Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A26 F25 I0 R2
|
821 |
102.6 |
3645220 |
97.9% |
3568670 |
140.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,291,284 |
C→A |
14.3% |
intergenic (+137/+74) |
narP → / ← ccmH |
response regulator in two‑component regulatory system with NarQ/heme lyase, CcmH subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,291,284 | 0 | C | A | 14.3%
| 108.7
/ 9.5
| 35 | intergenic (+137/+74) | narP/ccmH | response regulator in two‑component regulatory system with NarQ/heme lyase, CcmH subunit |
| Reads supporting (aligned to +/- strand): ref base C (19/11); new base A (2/3); total (21/14) |
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 |
AAATAGCCCGATGGATTTATCATCGGGCTGAGATTTATGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCAC > NC_000913/2291148‑2291417
|
aaaTAGCCCGATAGATGTATCATCGAGTTGAGAATTATGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGGTCAATTTATTGAATTTACACAATTTTCTAGGCCGGATGCGGCGTGAACGCCTTATCCg < 4:15148/149‑1 (MQ=18)
gATGGATTTATCATCGGGCTGAGATTTATGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACt > 4:703258/1‑149 (MQ=255)
agaTTTATGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGt < 1:68249/148‑1 (MQ=255)
aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGAt < 4:841677/149‑1 (MQ=255)
gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGAt > 4:419390/1‑143 (MQ=255)
gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGAt < 3:419390/143‑1 (MQ=255)
gcTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCtttt > 4:735674/1‑149 (MQ=255)
cTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCtttt > 1:3343/1‑148 (MQ=255)
cTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCtttt > 2:3150/1‑148 (MQ=255)
tACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCttttt > 2:524667/1‑148 (MQ=255)
aCGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCtttt > 4:332926/1‑146 (MQ=255)
aCGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCtttt < 3:332926/146‑1 (MQ=255)
aCGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTtttat < 3:114529/149‑1 (MQ=255)
gCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTtttattt > 4:517166/1‑149 (MQ=255)
cTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCgg < 2:741271/82‑1 (MQ=25)
cTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCgg > 1:741271/1‑82 (MQ=21)
gcaTCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTtttattta < 1:257625/146‑1 (MQ=255)
gTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTgcggcg < 1:832778/148‑1 (MQ=255)
atcgatcAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGc < 3:524090/105‑1 (MQ=255)
atcgatcAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGc > 4:524090/1‑105 (MQ=255)
gatcAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATg < 1:524667/148‑1 (MQ=255)
tcAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTg > 3:454855/1‑149 (MQ=255)
aTTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATTCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCAt > 3:77144/1‑149 (MQ=255)
aTTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCAt > 4:713228/1‑149 (MQ=255)
aTTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTgcgc > 4:55345/1‑149 (MQ=255)
aTTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTgcg > 1:464837/1‑148 (MQ=255)
ttACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGcgcg < 1:870377/148‑1 (MQ=255)
ttACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGcgcg > 1:817286/1‑148 (MQ=255)
cacaATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAta > 3:647764/1‑148 (MQ=255)
cacaATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAta < 4:647764/148‑1 (MQ=255)
acaATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtac > 2:663524/1‑148 (MQ=255)
aaTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtacta < 2:817286/148‑1 (MQ=255)
aTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtactac > 2:492506/1‑148 (MQ=255)
tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTGACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGt > 1:267391/1‑148 (MQ=255)
ccGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCAc > 2:878450/1‑148 (MQ=255)
|
AAATAGCCCGATGGATTTATCATCGGGCTGAGATTTATGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCAC > NC_000913/2291148‑2291417
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
