Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F9 I0 R1 71 67.1 1514203 90.7% 1373382 220.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,015,350 T→C 13.1% intergenic (+37/+3) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,015,3500TC13.1% 179.4 / 3.2 84intergenic (+37/+3)metE/ysgA5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand):  ref base T (28/43);  new base C (3/9);  total (31/53)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGC  >  NC_000913/4015122‑4015576
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   
tATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGGGCAGGCGGCGCAGAACTTGCGTCGGGGGGAAAATCCAA‑ACCGGGTGGTAATACCCCCCGGGCTTTtct                                                                                                                                                                                                                                     >  1:626761/1‑229 (MQ=255)
 aTGACTTTCACTCGCTAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCGGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACGTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTtct                                                                                                                                                                                                                                     <  1:138068/228‑1 (MQ=255)
    aCATTCACTCGCCAAACGTACCGAGCGAGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCAtt                                                                                                                                                                                                                                 <  1:625194/229‑1 (MQ=255)
        tCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCCCGGCTGGCCAGAAACCCGCGCGCGACTGGCCAACCTGGGGCAGGCGGCGCAGAACTTGCGTCGGGGGGAAAAACCCA‑ACCGGGTGGTAATAACCCCCGGGCTTTTCTCAttcaa                                                                                                                                                                                                                              >  2:698345/1‑225 (MQ=255)
           cgccccAAGCGTACCGAGCGGGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAACCGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGa                                                                                                                                                                                                                          <  1:25570/225‑1 (MQ=255)
            tCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGGGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGGCTTTTCTCCTTACAGCGAc                                                                                                                                                                                                                         >  1:49380/1‑229 (MQ=255)
              gCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGAACTGGCGAACAGGGTGCAGGCGGCGCCGAACTTGCGCCGGGGGGAAAACCCAACACCGGG‑GGGAAAACCCCCCGGCCTTTTTCCCTTACaaggcatt                                                                                                                                                                                                                       >  2:369869/1‑222 (MQ=255)
                     gttccgggggTGGAATGGATTGAAGCCTTGATGAAAAAAGCGGTAAAACGCATTCCGGCAGGGCGCCTGGTGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCcca                                                                                                                                                                                                                <  2:414611/220‑1 (MQ=255)
                          gggggttgAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATa                                                                                                                                                                                                           <  1:151885/222‑1 (MQ=255)
                          gAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAACACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTCCCGCGGGGGTAAAAGCCCA‑ACCGGGGGGGAAAACCCCCAGGTCTTTTCTCCTTACAGCGACCCGTTCccaccca                                                                                                                                                                                                            >  2:482112/1‑226 (MQ=255)
                          gAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGACCTTGCGTCGGGGGGAAAATCCAA‑ACCGTGTGGTAATACCACCCGGGCTTTTTTCCTTACACCGACTTCTTCCCACCAt                                                                                                                                                                                                            >  1:149973/1‑228 (MQ=255)
                          gAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGGAAAATCCAA‑ACCGGGGGGTGAGCCCCCCCGGCCTTTTCTCCtttccggcgcctcttccccccaa                                                                                                                                                                                                            >  2:413408/1‑207 (MQ=255)
                          gAGCGTGGAAGGGATTGAAGCCTGGGTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATa                                                                                                                                                                                                           <  1:281593/229‑1 (MQ=255)
                               tGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCCCAGAACTTGCGCCGGGGGTAAAATCCAA‑ACCGGGTGGGAATAACCCCCGGCCTTTTTTCATTCCAGCGCCCTCTTCCCCCCCTACCGc                                                                                                                                                                                                       >  2:623902/1‑228 (MQ=255)
                               tGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGGGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCt                                                                                                                                                                                                      <  1:554075/229‑1 (MQ=255)
                                 gAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCCGGGGCAAAATCCAA‑ACCCGCGGGGAAACCCACCCGGGCTTTTTCCCTTACACCGCCTTCCTCCCACCATACCGCCTa                                                                                                                                                                                                    >  2:243145/1‑229 (MQ=255)
