Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F25 I0 R2 821 102.6 3645220 97.9% 3568670 140.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,074,810 Δ1 bp 5.6% coding (32/1533 nt) yeeR → CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,8100T. 5.6% 446.4 / 17.8 125coding (32/1533 nt)yeeRCP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (69/49);  new base . (5/2);  total (74/51)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 132 total aligned reads displayed.

AACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCATATT  >  NC_000913/2074662‑2074958
                                                                                                                                                    |                                                                                                                                                     
aaCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                                                                                                                                                       >  4:266946/1‑149 (MQ=255)
   cATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTACTGAt                                                                                                                                                    >  3:149415/1‑149 (MQ=255)
      cGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑g                                                                                                                                                 >  2:616342/1‑148 (MQ=255)
      cGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑g                                                                                                                                                 >  2:853604/1‑148 (MQ=255)
         ctctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACTGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCatg                                                                                                                                             >  3:874139/1‑147 (MQ=255)
         ctctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAg                                                                                                                                              >  1:60735/1‑148 (MQ=255)
           ctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGa                                                                                                                                            >  1:216604/1‑148 (MQ=255)
            tctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGAtt                                                                                                                                          >  4:845000/1‑149 (MQ=255)
             ctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGAtt                                                                                                                                          <  2:151780/148‑1 (MQ=255)
                   tCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGa                                                                                                                                            <  4:400781/140‑1 (MQ=255)
                     ccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttct                                                                                                                                 >  3:496415/1‑149 (MQ=255)
                     ccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttct                                                                                                                                 >  3:446243/1‑149 (MQ=255)
                      cGGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttct                                                                                                                                 >  2:708013/1‑148 (MQ=255)
                         tcatgaTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCg                                                                                                                              <  1:708013/148‑1 (MQ=255)
                         tcatcaTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGc                                                                                                                             <  4:446243/149‑1 (MQ=255)
                            tcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCtt                                                                                                                          <  4:660309/149‑1 (MQ=255)
                              atGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCttt                                                                                                                         <  1:410293/148‑1 (MQ=255)
                                      gACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCACTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCaga                                                                                                                 >  1:569153/1‑148 (MQ=255)
                                       tcccGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTAGTTCAGAGc                                                                                                               <  4:779503/148‑1 (MQ=255)
                                         ccGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCAt                                                                                                             >  4:32513/1‑149 (MQ=255)
                                         ccGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCAt                                                                                                             >  3:250504/1‑149 (MQ=255)
                                           ggACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTa                                                                                                           >  4:750268/1‑149 (MQ=255)
                                            gACCGGCGCAACAGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTa                                                                                                           <  1:837864/148‑1 (MQ=255)
                                             aCCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAAt                                                                                                         <  3:266946/149‑1 (MQ=255)
                                             aCCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAAt                                                                                                         <  4:250504/149‑1 (MQ=255)
                                               cGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATc                                                                                                        >  1:822552/1‑148 (MQ=255)
                                                    cAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTg                                                                                                                                        >  2:91251/1‑111 (MQ=255)
                                                    cAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTg                                                                                                                                        <  1:91251/111‑1 (MQ=255)
                                                      aCGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACgg                                                                                                 <  2:216604/148‑1 (MQ=255)
                                                         gATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttcttc                                                                                                                               >  4:399853/1‑115 (MQ=255)
                                                         gATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttcttc                                                                                                                               <  3:399853/115‑1 (MQ=255)
                                                          aTCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTc                                                                                             <  2:311199/148‑1 (MQ=255)
                                                          aTCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCt                                                                                            >  3:197277/1‑149 (MQ=255)
                                                             ttCAACGCCACATTCGCTGGCATTAACAGTAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTgcg                                                                                         >  3:777248/1‑149 (MQ=255)
                                                             ttCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTgcg                                                                                         >  4:865508/1‑149 (MQ=255)
                                                              tCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTgcgc                                                                                        <  3:598005/149‑1 (MQ=255)
                                                               cAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTgcgc                                                                                        >  2:503822/1‑148 (MQ=255)
                                                               cAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTgcgc                                                                                        >  2:22859/1‑148 (MQ=255)
                                                                aaCGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTg                                                                                      >  3:806372/1‑149 (MQ=255)
                                                                 aCGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTg                                                                                      >  1:537025/1‑148 (MQ=255)
                                                                  cGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAAt                                                                                                         >  1:884884/1‑128 (MQ=255)
                                                                  cGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAAt                                                                                                         <  2:884884/128‑1 (MQ=255)
                                                                     cacaTTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAgg                                                                                 >  3:253532/1‑149 (MQ=255)
                                                                       caTTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAggg                                                                                <  1:616342/148‑1 (MQ=255)
                                                                       caTTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGc                                                                               <  3:714443/149‑1 (MQ=255)
                                                                        aTTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCt                                                                              <  4:874139/149‑1 (MQ=255)
                                                                         ttCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCt                                                                              >  2:13261/1‑148 (MQ=255)
                                                                              tGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATAtt                                                                        >  3:756118/1‑149 (MQ=255)
                                                                                 cATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGc                                                                      <  2:822552/148‑1 (MQ=255)
                                                                                 cATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGACATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCt                                                                     <  3:561712/149‑1 (MQ=255)
