Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F27 I0 R1 120 139.6 2835800 98.5% 2793263 226.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,364,905 T→G 14.5% V225G (GTG→GGG)  puuB → gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,364,9050TG14.5% 299.3 / 2.8 109V225G (GTG→GGG) puuBgamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (20/68);  new base G (19/2);  total (39/70)
Fisher's exact test for biased strand distribution p-value = 1.12e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTC  >  NC_000913/1364670‑1365140
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
tACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAAATTTCTTCCGCGCCGCACATCCCCCACCCCCCCCACCCCGGTGCGCGCACTGCCACAGGCCAGGTgc                                                                                                                                                                                                                                            >  2:678714/1‑237 (MQ=255)
 aCGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGC‑GATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACg                                                                                                                                                                                                                                          >  1:221499/1‑238 (MQ=255)
     caaaaaaaggCTGGAATGGCTGAACGCAAAAGCCATTCGCCGTGAAGTAGCCAGCGATCGCGATACCGGTGCGCTGCTGGATCACAGCGGTGGGCAAATTCATCCGCTAAACCTGGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                                                                                                                                                                                                                                       <  2:910557/229‑1 (MQ=255)
     cAATATACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATCCCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGCCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACATCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                                                                                                                                                                                                                                       <  1:521847/238‑1 (MQ=255)
     cAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                                                                                                                                                                                                                                       <  1:511713/238‑1 (MQ=255)
     cAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                                                                                                                                                                                                                                       <  1:1366155/238‑1 (MQ=255)
     cAAAAAACAGCTGGAATTGCGGGACGCCAACGCCATTCGCCGTGAAGTGGCCAGCGATCGCTATACCGGTGCGCTGCGGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                                                                                                                                                                                                                                       <  2:549800/238‑1 (MQ=255)
        tcaaCAGCGAGAGTTGCTGCGCGGTAGCGCCAAGCCCCGTGATGGACCCAGCGATCGCTACGCCGCTCCGCTGCTGGAACACCGCGTTGGGCTTAGTCAGCCCCTAATCCTTGCCGTTGACGGAGGTGTCGCTCTCCGCCTCAACGGCTGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACGCCACGCCAGGCGTTGTGCGAACTGCTAGAGGTCAGGTGACGGCGaa                                                                                                                                                                                                                                     <  2:770338/235‑1 (MQ=255)
        tAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCTGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCCGGACGCCGATCCAGCACACCACCCCCGCCGGTGTGCGAACTGCCAAAGGTCAGGGGACGGCGAAgg                                                                                                                                                                                                                                   >  1:414827/1‑238 (MQ=255)
               cTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGGTGTCCGAACTGCCACAAGTCAGGGGACGGGGAAGTATGTGaa                                                                                                                                                                                                                            >  1:1369423/1‑238 (MQ=255)
               cTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCGGGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGGGACGGCGAAGGAttggat                                                                                                                                                                                                                            >  1:1241771/1‑234 (MQ=255)
                     ttGCTGGACGCCACCGCCATTCGCCGTGAAGTCGCCAGCAACCGCTATCCCGGTGCGCTGCTGGATCACAGGGGTGGGCATATTCATCCGCTAACCCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGc                                                                                                                                                                                                                      <  2:927728/239‑1 (MQ=255)
                         tGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCgg                                                                                                                                                                                                                   <  2:1036683/238‑1 (MQ=255)
                          ggACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGa                                                                                                                                                                                                                 <  1:926391/239‑1 (MQ=255)
                             cgccaacgccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAAt                                                                                                                                                                                                               <  1:829737/238‑1 (MQ=255)
                             cgccaacgccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTCTTTGCCGTGTCGCAGATCCCGCACACCACCCCAGCCGCTGTGCGAACCGCCAACAGGCCGGGGACGGCGCAGTCTTGGATTTTCGCCgcggaa                                                                                                                                                                                                               >  2:638340/1‑233 (MQ=255)
                                     ccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATg                                                                                                                                                                                                              <  1:657644/231‑1 (MQ=255)
                                       aTTCGCCGTGAAGTAGCCAGCGATCGCTATCCCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTggg                                                                                                                                                                                                    <  1:1246673/239‑1 (MQ=255)
