Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F71 I0 R1	810	98.9	3562578	97.8%	3484201	143.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,383,959	A→T	12.5%	intergenic (+35/+36)	elaD → / ← yfbK	protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,383,959	0	A	T	12.5%	215.4 / 21.0	73	intergenic (+35/+36)	elaD/yfbK	protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (32/31); new base T (4/5); total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

AGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAT  >  NC_000913/2383811‑2384102

aGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAata                                                                                                                                                 <  3:627196/149‑1 (MQ=255)

aGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAata                                                                                                                                                 >  2:562585/1‑149 (MQ=255)

  aaaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATg                                                                                                                                               <  3:786459/149‑1 (MQ=255)

   aaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGc                                                                                                                                              <  1:373919/149‑1 (MQ=255)

    aTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCt                                                                                                                                             >  1:604146/1‑149 (MQ=255)

       gTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGAc                                                                                                                                          <  1:552596/149‑1 (MQ=255)

         tATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTg                                                                                                                                        <  2:432638/149‑1 (MQ=255)

         tATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTg                                                                                                                                        <  4:498831/149‑1 (MQ=255)

         tATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCAACCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGCCAGCAAATATGCTGACTg                                                                                                                                        <  3:321328/149‑1 (MQ=255)

          aTTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTgg                                                                                                                                       <  3:870181/149‑1 (MQ=255)

           tttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGt                                                                                                                                      >  2:196192/1‑149 (MQ=255)

                   aaTATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATa                                                                                                                              >  1:300616/1‑149 (MQ=255)

                    atatGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAg                                                                                                                             >  1:707921/1‑149 (MQ=255)

                         gCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGc                                                                                                                        <  1:524687/149‑1 (MQ=255)

                             tGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTaaa                                                                                                                    <  4:472026/149‑1 (MQ=255)

                              gCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTgg                                                                                                                                       >  2:778017/1‑129 (MQ=255)

                              gCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTgg                                                                                                                                       <  1:778017/129‑1 (MQ=255)

                               cAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACa                                                                                                                  <  1:196192/149‑1 (MQ=255)

                                  atatCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtc                                                                                                               <  3:15602/149‑1 (MQ=255)

                                  atatCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtc                                                                                                               <  3:706563/149‑1 (MQ=255)

                                         tcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactg                                                                                                        >  1:764389/1‑149 (MQ=255)

                                          caTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATCTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactga                                                                                                       <  3:519332/149‑1 (MQ=255)

                                           attattATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactgac                                                                                                      <  3:777303/149‑1 (MQ=255)

                                                 ataGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTGTGCTAAACAGTCATCACTGACTGATgt                                                                                                <  2:129640/149‑1 (MQ=255)

                                                  taGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtc                                                                                               >  1:748549/1‑149 (MQ=255)

                                                   aGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                              >  4:683128/1‑149 (MQ=255)

                                                     aaaCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                            <  2:323905/149‑1 (MQ=255)

                                                     aaaCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                            >  3:701132/1‑149 (MQ=255)

                                                         ttAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTcaca                                                                                        >  1:494601/1‑149 (MQ=255)

                                                         ttAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTcaca                                                                                        <  3:845317/149‑1 (MQ=255)

                                                            gAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCa                                                                                     <  1:564168/149‑1 (MQ=255)

                                                              aaTAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATc                                                                                   <  4:701132/149‑1 (MQ=255)

                                                              aaTAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATc                                                                                   >  2:147948/1‑149 (MQ=255)

                                                               aTAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATcc                                                                                  >  3:687608/1‑149 (MQ=255)

                                                                  aaaCACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGcc                                                                               >  1:100967/1‑149 (MQ=255)

                                                                      acTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCt                                                                                                                       >  4:491351/1‑105 (MQ=255)

                                                                      acTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCt                                                                                                                       <  3:491351/105‑1 (MQ=255)

                                                                      acTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGtt                                                                           >  2:41916/1‑149 (MQ=255)

                                                                         cacaACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCaa                                                                        <  3:69412/149‑1 (MQ=255)

                                                                          acaACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAAt                                                                       >  3:573695/1‑149 (MQ=255)

                                                                             aCCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAg                                                                    <  1:618334/149‑1 (MQ=255)

                                                                               ccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                            <  2:75133/123‑1 (MQ=255)

                                                                               ccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                            >  1:75133/1‑123 (MQ=255)

                                                                               ccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGc                                                                  >  2:731985/1‑149 (MQ=255)

                                                                                cAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCg                                                                 >  3:296481/1‑149 (MQ=255)

                                                                                 aaaaaTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGa                                                                >  3:380009/1‑149 (MQ=255)

                                                                                  aaaaTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGaa                                                               <  4:687608/149‑1 (MQ=255)

                                                                                   aaaTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAAt                                                              >  4:452792/1‑149 (MQ=255)

                                                                                       agagCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCCTCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAAt                                                          <  3:77494/149‑1 (MQ=255)

                                                                                        gagCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTGCAATCAGGCGAATAAAtt                                                         >  3:768847/1‑149 (MQ=255)

                                                                                               cGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCt                                                  >  2:285520/1‑149 (MQ=255)

                                                                                                ggCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGc                                                                  >  2:391284/1‑132 (MQ=255)

                                                                                                ggCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGc                                                                  <  1:391284/132‑1 (MQ=255)

                                                                                                ggCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTg                                                 >  4:690030/1‑149 (MQ=255)

                                                                                                       gagtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCt                                          >  4:649173/1‑149 (MQ=255)

                                                                                                              ttAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATc                                   <  2:707921/149‑1 (MQ=255)

                                                                                                              ttAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATc                                   <  3:683128/149‑1 (MQ=255)

                                                                                                                     catTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCt                            <  4:573695/149‑1 (MQ=255)

                                                                                                                       ttGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCttt                          >  2:864405/1‑149 (MQ=255)

                                                                                                                          ttaattaaTTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGc                       <  4:296481/149‑1 (MQ=255)

                                                                                                                             attaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCCCTGACTGATGTCAGTCACACAATCCGCCCTTTCAATCAGGCGAATAAATTCAGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctg                    >  3:83164/1‑149 (MQ=255)

                                                                                                                             attaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctg                    >  1:55696/1‑149 (MQ=255)

                                                                                                                                 attCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGa                <  1:562585/149‑1 (MQ=255)

                                                                                                                                  ttCGACCATTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAg               <  2:771263/149‑1 (MQ=255)

                                                                                                                                  ttCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAg               >  4:6557/1‑149 (MQ=255)

                                                                                                                                     gACCAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaaa                                                           >  2:740233/1‑102 (MQ=255)

                                                                                                                                     gACCAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaaa                                                           <  1:740233/102‑1 (MQ=255)

                                                                                                                                        cAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCtttt                         >  2:578454/1‑133 (MQ=255)

                                                                                                                                        cAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCtttt                         <  1:578454/133‑1 (MQ=255)

                                                                                                                                          gTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGt       >  3:242408/1‑149 (MQ=255)

                                                                                                                                             aGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTg    >  1:271743/1‑149 (MQ=255)

                                                                                                                                              gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGa   >  3:281740/1‑149 (MQ=255)

                                                                                                                                               cAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCt                                          >  3:725109/1‑109 (MQ=255)

                                                                                                                                               cAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCt                                          <  4:725109/109‑1 (MQ=255)

                                                                                                                                               cAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt  <  3:452792/149‑1 (MQ=255)

AGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAT  >  NC_000913/2383811‑2384102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A