                                   tgtgATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGGGCGCCTGGGGGTCAACCCGGACTGGGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTaaa                                                                                                                                                                                                  <  2:626760/226‑1 (MQ=255)
                                   agggATTGAAGCCTGGCTGAAGAAAGCGGCAAAACGCATTCCGGGAGAGCGCCTGTGGGTCAACCCGGACTGGGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTaaa                                                                                                                                                                                                  <  2:285010/227‑1 (MQ=255)
                                       attggaagctggcggaagaaAGCGGCAAAACGCAGTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGGCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCTGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCa                                                                                                                                                                                               <  2:207527/214‑1 (MQ=255)
                                       aTTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCa                                                                                                                                                                                               >  1:26910/1‑228 (MQ=255)
                                         tgaacgcTTGCTGAAGAAAGCGGCAAAACAGATTCCGGGAGAGCGCCTTTGGGTCAACCCGGACGGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGTGCGGCACTGGCGAACATGGGGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAAGACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATTCTGCTTAAACCAtt                                                                                                                                                                                             <  2:472591/222‑1 (MQ=255)
                                           aaGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGGGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGGGGGAATACCACCCGGGCTTTTCTCATTAAAAAGACTTCTTCCCACCAAAATCCTTAAACCCTTcc                                                                                                                                                                                           >  2:685354/1‑228 (MQ=255)
                                            aGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGGCTTTTCTACTTACAGCAACTTCTTCCCACCATACTCCTTACACCATTCCac                                                                                                                                                                                         >  1:339466/1‑228 (MQ=255)
                                                ttGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCCGCGGGGGTAAACTCCAA‑ACCGGGCGGTAATCCCACCCGGTCTTTTCCCCTTACACCGAATTCCTCCCACCCCACTGCATAAAACATTTCCG‑CAt                                                                                                                                                                                     >  2:696585/1‑228 (MQ=255)
                                                ttGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAAAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGGGGTAATCCCACCCGGTCTTTTCTCATTTCAGCGACTTCTTCCCCCCATACCCCTTAAACCATTCCAG‑Caa                                                                                                                                                                                     >  2:738266/1‑227 (MQ=255)
                                                         aaaGCGGCAAAACGCATTCCGGCAGAGCGCCTGGGGGTCAACCCGGACTGGGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTgccag                                                                                                                                                                           <  1:55090/229‑1 (MQ=255)
                                                           cgccgCAAACCCCATGCCGGAAGAGCGCCTGTGGGTCAACCCGGGCGGTGGCCGGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACTCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTgccagcc                                                                                                                                                                         <  2:322009/225‑1 (MQ=255)
                                                           aGCGGCAAAACGCCATCCGGCAGGGCGCCGGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCTAACATGGTGCAGGCGGCCCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTgccagcc                                                                                                                                                                         <  2:507153/229‑1 (MQ=255)
                                                                cAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCCGAACTTGCGTCGGGGGTAAGATCCAA‑ACCCGGGGGTAATACCACCCGGGCTTTTTTCTTTAAAACGGATTCTTACCCCCATAACTCTTAAACCCTTCCAGTCACAC‑CTCCCAGCCAactt                                                                                                                                                                    >  2:693340/1‑229 (MQ=255)
                                                                cAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGAAAAATCCAA‑ACCGGGTGGTAATACCACCCGGCCTTTTCTCATTACAGCGACTTCTTCCCACCCTACTGCTTAAACCATTCCAG‑CATACCCTGCCAGCCAtctt                                                                                                                                                                    >  1:356861/1‑229 (MQ=255)
                                                                    aCGCATGCCGGGAGGGCGCCTTTGGGTCCACCCGGACTGTGGCCTGAAACCGCGGGGCGGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTg                                                                                                                                                                 <  2:219660/228‑1 (MQ=255)
                                                                       cagtCCGGGAGGGCGCCTGTGGGTGAAGCCGGCCTGGGGCCCGAAAACGGGGGGCTGGCCAGAAACCCGCGCGGCACTGGCGAAAATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGa                                                                                                                                                             <  2:383190/226‑1 (MQ=255)
                                                                          tgcggcggGGCGCCTGGGGGTCAACCCGGACTGTGGGCCGAAAACGCGCGGCTGGCCAGAAACCCGGGGGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATg                                                                                                                                                           <  2:247256/226‑1 (MQ=255)
                                                                           ccGGCGGAGCGCCTGTGGGTCACCCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGATAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCg                                                                                                                                                         <  2:228273/229‑1 (MQ=255)