                                        ttCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTTTTCCGTGGCGCCAATCCAGCACAACACGCCAGCCGGTGTGCGGACTGCCCACGGGCAGGGGGCGGCGGAGGATGTGGTTGTTCCCGGGAATGCGTAAGTGGGc                                                                                                                                                                                                   >  2:1181551/1‑239 (MQ=255)
                                        ttCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGGGGATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCCGCCGTTGTGCGGACTGCCAAAGGGCAGGGGACGGCGAAGTATGTGGTTGTCGCCGGGGATGCGTAGTTGGGc                                                                                                                                                                                                   >  2:916730/1‑239 (MQ=255)
                                               tGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTTCCGTGACGCCGATCCAGCCCACCACGCCCGCCGTTGTGCGGACTGCCCAAAGGCAGGGGGCGGCGGAGAATGTGGTTGTCGCCGGGGCTGCGGATATGGGcccgtc                                                                                                                                                                                              >  2:657644/1‑232 (MQ=255)
                                                              tCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGGGACGCAGATCCAGCACACCACGCCAGCCGGTGTGCGGACTGCCAAAGGTCAGGTGACGGCGAAGGATGTGATTGTCGCCGGGGATGCGTATCTGGGCGATAAAGTAGAGCCGGCACTg                                                                                                                                                                              >  1:1152442/1‑238 (MQ=255)
                                                                gCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATGGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTggg                                                                                                                                                                                                    <  1:1322595/214‑1 (MQ=255)
                                                                       cGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgca                                                                                                                                                                    <  1:1105486/239‑1 (MQ=255)
                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCCGCACACCACGCCAGCCGTTGTGCGGACTGCCCAAGGTCACGGGCCCGCGGAGTATGTTGTTGTTGCCGGGAATGCGTATCTGGGGCGTAAAAGAGCTCCGTAACTCGCGAAAcacca                                                                                                                                                                   >  2:72617/1‑234 (MQ=255)
                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcagc                                                                                                                                                                  >  1:438940/1‑239 (MQ=255)
                                                                          tGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCGTGACGCCGATCCAGCCCACCACCCCAGCCGCTGTGCGAACTGCCCAAGGTCAGGGGGCGGCGAAGTATGGGCTTGTCGCCGGGGACGCGGATCTGGGGGATAaagaagctgtctcttttacacctctgacg                                                                                                                                                                  >  2:1322595/1‑215 (MQ=255)
                                                                          tGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCGGGACGCAGATCCAGCACACCACGCCAGCCGGTGTGCGAACTGCCCAAGGGCAGGTGACGGCGAAGAATGTGAGTGTCGCCGGGGATGCGTATCTGGGCGCTAAAGTAGAGGCGGGAATGGCGGAACgcagga                                                                                                                                                                 >  2:395319/1‑237 (MQ=255)
                                                                          tGCGCGGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcagc                                                                                                                                                                  <  2:1383407/238‑1 (MQ=255)
                                                                          ggcgcTGCTGGATCACAGCGGTGGCCATATTCAGCCGCTAAACCTTGCCATGGGCGAAGCGTACGCCACCCGCCTCAACGGCGGGCGCTTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGTAACTGGCGAAACgcagc                                                                                                                                                                  <  1:313010/237‑1 (MQ=255)
                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCCGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGGCAGGGGCCGGCGAAGAATGTGATTGTCGCCGGGGATGCGGATCTGGGCGATAAAGTCGAGCCCGGACTGGCGAAACGCAGCAt                                                                                                                                                                >  2:134660/1‑238 (MQ=255)
                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGCGTATGAACTTTCTTCCGGGACGCAGATCCAGCACACCACGCCAGCCGGTGTGCGAACTGCCAAAGGGCAGGTGACGGGGGAGGATGTGATTGTCGCCGGGGATGCGTATCTGGGCGATAAAGTAGAGCCGGGACCGGGGGAACgccagct                                                                                                                                                                >  2:1000333/1‑233 (MQ=255)
                                                                             gcttctGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAGTGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATg                                                                                                                                                               <  1:408408/238‑1 (MQ=255)
                                                                              ctgctgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGGCGCAGATCCAGCACACCACGCCAGCCGGTGTGCGAACTGCCAAAGGTCAGGGGACGGCGAAGGATGTGATTGTCGCCGGGAATGCGGATCTGGGCGATAAAGGAGAGCCGGAACTGGGGAAACGCAGCATGc                                                                                                                                                              >  2:1349444/1‑238 (MQ=255)
                                                                              ctgctgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAAATTTTTGCCGTGACGCAGATCCCGCACACCACGCCAGCCGTTGTGCGCACTGCCAAAGGTCAGGGGACGGCGAAGGATGTGATTGTCGCCGGGGATGCGTATCTGGGCGATAAAGAAGAGCCCGAACTGGCGAAACCCAGCACGcc                                                                                                                                                             >  2:1330455/1‑239 (MQ=255)
                                                                                  tgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTg                                                                                                                                                          <  1:984297/238‑1 (MQ=255)
                                                                                  tgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTg                                                                                                                                                          <  1:507142/238‑1 (MQ=255)
                                                                                  tgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTg                                                                                                                                                          <  2:580485/238‑1 (MQ=255)
