Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,029,689 T→G 11.2% T428P (ACT→CCT)  fadB ← fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,029,6890TG11.2% 293.2 / 3.0 120T428P (ACT→CCT) fadBfused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (50/52);  new base G (12/3);  total (65/55)
Fisher's exact test for biased strand distribution p-value = 2.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 121 total aligned reads displayed.

ACGGGAACAGCACGCGGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTA  >  NC_000913/4029455‑4029925
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
aCGGGAACAGCACGCGGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑Ta                                                                                                                                                                                                                                            <  2:209563/239‑1 (MQ=255)
      acggCACGGGTTTAACAACGTAACGGGGGCCGTCGTTACGCACAATCGGGGGCCTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGGTTTGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGc                                                                                                                                                                                                                                       <  1:1139342/235‑1 (MQ=255)
       cAGCACGCGGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGTTTGAAGTGT‑TAGACGccc                                                                                                                                                                                                                                     >  2:1139535/1‑238 (MQ=255)
        agaccgcgGTTAACAAAGAGGCCGGGGCAGTCGTTAACCACATTCGGTGTCTTGC‑CCATCTTGCCCGCCCAGGCGACAACTTTCGCGATGGTGTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAg                                                                                                                                                                                                                                    <  1:803493/235‑1 (MQ=255)
         gCACGCGGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAgtgctgtctctttta                                                                                                                                                                                                                                    >  2:32033/1‑227 (MQ=255)
         gCACGCGGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGGGT‑TAGACGCCAGc                                                                                                                                                                                                                                   >  2:1014445/1‑239 (MQ=255)
          cACGCGGTTAACAAAGGAGCCGGGGCAGGAGTTAACCACATTCGGCGGTTTGC‑CCATTTTGCTCGCCCAGGCGATAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCa                                                                                                                                                                                                                                  <  1:702275/239‑1 (MQ=255)
           aCGCGGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGcc                                                                                                                                                                                                                                      <  2:1097337/234‑1 (MQ=255)
           aCGCGGTTAAAAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCa                                                                                                                                                                                                                                  <  2:339934/238‑1 (MQ=255)
            cgcgGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGGGT‑TAGACGCCAGCACg                                                                                                                                                                                                                                >  2:666982/1‑239 (MQ=255)
                       aagaagCCGGGGCAGTTGTTAACCACAAGCGGCCTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCg                                                                                                                                                                                                                    <  2:1268766/239‑1 (MQ=255)
                       aagaagCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTCAAGAAGTGCATCCCGC‑CGAAGTTTTCCGGGCGTTCCAGCGCGTGGGCCAGTTCGCTGATGGGAATGGTTGAAGGGT‑TAAAGCCCAGCCGGGAA‑TCCggggg                                                                                                                                                                                                                    >  1:1237664/1‑237 (MQ=255)
                       aagaagCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAATTGCCTCCCGC‑AGACGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGGTGATAGGAATGGTTGAATGGT‑TGAAGCCCAGCACGGTC‑GCCGGGCg                                                                                                                                                                                                                    >  1:1057069/1‑239 (MQ=255)
                         gaagCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGt                                                                                                                                                                                                                   >  2:270718/1‑238 (MQ=255)
                         gaagCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGGGT‑TAGACGCCAGCCCGGGA‑TCCtctgt                                                                                                                                                                                                                    >  2:803493/1‑233 (MQ=255)
                                   gcttgcttAACCCCAAGCGG‑GTCTTTCGCCATTGTGCCTGCCCATGCTACACCTTTGGGGATGGGTTCGTGGGAGCTTTTCTCGCCGGGAATAATTTCTTCCAGCGGCAGTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTGTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGTAATGGGTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGtt                                                                                                                                                                                                         <  1:1014445/232‑1 (MQ=255)
