Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A23 F41 I0 R1 255 66.8 2308880 98.8% 2281173 145.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,936,823 A→G 5.1% M58V (ATG→GTG)  yebK → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,936,8230AG 5.1% 261.0 / 3.4 79M58V (ATG→GTG) yebKputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (34/41);  new base G (2/2);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

TGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATGGCAACGCTT  >  NC_000913/1936683‑1936969
                                                                                                                                            |                                                                                                                                                  
tGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACAcg                                                                                                                                            >  2:91018/1‑149 (MQ=255)
      aTTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGt                                                                                                                                      >  2:582184/1‑149 (MQ=255)
          gAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTc                                                                                                                                  <  4:279283/149‑1 (MQ=255)
          gAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTc                                                                                                                                  >  1:57398/1‑149 (MQ=255)
           aGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTcc                                                                                                                                 <  2:180207/149‑1 (MQ=255)
           aGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTcc                                                                                                                                 <  3:416381/149‑1 (MQ=255)
            gCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCt                                                                                                                                >  1:533513/1‑149 (MQ=255)
             cAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTg                                                                                                                               >  4:155271/1‑149 (MQ=255)
             cAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTg                                                                                                                               >  3:424503/1‑149 (MQ=255)
              aaaTCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGa                                                                                                                              >  2:291746/1‑149 (MQ=255)
              aaaTCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGa                                                                                                                              <  1:354948/149‑1 (MQ=255)
              aaaTCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGa                                                                                                                              <  2:228581/149‑1 (MQ=255)
               aagcatagCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATGCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGAt                                                                                                                             >  4:351125/7‑149 (MQ=255)
                  cAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGAtttt                                                                                                                          >  1:481853/1‑149 (MQ=255)
                     agCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTaaa                                                                                                                       <  3:255169/149‑1 (MQ=255)
                     agCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTaaa                                                                                                                       <  2:403310/149‑1 (MQ=255)
                     agCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTaaa                                                                                                                       <  2:121019/149‑1 (MQ=255)
                     agCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTaaa                                                                                                                       <  3:300773/149‑1 (MQ=255)
                     agCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTaaa                                                                                                                       <  1:17775/149‑1 (MQ=255)
                               tGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTgg                                                                                                             <  1:321740/149‑1 (MQ=255)
                               tGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTgg                                                                                                             <  1:291746/149‑1 (MQ=255)
                                  cGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGcac                                                                                                          <  4:65907/149‑1 (MQ=255)
                                         aTTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACaga                                                                                                       >  1:121486/1‑145 (MQ=255)
                                         aTTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACaga                                                                                                       <  2:121486/145‑1 (MQ=255)
                                          ttCTGGCTTCGCCCGTTAACGCGAGCCATTTGAGTCTTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGGCGGTGAATCGCTTCTGTCGCAGCATGGACACGCGCGGCTTTCCTAATAGTAAACTTCATCTGGCACAGAGTCTg                                                                                                  >  4:351117/1‑149 (MQ=255)
                                          ttCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTg                                                                                                  >  2:585884/1‑149 (MQ=255)
                                                cTTCGCCCGATCACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAAt                                                                                            <  3:367923/149‑1 (MQ=255)
                                                 ttCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATg                                                                                           <  1:214019/149‑1 (MQ=255)
                                                    gCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGACa                                                                                        >  2:262621/1‑149 (MQ=255)
                                                      ccGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCGTGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACt                                                                                      >  3:296425/1‑149 (MQ=255)
                                                      ccGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCGTGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACt                                                                                      >  3:56986/1‑149 (MQ=255)
                                                      ccGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACt                                                                                      <  1:438750/149‑1 (MQ=255)
                                                      ccGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACt                                                                                      >  1:19714/1‑149 (MQ=255)
                                                        gATAACGCTATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTATGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTcc                                                                                    <  3:351117/149‑1 (MQ=255)
                                                        gATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTcc                                                                                    >  1:241729/1‑149 (MQ=255)
                                                        gATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTcc                                                                                    >  4:564274/1‑149 (MQ=255)
                                                        gATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTcc                                                                                    >  1:497401/1‑149 (MQ=255)
                                                         aTAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTccc                                                                                   >  4:286529/1‑149 (MQ=255)
                                                         aTAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTccc                                                                                   >  4:232806/1‑149 (MQ=255)
