Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F182 I0 R1 19 76.1 1906635 87.9% 1675932 227.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.96.3% 340.9 / 3.8 82pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base . (36/43);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0009132,173,3620C.96.3% 341.4 / 4.5 82pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base . (36/43);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGC  >  NC_000913/2173088‑2173634
                                                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                                                
tttaaGCCACCATACCCCCCGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGTGGTGCAAACCAATAGTTTGCGTCGCGATCCACTGGGTAATGCTCATAATGATGACACCCGATATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATaccac                                                                                                                                                                                                                                                                                   <  2:48953/271‑1 (MQ=255)
 tGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGAAAAAAATCAGGCTTGCCGAAACCAccc                                                                                                                                                                                                                                                                                 >  4:155033/1‑275 (MQ=255)
     gCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCCCCTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATaccaccgccgt                                                                                                                                                                                                                                                                             >  2:92139/1‑270 (MQ=255)
            tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGTCCGTGGGGTTTGCAAACCAAGAGTTTGCGTCGCCATCCACAGGGTGATGCTCATAATGATTACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAAGCAAGCTTGCCGATACCA‑‑CCGCCGTATGTccc                                                                                                                                                                                                                                                                    <  2:38579/275‑1 (MQ=255)
                  cGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag                                                                                                                                                                                                                                                               <  2:533634/274‑1 (MQ=255)
                        gtcaCGCCCCAGCATTAGCCGCCTGTGGGGTGTNNNAACCAATAGTTTGGGTCGCGATCCCCAAGGTGATGCTCATAATGAGTACACCCTGGATTAAGTTGCGGAACAGATTTCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGTCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt                                                                                                                                                                                                                                                    <  3:212734/276‑1 (MQ=255)
                                   ggcttAGACGGCGGTTGGTTGGGCAAACCAGTAGTTAGCTTTGCGAGCCACGGGGTGAGGCTCATAATGATGACACCCGAGAGTCAGGTGCGGAATGGAGTGCCACGAGGCACGGCGACCGCCAGCGCCACGACGAAGACGATGGTGGCAAGATCGCCAAACGGCAGAACCTGATTACCCTGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                              <  4:347060/271‑1 (MQ=255)
                                    cATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                                                                                                                                                            <  2:155034/275‑1 (MQ=255)
                                         gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGTTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGACGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACC‑CCCCCCCGT‑TGTCCCAGCAGCCACGCCGGATCAAGGCCAATCa                                                                                                                                                                                                                                        >  2:520455/1‑274 (MQ=255)
                                              cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGCTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTAAG‑TGGGACAAAAATCAGGCTTGCCGATACCACCC‑‑CCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAc                                                                                                                                                                                                                                   >  4:472660/1‑274 (MQ=255)
                                                        tgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                                                                                                                                                                         <  2:474598/274‑1 (MQ=255)
                                                               cAATAGTGTGGTTCGCGATCCACAGTGATCAGCACATATGGATGACACCCTAGCGTAAGGAGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAGTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTCGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                                                                                                                                                                  <  4:561919/274‑1 (MQ=255)
                                                                       tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCCCCGAACTTCGCCTGTa                                                                                                                                                                                                          >  2:125056/1‑274 (MQ=255)
                                                                       tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGACGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaa                                                                                                                                                                                                         >  4:157033/1‑275 (MQ=255)
                                                                       tGCATCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                                                                         <  1:49056/227‑1 (MQ=255)
                                                                         cGTCGGGTTCCACAGGGTGATGCGCATAATGAGGAAACCCGAGATGAAGGTGCGGAACAGAGTTCCACGTGGGACGGCGACAGCCATCTCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACAGCAGCAACTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaaa                                                                                                                                                                                                        <  4:601006/274‑1 (MQ=255)
                                                                             gcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                                                                                                                                                                   <  2:157034/275‑1 (MQ=255)
                                                                              cgATCAAAAGGGTGATGCTCATAATGAGGACAACCGACATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                                                                                                                                                                  <  4:125056/275‑1 (MQ=255)
                                                                                           gATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCAACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                 <  1:2595/279‑1 (MQ=255)
                                                                                            aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTTAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACCCCTGACCGCCGAACTTCGCCTGTAAACGACCACCACCCTGCTTaa                                                                                                                                                                                     >  4:547410/1‑273 (MQ=255)
                                                                                             tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGTTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATACAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg                                                                                                                                                                                   >  2:603217/1‑275 (MQ=255)
                                                                                             tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCCCACAAACAGAAATTAAAATGGTTAG‑TGGGATAAAAATCAGGCTTCCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCAAACTTCCCCTGTAAACGACTTAGAGCCTGATTAGcc                                                                                                                                                                                   >  4:222972/1‑274 (MQ=255)
                                                                                               cTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                 <  2:627174/275‑1 (MQ=255)
                                                                                                tCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                 <  2:547411/274‑1 (MQ=255)
                                                                                                  aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAg                                                                                                                                                                                     >  2:102449/1‑268 (MQ=255)
                                                                                                  aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                                                                                                                                                               >  4:418835/1‑274 (MQ=255)
                                                                                                    aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCg                                                                                                                                                                                                                                                       >  4:599566/1‑200 (MQ=255)
                                                                                                    aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCaa                                                                                                                                                                                                                                           >  1:196230/1‑212 (MQ=255)
                                                                                                    aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAc                                                                                                                                                                                                                                   >  4:288738/1‑220 (MQ=255)
