Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A25 F36 I0 R2 1302 104.9 4551290 96.3% 4382892 111.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,443 G→C 9.3% A159A (GCG→GCC) ‡ rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,4430GC 9.3% 146.4 / 6.4 43A159A (GCG→GCC) ‡rimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (17/22);  new base C (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTC  >  NC_000913/892297‑892537
                                                                                                                                                   |                                                                                              
ggCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAggcgg                                                                                               >  4:236124/1‑148 (MQ=255)
          tgcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGt                                                                                     >  4:385770/1‑148 (MQ=255)
           gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTg                                                                                    <  1:910520/148‑1 (MQ=255)
           gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTg                                                                                    <  4:121011/148‑1 (MQ=255)
           gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTg                                                                                    <  3:837685/148‑1 (MQ=255)
               gTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTg                                                                                <  3:385770/148‑1 (MQ=255)
                tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGa                                                                               <  3:236124/148‑1 (MQ=255)
                tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGa                                                                               <  1:756932/148‑1 (MQ=255)
                              cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                         >  2:855218/1‑140 (MQ=255)
                              cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                         <  1:855218/140‑1 (MQ=255)
                              cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGACCGCGGAAAGCGtca                                                                                   >  2:537192/1‑128 (MQ=255)
                              cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGACCGCGGAAAGCGtca                                                                                   <  1:537192/130‑3 (MQ=255)
                                                aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGa                                                                               >  2:281725/1‑116 (MQ=255)
                                                aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGa                                                                               <  1:281725/116‑1 (MQ=255)
                                                 gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGaa                                                                                          >  3:259593/1‑104 (MQ=255)
                                                 gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGaa                                                                                          <  4:259593/104‑1 (MQ=255)
                                                 gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGAc                                                                              >  2:937270/1‑116 (MQ=255)
                                                 gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGAc                                                                              <  1:937270/116‑1 (MQ=255)
                                                     tttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTTCAGACCGCGGAAAGCGtcaat                                                                                 >  2:300746/1‑106 (MQ=255)
                                                     tttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTTCAGACCGCGGAAAGCGtcaat                                                                                 <  1:300746/110‑5 (MQ=255)
                                                           cGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                    >  4:193288/1‑148 (MQ=255)
                                                             aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGAt                                                                                  <  1:1108976/100‑1 (MQ=255)
                                                             aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGAt                                                                                  >  2:1108976/1‑100 (MQ=255)
                                                              cATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTg                                                                                    <  2:314044/97‑1 (MQ=255)
                                                              cATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTg                                                                                    >  1:314044/1‑97 (MQ=255)
                                                                    cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGAt                                                                                  <  3:1014807/93‑1 (MQ=255)
                                                                    cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGAt                                                                                  >  4:1014807/1‑93 (MQ=255)
                                                                       tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGa                                                                               >  2:628157/1‑93 (MQ=255)
                                                                       tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGa                                                                               <  1:628157/93‑1 (MQ=255)
                                                                                 caggtggtCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                        <  3:897677/88‑1 (MQ=255)
                                                                                 cTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                        >  4:897677/1‑90 (MQ=255)
                                                                                        tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCg                                                                                      >  1:928069/1‑69 (MQ=255)
                                                                                        tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCg                                                                                      <  2:928069/69‑1 (MQ=255)
                                                                                        tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                               <  3:356399/124‑1 (MQ=255)
                                                                                        tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                               >  4:356399/1‑124 (MQ=255)
                                                                                         cAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGaaa                                                                                         <  1:96126/65‑1 (MQ=255)
                                                                                         cAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGaaa                                                                                         >  2:96126/1‑65 (MQ=255)
                                                                                             ttggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTc  <  3:193288/148‑1 (MQ=255)
                                                                                                 ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTGTGGTGCAGGAatat                                    <  4:844384/110‑1 (MQ=255)
                                                                                                 ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                    >  3:844384/1‑110 (MQ=255)
                                                                                                    aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                      <  3:816066/89‑1 (MQ=255)
                                                                                                    aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                      >  4:816066/1‑89 (MQ=255)
                                                                                                                             gCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaatg                                                           <  2:92923/59‑3 (MQ=255)
                                                                                                                             gCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                           >  1:92923/1‑59 (MQ=255)
                                                                                                                                                   |                                                                                              
GGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCG‑TCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTC  >  NC_000913/892297‑892537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A