                                   catgtggtAACCGCAATCGGCGTCTTGC‑CCTTCTTGCCCGCCCAGGCGACAACTTTCGGGATGGTTGCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGtt                                                                                                                                                                                                         <  2:1035681/231‑1 (MQ=255)
                                   aaGTCGTTACCCCCAATCGGCGGTGTCC‑CCATCTTCCTCTCCCCGGCCACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTTCCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCCTCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGtt                                                                                                                                                                                                         <  1:116408/237‑1 (MQ=255)
                                      tCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACg                                                                                                                                                                                                                                <  2:99763/213‑1 (MQ=255)
                                       cggtgaccgtgttCGGCGTCTTGC‑CCTTGTTGCTCGCCCAGGCGACAACTTTCGGGATGGTTTCGGCGGAGCTGTTCGCGCCGCGAATAATGTCTACCAACGGCATGCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGTCGTTCCAGCGCGTTGGCCGGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGt                                                                                                                                                                                                     <  1:188504/226‑1 (MQ=255)
                                          tcaccccagtggtcttCTTGC‑CCATCTTGGTGGCCCAGGGGACAACTTTCGCGAGGGTTTCGTGGGAGCTTTCCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCt                                                                                                                                                                                                 <  1:628194/224‑1 (MQ=255)
                                          taaaccaaaTCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCCACAACCTTCGAGATGGTTTCGTCGGAGCTTTTCACGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGGAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCCCGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCt                                                                                                                                                                                                 <  2:189452/232‑1 (MQ=255)
                                           aaCCACAATCGGCGTATTGC‑CCATCTTGCTCGGCCAGGCGACAACTTTCGCGATGGTTTCGCCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAAGGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑GGAAGTTCTCCTAGAGTTCCAGCGCGTTGGCCAGTCGTCTGTGAGGGATGTTTGAA‑TGTGTAGACGTCAGCACGGTG‑TCAGGGCGCTCGTTGGGTTCTGTTTCt                                                                                                                                                                                                 >  1:729749/1‑238 (MQ=255)
                                                caATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAg                                                                                                                                                                                            >  2:273620/1‑238 (MQ=255)
                                                caATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAg                                                                                                                                                                                            >  2:527549/1‑238 (MQ=255)
                                                caATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGACCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCCTCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCCCGCATAGGAATGGTTGACGTGT‑TAG‑CTCCCACACCGCACTCCTGGCGCACTTTTTGTTGGATTTCtcacaga                                                                                                                                                                                           >  1:953771/1‑233 (MQ=255)
                                                caATCGGCGTCTTGC‑CAATATTGCTCGACAAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCAGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGGTACAGACGTGCATCCCGC‑AGCAGATTTCCGGGCGTTCCAGCGCGTTGGCGCGTTCGCTGATAGGACTGGTCGAAGTGT‑TCGCCTCCAGCGCGGTA‑TCCTCGCGGCCTTTTTTTTCGGTTTCGGCCAGt                                                                                                                                                                                           >  1:80093/1‑239 (MQ=255)
                                                caATCGGCGTCTTGC‑ACATCTTGCTCGCCCAGGCGACAACTTTCGCGCTGGTTTCGCCGCACCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGACGTGCATCCCCC‑AGATGATTTCCGAGGGTTCCAGCGCGTTATCCCGTTCGCTGGTTGGACTGGTTGTACTGT‑TAGCCGCCACCCCGGTA‑TCCCAGCTCACTTTTTTTTCGGGTACTGccgga                                                                                                                                                                                           >  1:171178/1‑236 (MQ=255)
                                                 caTCGGCGTTTTGC‑CCCTTTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTa                                                                                                                                                                                          <  2:1127328/238‑1 (MQ=255)
                                                  aTCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCCGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTTTGCCAGTaa                                                                                                                                                                                         >  1:763626/1‑238 (MQ=255)
                                                       cGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGGGT‑TATACGCCAGCCCGGTA‑TCCTGGCGTACTTTTTGTTCGGGTTCTGCCAGTACGGCtt                                                                                                                                                                                    >  2:98665/1‑239 (MQ=255)