                                                                                                    aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                                                                                                                                                            >  2:196371/1‑275 (MQ=255)
                                                                                                      tgatgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGAGCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATaccaccg                                                                                                                                                                                                                                                                                 <  4:717419/174‑2 (MQ=255)
                                                                                                        atggaacCCGGGAGGAAGGTGAGGACAAGAATTCCACGAGGCACGGCGACACCCATGGCCAGGAAGAAGCCAATGGTGGCAAGGTCGGCAAACGGCAGCAACGGATTACCCGGGACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAAGCAGGCTTGCCGAGACCA‑‑CCGCCGGATGTCCCAGCAGCAACGACGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTa                                                                                                                                                                         <  4:520454/269‑1 (MQ=255)
                                                                                                        atgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                                                                                                                                                                   <  2:599567/200‑1 (MQ=255)
                                                                                                        atgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                                                                                                                                                               <  2:288739/220‑1 (MQ=255)
                                                                                                         tgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCCCCGAACTTACCCTGAAAAAGACTCCGACCCTGCTTAGCCAAGGCCGTTaaa                                                                                                                                                                       >  4:474168/1‑275 (MQ=255)
                                                                                                            cacCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                                                                                                                                                           >  2:329486/1‑236 (MQ=255)
                                                                                                                cGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc                                                                                                                                                                                                       <  4:329485/236‑1 (MQ=255)
                                                                                                                             cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                                                   >  2:29384/1‑275 (MQ=255)
                                                                                                                             cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                                                   >  2:11685/1‑275 (MQ=255)
                                                                                                                             cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc                                                                                                                                               >  1:155939/1‑279 (MQ=255)
                                                                                                                                          cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGCTTTAATCACCCGTGGCAt                                                                                                                                       >  2:194624/1‑274 (MQ=255)
                                                                                                                                             gATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTag                                                                                                                                    <  2:403699/274‑1 (MQ=255)
                                                                                                                                             gATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTag                                                                                                                                    <  2:195765/274‑1 (MQ=255)
                                                                                                                                               tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTCGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagca                                                                                                                                  >  4:398687/1‑274 (MQ=255)
                                                                                                                                                                cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                                                                         >  1:181708/1‑138 (MQ=255)
                                                                                                                                                                cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                                                                         <  3:181708/138‑1 (MQ=255)
                                                                                                                                                                        agaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                        <  2:222973/275‑1 (MQ=255)
                                                                                                                                                                           agcgcatgcgggCAAGACTGCCAAAAGGCAGGACCTGATTAACCGGAAACCAAACAGCAATTAAAATGTTGAG‑GGGGATAAAAATCAAGCTTGCCGATACCA‑‑CCACCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTGCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGCTGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                                                      <  4:92139/264‑1 (MQ=255)
                                                                                                                                                                           agCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGc                                                                                                     <  2:158681/275‑1 (MQ=255)
                                                                                                                                                                           agCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGc                                                                                                     <  2:418836/275‑1 (MQ=255)
                                                                                                                                                                              cGATGGACGCAAGATCGCCAAACGGAAGCAACTGATCACCCGGAACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTGGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                                                              <  1:27142/279‑1 (MQ=255)
                                                                                                                                                                                 tggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGATTTACTCACCCGTGGCATTAGCACCANNNCTCCCGCCCTTTTTTCCCCCCGCTCCaaaca                                                                                             >  3:209964/1‑274 (MQ=255)
                                                                                                                                                                                    tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                                                                                                                                                             >  4:432835/1‑210 (MQ=255)
                                                                                                                                                                                        aaGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATggg                                                                                                                                                         <  2:432836/210‑1 (MQ=255)
                                                                                                                                                                                                  aaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCAGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                                               <  2:274961/274‑1 (MQ=255)
                                                                                                                                                                                                       cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGc                                                                                                  >  4:77771/1‑250 (MQ=255)
                                                                                                                                                                                                           aCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                                                              <  2:77771/250‑1 (MQ=255)
                                                                                                                                                                                                               caccaCCCGGCACCCAAACACCAATTAAAA‑GGTGGGTTGGGTTAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCNAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                        <  3:140052/232‑1 (MQ=255)
                                                                                                                                                                                                                  tacacagccaacaAATAGCCATGAGAAGGGTGAG‑GCGGATAGAAATAAGGCTAGCCTATACCA‑‑CCGCCGGATGTCCCTCAAGCGACGCCGAAGCAAGGCCCAGCAAGAACTCCTGACCGACGAACTTCGCCTGTAAACAACGAAGAGCCTGTTTAGCGATGGGCGTTATACCATCCTTGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGACGACGTTTTTACCGCCTGCTGCAATACACCTGTGACATCGTAACCCGCGAGGATGCCg                                                              <  4:204771/265‑1 (MQ=255)
                                                                                                                                                                                                                  tACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                                              <  2:474169/275‑1 (MQ=255)
