Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F34 I0 R2 578 165.1 5883352 98.2% 5777451 141.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,888,034 A→T 6.2% intergenic (‑43/+27) rnd ← / ← fadD ribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,888,0340AT 6.2% 452.2 / 16.1 128intergenic (‑43/+27)rnd/fadDribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base A (54/66);  new base T (3/5);  total (57/71)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 128 total aligned reads displayed.

GCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCC  >  NC_000913/1887887‑1888180
                                                                                                                                                      |                                                                                                                                                   
gcgcGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑c                                                                                                                                                   <  2:546281/149‑1 (MQ=255)
gcgcGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑c                                                                                                                                                   <  1:205035/149‑1 (MQ=255)
      aCGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGTTAAT‑CCGGCGt                                                                                                                                             <  2:564433/149‑1 (MQ=255)
      aCGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGt                                                                                                                                             >  2:711237/1‑149 (MQ=255)
        gAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACTGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCt                                                                                                                                           >  4:857719/1‑149 (MQ=255)
        gAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGTTAAT‑CCGGCGttt                                                                                                                                           <  3:95375/149‑3 (MQ=255)
         aaCAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGTTAAT‑CCGGCGttt                                                                                                                                           <  4:1402324/148‑3 (MQ=255)
         aaCAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTg                                                                                                                                          >  3:398395/1‑149 (MQ=255)
         aaCAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGG‑TTAATCCGGCGttt                                                                                                                                           >  3:1402324/1‑146 (MQ=255)
          aCAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTga                                                                                                                                         >  2:759437/1‑149 (MQ=255)
            aaaTTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTATTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTgacg                                                                                                                                       >  1:1033294/1‑149 (MQ=255)
            aaaTTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAAAGCCGGGTTTA‑ACGGCGGCTtacg                                                                                                                                       >  1:287612/1‑149 (MQ=255)
             aaTTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGTTAAT‑CCGGCGtttttttt                                                                                                                                      <  1:127852/149‑8 (MQ=255)
              aTTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTgacgac                                                                                                                                     >  3:368192/1‑149 (MQ=255)
               ttCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTg                                                                                                                                          <  1:579509/143‑1 (MQ=255)
               ttCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACgact                                                                                                                                    >  3:1036202/1‑149 (MQ=255)
                 cAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACgactga                                                                                                                                  <  4:329054/149‑1 (MQ=255)
                 cAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACgactga                                                                                                                                  <  3:1440246/149‑1 (MQ=255)
                      tCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTaa                                                                                                                             >  3:1415562/1‑149 (MQ=255)
                      tCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTaa                                                                                                                             >  4:1210383/1‑149 (MQ=255)
                      tCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTaa                                                                                                                             <  1:1193348/149‑1 (MQ=255)
                       ccAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAAc                                                                                                                            <  3:136134/149‑1 (MQ=255)
                       ccAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACCACTGACTTAAc                                                                                                                            <  4:134849/149‑1 (MQ=255)
                        cAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACg                                                                                                                           >  3:354940/1‑149 (MQ=255)
                         aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTCAAAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACg                                                                                                                           >  2:950455/1‑149 (MQ=255)
                         aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGTTAAT‑CCGGCGtt                                                                                                                                            <  4:519810/131‑2 (MQ=255)
                         aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGTTAAT‑CCGGCGtt                                                                                                                                            >  3:519810/1‑130 (MQ=255)
                         aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑cc                                                                                                                                                  <  1:1030519/125‑1 (MQ=255)
                         aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGc                                                                                                                          >  4:1189855/1‑149 (MQ=255)
                         aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGc                                                                                                                          >  2:1410321/1‑149 (MQ=255)
                            gCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCa                                                                                                                       <  1:806711/149‑1 (MQ=255)
                            gCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCa                                                                                                                       <  4:664885/149‑1 (MQ=255)
                               aTCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGc                                                                                                                    <  1:711237/149‑1 (MQ=255)
                                tCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCt                                                                                                                   >  4:522195/1‑149 (MQ=255)
                                tCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCt                                                                                                                   >  3:292561/1‑149 (MQ=255)
                                   ccGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTa                                                                                                                <  4:211643/149‑1 (MQ=255)
                                     ggAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTAtt                                                                                                              <  4:798150/149‑1 (MQ=255)
                                     ggAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTAtt                                                                                                              <  3:1189855/149‑1 (MQ=255)
                                        aaagCACGGACCGGTTTACAAAAAGGAGCCAAGGCATCCGTCGGTGGTAAT‑ATTTGGTTATTCAAATTTTTTTCCTTTTTGGGGGGCTCA‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGt                                                                                                            <  2:287612/146‑1 (MQ=255)
                                         aGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTcc                                                                                                          <  3:1366205/149‑1 (MQ=255)
                                            cACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACt                                                                                                       <  3:610035/149‑1 (MQ=255)
                                                gacggCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCACGCTTTATTGTCCACTTTGc                                                                                                   <  3:857719/149‑1 (MQ=255)
                                                    gCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcg                                                                                               >  4:771640/1‑149 (MQ=255)
                                                     cTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcgc                                                                                              >  1:810444/1‑149 (MQ=255)
                                                          cacaAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                         <  4:1368415/149‑1 (MQ=255)
                                                          cacaAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                         <  4:379531/149‑1 (MQ=255)
                                                          cacaAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                         <  2:397292/149‑1 (MQ=255)
                                                           acaAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTga                                                                                                                                         >  3:1158006/1‑100 (MQ=255)
                                                           acaAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTAt                                                                                                               >  4:1278528/1‑126 (MQ=255)
