Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F187 I0 R2 632 116.8 4275814 98.0% 4190297 140.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,357:1 +T 5.3% intergenic (+151/+21) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3571.T 5.3% 316.3 / 12.9 75intergenic (+151/+21)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base . (35/36);  new base T (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

GCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATC  >  NC_000913/1446209‑1446500
                                                                                                                                                     |                                                                                                                                               
gCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                                                                  >  2:557558/1‑149 (MQ=255)
   aaaCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑Gt                                                                                                                                               >  3:989766/1‑148 (MQ=255)
    aaCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTAc                                                                                                                                             <  2:863718/149‑1 (MQ=255)
     aCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACa                                                                                                                                            <  2:791061/149‑1 (MQ=255)
      ccGCAATATCGGGACAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACAt                                                                                                                                           >  1:807465/1‑149 (MQ=255)
        gCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACAtac                                                                                                                                         <  1:629380/149‑3 (MQ=255)
           atatCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCaa                                                                                                                                       <  3:625820/148‑1 (MQ=255)
             atCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACg                                                                                                                                     <  3:710881/148‑1 (MQ=255)
                  gATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAt                                                                                                                                <  4:202802/148‑1 (MQ=255)
                   aTAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAtt                                                                                                                               <  3:599171/148‑1 (MQ=255)
                       cGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAttagtt                                                                                                                           <  3:988773/148‑1 (MQ=255)
                            cAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGa                                                                                                                     >  2:202176/1‑149 (MQ=255)
                             aGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGc                                                                                                                                    >  1:651083/1‑133 (MQ=255)
                             aGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGc                                                                                                                                    <  2:651083/133‑1 (MQ=255)
                             aGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGc                                                                                                                                    <  2:550167/133‑1 (MQ=255)
                             aGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTAAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGc                                                                                                                                    >  1:550167/1‑133 (MQ=255)
                              ggAGAAGGCGATTGATCTATTTTCCTGGAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGaa                                                                                                                    <  3:728451/148‑1 (MQ=255)
                                agaAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAAtt                                                                                                                  >  4:754155/1‑148 (MQ=255)
                                    ggCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                                                              <  4:989766/148‑1 (MQ=255)
                                      cGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                                                            >  4:1003052/1‑148 (MQ=255)
                                            aTCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAtt                                                                                                                               <  4:945517/123‑1 (MQ=255)
                                            aTCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAtt                                                                                                                               >  3:945517/1‑123 (MQ=255)
                                             tCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTc                                                                                                     >  4:396069/1‑148 (MQ=255)
                                                 ttttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAttagtta                                                                                                                          >  2:261002/1‑123 (MQ=255)
                                                 ttttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAttagtta                                                                                                                          <  1:261002/123‑1 (MQ=255)
                                                  tttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTg                                                                                                >  4:709794/1‑148 (MQ=255)
                                                       tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCa                                                                                          >  1:371538/1‑149 (MQ=255)
                                                         aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCAt                                                                                         >  3:1020650/1‑148 (MQ=255)
                                                              aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCaa                                                                                    >  4:143110/1‑148 (MQ=255)
                                                                gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                                                                 >  1:955744/1‑149 (MQ=255)
                                                                 tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGTCTTCATCCCAAAGc                                                                                 >  4:561187/1‑148 (MQ=255)
                                                                  gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                                                                >  3:437866/1‑148 (MQ=255)
                                                                    atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTg                                                                             <  2:174988/149‑1 (MQ=255)
                                                                       ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                                                           <  4:164835/148‑1 (MQ=255)
                                                                        tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCtt                                                                          >  3:12187/1‑148 (MQ=255)
                                                                          aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAGTATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                                                                       <  2:713872/149‑1 (MQ=255)
                                                                          aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCaaa                                                                                   >  1:184090/1‑137 (MQ=255)
                                                                          aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCaaa                                                                                   <  2:184090/137‑1 (MQ=255)
                                                                          aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaa                                                                        <  4:285563/148‑1 (MQ=255)
                                                                          aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaa                                                                        >  3:907595/1‑148 (MQ=255)
                                                                                tCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg                                                                  <  4:437866/148‑1 (MQ=255)
                                                                                tCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGt                                                                 >  2:327841/1‑149 (MQ=255)
                                                                                 ccTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGtt                                                                <  2:371538/149‑1 (MQ=255)
                                                                                 ccTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGt                                                                 >  3:183539/1‑148 (MQ=255)
                                                                                          ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                                                       <  1:327841/149‑1 (MQ=255)
                                                                                          ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                                                       <  1:472339/149‑1 (MQ=255)
                                                                                            gCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc                                                      <  3:754155/148‑1 (MQ=255)
                                                                                               ttttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTa                                                                                                                <  4:197146/87‑1 (MQ=255)
                                                                                               ttttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTa                                                                                                                >  3:197146/1‑87 (MQ=255)
                                                                                               ttttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActct                                                   <  4:565797/148‑1 (MQ=255)
                                                                                               ttttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTg                                                  >  2:194602/1‑149 (MQ=255)
                                                                                                tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                                                        >  4:992811/1‑142 (MQ=255)
                                                                                                tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                                                        <  3:992811/142‑1 (MQ=255)
                                                                                                       tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt                                                                              <  4:1028454/113‑1 (MQ=255)
                                                                                                       tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt                                                                              >  3:1028454/1‑113 (MQ=255)
                                                                                                        gAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaa                                          >  3:715831/1‑148 (MQ=255)
                                                                                                          aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATc                                       <  1:202176/149‑1 (MQ=255)
                                                                                                            aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGa                                                                                                                     >  3:524593/1‑69 (MQ=255)
                                                                                                            aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGa                                                                                                                     <  4:524593/69‑1 (MQ=255)
                                                                                                                  tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGa                               >  2:64792/1‑149 (MQ=255)
                                                                                                                  tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATGTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATg                                >  4:732415/1‑148 (MQ=255)
                                                                                                                     tCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAg                            >  1:724732/1‑149 (MQ=255)
                                                                                                                        aaTATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGcc                          >  3:563144/1‑148 (MQ=255)
                                                                                                                         atatCTGTTTTAACTAATTGGCGTTGAA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCAACCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGa                        <  2:348980/149‑1 (MQ=255)
                                                                                                                          tatCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGa                        <  3:143110/148‑1 (MQ=255)
                                                                                                                               gTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcct                   >  3:321823/1‑148 (MQ=255)
                                                                                                                                ttttAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCCGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcctg                  >  3:573795/1‑148 (MQ=255)
                                                                                                                                      cTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCa                                   <  3:872486/125‑1 (MQ=255)
                                                                                                                                      cTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCa                                   >  4:872486/1‑125 (MQ=255)
                                                                                                                                        aaTTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtttt          <  4:12187/148‑1 (MQ=255)
                                                                                                                                         aTTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGttttt         <  3:732415/148‑1 (MQ=255)
                                                                                                                                           tGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCCGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtca       <  4:573795/148‑1 (MQ=255)
                                                                                                                                            ggCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcatc     <  1:557558/149‑1 (MQ=255)
                                                                                                                                             gCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcatca    >  1:820933/1‑149 (MQ=255)
                                                                                                                                             gCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcatc     >  3:336166/1‑148 (MQ=255)
                                                                                                                                                ttGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCatcatc  <  4:183539/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATC  >  NC_000913/1446209‑1446500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A