Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F36 I0 R2
|
1302 |
104.9 |
4551290 |
96.3% |
4382892 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,810,066 |
C→A |
14.2% |
intergenic (+18/‑145) |
yniC → / → ydjM |
2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,810,066 | 0 | C | A | 14.2%
| 87.5
/ 5.0
| 28 | intergenic (+18/‑145) | yniC/ydjM | 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base A (2/2); total (14/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTT > NC_000913/1809953‑1810197
|
ccTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGc < 1:494700/121‑1 (MQ=255)
ccTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGc > 2:494700/1‑121 (MQ=255)
cGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcc < 3:722801/95‑1 (MQ=255)
cGTTGTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTAGCGGTTAATGACCAGGGGCAGTGATCTCGCTGcc > 4:722801/1‑95 (MQ=255)
aTTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCt > 2:996190/1‑96 (MQ=255)
aTTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCt < 1:996190/96‑1 (MQ=255)
cAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGa < 2:1078808/119‑1 (MQ=255)
cAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGa > 1:1078808/1‑119 (MQ=255)
cGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa < 4:767433/130‑1 (MQ=255)
cGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa > 3:767433/1‑130 (MQ=255)
cAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGccc < 1:587834/76‑1 (MQ=255)
cAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGccc > 2:587834/1‑76 (MQ=255)
cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt > 2:133791/1‑106 (MQ=255)
cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt > 4:1134687/1‑106 (MQ=255)
cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt < 1:133791/106‑1 (MQ=255)
cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt < 3:1134687/106‑1 (MQ=255)
cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACAcc < 1:968013/133‑1 (MQ=255)
cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACAcc > 2:968013/1‑133 (MQ=255)
cAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCt < 2:1030248/61‑1 (MQ=25)
cAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCt > 1:1030248/1‑61 (MQ=25)
aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTg < 2:64785/52‑1 (MQ=255)
aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTg > 1:64785/1‑52 (MQ=255)
aCCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCtt < 3:125246/50‑1 (MQ=255)
aCCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCtt > 4:125246/1‑50 (MQ=255)
ggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCaa < 2:595928/66‑1 (MQ=255)
ggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCaa > 1:595928/1‑66 (MQ=255)
ggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAAt > 3:272599/1‑116 (MQ=255)
ggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAAt < 4:272599/116‑1 (MQ=255)
tctcGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt > 3:960408/1‑133 (MQ=255)
tctcGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 4:960408/133‑1 (MQ=255)
|
CCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTT > NC_000913/1809953‑1810197
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