                                                           acaAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTAt                                                                                                               <  3:1278528/126‑1 (MQ=255)
                                                           acaAAGAAGCCAGCGCAT‑CCTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTga                                                                                                                                         <  4:1158006/100‑1 (MQ=255)
                                                            caAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGt                                                                                       >  3:47032/1‑149 (MQ=255)
                                                             aaaGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGTCTTTACTGTCCACTTTGCCGCGCGCTTCGTc                                                                                      >  1:297527/1‑149 (MQ=255)
                                                             aaaGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTc                                                                                      <  3:247228/149‑1 (MQ=255)
                                                                   gCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTaa                                                                                <  4:511442/149‑1 (MQ=255)
                                                                    ccAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAAt                                                                               >  3:1432586/1‑149 (MQ=255)
                                                                           aT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtc                                                                        <  1:872478/149‑1 (MQ=255)
                                                                           aT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtc                                                                        <  2:810444/149‑1 (MQ=255)
                                                                            t‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTCAAAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtc                                                                        >  3:646947/1‑149 (MQ=255)
                                                                               gTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCa                                                                     >  4:429798/1‑149 (MQ=255)
                                                                                tCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCaa                                                                    >  1:874061/1‑149 (MQ=255)
                                                                                       tAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                             <  3:309640/149‑1 (MQ=255)
                                                                                       tAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTGATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                             <  3:771640/149‑1 (MQ=255)
                                                                                       tAAGCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                             >  1:1275043/1‑149 (MQ=255)
                                                                                        aaTCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                                            <  3:43792/149‑1 (MQ=255)
                                                                                        aaTCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                                            >  3:1022650/1‑149 (MQ=255)
                                                                                         aTCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTcc                                                           <  3:314357/149‑1 (MQ=255)
                                                                                            aTTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                        >  4:400298/1‑149 (MQ=255)
                                                                                            aTTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                        >  4:467028/1‑149 (MQ=255)
                                                                                             tttGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACg                                                       >  4:1100213/1‑149 (MQ=255)
                                                                                             tttGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑‑AAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGt                                                      >  3:746997/1‑149 (MQ=255)
                                                                                              ttGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGt                                                      <  2:1065847/149‑1 (MQ=255)
                                                                                              ttGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGt                                                      <  2:1472823/149‑1 (MQ=255)
                                                                                               tGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGtt                                                     <  4:109730/149‑1 (MQ=255)
                                                                                                ggTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTa                                                    >  2:548673/1‑149 (MQ=255)
                                                                                                      tCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTc                                              <  2:1450600/149‑1 (MQ=255)
                                                                                                       cAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCg                                             <  1:1305428/149‑1 (MQ=255)
                                                                                                        aaaTTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgt                                                                         <  4:332557/120‑1 (MQ=255)
                                                                                                          aTTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                            >  4:359809/1‑147 (MQ=255)
                                                                                                          aTTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTa                                          <  3:650234/149‑1 (MQ=255)
                                                                                                           ttGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTaa                                         <  4:1130143/149‑1 (MQ=255)
                                                                                                              tttt‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCg                                             >  2:28454/1‑142 (MQ=255)
                                                                                                              tttt‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCg                                             <  1:28454/142‑1 (MQ=255)
                                                                                                                 t‑ctcTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatc                                   <  1:1223078/149‑1 (MQ=255)
                                                                                                                   ctctTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca                                  <  1:1051718/149‑1 (MQ=255)
                                                                                                                    tctTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc                                 <  3:1210383/149‑1 (MQ=255)
                                                                                                                        tAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGaaa                             <  1:1253086/149‑1 (MQ=255)
                                                                                                                        tAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGaaa                             <  2:1417940/149‑1 (MQ=255)
                                                                                                                             ggCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTcca                        >  1:632518/1‑149 (MQ=255)
                                                                                                                                     aaaaaaaCGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCtt                 >  1:1309730/1‑149 (MQ=255)
                                                                                                                                       tcagaCGCCGGTTAAT‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCg               >  3:343193/5‑149 (MQ=255)
                                                                                                                                        aaaaCGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCgg              >  3:337319/1‑149 (MQ=255)
                                                                                                                                           aCGCCGGTTAAT‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGTCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTAc           >  3:1432330/1‑149 (MQ=255)
                                                                                                                                             gCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACtt         <  1:1040465/149‑1 (MQ=255)
                                                                                                                                              ccGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACttt        <  3:429798/149‑1 (MQ=255)
                                                                                                                                              ccGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACttt        <  3:467028/149‑1 (MQ=255)
                                                                                                                                                ggATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGt      >  3:1010610/1‑149 (MQ=255)
                                                                                                                                                 gATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTa     >  2:1471429/1‑149 (MQ=255)
                                                                                                                                                 gATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTa     >  2:1467188/1‑149 (MQ=255)
                                                                                                                                                  aTTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGGAt    <  2:458507/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCAT‑CGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTT‑CTCTTTAGTGGGCGTC‑AAAAAAAACGCCGGATTAA‑CCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCC  >  NC_000913/1887887‑1888180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A