                                                           aaCGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTa                                                                                 <  3:426812/149‑1 (MQ=255)
                                                              gcgATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGt                                                                              <  2:481853/149‑1 (MQ=255)
                                                                           gTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTc                                                                 >  4:272298/1‑149 (MQ=255)
                                                                           gTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTc                                                                 >  3:321467/1‑149 (MQ=255)
                                                                           gTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTc                                                                 >  3:500287/1‑149 (MQ=255)
                                                                           gTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTc                                                                 <  2:10321/149‑1 (MQ=255)
                                                                              ttGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAAt                                                              <  2:237098/149‑1 (MQ=255)
                                                                              ttGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAAt                                                              <  4:282090/149‑1 (MQ=255)
                                                                                   gctATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGa                                                         <  4:451713/149‑1 (MQ=255)
                                                                                     tATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCaa                                                                     >  2:100139/1‑135 (MQ=255)
                                                                                     tATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCaa                                                                     <  1:100139/135‑1 (MQ=255)
                                                                                        ggCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACa                                                    >  3:284813/1‑149 (MQ=255)
                                                                                           aCTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCg                                                 >  3:163185/1‑149 (MQ=255)
                                                                                            cTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGt                                                >  2:100024/1‑149 (MQ=255)
                                                                                             tGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGtt                                               <  4:284813/149‑1 (MQ=255)
                                                                                             tGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGtt                                               <  2:433909/149‑1 (MQ=255)
                                                                                                aaGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGaa                                            <  1:100024/149‑1 (MQ=255)
                                                                                                  gCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATc                                          >  4:541963/1‑149 (MQ=255)
                                                                                                  gCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATc                                          <  2:370786/149‑1 (MQ=255)
                                                                                                       tGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATaca                                     >  2:238984/1‑149 (MQ=255)
                                                                                                          tAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAg                                  <  4:335496/149‑1 (MQ=255)
                                                                                                          tAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAg                                  <  2:258222/149‑1 (MQ=255)
                                                                                                           aGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTc                                                                 >  3:358919/1‑117 (MQ=255)
                                                                                                           aGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTc                                                                 <  4:358919/117‑1 (MQ=255)
                                                                                                            gCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAggg                                <  4:366985/149‑1 (MQ=255)
                                                                                                            gCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAggg                                >  2:480521/1‑149 (MQ=255)
                                                                                                            gCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAggg                                >  1:251543/1‑149 (MQ=255)
                                                                                                                    aCGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATAtt                        <  3:155271/149‑1 (MQ=255)
                                                                                                                     cGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATAttt                       <  3:212570/149‑1 (MQ=255)
                                                                                                                       gTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGa                     <  4:163185/149‑1 (MQ=255)
                                                                                                                       gTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGa                     <  3:286529/149‑1 (MQ=255)
                                                                                                                       gTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGa                     <  3:232806/149‑1 (MQ=255)
                                                                                                                        tGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATa                                       <  3:551956/130‑1 (MQ=255)
                                                                                                                        tGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATa                                       >  4:551956/1‑130 (MQ=255)
                                                                                                                            tCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCg                >  3:47173/1‑149 (MQ=255)
                                                                                                                            tCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCg                >  1:471998/1‑149 (MQ=255)
                                                                                                                                 tCTGTCGCAGCGTGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATg           <  4:296425/149‑1 (MQ=255)
                                                                                                                                 tCTGTCGCAGCGTGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATg           <  4:56986/149‑1 (MQ=255)
                                                                                                                                      cGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATGGCAAc      <  2:533513/149‑1 (MQ=255)
                                                                                                                                      cGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATGGCAAc      <  1:480521/149‑1 (MQ=255)
                                                                                                                                        cagcaTGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATGGCAACGc    <  4:75368/149‑1 (MQ=255)
                                                                                                                                          gcaTGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATGGCAACGCtt  >  2:371404/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
TGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATGGCAACGCTT  >  NC_000913/1936683‑1936969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A