Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F106 I0 R1	421	125.2	4599244	98.2%	4516457	137.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,074,894	G→T	6.0%	intergenic (+14/+26)	rutR → / ← putA	rut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,074,894	0	G	T	6.0%	235.8 / 5.1	67	intergenic (+14/+26)	rutR/putA	rut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (28/35); new base T (2/2); total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAC  >  NC_000913/1074746‑1075039

tCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGgaggag                                                                                                                                                   <  4:726131/149‑1 (MQ=255)

   aCTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGNGGtt                                                                                                                                                <  3:1079659/149‑1 (MQ=255)

    cTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGtta                                                                                                                                               >  2:663620/1‑148 (MQ=255)

     tCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGt                                                                                                                                              <  2:898010/149‑1 (MQ=255)

     tCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGt                                                                                                                                              <  3:332988/149‑1 (MQ=255)

       aaCATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTaa                                                                                                                                            >  1:17862/1‑149 (MQ=255)

       aaCATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTaa                                                                                                                                            <  3:818909/149‑1 (MQ=255)

         cATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGttacaacc                                                                                                                                          <  1:663620/149‑7 (MQ=255)

          aTTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAt                                                                                                                                         >  1:1086183/1‑149 (MQ=255)

           ttACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtc                                                                                                                                        <  1:47239/149‑1 (MQ=255)

              cGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctc                                                                                                                                     <  2:1086183/149‑1 (MQ=255)

               gCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctcc                                                                                                                                    <  1:431907/149‑1 (MQ=255)

                        gCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTg                                                                                                                           >  3:168953/1‑149 (MQ=255)

                         ccccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGt                                                                                                                          >  2:231088/1‑149 (MQ=255)

                          cccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGt                                                                                                                                              >  4:974220/1‑128 (MQ=255)

                          cccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGt                                                                                                                                              <  3:974220/128‑1 (MQ=255)

                          cccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGtt                                                                                                                         >  2:558150/1‑149 (MQ=255)

                            cTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTa                                                                                                                       >  1:379621/1‑149 (MQ=255)

                             tCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTg                                                                                                                                               <  3:326793/124‑1 (MQ=255)

                             tCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTg                                                                                                                                               >  4:326793/1‑124 (MQ=255)

                             tCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTaa                                                                                                                      >  1:202421/1‑149 (MQ=255)

                              cAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAAc                                                                                                                     <  1:309806/149‑1 (MQ=255)

                              cAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAAc                                                                                                                     <  2:202421/149‑1 (MQ=255)

                                ggTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCt                                                                                                                   <  2:28073/149‑1 (MQ=255)

                                  tGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCtat                                                                                                                 <  4:159998/149‑1 (MQ=255)

                                   ggAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCtata                                                                                                                <  4:467120/149‑1 (MQ=255)

                                       gCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCa                                                                                                            >  3:1015197/1‑149 (MQ=255)

                                        cGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAt                                                                                                           <  1:350700/149‑1 (MQ=255)

                                         ggTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAtt                                                                                                          >  1:145041/1‑149 (MQ=255)

                                           tGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTaa                                                                                                        <  1:583340/149‑1 (MQ=255)

                                           tGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTaa                                                                                                        <  3:321743/149‑1 (MQ=255)

                                               aGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGtta                                                                                                                                               >  2:482054/1‑105 (MQ=255)

                                               aGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGtta                                                                                                                                               <  1:482054/106‑2 (MQ=255)

                                                 gcgcGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGc                                                                                                  <  1:1004015/149‑1 (MQ=255)

                                                 gcgcGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGc                                                                                                  <  2:145041/149‑1 (MQ=255)

                                                             gcgATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGGTAACGCcagc                                                                                      <  4:1015197/149‑1 (MQ=255)

                                                                     gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctcc                                                                                                                                    <  4:781178/95‑1 (MQ=255)

                                                                     gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctcc                                                                                                                                    >  3:781178/1‑95 (MQ=255)

                                                                     gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcgg                                                                              >  1:353555/1‑149 (MQ=255)

                                                                     gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcgg                                                                              >  1:715847/1‑149 (MQ=255)

                                                                     gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcgg                                                                              >  1:674447/1‑149 (MQ=255)

                                                                            tcaatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTcac                                                                       <  1:558150/149‑1 (MQ=255)

                                                                                 caaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCa                                                                  <  3:765152/149‑1 (MQ=255)

                                                                                 caaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCa                                                                  <  3:173013/149‑1 (MQ=255)

                                                                                  aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAg                                                                 >  1:1100878/1‑149 (MQ=255)

                                                                                  aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAg                                                                 >  3:437621/1‑149 (MQ=255)

                                                                                       gTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAc                                                            <  2:353555/149‑1 (MQ=255)

                                                                                          gAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCt                                                         >  1:1049566/1‑149 (MQ=255)

                                                                                           aaaaCGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTc                                                        >  4:878661/1‑149 (MQ=255)

                                                                                                 tGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGAtata                                                  <  2:379621/149‑1 (MQ=255)

                                                                                                            attattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTAGCTGTTTAACCTATAGTCATTAAGATGCCGTTACCGCCAGAGGCAGCGTTATTCACACCCAGCCCACGCGCTATATACTTCTGTTCCa                                       <  4:21530/149‑1 (MQ=255)

                                                                                                            attattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAtt                                                                          <  2:1133548/114‑1 (MQ=255)

                                                                                                            attattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAtt                                                                          >  1:1133548/1‑114 (MQ=255)

                                                                                                            attattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCa                                       <  2:17862/149‑1 (MQ=255)

                                                                                                                ttGAGGGGATTCGACCACGTTAAAGATGCCGGGGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAg                                   >  2:248934/1‑149 (MQ=255)

                                                                                                                ttGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGtt                                                                                               >  3:427930/1‑89 (MQ=255)

                                                                                                                ttGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGtt                                                                                               <  4:427930/89‑1 (MQ=255)

                                                                                                                     gggATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGatat                              <  4:437621/149‑1 (MQ=255)

                                                                                                                      ggATTCGACCACGGTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGGATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATAtt                             >  1:182750/1‑149 (MQ=255)

                                                                                                                       gATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTg                            >  3:313232/1‑149 (MQ=255)

                                                                                                                             aCCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCg                      >  3:407316/1‑149 (MQ=255)

                                                                                                                               cACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca                                                                        >  3:755350/1‑97 (MQ=255)

                                                                                                                               cACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca                                                                        <  4:755350/97‑1 (MQ=255)

                                                                                                                                  gTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACg                 >  1:302637/1‑149 (MQ=255)

                                                                                                                                            ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAccc       >  3:879563/1‑149 (MQ=255)

                                                                                                                                             cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt      <  4:168953/149‑1 (MQ=255)

                                                                                                                                             cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt      <  2:715847/149‑1 (MQ=255)

                                                                                                                                             cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt      <  2:1100878/149‑1 (MQ=255)

                                                                                                                                                 ggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAtt                                                                          <  2:522724/77‑1 (MQ=255)

                                                                                                                                                 ggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAtt                                                                          >  1:522724/1‑77 (MQ=255)

                                                                                                                                                 ggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAc  <  1:231088/149‑1 (MQ=255)

TCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAC  >  NC_000913/1074746‑1075039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A