           tATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATAtt                                                                                                                                                                                                             >  2:1483536/1‑239 (MQ=255)
                             tGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATCCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATtgt                                                                                                                                                                                                           <  2:231685/239‑1 (MQ=255)
                              gggAATGCTAACCCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTCCATTTATCGGCGCTGGTTCGAGGATTTTCGTTAAAAATATCCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATtgtg                                                                                                                                                                                                          <  2:565249/239‑1 (MQ=255)
                               ggAATGCTCAACCTGGAAGCGGGAGGTTTTC‑TGCAGATTCGCCAGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATtgtg                                                                                                                                                                                                          <  1:745166/238‑1 (MQ=255)
                                 aaTGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTgtggt                                                                                                                                                                                                        >  1:1080444/1‑238 (MQ=255)
                                   tGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCAc                                                                                                                                                                                                                                   <  2:1455768/209‑1 (MQ=255)
                                   tGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCAc                                                                                                                                                                                                                                   >  1:1455768/1‑209 (MQ=255)
                                   tGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCg                                                                                                                                                                                                     >  2:1434697/1‑239 (MQ=255)
                                               gaGCAGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGCAAACAGCGCATA‑TTGCCCTGCTTGAGATTGACCCCACCAGACTGGAAGAGTCGCTACTTGTGGTGCGTAAGCTGATGGa                                                                                                                                                                                         >  1:174586/2‑239 (MQ=255)
                                                    ggAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTca                                                                                                                                                                                     >  1:95280/1‑238 (MQ=255)
                                                     gAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTcagc                                                                                                                                                                                   >  2:904764/1‑239 (MQ=255)
                                                            tC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGcc                                                                                                                                                                            >  2:1018684/1‑239 (MQ=255)
                                                                              cATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCCGGGGCCAGCGGCAGCATCACCt                                                                                                                                                            >  1:1231733/1‑238 (MQ=255)
                                                                                     aaGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACAcc                                                                                                                                                    <  2:1308259/239‑1 (MQ=255)
                                                                                     aaGTTATTCAAGAAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTAAAAAATATTCTTGGTGATGTGTTCCATCGCGGGGCGCTGAAAACGGGGCATA‑TTGCCCTGATGGACATTGCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACAcc                                                                                                                                                    <  1:560682/239‑1 (MQ=255)
                                                                                               aagagacggggAGATCTCCTATATGGCTGAACCAAAATTTACTTTTATGGGCGGTGGTTCACCGATTTGCGTAAAAAATATTTTTGGTGATGTGTCCCACCGCGAGGCGCTGAAACGGCCGAATA‑TTGCCCTGGTGGACATTGACCCCCCCCGCCTGGAAGAGTCGCATATTGGGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACagaaag                                                                                                                                          <  1:1536988/230‑1 (MQ=255)
                                                                                                gcaagcCAGGGGATCTGCATGATTTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGAt                                                                                                                                                                                            <  1:1344655/188‑1 (MQ=255)
                                                                                                gcaagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGAt                                                                                                                                                                                            >  2:1344655/1‑188 (MQ=255)
                                                                                                gcaagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACagaaaga                                                                                                                                         >  2:1245753/1‑239 (MQ=255)
                                                                                                gcaagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACagaaaga                                                                                                                                         >  2:370851/1‑239 (MQ=255)
                                                                                                gcaagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACagaaaga                                                                                                                                         >  1:1266480/1‑239 (MQ=255)
                                                                                                gcaagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCAAGCGGCAAAATCACCTGCCACACCCAAcagacaga                                                                                                                                         >  1:454016/1‑239 (MQ=255)