                                                        gTCTTGC‑CCATCTTGCTCGACCATGAGACCACTTTCGCGACGGTTTCGCCGGAGCTCTTCTCGCCGCGCATAATTTCTACCAACGGCATTCGGTGGACCGGGTTACTGAAGTGCATCCCGC‑AGAAGTTTGCCGCGCGTTTCAGCCGCTTGGCCGGTTTGCTCGAAGTAATGATTGAGGTGC‑TTGAAGCCACAACGGGG‑TACGTGCGTACTCTTTGTACGGTTTCTG‑CAGTTCGGGtttg                                                                                                                                                                                  >  1:839319/1‑238 (MQ=255)
                                                          cTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTAGCTGTAAGGAATGGTTGAAGGGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTTTGCCAGAAGGGCttttt                                                                                                                                                                                 >  1:303791/1‑239 (MQ=255)
                                                          cTTGC‑CCATCTGGCTCGCCGAGGCGACAACTTGCGCGATGGTTTCGACGGAGCTTTTCGCGCCGCGAATAATTGCTTCCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCtttt                                                                                                                                                                                  <  2:382294/238‑1 (MQ=255)
                                                           ttGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCtttttt                                                                                                                                                                                >  2:413502/1‑239 (MQ=255)
                                                              c‑ccATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGACGTGT‑TAGAAGCCAGCACGGCA‑TCCTGGCGTAATTTTTTTTCGGTTTCTCCCGGAACGGCTTTTTTc                                                                                                                                                                               >  1:1025457/1‑237 (MQ=255)
                                                                   acgttcccGCCCAGGCGAAAACTTTGCGGGTGGGTTGTGCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTc                                                                                                                                                                         <  1:611935/232‑1 (MQ=255)
                                                                      tGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCggg                                                                                                                                                                      <  2:303791/239‑1 (MQ=255)
                                                                                ggCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCCCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTGCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTcaacaa                                                                                                                                                            <  2:161301/239‑1 (MQ=255)
                                                                                 gCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTcaa                                                                                                                                                               >  2:611935/1‑235 (MQ=255)
                                                                                    aCAACTTTCGCGGTGGTTCCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCAAGCACGGTA‑TCCGGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGc                                                                                                                                                        <  2:1038026/239‑1 (MQ=255)
                                                                                      aagtctccagATGGTGTGGTCGTAGCTCTTCTCGCCTCGAATCATTTCTACCAACGGCATTCGGTGGACCGTGTTAAAGAAGTGAATCCCGC‑AGCAGTTTTCCGGGAGTACCAGCCCGTTGGCCAGTTCGCTGATAGGTGTGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGTCGTCCTTTTTGTTCGGTTTTTCCCAGTACGGCCTTTTTCACTTTCGGGTTTTcaaaacaccgtc                                                                                                                                                      >  1:261051/10‑230 (MQ=255)
                                                                                       aCTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTc                                                                                                                                                     <  2:158217/239‑1 (MQ=255)
                                                                                        cTTTCGCGGTGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCt                                                                                                                                                    <  2:979256/239‑1 (MQ=255)
                                                                                          ttCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGGGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTAc                                                                                                                                                  <  2:362527/239‑1 (MQ=255)
                                                                                          ttCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTAc                                                                                                                                                  <  2:249396/239‑1 (MQ=255)
                                                                                          ttCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGGGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTCCGGCTTTTTTCCCTTTCGGGTTTTCACCAACCGCTTTTa                                                                                                                                                   >  2:546950/1‑238 (MQ=255)
                                                                                                                  ttCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTAAAAAACCGCTTCCACCACAATA‑TACAAGCGGTCAAAccc                                                                                                                         >  1:869171/1‑239 (MQ=255)