                                                                                  tgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTg                                                                                                                                                          <  1:199732/238‑1 (MQ=255)
                                                                                  tgGATCACAGCGGTGGGCATATGCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTg                                                                                                                                                          <  2:83074/238‑1 (MQ=255)
                                                                                     aTCACGGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCgg                                                                                                                                                       <  1:649997/238‑1 (MQ=255)
                                                                                      tCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCa                                                                                                                                                     <  1:248220/239‑1 (MQ=255)
                                                                                        acaGCGGTGGGCAGTTTCATCCGCTAAACCGTGCCATGGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACg                                                                                                                                                   <  2:222689/239‑1 (MQ=255)
                                                                                           gCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGGGGGGTTTTGGCATTTCTGCCGCGGCCCCGCTCCAACACACCACGCCAGCCGGTGTGCGGACTGCCAAACGGCAGGGGACGGCGCAGGATATGATTGTCCCCGGGGAAGCGTATGTGGGGGATAAAGTAGGACCGGAACCAGCGCAAccgcgcaatccgggggggacgaag                                                                                                                                                >  2:910227/1‑216 (MQ=255)
                                                                                           gCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGGGTATGAAATTTCTGCCGTGACGCAGATCCAGCACACCCCGCCCGCCGTTGGGCGAACTGCCAAAAGGCAGGTGACGGCGAAGGAAGGGATTGTCGCCGGGGATGCGGATCTGGGGGGGAAAGTAGAGCGCGGACTGGCGAAAACCAGCAAGCCGTGGGGGGCGCAg                                                                                                                                                >  2:14493/1‑239 (MQ=255)
                                                                                           gCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGGGGATGAACTTTCTGGCCTGACCCAGATCCCGCACACCACCCCCGCCGCTTTGCGAACTGCCCCAAGGCAGGTGGCGGCGAAGTATGTGCTTTTCGCCGGGGAAGCGGCTCTGGGCGGTTAAGTAGAGCCCGGACCGGCGCAACCCCGCATGCCGGGCgggccccag                                                                                                                                                >  2:916217/1‑232 (MQ=255)
                                                                                            gggTGGGCTTATCCACCCGCTAAACCTGCCAATGGGCAAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAg                                                                                                                                                <  2:73444/237‑1 (MQ=255)
                                                                                                 ggCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCCGATCCCACACAACACGCCAGCCGTTGTGCGGACTGCCAAAGGTCAGGGGAAGGCGAAGGATGTGGTTGTCGCCGGGGATGCGTATCTGGGGGATAAAGTAGAGCCGGGACTGGGGGAACACAGCATGCCCGGCGGCCCGCAGGGGATc                                                                                                                                          >  2:941864/1‑239 (MQ=255)
                                                                                                 ggCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCCGCCGTTGTGCGAACTGCCAAAGGGCAGGGGGCGGCGAAGGATGTGATTGTCGCCGGGAATGCGGATCTGGGCGATAAAGTAGAGCCGGGACTGGCGGAACGCAGCATGCCGCGCGGCACCCCGGGGAtg                                                                                                                                          >  2:85217/1‑238 (MQ=255)
                                                                                                          acccGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGaa                                                                                                                                 <  1:1181551/237‑1 (MQ=255)
                                                                                                             cccTAAACCTTGCAGTGGGCGAGGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACgg                                                                                                                              <  2:673502/237‑1 (MQ=255)
                                                                                                                  aaCCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGGGACGCAGATCCAGCCCACCCCGCCAGCCGGTGTGCGAACTGCCCAAGGGCAGGGGGCGGCGAAGGATGTGATTGTCGCCGGGGATGCGTATCTGGGCGGGAAAGTAGAGCCGGGACTGGCGAAAAGCAGCATGCCGTGCGGCAAGCCGGTGATAACCACCGAACGGCT‑GTc                                                                                                                        >  2:183806/1‑239 (MQ=255)
                                                                                                                    ccTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACCCCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCg                                                                                                                                                                                    <  1:79966/178‑1 (MQ=255)
                                                                                                                    ccTGGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCgg                                                                                                                      <  1:1253488/239‑1 (MQ=255)
                                                                                                                       tGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCCGGACGCGCATCCAGCACACCACCCCAGGCGTTGGTCGGACTGCCCAAGGCCCGGGGACGGCGGAGTCTGAGATTGTCGGCCGGTATGCGGATCTTGGCGGTAAAGTAGAGCCGGAACTGGCCAAACACAGCAAGCCCGGCGGGACGCAGGTGATCCGCACCCGACGGCTGGTCGGag                                                                                                                    >  2:493863/1‑238 (MQ=255)
                                                                                                                             tGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAg                                                                                                             <  1:85217/239‑1 (MQ=255)
                                                                                                                                 gAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAGCTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGGATGCGTAGCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCg                                                                                                         <  1:58218/239‑1 (MQ=255)