                                                                                                  aagcCGGGAGATCTGCAGGATGTCTACACCAAAAATTACATTTAGCGGCGCTGGTTGGAGGATTTTGTTTAAAAATATTCTTGGTGATGGGTTCAATCGCGGGGCGGTGAAAAGGGCGAATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACagaaaga                                                                                                                                         <  1:326113/237‑1 (MQ=255)
                                                                                                  aagcAAGGAGATCTGCATGATGTCTGCACCAAAATTTACATTTATCGGCGCTGGTTCGCGGATTTTCGTAAAAAATATTCTTGGTGATGTGTTCCATGGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGAAATTGCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACagaaaga                                                                                                                                         <  2:1454325/237‑1 (MQ=255)
                                                                                                    gcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAaagaag                                                                                                                                       >  2:865243/1‑237 (MQ=255)
                                                                                                    gcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCCCCCGCCTGGAAGAGTc                                                                                                                                                                                                                    <  2:1674110/160‑1 (MQ=255)
                                                                                                    gcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTc                                                                                                                                                                                                                    >  1:1674110/1‑160 (MQ=255)
                                                                                                              cTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTc                                                                                                                                                                                                                    <  1:696659/150‑1 (MQ=255)
                                                                                                              cTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGAt                                                                                                                            >  2:249083/1‑238 (MQ=255)
                                                                                                              cTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGAt                                                                                                                            >  2:1704737/1‑238 (MQ=255)
                                                                                                               tGCATGATTTCTGCCCCAAAAATTACATTTATCGGGGCTGGTTGGACGATTTTCGTAAAAAATATTCTTGGTGAGGTGTCCCATCGCGGGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCGGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGc                                                                                                                          <  1:1434697/239‑1 (MQ=255)
                                                                                                               tGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAACTATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGCCAAAATCATCTGCCACACCCAACAGAAAGAAGCCTTCGAGGAtt                                                                                                                           >  1:105571/1‑237 (MQ=255)
                                                                                                               tGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATg                                                                                                                           >  2:828281/1‑238 (MQ=255)
                                                                                                               tGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATg                                                                                                                           >  2:843260/1‑238 (MQ=255)
                                                                                                                   tgatgTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTaga                                                                                                                                >  2:1536988/1‑229 (MQ=255)
                                                                                                                   tgatgTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAt                                                                                                                      >  1:1555072/1‑239 (MQ=255)
                                                                                                                      tgTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAtttt                                                                                                                   >  2:1683850/1‑239 (MQ=255)
                                                                                                                      tgTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAtttt                                                                                                                   >  2:298991/1‑239 (MQ=255)
                                                                                                                         cTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGCTTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGTTGGACATTCCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCCGGGGCCAGCGGCAAAATCACCTGCCCCACCCAACAGAAAGCAACCTTAGTGGATGCCCATTTTGta                                                                                                                >  1:824691/1‑238 (MQ=255)
                                                                                                                         cTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTc                                                                                                                >  1:1253933/1‑239 (MQ=255)
                                                                                                                           gCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgt                                                                                                              >  1:1132673/1‑239 (MQ=255)
                                                                                                                             aCCAAAAATTCCATTTAGCGGGGTTGGTTCGACGATTTTCGTAAAAAATTTTTTTGGGGAGTTGTCCCATCGCGGGGCGCGAAAAACGGCGCATT‑TTGCCCGGAGGAACTTTGCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgtgg                                                                                                            <  1:513559/239‑1 (MQ=255)
                                                                                                                                  aaaTTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCa                                                                                                       >  2:637217/1‑239 (MQ=255)
                                                                                                                                         aTTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCCGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTCGAGGCTGCCGGTTTTGTACTGGTGGCATTTCAGa                                                                                                >  2:469860/1‑239 (MQ=255)
