Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A22 F44 I0 R1 387 90.4 3244086 98.5% 3195424 139.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,800,561 T→C 5.9% L26L (TTA→TTG)  infC ← translation initiation factor IF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,800,5610TC 5.9% 221.5 / 3.6 68L26L (TTA→TTG) infCtranslation initiation factor IF‑3
Reads supporting (aligned to +/- strand):  ref base T (33/31);  new base C (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01

CATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTC  >  NC_000913/1800413‑1800704
                                                                                                                                                    |                                                                                                                                               
cATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGtt                                                                                                                                                 <  3:239715/149‑1 (MQ=255)
 aTAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTa                                                                                                                                                >  2:408285/1‑149 (MQ=255)
  tAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTaa                                                                                                                                               >  1:117864/1‑149 (MQ=255)
  tAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTaa                                                                                                                                               >  2:642378/1‑149 (MQ=255)
         aTTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACt                                                                                                                                        <  3:613043/149‑1 (MQ=255)
                   aTCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGgcgc                                                                                                                              <  1:419684/149‑1 (MQ=255)
                        ttATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAAtt                                                                                                                         >  1:462420/1‑149 (MQ=255)
                                 aaaCCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTg                                                                                                                >  2:54621/1‑149 (MQ=255)
                                    ccGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATa                                                                                                             <  2:247665/149‑1 (MQ=255)
                                    ccGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATa                                                                                                             <  4:511572/149‑1 (MQ=255)
                                    ccGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATa                                                                                                             <  2:117864/149‑1 (MQ=255)
                                        cggcTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGt                                                                                                         <  3:17602/149‑1 (MQ=255)
                                            tCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAgg                                                                                                     >  3:297806/1‑149 (MQ=255)
                                             cGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAggg                                                                                                    <  1:54621/149‑1 (MQ=255)
                                                       gCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGttt                                                                                          <  2:462420/149‑1 (MQ=255)
                                                       gCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGttt                                                                                          <  2:302006/149‑1 (MQ=255)
                                                        cTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTg                                                                                         <  2:26382/149‑1 (MQ=255)
                                                          gATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGaa                                                                                       <  2:393422/149‑1 (MQ=255)
                                                          gATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCATTTGaa                                                                                       <  2:623122/149‑1 (MQ=255)
                                                            tctcGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTg                                                                                         <  2:341751/145‑1 (MQ=255)
                                                            tctcGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTg                                                                                         >  1:341751/1‑145 (MQ=255)
                                                              tcGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCg                                                                                   >  1:701467/1‑149 (MQ=255)
                                                                gACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGcgcg                                                                                                                             >  4:153130/1‑105 (MQ=255)
                                                                gACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGcgcg                                                                                                                             <  3:153130/105‑1 (MQ=255)
                                                                gACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGtt                                                                                 >  2:589255/1‑149 (MQ=255)
                                                                    aaGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTcc                                                                             <  1:408285/149‑1 (MQ=255)
                                                                    aaGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTcc                                                                             <  3:98262/149‑1 (MQ=255)
                                                                           cTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTa                                                                      >  2:342042/1‑149 (MQ=255)
                                                                            tCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTCAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTaa                                                                     >  4:286481/1‑149 (MQ=255)
                                                                            tCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTCAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTaa                                                                     >  4:803368/1‑149 (MQ=255)
                                                                             ccGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAAt                                                                    >  2:571103/1‑149 (MQ=255)
                                                                               ggCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATAc                                                                  >  1:811261/1‑149 (MQ=255)
                                                                                     ttctGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGttt                                                                                <  2:578574/129‑1 (MQ=255)
                                                                                     ttctGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGttt                                                                                >  1:578574/1‑129 (MQ=255)
                                                                                     ttctGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTAtt                                                            >  3:359723/1‑149 (MQ=255)
                                                                                     ttctGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTAtt                                                            >  4:245318/1‑149 (MQ=255)
                                                                                          cTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTCAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATtcctcc                                                       <  3:286481/149‑1 (MQ=255)
                                                                                          cTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTCAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATtcctcc                                                       <  3:803368/149‑1 (MQ=255)
                                                                                           tttCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATtc                                                           >  4:545576/1‑144 (MQ=255)
                                                                                           tttCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCa                                                      >  3:494987/1‑149 (MQ=255)
                                                                                           tttCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCa                                                      >  2:285051/1‑149 (MQ=255)
                                                                                           tttCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGCTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATtc                                                           <  3:545576/144‑1 (MQ=255)
                                                                                                  agagCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCCTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGttt                                               >  3:77853/1‑149 (MQ=255)
                                                                                                    agCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTaa                                             <  3:324668/149‑1 (MQ=255)
                                                                                                    agCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCCTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTaa                                             <  4:77853/149‑1 (MQ=255)
                                                                                                      cTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGa                                           <  2:811261/149‑1 (MQ=255)
                                                                                                       ttCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGAc                                          <  4:359723/149‑1 (MQ=255)
                                                                                                       ttCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGAc                                          >  4:439682/1‑149 (MQ=255)
                                                                                                          tctcAGACCCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTAGTCCTCCAATTGTTTAAGACTGc                                       <  2:701467/149‑1 (MQ=255)
                                                                                                              aGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCt                                   >  4:633709/1‑149 (MQ=255)
                                                                                                                    acaATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAAt                             >  1:265079/1‑149 (MQ=255)
                                                                                                                    acaATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAAt                             >  4:693728/1‑149 (MQ=255)
                                                                                                                    acaATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAAt                             >  2:217803/1‑149 (MQ=255)
                                                                                                                       aTACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAAtctc                          <  3:633709/149‑1 (MQ=255)
                                                                                                                        tACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATctct                         <  1:217803/149‑1 (MQ=255)
                                                                                                                        tACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATctct                         >  3:797503/1‑149 (MQ=255)
                                                                                                                        tACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATctct                         <  3:693728/149‑1 (MQ=255)
                                                                                                                        tACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATctct                         <  3:245318/149‑1 (MQ=255)
                                                                                                                           cAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCttgt                      >  4:391674/1‑149 (MQ=255)
                                                                                                                              gctgctCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGc                   <  1:589255/149‑1 (MQ=255)
                                                                                                                                   tCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCtt              >  1:781524/1‑149 (MQ=255)
                                                                                                                                    cGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTtc             <  1:571103/149‑1 (MQ=255)
                                                                                                                                     gCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTtct            >  1:331224/1‑149 (MQ=255)
                                                                                                                                        ttCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGa         <  4:494987/149‑1 (MQ=255)
                                                                                                                                          ccAGCCCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATc       >  4:50936/1‑149 (MQ=255)
                                                                                                                                            aGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCAc     >  4:492412/1‑149 (MQ=255)
                                                                                                                                               ccTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGTTACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTc  >  2:187056/1‑149 (MQ=255)
                                                                                                                                               ccTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATc       >  2:661410/1‑144 (MQ=255)
                                                                                                                                               ccTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATc       <  1:661410/144‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTC  >  NC_000913/1800413‑1800704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A