Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F36 I0 R2
|
1302 |
104.9 |
4551290 |
96.3% |
4382892 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,028,158 |
G→C |
20.6% |
intergenic (‑141/+30) |
yedQ ← / ← yodC |
putative membrane‑anchored diguanylate cyclase/uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,028,158 | 0 | G | C | 20.6%
| 71.8
/ 13.2
| 30 | intergenic (‑141/+30) | yedQ/yodC | putative membrane‑anchored diguanylate cyclase/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (12/11); new base C (3/3); total (15/15) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATAC > NC_000913/2028028‑2028275
|
cccAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATTTGATCCgg < 1:967470/139‑1 (MQ=255)
cccAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg > 2:967470/1‑139 (MQ=255)
cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt > 4:656765/1‑133 (MQ=255)
cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt < 3:656765/133‑1 (MQ=255)
aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACa > 1:288773/1‑148 (MQ=255)
tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGcc < 3:570885/77‑1 (MQ=39)
tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGcc > 4:570885/1‑77 (MQ=38)
tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg > 1:989661/1‑73 (MQ=255)
tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg < 2:989661/73‑1 (MQ=255)
aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc < 4:66117/81‑1 (MQ=255)
aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc > 3:66117/1‑81 (MQ=255)
gACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTa < 2:288773/148‑1 (MQ=255)
ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGAt > 2:663062/1‑56 (MQ=255)
ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGAt > 4:433894/1‑56 (MQ=255)
ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGAt < 1:663062/56‑1 (MQ=255)
ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGAt < 3:433894/56‑1 (MQ=255)
tcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTCAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACa < 4:427432/101‑1 (MQ=255)
tcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTCAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACa > 3:427432/1‑101 (MQ=255)
attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCt < 2:457827/127‑1 (MQ=255)
attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCt > 1:457827/1‑127 (MQ=255)
ttAGCACACCAGGCCGGATGTGATCCGGCCTGATg > 2:65486/1‑35 (MQ=255)
ttAGCACACCAGGCCGGATGTGATCCGGCCTGATg < 1:65486/35‑1 (MQ=255)
tcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTc < 4:10033/91‑1 (MQ=38)
tcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTc > 3:10033/2‑92 (MQ=38)
tcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATAc > 3:463616/2‑129 (MQ=255)
tcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATAc < 4:463616/128‑1 (MQ=255)
ccAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGaa < 3:138048/60‑1 (MQ=255)
ccAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGaa > 4:138048/1‑60 (MQ=255)
ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa > 3:254062/1‑95 (MQ=255)
ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa < 4:254062/95‑1 (MQ=255)
|
CCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATAC > NC_000913/2028028‑2028275
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