                                                                            cgggagggggcCTGTGGGGCCCCCCGGACTGGGGCCTGAAAACCGGCGGGCGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCgg                                                                                                                                                        <  2:615350/220‑1 (MQ=255)
                                                                            cGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCg                                                                                                                                                         <  1:369869/228‑1 (MQ=255)
                                                                                    cgcCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTTGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGGGGTAATACCACCCGGTCTTTTCTCATTACAGGGCCTTCTTCCCACCATAATGCTTTAACCATTCCCG‑CATACGCTGCCAGCCCTCTCCTGACGATGCGGCATGATaa                                                                                                                                                >  2:744789/1‑228 (MQ=255)
                                                                                    cgcCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGGAAAATCCAA‑ACCGGGTGGGAATACCACCCGGTCTTTTCTCATTCAAGCGAATTCTTCCCACCATCATGCTTAAACCCTTCCAC‑CATAACCTCCCCGCCAGCTTCTGCAAAGCCGGCAtaaaag                                                                                                                                                >  2:395329/1‑224 (MQ=255)
                                                                                       cTGGGGGTCAACCCGGACGGTGGCCCGAAAACGCGCGGCTGGCCAAAAACCCGGGCGGCACTGGCGAACAGGGTGCAGGCGGCGCAGGACTTGCGTCGGGGGTAAAAGCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTTCCAGCCATCTTCTGCAGATGCGGCATGATAGCTc                                                                                                                                             <  2:577053/229‑1 (MQ=255)
                                                                                                 ccccGGACTGGGGCCTGAAACCGCGGGGGTGGCCAGAAACCCGCGCGGCACTGGCGAAAATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAAt                                                                                                                                   <  2:339466/228‑1 (MQ=255)
                                                                                                 aCCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGCAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTTTGCAGATGCGGCATGATAACTCGGGCGCTAAt                                                                                                                                   >  1:503130/1‑229 (MQ=255)
                                                                                                       aCTGTGGCCTGAAAACGCGCGGCTGTCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCCGGGGTAAAACCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTAAAGCGACCTCCTCCCACCTTACTGCCTAAACCATTCCCG‑CCAACGCTGCCTGCCACCCTCTGCAGATCCGCCACGAAAGCCCGTCCGCTAAtaccac                                                                                                                              >  2:132324/1‑223 (MQ=255)
                                                                                                        cgtgggCCTGATACCGGGCGGTGGGCCAGAAACCCGGTCGGCACTGGCGAACAGGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCACATGACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGt                                                                                                                             <  2:431134/224‑1 (MQ=255)
                                                                                                               cggAAAACGCGGGGCCGGCCAGAAACCCGGGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAatgca                                                                                                                     <  1:599063/227‑1 (MQ=255)
                                                                                                                       gcgcgGGTGGCTCGAAACCCGGGCGGGCCTGGCGGACAAGGTGCGGGGGGCGCAGAACTTGGCGCGGGGGTAAAAGCCAA‑ACCGGGTGGTAATACCCCCCGGTCTTTTCTCATTACAGCGCCTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCgg                                                                                                              <  2:567609/228‑1 (MQ=255)
                                                                                                                        cgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTgccag                                                                                                                                                                           <  2:195211/166‑1 (MQ=255)
                                                                                                                        cgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTgccag                                                                                                                                                                           >  1:195211/1‑166 (MQ=255)
                                                                                                                        cgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGCCGATAATCAGCGTTGAATGCATGCCCGGc                                                                                                             >  1:647517/1‑228 (MQ=255)
                                                                                                                         gcgGCTGGCCAAAAACCCGCGGGGCACTGGCGAACATGGGGCGGGCGCCGCAGACCTTGCGTTGGGGGTAAAATCCAA‑TCCGGGTGGGAATACCACCCGGGCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCg                                                                                                            <  2:618678/228‑1 (MQ=255)
                                                                                                                                 ccAAAACCCCGGGCGGCCCTGGCGAACATGGGGCAGGCGGAGCAGAACTTGCGTCGGGGGTAACATCCAA‑ACCGGGTGGTAATACCCCCCGGTCTTTTCTCATTACAGCGACTTCGTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGt                                                                                                     <  2:31839/227‑1 (MQ=255)
                                                                                                                                     aaaCCCGGGCGGCACTGGCGAACATGGTGAGGGCGGCGCAGAACTGGCCTCGGGGGTAAAATCCAA‑ACCTGGTTGTAATACCTCCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CAGTCGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGa                                                                                               <  1:744790/229‑1 (MQ=255)
