Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A23 F41 I0 R1 255 66.8 2308880 98.8% 2281173 145.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,259,700 C→T 9.2% intergenic (‑404/+19) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,259,7000CT 9.2% 197.1 / 8.3 65intergenic (‑404/+19)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base T (3/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAG  >  NC_000913/2259552‑2259847
                                                                                                                                                     |                                                                                                                                                   
taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGc                                                                                                                                                     >  4:381388/1‑149 (MQ=255)
   tCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCt                                                                                                                                                  <  1:206488/149‑1 (MQ=255)
     tGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGt                                                                                                                                                >  1:86167/1‑149 (MQ=255)
            gAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAacacac                                                                                                                                         >  4:79611/1‑149 (MQ=255)
                   tAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTttat                                                                                                                                  <  2:446906/149‑1 (MQ=255)
                       cTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACg                                                                                                                              >  3:64158/1‑149 (MQ=255)
                                  atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTC‑CTGTCCTGTAac                                                                                                                                             >  2:15079/1‑123 (MQ=38)
                                  atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCA‑GAAATTTCCTGTCCTGTAac                                                                                                                                             <  1:15079/123‑1 (MQ=38)
                                     aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGTCAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACt                                                                                                                <  4:197969/149‑1 (MQ=255)
                                     aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACt                                                                                                                >  1:428226/1‑149 (MQ=255)
                                     aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACt                                                                                                                <  1:494811/149‑1 (MQ=255)
                                      aCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTg                                                                                                               <  2:329117/149‑1 (MQ=255)
                                       cGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGc                                                                                                              <  2:431787/149‑1 (MQ=255)
                                        gTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCg                                                                                                             >  1:315405/1‑149 (MQ=255)
                                         tttGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGt                                                                                                            <  2:24854/149‑1 (MQ=255)
                                             aaGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCAc                                                                                                        <  4:47915/149‑1 (MQ=255)
                                               gTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTttatt                                                                                                                                 >  3:204524/1‑122 (MQ=255)
                                               gTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTttatt                                                                                                                                 <  4:204524/122‑1 (MQ=255)
                                               ctttCTACTGAAAGATAAACCCATTTAAATTCATAAAATTACAATCACAAACCGGTCATTCTCTTTGCGCATCTTTAATATTACATTACCGGAAATTT‑CTGCCCTGTCACACATATTTTATTACGCTCCTTCCGCTACTGTGTCCACtt                                                                                                      >  4:513571/2‑149 (MQ=255)
                                                       tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGt                                                                                              <  4:73035/149‑1 (MQ=255)
                                                       tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGt                                                                                              >  4:55926/1‑149 (MQ=255)
                                                       tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGt                                                                                              >  4:109353/1‑149 (MQ=255)
                                                        gAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGtt                                                                                             <  3:505925/149‑1 (MQ=255)
                                                           aGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCa                                                                                          <  4:338001/149‑1 (MQ=255)
                                                                aaaCCATTTAAATTCATAAAATTACATTTACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg                                                                                     >  4:203656/1‑149 (MQ=255)
                                                                 aaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAgg                                                                                    >  3:260109/1‑149 (MQ=255)
                                                                  aCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACATGACAGGAAATTT‑CTTCCCTGTAACACACATTTTATTATGCTGCATTCGCTACTGCGTCCCCTTCCGGACGTTTCAGGAAGGc                                                                                   >  4:45231/1‑149 (MQ=255)
                                                                  aCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGc                                                                                   <  1:546082/149‑1 (MQ=255)
                                                                     aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTcc                                                                                                          <  4:248711/123‑1 (MQ=255)
                                                                     aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTcc                                                                                                          >  3:248711/1‑123 (MQ=255)
                                                                     aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATa                                                                                >  2:430444/1‑149 (MQ=255)
                                                                      tttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAg                                                                               <  4:6466/149‑1 (MQ=255)
                                                                       ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAgg                                                                              <  3:357603/149‑1 (MQ=255)
                                                                           aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa                                                                          >  3:148388/1‑149 (MQ=255)
                                                                            ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaaa                                                                         <  1:219262/149‑1 (MQ=255)
                                                                              cATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAcc                                                                       >  3:62119/1‑149 (MQ=255)
                                                                               aTAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAccc                                                                      >  2:438811/1‑149 (MQ=255)
                                                                                    aTTACATTCACAAACCGGTCTTTCTCTTTGCGCTCATCAAACGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccac                                                                 >  3:370236/1‑149 (MQ=255)
                                                                                         aTTCACAACCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCg                                                            <  2:280244/149‑1 (MQ=255)
                                                                                         aTTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCg                                                            >  1:12859/1‑149 (MQ=255)
                                                                                             acaAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg                                                                                     >  1:571690/1‑120 (MQ=255)
                                                                                             acaAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg                                                                                     <  2:571690/120‑1 (MQ=255)
                                                                                              caAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCg                                                       >  1:81967/1‑149 (MQ=255)
                                                                                                aaCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGc                                                     >  2:556891/1‑149 (MQ=255)
                                                                                                     gTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataa                                                >  1:239597/1‑149 (MQ=255)
                                                                                                       cATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAAtt                                              <  3:63396/149‑1 (MQ=255)
                                                                                                        aTTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTg                                             >  3:186960/1‑149 (MQ=255)
                                                                                                              tttGCGCTCATCAAATGTTACAGGGCAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa                                       <  2:153387/149‑1 (MQ=255)
                                                                                                              tttGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa                                       <  2:275898/149‑1 (MQ=255)
                                                                                                              tttGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa                                       >  1:575239/1‑149 (MQ=255)
                                                                                                                 gcgcTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGt                                    <  4:348523/149‑1 (MQ=255)
                                                                                                                   gcTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTaa                                  >  2:278380/1‑149 (MQ=255)
                                                                                                                      catcaAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAccc                               <  1:4321/149‑1 (MQ=255)
                                                                                                                             tGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCg                        <  2:224020/149‑1 (MQ=255)
                                                                                                                               ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc                      >  2:360185/1‑149 (MQ=255)
                                                                                                                               ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc                      <  3:504131/149‑1 (MQ=255)
                                                                                                                               ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc                      <  1:360185/149‑1 (MQ=255)
                                                                                                                               ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc                      >  4:504131/1‑149 (MQ=255)
                                                                                                                                    ggacaggaAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCGTAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt                 >  4:320742/1‑149 (MQ=255)
                                                                                                                                         ggaAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAgcgc            >  1:359456/1‑149 (MQ=255)
                                                                                                                                          gaAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACTCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGcc           >  2:99683/1‑149 (MQ=255)
                                                                                                                                            aaTTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAg         <  1:556891/149‑1 (MQ=255)
                                                                                                                                                  cTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATc    <  4:381618/149‑1 (MQ=255)
                                                                                                                                                  cTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATACCGCCAGGGATc    <  3:320742/149‑1 (MQ=255)
                                                                                                                                                   tGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATca   <  1:430444/149‑1 (MQ=255)
                                                                                                                                                    gCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATcag  <  1:278380/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAG  >  NC_000913/2259552‑2259847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A