                                                                                                                                                                                                                     ccGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgcg                                                                       >  4:431746/1‑263 (MQ=255)
                                                                                                                                                                                                                      aggCACAAAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGACGATACCA‑‑CCGGCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGGAAACGACTACGAGCCTGCTTAGCGATGCGCGTTAAACCAGCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAg                                                      <  1:155222/278‑1 (MQ=255)
                                                                                                                                                                                                                       ggCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCAc                                                                                                                           >  2:570917/1‑209 (MQ=255)
                                                                                                                                                                                                                       ggCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATCAt                                                         >  2:258256/1‑275 (MQ=255)
                                                                                                                                                                                                                       ggCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCGGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGGTTAATCACCCGTTGCATTAGCAGCAGCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGAAATCGTAACCCGCGAGGATGCCGATatg                                                         >  4:246279/1‑273 (MQ=255)
                                                                                                                                                                                                                        gCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATaccac                                                                                                                                                                                                                                                                                   <  4:338188/58‑1 (MQ=255)
                                                                                                                                                                                                                         cacacaAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGa                                                                   <  2:431747/263‑1 (MQ=255)
                                                                                                                                                                                                                         cacacaAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                                        >  2:635520/1‑274 (MQ=255)
                                                                                                                                                                                                                          acacaAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCGCTGCCGCCGGTTTTACCGCCAGCTGCAATACACCGTTGACATCGTAACCCACGTGGATGCCGGTACTCAcccc                                                   >  1:203808/1‑278 (MQ=255)
                                                                                                                                                                                                                           cacaAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgcc                                                                                                                       <  4:570916/209‑1 (MQ=255)
                                                                                                                                                                                                                               atcAGCAAATAAAATGGGGTG‑GTGGAGAAAAAACAGGCCTTCCGTTACCA‑‑CCGCCGTAGGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGTAACGCCTGACCGCCGAACTTCGCCTGTAAACGGCTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGGTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCa                                                 <  4:11685/273‑1 (MQ=255)
                                                                                                                                                                                                                                   gCAATTAAAATGGTGAG‑TGGGATAANAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat                                                                                                                                                            <  3:139860/164‑1 (MQ=255)
                                                                                                                                                                                                                                   gCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat                                                                                                                                                            >  1:139860/1‑164 (MQ=255)
                                                                                                                                                                                                                                      aTTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaa                                          >  2:272477/1‑275 (MQ=255)
                                                                                                                                                                                                                                       ttAAAATGGTGAG‑TGGGCTAAAAATCAGGCTGTCCGGTACCA‑‑CCGCCTTATGTCCCAGCAGTAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGTGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaaa                                         <  2:659976/275‑1 (MQ=255)
                                                                                                                                                                                                                                        tAAAATGGGGAG‑TGGGGTAAAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaaa                                         <  2:246280/274‑1 (MQ=255)
                                                                                                                                                                                                                                            aTGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCNTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTCCCTCCGTTTTTCCCGCCAGCTGCNATACACCTTTCACACCGTAACCCGCGCGTANNCCAAACATCAGTCCCATcaaccaaccccccc                                  >  3:87442/1‑265 (MQ=255)
                                                                                                                                                                                                                                            aTGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGt                                                                                                                                            >  4:710510/1‑171 (MQ=255)
                                                                                                                                                                                                                                              ggTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                                                                  >  4:635081/1‑163 (MQ=255)
                                                                                                                                                                                                                                                tGAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCa                                                                                                                                        <  2:710511/171‑1 (MQ=255)
                                                                                                                                                                                                                                                 gAG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAAAACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGa                               >  2:448445/1‑275 (MQ=255)
                                                                                                                                                                                                                                                  aG‑TGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                                                              <  2:635082/163‑1 (MQ=255)
                                                                                                                                                                                                                                                   g‑TGGGATAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGa                               <  2:188117/273‑1 (MQ=255)
                                                                                                                                                                                                                                                          tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc                                                                                                                                         <  3:108863/161‑1 (MQ=255)
                                                                                                                                                                                                                                                          tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc                                                                                                                                         >  1:108863/1‑161 (MQ=255)
                                                                                                                                                                                                                                                                tCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCaaa                  <  2:472661/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCAAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCaa          >  2:567483/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                 cccccGCCGTATGTCCCAGCCGAAAGGCCGGGTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAAAACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgcgc  <  4:448444/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                                                
TTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGC  >  NC_000913/2173088‑2173634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A