                                                                                                                                         cGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGGGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGGCGGCGAAGGATGTGATTGTCGCCGGGGATGCGGATCTGGGCGGTAAAGTAGAGCCGGAACAGGCGGAACGCAGCATGCCGGGCGGCACGCCGGGGATCACCACCCGACGGCT‑GTCGGGAGATTTAGGCCCGTCGCTgg                                                                                                 >  2:186071/1‑238 (MQ=255)
                                                                                                                                            caaccGCGTCAACGGCGGGCGCGTTTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGATCTGCCAAAGGTCAGGTGACGGCGAAGTAGGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATcc                                                                                              <  2:1368636/236‑1 (MQ=255)
                                                                                                                                            cATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATcc                                                                                              <  1:14493/239‑1 (MQ=255)
                                                                                                                                                      aaCGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAacta                                                                                    <  1:678714/239‑1 (MQ=255)
                                                                                                                                                      aaCGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAacta                                                                                    <  1:24824/239‑1 (MQ=255)
                                                                                                                                                      aaCGGCGGGCGCGTGGATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAacta                                                                                    <  1:910227/239‑1 (MQ=255)
                                                                                                                                                        cggcggGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAactact                                                                                  <  2:810515/239‑1 (MQ=255)
                                                                                                                                                         ggcgggggCGTGTAGGAACTTTCTGCCGGGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAacta                                                                                    <  2:257113/236‑1 (MQ=255)
                                                                                                                                                                gcgTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGaa                                                                          <  1:256900/239‑1 (MQ=255)
                                                                                                                                                                gcgTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGGTGTGCGGACTGCCAAAGGGCAGGGGACGGCGAAGTATGTGATTGTCGCCGGGGATGCGTATCTGGGCGATAAAGTAGAGCCGGGACTGGCGAAACGCAGCATGCCGTGCGGGACGCAGGGGATCACCCCCGAACGGCC‑GTCGGGAGAGTTAGCCCGTTCGCTGATCCCGCAAAACTAACGTGTggga                                                                          >  2:374935/1‑237 (MQ=255)
                                                                                                                                                                gcgTGTATGAACTTTCGGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGaa                                                                          <  2:623920/239‑1 (MQ=255)
                                                                                                                                                                  gtgtATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGa                                                                        <  1:1198191/239‑1 (MQ=255)
                                                                                                                                                                      aTGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGTCGGTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGt                                                                    <  2:839301/239‑1 (MQ=255)
                                                                                                                                                                      aTGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGt                                                                    <  1:916730/239‑1 (MQ=255)
                                                                                                                                                                        gAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTaa                                                                  <  2:684731/239‑1 (MQ=255)
                                                                                                                                                                           cTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTa                                                               <  1:72617/239‑1 (MQ=255)
                                                                                                                                                                              tCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGCAGATTTAGCCCGTTCGCTGATCCCGAACAACTACTGTGCGGGAGACTGTAACTa                                                               >  2:376969/1‑236 (MQ=255)
                                                                                                                                                                              tCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGaaaaa                                                                                       <  2:1165838/212‑1 (MQ=255)
                                                                                                                                                                              tCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGaaaaa                                                                                       >  1:1165838/1‑212 (MQ=255)
                                                                                                                                                                                tGCCGTGACGCAGATCCAGCACACCGCGCCAGCCGTTGTGCGAACTGGCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGc                                                          <  2:357046/239‑1 (MQ=255)
                                                                                                                                                                                tGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAATTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAGACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTAGCTGc                                                          <  1:961412/239‑1 (MQ=255)
