Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F70 I0 R1 565 64.8 2371218 98.3% 2330907 140.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 850,998 G→A 11.8% intergenic (+33/+16) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,9980GA11.8% 189.6 / 14.5 68intergenic (+33/+16)ompX/opgEouter membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base G (27/33);  new base A (4/4);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

GGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCT  >  NC_000913/850851‑851141
                                                                                                                                                   |                                                                                                                                               
gggTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                                >  1:4325/1‑149 (MQ=255)
   tCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTCACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCg                                                                                                                                             >  1:589553/1‑149 (MQ=255)
      gCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAt                                                                                                                                          >  1:394899/1‑149 (MQ=255)
         gTTCAACCCGATCGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttt                                                                                                                                       <  1:471911/149‑1 (MQ=255)
               cccGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttatat                                                                                                                                 <  3:564861/149‑6 (MQ=255)
                 cGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTa                                                                                                                               >  2:145145/1‑149 (MQ=255)
                 cGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttatatca                                                                                                                               <  4:309209/149‑8 (MQ=39)
                  ggtGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGGCGGATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                                                                              <  2:404464/147‑1 (MQ=255)
                  gATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                                                                              <  4:464737/149‑1 (MQ=255)
                          aCGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCgg                                                                                                                      <  2:415194/149‑1 (MQ=255)
                          aCGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCgg                                                                                                                      <  2:387525/149‑1 (MQ=255)
                          aCGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCgg                                                                                                                      <  1:145145/149‑1 (MQ=255)
                            gTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGt                                                                                                                    >  3:282797/1‑149 (MQ=255)
                                     gACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAatat                                                                                                           >  1:486869/1‑149 (MQ=255)
                                     gACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAatat                                                                                                           >  1:254722/1‑149 (MQ=255)
                                     gACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAatat                                                                                                           >  2:238688/1‑149 (MQ=255)
                                       cTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCTGGTCGAAAata                                                                                                            <  4:347192/146‑1 (MQ=255)
                                       cTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCTGGTCGAAAata                                                                                                            >  3:347192/1‑146 (MQ=255)
                                       cTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCg                                                                                                         <  1:117548/149‑1 (MQ=255)
                                           tctTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTc                                                                                                     >  4:275251/1‑149 (MQ=255)
                                             ttACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                                >  4:192077/1‑104 (MQ=255)
                                             ttACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                                <  3:192077/104‑1 (MQ=255)
                                             ttACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCg                                                                                                   >  4:262320/1‑149 (MQ=255)
                                             ttACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttt                                                                                                                                       <  3:98899/113‑1 (MQ=255)
                                             ttACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttt                                                                                                                                       >  4:98899/1‑113 (MQ=255)
                                                cGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCgaga                                                                                                <  2:539526/149‑1 (MQ=255)
                                                   gCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATgg                                                                                             <  3:330429/149‑1 (MQ=255)
                                                          cGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAAt                                                                                      >  4:203084/1‑149 (MQ=255)
                                                            tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATcc                                                                                    >  3:204107/1‑149 (MQ=255)
                                                              ttCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCAc                                                                                  <  2:595853/149‑1 (MQ=255)
                                                                   aGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCt                                                                             <  2:503062/149‑1 (MQ=255)
                                                                        tGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAAc                                                                        <  2:4325/149‑1 (MQ=255)
                                                                        tGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAAc                                                                        <  4:95456/149‑1 (MQ=255)
                                                                            gTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGa                                                                    <  2:355098/149‑1 (MQ=255)
                                                                             tAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGaa                                                                   <  2:486869/149‑1 (MQ=255)
                                                                             tAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGaa                                                                   <  1:242317/149‑1 (MQ=255)
                                                                             tAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGaa                                                                   <  2:254722/149‑1 (MQ=255)
                                                                             tAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGaa                                                                   <  3:262320/149‑1 (MQ=255)
                                                                             tAGGCACCTGGATTGCCGGTGTTGGTCACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGaa                                                                   <  2:589553/149‑1 (MQ=255)
                                                                                  aCCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                                                              >  2:520482/1‑149 (MQ=255)
                                                                                   ccTGGATTGGCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                             <  1:103788/149‑1 (MQ=255)
                                                                                    cTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTg                                                            >  1:310621/1‑149 (MQ=255)
                                                                                        aTTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGt                                                        <  3:75013/149‑1 (MQ=255)
                                                                                             cGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGa                                                           >  2:454026/1‑141 (MQ=255)
                                                                                             cGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGa                                                           <  1:454026/141‑1 (MQ=255)
                                                                                             cGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATc                                                   <  2:578669/149‑1 (MQ=255)
                                                                                                 gtTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGcc                                               <  4:282797/149‑1 (MQ=255)
                                                                                                      ttACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCt                                          >  3:64844/1‑149 (MQ=255)
                                                                                                      ttACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCt                                          <  4:204107/149‑1 (MQ=255)
                                                                                                        aCCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCttt                                        >  3:297222/1‑149 (MQ=255)
                                                                                                              tCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTtctc                                  <  3:272506/149‑1 (MQ=255)
                                                                                                                tAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCgg                                            <  4:46018/137‑1 (MQ=255)
                                                                                                                tAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCgg                                            >  3:46018/1‑137 (MQ=255)
                                                                                                                   tCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCt                                                                             >  3:281712/1‑101 (MQ=255)
                                                                                                                   tCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCt                                                                             <  4:281712/101‑1 (MQ=255)
                                                                                                                   tCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGata                             >  2:55606/1‑149 (MQ=255)
                                                                                                                       tttGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATaaa                         <  2:38611/149‑1 (MQ=255)
                                                                                                                          ggTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACgg                      <  4:36032/149‑1 (MQ=255)
                                                                                                                             gATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTa                   <  1:238688/149‑1 (MQ=255)
                                                                                                                              aaaacaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAt                  >  2:128122/6‑149 (MQ=255)
                                                                                                                                 taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTg               >  1:371380/1‑149 (MQ=255)
                                                                                                                                  aaaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGa              <  3:129441/149‑1 (MQ=255)
                                                                                                                                   aaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGAt             >  4:317613/1‑149 (MQ=255)
                                                                                                                                   aaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGAt             >  4:330400/1‑149 (MQ=255)
                                                                                                                                    aaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATg            >  1:3578/1‑149 (MQ=255)
                                                                                                                                        tCCGCCTCTCGGCGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTGACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTaa        >  4:495175/1‑149 (MQ=255)
                                                                                                                                           gCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAt                  >  4:203556/1‑136 (MQ=255)
                                                                                                                                           gCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAt                  <  3:203556/136‑1 (MQ=255)
                                                                                                                                           gCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAAttt     >  4:218348/1‑149 (MQ=255)
                                                                                                                                            ccTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTc    >  1:97823/1‑149 (MQ=255)
                                                                                                                                              tctcGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCt  >  2:407352/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCT  >  NC_000913/850851‑851141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A