Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F36 I0 R2
|
1302 |
104.9 |
4551290 |
96.3% |
4382892 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,302,827 |
G→A |
7.1% |
intergenic (+14/‑72) |
oppA → / → oppB |
oligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,302,827 | 0 | G | A | 7.1%
| 218.4
/ 5.0
| 70 | intergenic (+14/‑72) | oppA/oppB | oligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (31/34); new base A (2/3); total (33/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 |
TAAGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGA > NC_000913/1302681‑1302930
|
tAAGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTgggg > 2:926070/1‑148 (MQ=255)
ggATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAg > 4:981012/1‑148 (MQ=255)
gATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg < 3:1055682/148‑1 (MQ=255)
gATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg < 1:926070/148‑1 (MQ=255)
ttCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAg < 3:981012/148‑1 (MQ=255)
ccATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg < 3:404697/141‑1 (MQ=255)
ccATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg > 4:404697/1‑141 (MQ=255)
ttGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg < 3:141500/138‑1 (MQ=255)
ttGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg > 4:141500/1‑138 (MQ=255)
ttattaCTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGgtgt > 3:770149/1‑148 (MQ=255)
acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAg > 2:163065/1‑119 (MQ=255)
acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAg < 1:163065/119‑1 (MQ=255)
acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt < 3:774901/148‑1 (MQ=255)
acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGACAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt > 4:748536/1‑148 (MQ=255)
gTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg > 4:690542/1‑118 (MQ=255)
gTGAATGCGCGGCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg < 3:690542/118‑1 (MQ=255)
tGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAt < 3:720187/142‑1 (MQ=255)
tGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAt > 4:720187/1‑142 (MQ=255)
tGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTAt > 2:956107/1‑148 (MQ=255)
aTGCGCGTCTGGTGAAACCGTGGGATGGTGGCTATACCGCCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGc < 3:748536/148‑1 (MQ=255)
cgTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTAc < 4:770149/148‑1 (MQ=255)
cTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGa > 2:744323/1‑148 (MQ=255)
aaaCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGcc < 4:49762/111‑2 (MQ=255)
aaaCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGcc > 3:49762/1‑110 (MQ=255)
aaaCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGcc < 2:204408/111‑2 (MQ=255)
aaaCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGCCCCACgtat < 1:896138/119‑4 (MQ=38)
aaaCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGCCCCACgtat > 2:896138/1‑116 (MQ=38)
gggTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAg < 1:956107/148‑1 (MQ=255)
gtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGa < 2:684711/129‑1 (MQ=255)
gtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGa > 1:684711/1‑129 (MQ=255)
gtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTa < 1:744323/148‑1 (MQ=255)
gCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGa < 3:990692/85‑1 (MQ=255)
gCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGa > 4:990692/1‑85 (MQ=255)
aCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAgtgt > 3:90936/1‑84 (MQ=255)
aCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAgtgt < 4:90936/84‑1 (MQ=255)
aGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCa < 2:412784/69‑1 (MQ=255)
aGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCa > 1:412784/1‑69 (MQ=255)
tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAttttt < 2:1045103/97‑1 (MQ=255)
tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAttttt > 1:1045103/1‑97 (MQ=255)
tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGca > 2:7565/1‑115 (MQ=255)
tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGca < 1:7565/115‑1 (MQ=255)
gCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAg < 3:230392/106‑1 (MQ=255)
gCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAg > 4:230392/1‑106 (MQ=255)
ggATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGa < 1:151699/118‑1 (MQ=255)
ggATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGa > 2:151699/1‑118 (MQ=255)
ataataCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTa < 2:474721/121‑1 (MQ=255)
ataataCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTa > 1:474721/1‑121 (MQ=255)
ataCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATg > 1:237839/1‑126 (MQ=255)
ataCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATg < 2:237839/126‑1 (MQ=255)
aCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGCGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCAtt > 1:576504/1‑92 (MQ=255)
aCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCAtt < 2:576504/92‑1 (MQ=255)
aCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACaggagg > 2:896847/1‑100 (MQ=255)
aCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAgg < 1:896847/100‑1 (MQ=255)
tataCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAg < 3:165660/110‑1 (MQ=255)
tataCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAg > 4:165660/1‑110 (MQ=255)
tataCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAg < 2:268896/114‑1 (MQ=255)
tataCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAg > 1:268896/1‑114 (MQ=255)
tataCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTg > 2:197903/1‑148 (MQ=255)
taCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGa < 1:197903/148‑1 (MQ=255)
cGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtt < 3:374822/72‑1 (MQ=255)
cGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtt > 4:374822/1‑72 (MQ=255)
tAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTAc < 2:878222/107‑1 (MQ=255)
tAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTAc > 1:878222/1‑107 (MQ=255)
aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAttttt < 2:382891/50‑1 (MQ=255)
aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAttttt > 1:382891/1‑50 (MQ=255)
tGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAgg < 1:755879/70‑1 (MQ=255)
tGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAgg > 2:755879/1‑70 (MQ=255)
aTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTc > 2:3658/1‑96 (MQ=255)
aTACGTGGGGCAGGAGTGTCCTGCTACACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTc < 1:3658/96‑1 (MQ=255)
tACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAAttt > 3:40241/1‑91 (MQ=255)
tACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAAttt < 4:40241/91‑1 (MQ=255)
ggCAGGAGTGTCCTGCTCCACGGTGTCTGAttttt > 4:901543/1‑35 (MQ=255)
ggCAGGAGTGTCCTGCTCCACGGTGTCTGAttttt < 3:901543/35‑1 (MQ=255)
ggCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGa < 1:341308/104‑1 (MQ=255)
ggCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGa > 2:341308/1‑104 (MQ=255)
|
TAAGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGA > NC_000913/1302681‑1302930
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