                                                                                                                                         aTTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGa                                                                                                >  2:785551/1‑239 (MQ=255)
                                                                                                                                             accggccttGGTGCGACTTTTTCCTTTAAAAATTTTCTGGGTTAGGGTTCCCACCGGGAGGCGCTAAAAACGGCCCTTA‑TTGCCTTGAGGAAAATTGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGTATTTCAGA‑TTgg                                                                                           <  1:638445/231‑1 (MQ=255)
                                                                                                                                             aTCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTg                                                                                            >  2:1409337/1‑238 (MQ=255)
                                                                                                                                             aTCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTg                                                                                            >  2:73160/1‑238 (MQ=255)
                                                                                                                                             aTCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTCTGGCGTGGTGGCATTTCAGC‑TTg                                                                                            >  1:1175973/1‑238 (MQ=255)
                                                                                                                                             aTCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGACTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGCAAGAAGCCTTAGAGGCTGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTg                                                                                            >  2:1097740/1‑238 (MQ=255)
                                                                                                                                                ggCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCgg                                                                                        >  2:24521/1‑239 (MQ=255)
                                                                                                                                                  cgcTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTCGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGC‑TTGGCggct                                                                                      >  1:661711/1‑237 (MQ=255)
                                                                                                                                                     tgtttCGACGATTTTCGTTAAAAATATTCTTGGTGATGTATTCCATGGCAAGGGGCTGAAAACGGGGCATA‑TTGCCCTGATGGACATTGCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑At                                                                                   <  2:949276/235‑1 (MQ=255)
                                                                                                                                                     ggtttCGACGATTTTCTTAAAAAATTTTGTTGGTGATGTGTCCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATGGACCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑At                                                                                   <  2:858264/235‑1 (MQ=255)
                                                                                                                                                       gTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGaa                                                                                >  2:86926/1‑239 (MQ=255)
                                                                                                                                                              gaTTTTCGTTAAAAATTTTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCa                                                                         <  2:519111/239‑1 (MQ=255)
                                                                                                                                                                     ttaaaaatTATTCTTGGGGATGTGTCCCACCGGGAGGCGCTGAAACCGGCGAATA‑TTGCCCGGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGGGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGAc                                                                  <  2:1592287/236‑1 (MQ=255)
                                                                                                                                                                     gTTAAAAATATTTTTGGTGATGTGTTCGCTCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGAGGGACTTGGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGAc                                                                  <  1:386422/239‑1 (MQ=255)
                                                                                                                                                                       tAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGAGGCATTTCAGA‑TTGGCGGTTCATAAACCTCCAACGGTgtcg                                                                 >  1:1446820/1‑236 (MQ=255)
                                                                                                                                                                               ttCTTGGTGATGTTTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAg                                                        <  2:1627627/239‑1 (MQ=255)
                                                                                                                                                                                   tGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAAACTTGCACGGTGACTGATTTCGAgg                                                       >  2:387955/1‑236 (MQ=255)
                                                                                                                                                                                   gggggatggtttCCATCGCGAGGCCCGAAAAAGGGCGCTTT‑TTGCCCTGAGGGACATGGCCCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCt                                                    <  1:1007954/229‑1 (MQ=255)
                                                                                                                                                                                    ggTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑GTGTAACCTGCGCGGTGAccgtcttcatgg                                                       >  2:568362/1‑224 (MQ=255)
                                                                                                                                                                                       gATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCCCCCGCCTGGAaga                                                                                                                                                                                                                       <  1:809901/74‑1 (MQ=255)
                                                                                                                                                                                       gATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAaga                                                                                                                                                                                                                       >  2:809901/1‑74 (MQ=255)
