Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F36 I0 R2
|
1302 |
104.9 |
4551290 |
96.3% |
4382892 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,451,536 |
A→G |
9.1% |
intergenic (‑187/+61) |
tynA ← / ← paaZ |
tyramine oxidase, copper‑requiring/oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,451,536 | 0 | A | G | 9.1%
| 210.9
/ 9.3
| 66 | intergenic (‑187/+61) | tynA/paaZ | tyramine oxidase, copper‑requiring/oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (29/31); new base G (3/3); total (32/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 |
TCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGATTGA > NC_000913/1451414‑1451676
|
tCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATaaa > 3:608325/1‑148 (MQ=255)
tATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACGTTGTcacat > 3:1043198/1‑125 (MQ=255)
tATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACGTTGTcacat < 4:1043198/126‑2 (MQ=255)
tATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAg < 2:690878/133‑1 (MQ=255)
tATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAg > 1:690878/1‑133 (MQ=255)
gTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACGTTGTcacat < 1:765671/121‑2 (MQ=255)
aCTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCGTAATGTGACAACGTCACAAAACtt > 3:943161/1‑124 (MQ=255)
aCTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTa < 2:989410/125‑1 (MQ=255)
aCTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTa > 1:989410/1‑125 (MQ=255)
aCTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATCTACACATCATGCATAATGTGACAACGTCACAAAACtt < 4:943161/124‑1 (MQ=255)
aTGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACaaa > 1:416680/1‑116 (MQ=255)
aTGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACaaa < 2:974267/116‑1 (MQ=255)
aTGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACaaa < 2:416680/116‑1 (MQ=255)
aTGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACaaa > 1:974267/1‑116 (MQ=255)
cAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTg < 4:608325/148‑1 (MQ=255)
aaGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGaa > 1:534626/1‑121 (MQ=255)
aaGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGaa < 2:534626/121‑1 (MQ=255)
gggATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACGTTGTcaca > 4:878760/1‑106 (MQ=255)
gggATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACGTTGTcaca < 3:878760/106‑1 (MQ=255)
tGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCa < 3:631248/120‑1 (MQ=255)
tGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCa > 4:631248/1‑120 (MQ=255)
gCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACGTTGTcacatta > 3:399552/1‑96 (MQ=255)
tGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAAt < 1:320171/148‑1 (MQ=255)
tGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAAt < 3:248217/148‑1 (MQ=255)
ggTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAgg < 3:1033567/106‑1 (MQ=255)
ggTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAgg > 4:1033567/1‑106 (MQ=255)
tttGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTAtt < 1:1054356/110‑1 (MQ=255)
tttGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTAtt > 2:1054356/1‑110 (MQ=255)
aGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGaa < 4:796433/87‑1 (MQ=255)
aGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGaa > 3:796433/1‑87 (MQ=255)
cAGCTTTTCATACGGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAg > 2:450089/1‑86 (MQ=255)
cAGCTTTTCATACGGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAg < 1:450089/86‑1 (MQ=255)
cAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCa > 2:672005/1‑135 (MQ=255)
cAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCa < 1:672005/135‑1 (MQ=255)
aGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTa < 3:939825/73‑1 (MQ=255)
aGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTa > 4:939825/1‑73 (MQ=255)
aGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCAcc > 1:324319/1‑136 (MQ=255)
aGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCAcc < 2:324319/136‑1 (MQ=255)
ggATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAAt > 1:231244/1‑148 (MQ=255)
tGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTAtt < 2:7503/77‑1 (MQ=255)
tGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTAtt > 1:7503/1‑77 (MQ=255)
gCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCttc > 3:847038/1‑90 (MQ=255)
gCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCttc < 4:847038/90‑1 (MQ=255)
gAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAg > 2:873072/1‑148 (MQ=255)
tAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAg < 3:472889/45‑1 (MQ=255)
tAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAg > 4:472889/1‑45 (MQ=255)
tAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCa < 3:1098701/105‑1 (MQ=255)
tAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCa > 4:1098701/1‑105 (MQ=255)
gATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTaa > 1:165176/1‑91 (MQ=255)
gATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTaa < 3:863625/91‑1 (MQ=255)
gATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTaa > 4:863625/1‑91 (MQ=255)
gATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTaa < 2:165176/91‑1 (MQ=255)
gTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCggg < 2:231244/148‑1 (MQ=255)
cacaTCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACaaa > 1:9445/1‑93 (MQ=255)
cacaTCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACaaa < 2:9445/93‑1 (MQ=255)
cacaTCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGcc > 2:77301/1‑107 (MQ=255)
cacaTCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGcc < 1:77301/107‑1 (MQ=255)
acaTCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATc < 1:105855/95‑1 (MQ=255)
acaTCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATc > 2:105855/1‑95 (MQ=255)
catGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATg < 1:873072/148‑1 (MQ=255)
tGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTaa > 4:567450/1‑78 (MQ=255)
tGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTaa < 3:567450/78‑1 (MQ=255)
cATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGa > 4:928461/1‑148 (MQ=255)
aTGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGATTGa < 3:928461/148‑1 (MQ=255)
tgACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGa > 3:961479/1‑135 (MQ=255)
tgACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGa < 4:961479/135‑1 (MQ=255)
|
TCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGATTGA > NC_000913/1451414‑1451676
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
