Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F177 I0 R1
|
51 |
26.9 |
575130 |
96.6% |
555575 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
2,274,965 |
G→C |
100% |
E106D (GAG→GAC) |
bglG → |
transcriptional antiterminator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,274,965 | 0 | G | C | 100.0%
| 80.9
/ NA
| 24 | E106D (GAG→GAC) | bglG | transcriptional antiterminator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (10/14); total (10/14) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CAAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTACT > CP000730/2274827‑2275101
|
caaaAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAccc > 1:254535/1‑141 (MQ=255)
aGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTaa > 1:163824/1‑141 (MQ=255)
gATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGaa > 1:169074/1‑141 (MQ=255)
aCGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTACAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTg < 1:222350/141‑1 (MQ=255)
cGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGt < 2:112249/141‑1 (MQ=255)
tttGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCa < 2:193216/141‑1 (MQ=255)
ttGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGCCGTTTCTGTCCAg > 2:53213/1‑141 (MQ=255)
aGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACtt < 2:22940/141‑1 (MQ=255)
aGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACtt < 1:229425/141‑1 (MQ=255)
aaTCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTCCTGTCCAGACTTTAg < 1:212985/141‑1 (MQ=255)
tCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCAGTCCAGACTTTAGCa < 1:213282/141‑1 (MQ=255)
caTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgat > 2:68400/1‑141 (MQ=255)
aCCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTa < 2:220158/141‑1 (MQ=255)
ttGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGc < 2:167428/141‑1 (MQ=255)
ttGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATGGGCGTTTCTGTCCGGACTTTAGCAAAGATGTTAGATGATTTAGGGc > 1:44222/1‑141 (MQ=255)
tGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTaaa > 2:42752/1‑141 (MQ=255)
agCAGAAAGCAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTAcc < 1:25541/141‑1 (MQ=255)
gCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCa > 1:109182/1‑141 (MQ=255)
tataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTAATCTCGaa > 1:146958/1‑141 (MQ=255)
tataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTAGCGTAAGCTCGaa > 2:58439/1‑141 (MQ=255)
cGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCg < 1:48928/141‑1 (MQ=255)
cTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTg > 2:66073/1‑141 (MQ=255)
aTACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGaa < 1:100856/141‑1 (MQ=255)
aGGACCCCGTTAAACAATGTAGTTTAGCGCAAGAAATTGTGCTTTCTGTCCACACTTTAGTAAACAGGTTAGATGAATTATGGCTTGATTTAAATAATTACCTTCTATTTTGATCTCGTAAGCTTGTCTAAGGCATTTACt < 2:10673/141‑1 (MQ=255)
aGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCGCGAAAGCGTAGCGAAGGCATTTACt < 1:143909/141‑1 (MQ=255)
|
CAAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTACT > CP000730/2274827‑2275101
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