                                                                                 cATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTACTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCt                                                                     <  4:149415/149‑1 (MQ=255)
                                                                                  aTTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCt                                                                              >  3:266849/1‑139 (MQ=255)
                                                                                   ttAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtgt                                                                   >  3:589085/1‑149 (MQ=255)
                                                                                   ttAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtgt                                                                   >  4:274492/1‑149 (MQ=255)
                                                                                   ttAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtgt                                                                   >  3:369419/1‑149 (MQ=255)
                                                                                   ttAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtg                                                                    >  1:70580/1‑148 (MQ=255)
                                                                                   ttAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGC‑GATCAGCAGGAt                                                                                                                                           <  1:54124/77‑1 (MQ=255)
                                                                                    tAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtgt                                                                   >  1:491343/1‑148 (MQ=255)
                                                                                    tAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtgt                                                                   >  1:323003/1‑148 (MQ=255)
                                                                                      aCAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTc                                                                >  4:638093/1‑149 (MQ=255)
                                                                                         aTAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGc                                                                      >  2:433573/1‑140 (MQ=255)
                                                                                           aaCATGATATTCATCACAGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTc                                                           >  4:107068/1‑149 (MQ=255)
                                                                                             cATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCa                                                                                                                   >  4:430762/1‑91 (MQ=255)
                                                                                             cATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCa                                                                                                                   <  3:430762/91‑1 (MQ=255)
                                                                                             cATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCt                                                               >  1:263369/1‑143 (MQ=255)
                                                                                              aTGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTc                                                                                             >  1:682785/1‑112 (MQ=255)
                                                                                              aTGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTc                                                                                             <  2:682785/112‑1 (MQ=255)
                                                                                               tGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTcggc                                                        <  2:318720/148‑1 (MQ=255)
                                                                                               tGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTcggc                                                        >  2:837590/1‑148 (MQ=255)
                                                                                                gATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTcggcc                                                       >  1:804602/1‑148 (MQ=255)
                                                                                                  tatTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtgt                                                                   <  4:472132/134‑1 (MQ=255)
                                                                                                  tatTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCtgt                                                                   >  3:472132/1‑134 (MQ=255)
                                                                                                       atcaCGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGa                                                                                                                                                     <  3:357602/48‑1 (MQ=255)
                                                                                                       atcaCGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTa                                               <  3:84668/149‑1 (MQ=255)
                                                                                                                tGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTTCTGTGTCTGTTcggcccggc                                                   <  4:756402/136‑1 (MQ=255)
                                                                                                                 gACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTggctggct                                     >  4:657054/1‑149 (MQ=255)
                                                                                                                  aCTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCta                                    >  4:775952/1‑149 (MQ=255)
                                                                                                                    tATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTcggcc                                                       <  3:807754/128‑1 (MQ=255)
                                                                                                                    tATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTcggcc                                                       >  4:807754/1‑128 (MQ=255)
                                                                                                                     aTGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTc                                                                                             <  2:30594/89‑1 (MQ=255)
                                                                                                                     aTGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTc                                                                                             >  1:30594/1‑89 (MQ=255)
                                                                                                                     aTGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCtata                                  >  1:810412/1‑148 (MQ=255)
                                                                                                                             gATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGaa                          <  2:913029/148‑1 (MQ=255)
                                                                                                                              aTAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAAc                         >  1:691182/1‑148 (MQ=255)
                                                                                                                              aTAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACg                        >  4:866958/1‑149 (MQ=255)
                                                                                                                                aGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGc                       >  1:674674/1‑148 (MQ=255)
                                                                                                                                   cGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCACCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATa                    >  2:323127/1‑148 (MQ=255)
                                                                                                                                     gcgcgcTGATCCTGCTGATC‑GCAGGATTTGCAATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAAc                  <  1:429630/148‑1 (MQ=255)
                                                                                                                                          cTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGgt             >  1:863352/1‑148 (MQ=255)
                                                                                                                                           aaaTCCTGC‑GATCAGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGgttg           >  3:416535/3‑149 (MQ=255)
                                                                                                                                           aaaTCCTGC‑GATCAGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGgttg           >  3:493703/3‑149 (MQ=255)
                                                                                                                                            aaTCCTGC‑GATCAGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGgttgt          >  4:369059/2‑149 (MQ=255)
                                                                                                                                             aTCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGgttgtt         <  3:750268/149‑1 (MQ=255)
                                                                                                                                             aTCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGgttgt          <  2:60735/148‑1 (MQ=255)
                                                                                                                                             aTCCTGC‑GATCAGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGgt             <  2:696686/145‑1 (MQ=255)
                                                                                                                                               ccTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTcc       <  4:253532/149‑1 (MQ=255)
                                                                                                                                                  gctgATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGc                                                                      <  2:549376/83‑1 (MQ=255)
                                                                                                                                                  gctgATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCata    <  3:866958/149‑1 (MQ=255)
                                                                                                                                                  gctgATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCat     >  1:199556/1‑148 (MQ=255)
                                                                                                                                                    tgATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCATAtt  >  3:194011/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCATATT  >  NC_000913/2074662‑2074958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A