                                                                                                                      cgccgcGAATTATTTCTACCAACGGCCGTGGGTGGACCGGGTTAAAGAAGTGCATCCCGA‑AGAAGTTTTCCGGGCGTTCCAGAGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCCCGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCg                                                                                                                     <  1:1039888/239‑1 (MQ=255)
                                                                                                                      cgccgcAAATTATTTCTTCCAACGGCATTCGGTGGCCCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAAGGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCCCTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCg                                                                                                                     <  2:1071111/239‑1 (MQ=255)
                                                                                                                       gccgcGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGAGT‑TAGACGCCAGCACGGGA‑TCCTAGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTTAACAACCCCTTCTACCCAAAAA‑TCACCGCggcacaatcgcgagt                                                                                                                    >  1:966650/1‑226 (MQ=255)
                                                                                                                          cggAATTATTTTCACCACCGGCATTGGGGGGCCCGGGTTAAAGAAGGGC‑TACCGCGAAAAGTTTCCCGGGCGTTACGGCGCGTTGGCCAGTTCGCTGATGGGTATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCGGCCAGTACGGCTTTTTTCCCTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGt                                                                                                                 <  1:666982/237‑1 (MQ=255)
                                                                                                                            gAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTc                                                                                                                <  2:1265820/238‑1 (MQ=255)
                                                                                                                                 atTTCTCCCAAGGGCTTTCGGTGGCCCGGGTTAAAGAAGTGCATCCCGC‑AGAGTTTTTCGGGGGTTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGCCGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGt                                                                                                          <  1:527549/239‑1 (MQ=255)
                                                                                                                                 atTTCTCCCAACGGCCTTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGt                                                                                                          <  2:720423/239‑1 (MQ=255)
                                                                                                                                 atTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGt                                                                                                          <  2:1025457/239‑1 (MQ=255)
                                                                                                                                   ttCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCCCAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTg                                                                                                        >  2:710783/1‑239 (MQ=255)
                                                                                                                                    tCTACCAACGGTCTTGGGGGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGTGTTCCAGCGGGTTGGCCAGTTGGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTgg                                                                                                       <  2:511704/239‑1 (MQ=255)
                                                                                                                                       aCCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCCCGGTA‑TCCTGGCGAACTTTTTGTTCGGTTTCTGCACGTACGGCTTTTTTCACTTTCGGGTTTTCAAAACCCGCTTCACACCCAATA‑CCCACGCGTTCCAATCCGCGGTAGTCCACAGTTGGGTg                                                                                                    >  1:511704/1‑239 (MQ=255)
                                                                                                                                               ctttCGGTGGACCGGGTGAAAGAAGGGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGt                                                                                                     <  2:1158933/228‑1 (MQ=255)
                                                                                                                                                       ggACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCAc                                                                                     >  2:1274622/1‑238 (MQ=255)
                                                                                                                                                         aCCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAGGTGT‑TAGACGCCAGACCGTAA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCCGTACGGCTTTTTTCACTTTCGGGCTTTCACAAACCGCCTCTACACCAATA‑TCCCAGCGGTCAAATCCGGCGTATCAGAGCGTTGGGGGGATTGGGgagcgggctct                                                                                  >  1:1158933/1‑231 (MQ=255)
                                                                                                                                                         aCCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGGGGATTGTGGGGATCAcccc                                                                                  >  2:640868/1‑239 (MQ=255)
                                                                                                                                                            gggTTAAAAAAGTGAATCCCGA‑AGAAGTTTTCCGGGGGTTCCAGGGCTTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACgccagc                                                                               <  2:1241622/239‑1 (MQ=255)
                                                                                                                                                             ggTTAAAGGAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTGCCAGCGCGTTGGCCGGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACgccagc                                                                               <  2:467769/238‑1 (MQ=255)