                                                                                                                                                                                        aTGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGAT‑ATGAACATTGCACAGAGGCGGTTTTCTAGGACTGTaaa                                               >  1:1017155/1‑238 (MQ=255)
                                                                                                                                                                                             ttCCAGCGGGAGGCCCGGAAAACGG‑GCAAATTTGCCGTGAGGGGAATTGCCCCCCCCCGCCTGGAAGAGTCCCATATTGTTGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCa                                          <  1:1680289/239‑1 (MQ=255)
                                                                                                                                                                                             tccctcggggggggcttGAAACCGGCGCTTA‑TTGCCTTGATGGACATTGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCa                                          <  2:1080444/223‑1 (MQ=255)
                                                                                                                                                                                             tcccACCGCGAGGCGCTGAAAAGGGGGCATT‑TTCCCCTGTGGAACATTGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCa                                          <  2:785030/237‑1 (MQ=255)
                                                                                                                                                                                             taccccggcgAGGCGCGGAAAACGGCGCATA‑TTGCCCTGAGGGAAATGGACCCCACCCGCCTGGAAGAGTCGCATAGTGTGGTGCGTAAGCTGATGGATCCAGCAGGGGCCGGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCa                                          <  1:654648/232‑1 (MQ=255)
                                                                                                                                                                                              tCCACCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGAACATTGCCCCCACCCGCCGGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCa                                          <  1:1612328/238‑1 (MQ=255)
                                                                                                                                                                                              tCCACCGCGAGGCGCAGAAACCGGCGATTA‑TTGCCCTGATGAACATTGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCa                                          <  1:86926/238‑1 (MQ=255)
                                                                                                                                                                                              cccATCGCGAGGCGCTGAAACCGGCGCATA‑TTGCCTTGAGGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCa                                          <  1:1483536/237‑1 (MQ=255)
                                                                                                                                                                                                 tgccggcggCGCTCAAACCGGAGCTAT‑TTGCCCTTGTGCACATTCACCCCACCTGCCTGGAAGGGTCGCATTTTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGACAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGt                                      <  1:1283414/232‑1 (MQ=255)
                                                                                                                                                                                                  tccggtggCGCTGAAAGCGGCGAATA‑TTCCCCTGATGGAAATTGACCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTc                                     <  1:1704737/233‑1 (MQ=255)
                                                                                                                                                                                                       aGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑CTGTACCTTGCACGGTGGCTGGTTTTGAGGttgttaaagggtccagtctggg                                 >  2:1264148/1‑217 (MQ=255)
                                                                                                                                                                                                                    ggCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGT‑GCATTTTAAATTTGGGGGGT‑TTGAACCTTGCCCGGGGGCTGGTTTCGGGGTCTGAAAGGGGACGGGTCGTGACAAAACCAttccgc                   >  1:1387819/1‑235 (MQ=255)
                                                                                                                                                                                                                      cgcTTA‑TTGCCCTGATGGACATGCCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGa                  <  1:785551/238‑1 (MQ=255)
                                                                                                                                                                                                                      cccATA‑TTGCCCTGATGGACTTGGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGa                  <  2:735603/236‑1 (MQ=255)
                                                                                                                                                                                                                       tcctttttcccttgttgcactttGTCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCTCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGa                  <  1:1018684/217‑1 (MQ=255)
                                                                                                                                                                                                                       cgaattttGCCCTGAGGGACAGTGCCCCCCCCCGCCTGGAAGAGCCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGa                  <  1:576123/232‑1 (MQ=255)
                                                                                                                                                                                                                       cgaatagggcctggATGGACAGTCACCCCGCCCGCCTGGAAGAGTCGCATAGTGTGGTGCGTAAGCGGAGGGAGGCAGCAGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGa                  <  1:913747/225‑1 (MQ=255)
                                                                                                                                                                                                                                cccggagggccttgcaCCCCGCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTgggg        <  1:838257/224‑1 (MQ=255)
                                                                                                                                                                                                                                    gtgggACATGGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCgg    <  1:370851/236‑1 (MQ=255)
                                                                                                                                                                                                                                     aTGGAAATTGCCCCCCCCCGCCTGGAAGAGTCTCATATTGTGGTGCGTAAGCTGTTGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCggg   <  2:1333831/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gACATTGCCCCCCCCCGCCTGGAAGAGGCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGc  <  2:1324575/237‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       
ACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTC‑TGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCATCGCGAGGCGCTGAAAACGGCGCATA‑TTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGA‑TTGGCGGTT‑ATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGC  >  NC_000913/4341799‑4342265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A