                                                                                                                                          cgcgcgGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATTCGGCATGATAGCTCGGGCGATAATCACCGTTGAATGCATGCCCGGCGTCCCGGTACACggaaat                                                                                           >  1:624462/1‑223 (MQ=255)
                                                                                                                                                  tcTGGCCAAAATGGTGCATGCGGCTCAAAACTGGCGTCGGGGGTAAAATCCAA‑CCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGCCTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCttt                                                                                  <  2:49380/228‑1 (MQ=255)
                                                                                                                                                           cATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGCTAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTag                                                                          >  1:689774/1‑228 (MQ=255)
                                                                                                                                                                   aggctgtcccgcaactGCGTCGGGTGTAAAACCCAA‑ACCGGGTGGTAATAACACCCGGTCTTTTCTGATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCa                                                                 <  2:485485/214‑1 (MQ=255)
                                                                                                                                                                   aGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGc                                                                  >  1:602678/1‑228 (MQ=255)
                                                                                                                                                                   aGGCGGCCGAGAACTTGCGTCGGGGGTAAAATCCAA‑CCCGGGTGGTAATCCCCCCCGGTCTTTTCCCATTACAGCCACTTCCTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCa                                                                 <  2:26910/229‑1 (MQ=255)
                                                                                                                                                                    ggctggcgaGAACTTGCGTCGGGGATAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCa                                                                 <  1:685355/220‑1 (MQ=255)
                                                                                                                                                                         cgcAGAACTTGCCTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATTCCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGcc                                                            <  1:30966/228‑1 (MQ=255)
                                                                                                                                                                           cAGAACTTGCGTCTGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTgg                                                         <  1:395329/229‑1 (MQ=255)
                                                                                                                                                                                tttcgctgtggggGAAGACCCAA‑GCCGGGGGGGAATACCCCCCGGTCTTTTCCCATTTCGGGGCCTTTTTCCCGCCATCCTGCGTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCa                                                     <  2:356861/219‑1 (MQ=255)
                                                                                                                                                                                cTTGCTGCGGGGGTAAAATCCAA‑ACCGGGGGGTAATACCCCCCGGTCTTTTCCCAGTACGTCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCGTTCGCATTAGCAGCCCGCAGCGCCTGGCGCa                                                     <  2:640006/228‑1 (MQ=255)
                                                                                                                                                                                          ggttAAACTCCAA‑ACCGGGTAGGAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACg                                          <  1:698346/226‑1 (MQ=255)
                                                                                                                                                                                          ggttAAAATCCAA‑ACCGGGTGTTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTCCCCACCATACTGCTTAAACCATTCCAG‑CATCCGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACg                                          <  1:696586/226‑1 (MQ=255)
                                                                                                                                                                                          gggTAAAATCCAA‑ACCGGGTGGTAATTCCACCCGGTCTTTTCCCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACg                                          <  1:522326/229‑1 (MQ=255)
                                                                                                                                                                                               aaaTCCAA‑ACCGGGTGGTAATACCACCAGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCtctc                                     <  1:302699/229‑1 (MQ=255)
                                                                                                                                                                                                    cAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCg                                >  1:21833/1‑229 (MQ=255)
                                                                                                                                                                                                     ca‑aCCGGGTGGTAATCCCACCCGGTCTTTTCCCATTCCAGCGACTTCTTCCCCCCATACCGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCg                                <  2:624461/227‑1 (MQ=255)
                                                                                                                                                                                                     aa‑aCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCg                                <  1:568690/228‑1 (MQ=255)
                                                                                                                                                                                                     aa‑aCCGGGTGGTAATACCACCCGGTCTTTTCCCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCg                                <  1:630187/228‑1 (MQ=255)