                                                                                                                                                             ggTTAAAGAAGTGCATCCCGC‑AGTAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCCTTTTTCACTTTCTGGTTTTTAACAATCGCTCCTCCACCAATA‑TCCACACGGTCAAATCCGGCCTGGTCCAGCCTTGGGGGGTTTGTCGGGGTCACgacagcc                                                                              >  1:896772/1‑239 (MQ=255)
                                                                                                                                                             ggTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACACTA‑TCACCGCGGTCAAATCCGCCGTAGTCGAGCGTTGGGtctgtctcttataaccatccccg                                                                               >  2:1107960/1‑216 (MQ=255)
                                                                                                                                                             ggTTAAAGAAGTGCATCCCAC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGGATGGTTGAAGGGT‑TAGGCGCCAGCTCGGTA‑ACCTGGCGTACGTTTTGTTCGGTTTCTGCCAGTACGGCATTTTTGCCTTTCGGGTTTTTAAAAACACCTACTACAAAAAAT‑TCCACACGGTAAAACCCCACGATGTCGAGCGTTGGGGGGCttagagcggtaaccccagaa                                                                              >  1:1205427/1‑221 (MQ=255)
                                                                                                                                                              gTTAAAGAAGGGCCTCCCAG‑CGAATGTTTCCGGGGGTGCCCGCGCGTTGGCCAGTGCGCTGGTAGGAAGGGTTGAAGGTT‑TAGGCCCCAGCAGTGTA‑TCCTGGCGTACTTTTTGTCCGGTTTCTGCCAGTACGGCTTTTGTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCCCGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACgccagc                                                                               <  1:1049238/237‑1 (MQ=255)
                                                                                                                                                                       gTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATG‑TCCACGCGGTGAAATCCGGCGTAGACGAGCGTTGGGTGGTTTGGGACGAACACgccaacagagtcaggc                                                                     >  1:334326/1‑226 (MQ=255)
                                                                                                                                                                       gTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGa                                                                     >  2:1097169/1‑238 (MQ=255)
                                                                                                                                                                       gTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCAGCAAGTACGGCTTTTTTCCCTTTCGGGTTTTCAACAACGGCTTCTACCCCAATATTCCAC‑AGGTCAAATCCGGCCTAACCGAGCGTTGGGCGGATTGTGGGGAACACCCCAACCAGgttacgac                                                                    >  1:430472/1‑234 (MQ=255)
                                                                                                                                                                        tgaaTCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATCGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTTGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGCTTTCACAAACCGCTTCTACCACAACA‑TACCCTGGGTCAAGACAGGCGTAGTCAACAGTTGGTGGGATTGTGGAGATCACACAAGCCAGTTGCataac                                                                   >  1:598398/4‑235 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGCTAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTAAGGCTTTTTTCACCTTCGGGTTTTCTACAACCGCTTCTACCACAATA‑TCCACGCGGGCAAATCCGGCGTATTCCACCGTTGGGGGGATTTGGGAGAACCCCCCACACAGTTTCAgcct                                                                   >  1:193877/1‑236 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAg                                                                                                                                                                                                                                    <  1:1169771/80‑1 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAg                                                                                                                                                                                                                                    >  2:1169771/1‑80 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCCCACTA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGGGGATTGTGGGGATCACGCCATCCGGTTTCAGAcc                                                                   >  2:242610/1‑239 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGGGATCACGCCCGCCAGTTTCAGAcc                                                                   >  2:612652/1‑239 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGa                                                                     >  2:1049238/1‑237 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCACACCGTTTCAGccc                                                                   >  1:1164852/1‑239 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTCCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGGGATCACGCCAGCCAGTTTcggccc                                                                   >  2:77075/1‑234 (MQ=255)
                                                                                                                                                                        tGCATCCCGC‑AGAAGATTTCCGTGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGGATGGTTGAAGTGT‑TAGACGCCAGACCGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTAACTTTCGGGTTTTCAACAAACGCGTCTACCACAATC‑TCCACGCGGTCAAATCCTGCGTTGTAGATGGTTGGGTGGATTGTGGGGAGCAGGCCACACAGTTTTAAAcc                                                                   >  1:837114/1‑239 (MQ=255)
                                                                                                                                                                               cGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCTCTTCTACCACAATA‑TCCCCGCGGTCCAATCCGGCGTATTCCAGCGTTGGGTGGTTTGTGGAGAACACCCACACCAGtttacaaccacgca                                                              >  1:608950/1‑226 (MQ=255)
                                                                                                                                                                                        ttttCCGGGGTTTCCAGGGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCcgcg                                                    <  2:646963/239‑1 (MQ=255)
