Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F36 I0 R2
|
1302 |
104.9 |
4551290 |
96.3% |
4382892 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,322,146 |
Δ1 bp |
5.9% |
coding (281/1386 nt) |
atoC → |
fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,322,146 | 0 | C | . | 5.9%
| 244.8
/ 8.9
| 68 | coding (281/1386 nt) | atoC | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base C (33/31); new base . (2/2); total (35/33) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 |
TGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCA > NC_000913/2322017‑2322263
|
tggtgTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑Ctt > 2:485326/1‑148 (MQ=255)
tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGc > 4:882075/1‑131 (MQ=255)
tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGc < 3:882075/131‑1 (MQ=255)
tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGc < 3:282964/139‑1 (MQ=255)
tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGc > 4:282964/1‑139 (MQ=255)
gCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg > 3:71872/1‑132 (MQ=255)
gCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg < 4:71872/132‑1 (MQ=255)
agagATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGAt < 1:485326/148‑1 (MQ=255)
gagaTGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt < 1:122067/142‑1 (MQ=255)
gagaTGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt > 2:122067/1‑142 (MQ=255)
aCGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa > 1:422085/1‑145 (MQ=255)
aCGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa < 2:422085/145‑1 (MQ=255)
aTCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAg < 1:181876/144‑1 (MQ=255)
aTCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAg > 2:181876/1‑144 (MQ=255)
tCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg > 2:801969/1‑111 (MQ=255)
tCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg < 1:801969/111‑1 (MQ=255)
aCTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa > 2:739549/1‑132 (MQ=255)
aCTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa < 1:739549/132‑1 (MQ=255)
cTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGttatta > 3:828110/1‑115 (MQ=255)
cTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGttatta < 4:828110/115‑1 (MQ=255)
aaaGGAGATGCGCAGCCATGAGATCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTGCGACTATGTTATTAAACCGTGTGATGTCGATGAGTTGAAGTTAATCGtt > 3:964028/1‑148 (MQ=255)
aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg < 1:490153/91‑1 (MQ=255)
aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg > 2:490153/1‑91 (MQ=255)
aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 2:600870/142‑1 (MQ=255)
aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 1:600870/1‑142 (MQ=255)
aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAG‑GCTCCGCAGCGTAGcgctt > 3:432615/1‑93 (MQ=38)
ggAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 1:367494/141‑1 (MQ=255)
ggAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 2:367494/1‑141 (MQ=255)
agaTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAgcg < 1:701789/77‑1 (MQ=255)
agaTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAgcg > 2:701789/1‑77 (MQ=255)
aTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 1:5671/1‑137 (MQ=255)
aTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 2:5671/137‑1 (MQ=255)
gcAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTa < 2:817263/131‑1 (MQ=255)
gcAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTa > 1:817263/1‑131 (MQ=255)
agaCCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt < 3:799178/99‑1 (MQ=255)
agaCCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt > 4:799178/1‑99 (MQ=255)
cccGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 1:920666/1‑121 (MQ=255)
cccGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 2:920666/121‑1 (MQ=255)
cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGtt < 1:576440/76‑1 (MQ=255)
cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGtt > 2:576440/1‑76 (MQ=255)
cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATg < 3:779514/98‑1 (MQ=255)
cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATg > 4:779514/1‑98 (MQ=255)
cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt > 1:927249/1‑126 (MQ=255)
cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt < 2:927249/126‑1 (MQ=255)
ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa > 1:634624/1‑137 (MQ=255)
ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa < 2:660128/137‑1 (MQ=255)
ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa > 1:660128/1‑137 (MQ=255)
ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa < 2:634624/137‑1 (MQ=255)
tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTaaa < 1:591784/76‑1 (MQ=255)
tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTaaa > 2:591784/1‑76 (MQ=255)
tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGaa > 4:787575/1‑99 (MQ=255)
tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGaa < 3:787575/99‑1 (MQ=255)
gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAAt > 4:175852/1‑97 (MQ=255)
gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAAt < 3:175852/97‑1 (MQ=255)
gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAgcgcg > 1:1097583/1‑113 (MQ=255)
gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAgcgcg < 2:1097583/113‑1 (MQ=255)
tgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg > 3:794789/1‑49 (MQ=255)
tgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg < 4:794789/49‑1 (MQ=255)
aCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑Ctt < 3:225817/53‑1 (MQ=255)
aCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑Ctt > 4:225817/1‑53 (MQ=255)
ccTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCa > 2:1001795/1‑148 (MQ=255)
aaGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc < 1:445250/90‑1 (MQ=255)
aaGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc > 2:445250/1‑90 (MQ=255)
aaCCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt > 1:564908/1‑93 (MQ=255)
aaCCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt < 2:564908/93‑1 (MQ=255)
catagTCGAAG‑GCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa > 4:254321/5‑93 (MQ=21)
catagTCGAAG‑GCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa < 3:901276/89‑1 (MQ=21)
catagTCGAAG‑GCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa < 3:254321/89‑1 (MQ=21)
cGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc < 2:322546/74‑1 (MQ=255)
cGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc > 1:322546/1‑74 (MQ=255)
cgcTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAag < 3:930602/102‑1 (MQ=255)
cgcTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAag > 4:930602/1‑102 (MQ=255)
|
TGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCA > NC_000913/2322017‑2322263
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