                                                                                                                                                                                                      a‑aCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCTGGCGATAATCAGCGCTGAATGCATGCCCGGCGTCCGGGGCCACGATAATCTCTGCTTTACCATTAGCAGCCCCCAGCGCCTGGCGCATGGTTTGaaactcctcctacgg                               >  2:490818/1‑215 (MQ=255)
                                                                                                                                                                                                        cccGTGTGGTAATTCCCCCCCGTCTTTTCTCATTACAGCGTCTTCTTCCCACAATACTGCTGAAACCATTCCAG‑CATCCGCTGCCAGCCATTTTCTGCAGTGGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGaa                             <  2:695782/228‑1 (MQ=255)
                                                                                                                                                                                                         ccGGGTGTTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCGTCCCCCCATACTGCTTAAACCATTCCAG‑CAGACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGaa                             <  2:273322/228‑1 (MQ=255)
                                                                                                                                                                                                         ccGGGTGGTAATTCCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCCCCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGaa                             <  1:175300/228‑1 (MQ=255)
                                                                                                                                                                                                             gtggtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGACTGGCGCATGGTTTCAACGCCATCCTGCGGAATggt                         >  1:312028/1‑226 (MQ=255)
                                                                                                                                                                                                               ggtAATTCCCCCCGGTCTTTTCCTATTACCGGGCCTTCTTCCCACCAGACTGGTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGtt                      <  2:85006/229‑1 (MQ=255)
                                                                                                                                                                                                               ggtAATACCCACCGGTCTTTTCCCAATCCAGCGACTTCGTCCCACCATTCTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTg                        <  1:243145/227‑1 (MQ=255)
                                                                                                                                                                                                                 tAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGtt                      >  1:85006/1‑227 (MQ=255)
                                                                                                                                                                                                                         cccgtgcTTTTCTCATTCCAGGGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATGCGCTGCCAGCCATCTTCTGCAGATGGGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGacc             <  2:463382/222‑1 (MQ=255)
                                                                                                                                                                                                                         cccGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCCGATGCGGCATGATAGCTCGGGCGCTAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCCCTGCTTTCGCATTAGCACCCCGCAGCCCCTGGCCCCTGGTTTCAACCCTCCCCTTCGGACTGCACTCATCCTGacc             >  2:73784/1‑228 (MQ=255)
                                                                                                                                                                                                                         cccGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGACTGGTTTCAACGCTATCCTGCGGAATGCTGTTATCCTGacc             >  1:237025/1‑228 (MQ=255)
                                                                                                                                                                                                                         cccGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCACCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGacc             >  1:297827/1‑228 (MQ=255)
                                                                                                                                                                                                                         cccGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTGAAACCATTCCAG‑GATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGacc             <  1:623903/228‑1 (MQ=255)
                                                                                                                                                                                                                         cccGGGCTTTTCCCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGacc             <  1:482112/228‑1 (MQ=255)
                                                                                                                                                                                                                              tCTTTTCTCATTACAGCGACTTCTCCCCAACAGACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCAGCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCata       <  2:647516/229‑1 (MQ=255)
                                                                                                                                                                                                                               tttttCTCATTCCCGCGCCGTCTTCCCCCCAGACTGCTTAAACCCATCCAG‑CATACGCGGCCAGCCGTTTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCatat      <  2:13092/228‑1 (MQ=255)
                                                                                                                                                                                                                                   tctcATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGGGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGc  >  1:722936/1‑229 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   
TATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAA‑ACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG‑CATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGC  >  NC_000913/4015122‑4015576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A