Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F25 I0 R2 821 102.6 3645220 97.9% 3568670 140.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,946,035 G→T 7.7% intergenic (‑59/+80) ruvA ← / ← yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,946,0350GT 7.7% 174.2 / 5.4 52intergenic (‑59/+80)ruvA/yobIcomponent of RuvABC resolvasome, regulatory subunit/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (20/28);  new base T (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTG  >  NC_000913/1945896‑1946182
                                                                                                                                           |                                                                                                                                                   
ttCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGc                                                                                                                                            >  4:674658/1‑149 (MQ=255)
 tCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGc                                                                                                                                            >  1:887689/1‑148 (MQ=255)
    tAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGaa                                                                                                                                         >  2:858091/1‑148 (MQ=255)
      gCCTACGCCGCCCACTTCAATTAACTCCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGa                                                                                                                                      <  3:590638/149‑1 (MQ=255)
       ccTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGTTATCCAGCGAAGGAt                                                                                                                                     <  4:670016/149‑1 (MQ=255)
          aCGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATgaa                                                                                                                                  >  3:707180/1‑149 (MQ=255)
           cgccgcCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATgaa                                                                                                                                  <  1:858091/148‑1 (MQ=255)
                    cTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGc                                                                                                                        <  4:769161/149‑1 (MQ=255)
                     ttCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCg                                                                                                                       >  3:383299/1‑149 (MQ=255)
                          ttAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGGCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgt                                                                                                                   >  1:590099/1‑148 (MQ=255)
                                ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                                                                             <  1:150989/148‑1 (MQ=255)
                                ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                                                                             >  2:150989/1‑148 (MQ=255)
                                ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGa                                                                                                            <  3:674658/149‑1 (MQ=255)
                                 cAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGAt                                                                                                           <  4:883673/149‑1 (MQ=255)
                                 cAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGAt                                                                                                           <  3:797559/149‑1 (MQ=255)
                                 cAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGAt                                                                                                           <  4:48249/149‑1 (MQ=255)
                                     gggggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAAc                                                                                                       <  3:288071/149‑1 (MQ=255)
                                          tggttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAACGCTGTATGATAAAAAAATGCTGGATAGATATCTAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCAATGAATTGaaa                                                                                                  >  4:350125/3‑149 (MQ=255)
                                             tttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaaaaa                                                                                                <  2:590099/148‑1 (MQ=255)
                                              ttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAAt                                                                                               <  1:516061/148‑1 (MQ=255)
                                              ttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATg                                                                                              <  3:24161/149‑1 (MQ=255)
                                                 tCAATGATGATGCCGCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                                                           <  3:322000/149‑1 (MQ=255)
                                                     tgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAg                                                                                        >  1:247987/1‑148 (MQ=255)
                                                        tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                                                                                     >  2:32082/1‑148 (MQ=255)
                                                         gatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATaa                                                                                   <  4:707180/149‑1 (MQ=255)
                                                              ctctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAg                                                                                                                                        >  3:165244/1‑91 (MQ=255)
                                                              ctctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAg                                                                                                                                        <  4:165244/91‑1 (MQ=255)
                                                              atctAAGTTTGCCTATCACAAAAAGCTCCTGCATAATGAATCAAAGAAAATGCTGGATGATAAAAAAATGCTGGATAGATATCCAGAGAAGGATGAAGATAACTTGCGAGGTGTCACGATGAACCGAAAAATGGCACATTATAATTTAt                                                                              <  4:383299/148‑1 (MQ=255)
                                                                ctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATCAACTGAAAAATGGCACAGTATAATTTAtt                                                                             <  1:445974/148‑1 (MQ=255)
                                                                     tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                                                           >  4:842690/1‑129 (MQ=255)
                                                                     tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                                                           <  3:842690/129‑1 (MQ=255)
                                                                          cTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGAt                                                                                                                                     >  2:82578/1‑82 (MQ=255)
                                                                          cTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGAt                                                                                                                                     <  1:82578/82‑1 (MQ=255)
                                                                                  tGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGaa                                                           >  2:248238/1‑148 (MQ=255)
                                                                                   gACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                                             <  4:800354/113‑1 (MQ=255)
                                                                                   gACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                                             >  3:800354/1‑113 (MQ=255)
                                                                                         ccTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAat                                                     >  2:861103/1‑147 (MQ=255)
                                                                                         ccTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAat                                                     <  1:861103/147‑1 (MQ=255)
                                                                                           tGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAttt                                                 <  4:849664/149‑1 (MQ=255)
                                                                                                     aTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTaa                                              <  3:83237/142‑1 (MQ=255)
                                                                                                     aTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTaa                                              >  4:83237/1‑142 (MQ=255)
                                                                                                       cAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACata                                     >  4:558650/1‑149 (MQ=255)
                                                                                                         aaGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACatat                                    >  2:323567/1‑148 (MQ=255)
                                                                                                                   tGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTgaagaa                         >  4:323650/1‑149 (MQ=255)
                                                                                                                       tGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGc                     <  3:550944/149‑1 (MQ=255)
                                                                                                                        gATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGc                     <  1:32082/148‑1 (MQ=255)
                                                                                                                                 tcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTatat                                                                >  2:533055/3‑96 (MQ=255)
                                                                                                                                 tcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTatat                                                                <  1:533055/94‑1 (MQ=255)
                                                                                                                                   gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAg                                >  1:521672/1‑126 (MQ=255)
                                                                                                                                   gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAg                                <  2:521672/126‑1 (MQ=255)
                                                                                                                                    cTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAata         <  2:247987/148‑1 (MQ=255)
                                                                                                                                     tGGATAGATAGCCAGAGAAGGATTAAGAAGACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATTCGTAATAAAAAtata       <  3:350125/149‑1 (MQ=255)
                                                                                                                                          agataTCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTACAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTg  >  3:449026/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
TTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTG  >  NC_000913/1945896‑1946182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A