Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F169 I0 R1	132	34.5	794130	89.8%	713128	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,499,376	C→A	100%	G883V (GGT→GTT)	ebh ←	extracellular matrix binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,499,376	0	C	A	100.0%	108.7 / NA	37	G883V (GGT→GTT)	ebh	extracellular matrix binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (15/22); total (15/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGT  >  CP000730/1499240‑1499509

gCCTCGATAGTATTTGGCATTTATTCCAACAGGAATATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCGTTACTCATATTAGATGCAACTACTTTATTATAATAAATTACTGCTCTAATTTGTTTAACTAc                                                                                                                                   >  1:161621/1‑141 (MQ=255)

 catCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACa                                                                                                                                  <  2:245545/139‑1 (MQ=255)

   tCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAgg                                                                                                                                <  1:245648/141‑1 (MQ=255)

     gATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGATAGGATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtg                                                                                                                              <  1:91216/141‑1 (MQ=255)

       tAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtggt                                                                                                                            >  1:246659/1‑141 (MQ=255)

        attATTTGGCATTTATCCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTa                                                                                                                           <  1:195505/139‑1 (MQ=255)

                   tttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATacaaca                                                                                                                <  1:57229/141‑1 (MQ=255)

                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACAt                                                                                                               <  2:165130/141‑1 (MQ=255)

                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACAt                                                                                                               <  1:63933/141‑1 (MQ=255)

                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACAt                                                                                                               <  1:103728/141‑1 (MQ=255)

                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCAACGCTTCTTAACTACCGGTGGTATGAATACAACAt                                                                                                               <  2:363237/141‑1 (MQ=255)

                        tCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATc                                                                                                           <  1:35373/141‑1 (MQ=255)

                         ccAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCt                                                                                                          <  1:365476/141‑1 (MQ=255)

                            aCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACGACATAATCTTGc                                                                                                       >  2:222262/1‑141 (MQ=255)

                            aCAGGAGTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCCCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTgg                                                                                                       >  2:16126/1‑140 (MQ=255)

                              cggATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTAGAGGTGGTATGAATACAACATAATCTCGCgg                                                                                                     <  2:241500/140‑1 (MQ=255)

                                  ttattGCTTGTTGTTTCAATATTATCTGCCAAAATAGTAACAGCATTACTCCTACTACAGCCAACTACTTTATTATAATAAACTACTGCTCTAATTTTTTTAACTACAGGTGGTATGAATACAACAGAATCTTGCGGTCGt                                                                                                 <  1:90854/141‑1 (MQ=255)

                                  ttattGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGt                                                                                                 <  1:107909/141‑1 (MQ=255)

                                    attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACCGGTGGTATGAATACAACATAATCTTGCGGTCGtat                                                                                               >  2:7279/1‑141 (MQ=255)

                                    attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGtat                                                                                               >  2:135707/1‑141 (MQ=255)

                                        attgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTa                                                                                           <  2:192231/141‑1 (MQ=255)

                                              ggtggAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTACTATAATAAACTACTGCCCTAATTGGTTTAACTACAGGTGGTATGAATACAACATAATCTTCCGGTCGTATAGTAACTGta                                                                                     >  2:250560/1‑141 (MQ=255)

                                                tggAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGtatt                                                                                   >  2:172582/1‑141 (MQ=255)

                                                 ggAATGTCATCTGGCAAAATCGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGtatta                                                                                  >  1:94887/1‑141 (MQ=255)

                                                  gAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGtattat                                                                                 <  1:344128/141‑1 (MQ=255)

                                                             ggCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCa                                                                      <  1:42131/141‑1 (MQ=255)

                                                                          cAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCg                                                         <  1:214785/141‑1 (MQ=255)

                                                                          cAGCATTACTCATATTAGATGCAACCACTTTATCATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATACACCCCATCAGGTGTACCAGTCg                                                         <  1:304991/141‑1 (MQ=255)

                                                                           aggaTTACTGAAGTTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTAGGCACCCCATCAGGTGTACCAGTCGc                                                        <  2:3805/138‑1 (MQ=255)

                                                                                            aTGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTTCCAGTCGCAAGTATCGTATAACCAg                                       >  1:218676/1‑141 (MQ=255)

                                                                                              gCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGAt                                     >  1:155852/1‑141 (MQ=255)

                                                                                                 actactTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGt                                  >  1:12224/1‑141 (MQ=255)

                                                                                                                  aaCTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATCGTATTTGATCTGCCTCACt                 >  1:325568/1‑141 (MQ=255)

                                                                                                                    ctactGCTCTATTTTGTTTAACTACAGGTTGTTTGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCCGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTac               <  2:188133/141‑1 (MQ=255)

                                                                                                                        tGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTaccacc           >  2:66121/1‑141 (MQ=255)

                                                                                                                              aaTTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTACCGTATAACCAGATGGCATTTGATCTGCCCCACTCCCACCATCCGt     >  2:196978/1‑141 (MQ=255)

                                                                                                                                 ttGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGt  <  2:94887/141‑1 (MQ=255)

GCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGT  >  CP000730/1499240‑1499509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A