                                                                                                                                                                                               ggCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGACCTTGCCGCGCTCaa                                               >  2:1113681/1‑237 (MQ=255)
                                                                                                                                                                                               ggCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACCCGTTCAAATCCGGCGTAGTCGACCGTTGGGGGGAATTTGGAGGTCACCCCACCCGGTTTCCAACCATCgtaatttccgccctccacc                                             >  1:1201870/1‑221 (MQ=255)
                                                                                                                                                                                                  gTTCCAGCGCGTTGGCCAGTTCGCTGATAGTAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGGAGTCGAGCGTTGGGAGGATTGTGAAGATCCCGCCAGCCGGCTTATGACCATCCACATTGTCGCGCCCaaagcgt                                          >  1:416774/1‑234 (MQ=255)
                                                                                                                                                                                                          ggcttggcattttttgtGATAGGGTTGTGTGTAGTTT‑TAGACGCCAGCACGGTT‑CCCTGTGTTTGTTTTTGTACGGGTTTGGCCAGTACGGCTTTTTTCCCTTCGGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTcag                                  <  1:558524/223‑1 (MQ=255)
                                                                                                                                                                                                              tGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGt                              >  2:832337/1‑239 (MQ=255)
                                                                                                                                                                                                                  cAGTTCGCTGATAGGAATGGTTGAAGGTT‑TAGGCGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTcg                          <  2:181153/239‑1 (MQ=255)
                                                                                                                                                                                                                     ttCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCCCGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCCCGCGGTCAAATCCGGCGTAGTCCAGAGTTTGGTGGGTTGTGGAGCACACGCCATCCCGTTTCAGACCACCGATCGTGCCGTCCCCAAGTTCCTTTTTCACCAGTTTcccg                        >  1:708683/1‑238 (MQ=255)
                                                                                                                                                                                                                            aTAGGAATGTTTGAAGTTT‑TAGACGCCAGCCGGGTA‑GCCGGGCTTTCTTTTTGTTCTGTTTCTGCCAGTACGGCTTTTTTCCCTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGa                                                                                                  <  2:536306/157‑1 (MQ=255)
                                                                                                                                                                                                                               ggAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCTGGGCTCAACGGGCTTGTTCAGCAGTTTCGCGTCTTCGGTCAt             >  1:1212165/1‑239 (MQ=255)
                                                                                                                                                                                                                                   tGGTTGAAGTGT‑GAGCCGCCAGCCCGGTT‑TCCTGGCGTTCTTTTTGTGCGGTTTCTGCCAGTACCGCTTTTTTCACTTGCGGGTTTTCAACAGCCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCg         <  2:171178/239‑1 (MQ=255)
                                                                                                                                                                                                                                    ggTTGAAGTGT‑TAACCGCCAGCCCGGTT‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACATCCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGa        <  2:896772/239‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTGAAGTGT‑TAGACCCCAGCACTGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCg         <  2:326942/237‑1 (MQ=255)
                                                                                                                                                                                                                                      ttGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAgg      >  2:214787/1‑239 (MQ=255)
                                                                                                                                                                                                                                      ttGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGAGGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCCACGACCTTGCCGCGCTCAAGCTGATTGTGCAGCAGTTTCGCGGCTTCCGTCTTGCCGCgg      >  1:974008/1‑239 (MQ=255)
                                                                                                                                                                                                                                          aGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGCGGGTTa  >  2:157899/1‑239 (MQ=255)
                                                                                                                                                                                                                                          aGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCCCGGCTTCGGTCATGCCGAGGGTTa  >  2:1189506/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
ACGGGAACAGCACGCGGTTAACAAAGAAGCCGGGGCAGTCGTTAACCACAATCGGCGTCTTGC‑CCATCTTGCTCGCCCAGGCGACAACTTTCGCGATGGTTTCGTCGGAGCTTTTCTCGCCGCGAATAATTTCTACCAACGGCATTCGGTGGACCGGGTTAAAGAAGTGCATCCCGC‑AGAAGTTTTCCGGGCGTTCCAGCGCGTTGGCCAGTTCGCTGATAGGAATGGTTGAAGTGT‑TAGACGCCAGCACGGTA‑TCCTGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATA‑TCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTA  >  NC_000913/4029455‑4029925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A