Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F44 I0 R1	387	90.4	3244086	98.5%	3195424	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,009,732	C→G	7.6%	intergenic (+20/‑89)	yedF → / → yedK	putative TusA family sulfurtransferase/DUF159 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,009,732	0	C	G	7.6%	220.8 / 9.2	66	intergenic (+20/‑89)	yedF/yedK	putative TusA family sulfurtransferase/DUF159 family protein
Reads supporting (aligned to +/- strand): ref base C (35/26); new base G (2/3); total (37/29)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

CTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCT  >  NC_000913/2009587‑2009873

cTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCca                                                                                                                                            >  1:271993/1‑149 (MQ=255)

 tGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCcac                                                                                                                                           >  1:109012/1‑149 (MQ=255)

       tggtgAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCggg                                                                                                                                     <  4:441525/149‑1 (MQ=255)

         gtgAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGaa                                                                                                                                   <  1:412920/149‑1 (MQ=255)

         gtgAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGaa                                                                                                                                   <  2:109012/149‑1 (MQ=255)

                       gCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTg                                                                                                                     >  4:500883/1‑149 (MQ=255)

                       gCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTg                                                                                                                     <  2:793040/149‑1 (MQ=255)

                          gTCGAGCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCACTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTAt                                                                                                                  >  4:590093/1‑149 (MQ=255)

                              aTCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCGGGaa                                                                                                                                   >  4:140194/1‑128 (MQ=255)

                              aTCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCGGGaa                                                                                                                                   <  3:140194/128‑1 (MQ=255)

                              aTCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATcaca                                                                                                              >  4:89449/1‑149 (MQ=255)

                                 aataatATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGtt                                                                                                                            <  1:92532/132‑1 (MQ=255)

                                 aataatATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGtt                                                                                                                            >  2:92532/1‑132 (MQ=255)

                                 aataatATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGAGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTTCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAg                                                                                                           >  4:589929/1‑149 (MQ=255)

                                    aataTTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGAtt                                                                                                        <  3:590093/149‑1 (MQ=255)

                                        ttCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTAc                                                                                                    >  3:423111/1‑149 (MQ=255)

                                           cACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCg                                                                                                 >  2:470928/1‑149 (MQ=255)

                                                 aTGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCa                                                                                           <  2:626317/149‑1 (MQ=255)

                                                     gcgTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACa                                                                                                                                 <  1:632075/107‑1 (MQ=255)

                                                     gcgTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACa                                                                                                                                 >  2:632075/1‑107 (MQ=255)

                                                     gcgTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTa                                                                                                                   <  2:815166/121‑1 (MQ=255)

                                                     gcgTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTa                                                                                                                   >  1:815166/1‑121 (MQ=255)

                                                        tAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCGGGaa                                                                                                                                   <  4:313024/102‑1 (MQ=255)

                                                        tAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCGGGaa                                                                                                                                   >  3:313024/1‑102 (MQ=255)

                                                           tCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCt                                                                                 >  1:407855/1‑149 (MQ=255)

                                                            cACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtt                                                                                <  3:89449/149‑1 (MQ=255)

                                                            cACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtt                                                                                >  2:756183/1‑149 (MQ=255)

                                                             aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGAttt                                                                                                       >  3:77261/1‑125 (MQ=255)

                                                             aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGAttt                                                                                                       <  4:77261/125‑1 (MQ=255)

                                                             aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCttt                                                                               >  4:736248/1‑149 (MQ=255)

                                                             aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCttt                                                                               <  3:500883/149‑1 (MQ=255)

                                                              cGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCGCACTCCTATTCtttt                                                                              <  4:591996/149‑1 (MQ=255)

                                                              cGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtttt                                                                              >  4:460282/1‑149 (MQ=255)

                                                                 cTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAg                                                                           <  2:271993/149‑1 (MQ=255)

                                                                   ataCGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGATGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTa                                                                         <  2:103140/149‑1 (MQ=255)

                                                                    taCGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTAt                                                                        <  4:423111/149‑1 (MQ=255)

                                                                           cTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCa                                                                 <  2:50930/149‑1 (MQ=255)

                                                                                 atTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGt                                                           >  2:192318/1‑149 (MQ=255)

                                                                                     aGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCt                                                       >  3:571058/1‑149 (MQ=255)

                                                                                       cAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAt                                                     <  3:736248/149‑1 (MQ=255)

                                                                                                ccGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACg                                            >  3:639229/1‑149 (MQ=255)

                                                                                                 cGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGc                                           >  2:432280/1‑149 (MQ=255)

                                                                                                  gACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCt                                          >  2:565582/1‑149 (MQ=255)

                                                                                                   aCCATTCGTTATTTAATTCATAAGTAATCCCTCATTTCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCtt                                         <  3:589929/149‑1 (MQ=255)

                                                                                                   aCCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCtt                                         <  1:470928/149‑1 (MQ=255)

                                                                                                    ccATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCttt                                        <  3:460282/149‑1 (MQ=255)

                                                                                                     cATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTg                                       <  1:756183/149‑1 (MQ=255)

                                                                                                          gTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCaa                                  >  4:784810/1‑149 (MQ=255)

                                                                                                              tttAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATccc                              >  1:399186/1‑149 (MQ=255)

                                                                                                                   ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCag                                                                <  1:715466/110‑1 (MQ=255)

                                                                                                                   ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCag                                                                >  2:715466/1‑110 (MQ=255)

                                                                                                                   ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                         >  1:309075/1‑149 (MQ=255)

                                                                                                                   ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                         >  1:210169/1‑149 (MQ=255)

                                                                                                                   ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCCATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                         >  2:86841/1‑149 (MQ=255)

                                                                                                                   ttCAGAAGTAATCCATCATTCCAGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCCATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                         >  4:443520/1‑149 (MQ=255)

                                                                                                                             aTCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGAtt               >  1:617477/1‑149 (MQ=255)

                                                                                                                              tCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTATCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGaa                   <  4:223959/144‑1 (MQ=255)

                                                                                                                              tCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGaa                   >  3:223959/1‑144 (MQ=255)

                                                                                                                              tCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTa              >  1:440778/1‑149 (MQ=255)

                                                                                                                                 cttaTTCCCGCTGTGACCCACAACGTGAACATCGTTACCTTTGTATCACATTGATCTTACCCGCCCCCACTGCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTCTGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCt           >  4:497788/4‑149 (MQ=255)

                                                                                                                                 cTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTa              >  4:263119/1‑146 (MQ=255)

                                                                                                                                 cTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTa              <  3:263119/146‑1 (MQ=255)

                                                                                                                                      tCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTgcgc      <  1:432280/149‑1 (MQ=255)

                                                                                                                                       cccGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCt     >  2:633904/1‑149 (MQ=255)

                                                                                                                                          gCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTtct  <  3:645520/149‑1 (MQ=255)

                                                                                                                                          gCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTtct  >  4:797437/1‑149 (MQ=255)

CTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCT  >  NC_000913/2009587‑2009873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A