                                                                                                                                                                                tGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGc                                                          <  2:1335769/239‑1 (MQ=255)
                                                                                                                                                                                tGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGc                                                          <  2:438940/239‑1 (MQ=255)
                                                                                                                                                                                 gCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCt                                                         <  1:1049568/239‑1 (MQ=255)
                                                                                                                                                                                      gACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGAt                                                     <  1:435341/238‑1 (MQ=255)
                                                                                                                                                                                              tCCAGCACACCCCGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATtactac                                               <  2:308298/236‑1 (MQ=255)
                                                                                                                                                                                                    acacCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTAcc                                      >  1:483444/1‑239 (MQ=255)
                                                                                                                                                                                                    acacCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTAcc                                      <  1:1124130/239‑1 (MQ=255)
                                                                                                                                                                                                       ccacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATc                                                             >  1:208873/1‑213 (MQ=255)
                                                                                                                                                                                                       ccacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATc                                                             <  2:208873/213‑1 (MQ=255)
                                                                                                                                                                                                        cacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTAccgccg                                  >  2:274411/1‑239 (MQ=255)
                                                                                                                                                                                                        cacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTAccgcc                                   >  2:752052/1‑238 (MQ=255)
                                                                                                                                                                                                              ggCCGTTGTGCGAAGTGCAAAAGGTCAGGGGACGGCGAAGTTTGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAAcc                            <  1:587784/238‑1 (MQ=255)
                                                                                                                                                                                                                ccGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCg                           >  1:279862/1‑238 (MQ=255)
                                                                                                                                                                                                                ccGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCg                           >  1:1388553/1‑238 (MQ=255)
                                                                                                                                                                                                                ccGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCg                           >  1:458438/1‑238 (MQ=255)
                                                                                                                                                                                                                         gAACTGCGAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTa                  <  1:575090/238‑1 (MQ=255)
                                                                                                                                                                                                                           aCTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTAcgg               <  1:374935/239‑1 (MQ=255)
                                                                                                                                                                                                                             tGCCAAAGGTCAGGGGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTAcggcg             <  1:916217/239‑1 (MQ=255)
                                                                                                                                                                                                                             tGCCAAAGGGCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTAcggcg             <  1:1000333/239‑1 (MQ=255)
                                                                                                                                                                                                                                cAAAGGTCGGGTGACGGCAAAGTATGTGAGTGTCGCCGGAAATGCGTATCTGGGCGAAAAAGTAGAGCCGGAACGGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTCCCGTCTTACCGCCGACAACCGCCTGCTGTAcggcggcg          <  2:510158/239‑1 (MQ=255)
                                                                                                                                                                                                                                  aatgTCAGGTGCGGGCGAAGTATGTGATTCCCGCCGGGAATCCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCCCCGAACGGCT‑GTCGGAAGATTTAGCCCTTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGAAACTAGCTGCTTGATTCCTACCGTCTTACCGCCGACAACCGCCTGCTGTACggcggcggc        <  2:1099494/236‑1 (MQ=255)
                                                                                                                                                                                                                                         ggTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGATCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTggcc  >  1:1231944/1‑236 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
TACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCT‑GTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTC  >  NC_000913/1364670‑1365140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A