Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F44 I0 R1	387	90.4	3244086	98.5%	3195424	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,009,789	T→C	5.2%	intergenic (+77/‑32)	yedF → / → yedK	putative TusA family sulfurtransferase/DUF159 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,009,789	0	T	C	5.2%	245.6 / 2.5	76	intergenic (+77/‑32)	yedF/yedK	putative TusA family sulfurtransferase/DUF159 family protein
Reads supporting (aligned to +/- strand): ref base T (40/32); new base C (2/2); total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC  >  NC_000913/2009646‑2009936

tCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCt                                                                                                                                                >  1:407855/1‑149 (MQ=255)

 cACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtt                                                                                                                                               <  3:89449/149‑1 (MQ=255)

 cACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtt                                                                                                                                               >  2:756183/1‑149 (MQ=255)

  aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCttt                                                                                                                                              >  4:736248/1‑149 (MQ=255)

  aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCttt                                                                                                                                              <  3:500883/149‑1 (MQ=255)

   cGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCGCACTCCTATTCtttt                                                                                                                                             <  4:591996/149‑1 (MQ=255)

   cGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtttt                                                                                                                                             >  4:460282/1‑149 (MQ=255)

      cTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAg                                                                                                                                          <  2:271993/149‑1 (MQ=255)

        ataCGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGATGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTa                                                                                                                                        <  2:103140/149‑1 (MQ=255)

         taCGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTAt                                                                                                                                       <  4:423111/149‑1 (MQ=255)

                cTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCa                                                                                                                                <  2:50930/149‑1 (MQ=255)

                      atTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGt                                                                                                                          >  2:192318/1‑149 (MQ=255)

                          aGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCt                                                                                                                      >  3:571058/1‑149 (MQ=255)

                            cAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAt                                                                                                                    <  3:736248/149‑1 (MQ=255)

                                     ccGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACg                                                                                                           >  3:639229/1‑149 (MQ=255)

                                      cGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGc                                                                                                          >  2:432280/1‑149 (MQ=255)

                                       gACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCt                                                                                                         >  2:565582/1‑149 (MQ=255)

                                        aCCATTCGTTATTTAATTCATAAGTAATCCCTCATTTCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCtt                                                                                                        <  3:589929/149‑1 (MQ=255)

                                        aCCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCtt                                                                                                        <  1:470928/149‑1 (MQ=255)

                                         ccATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCttt                                                                                                       <  3:460282/149‑1 (MQ=255)

                                          cATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTg                                                                                                      <  1:756183/149‑1 (MQ=255)

                                               gTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCaa                                                                                                 >  4:784810/1‑149 (MQ=255)

                                                   tttAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATccc                                                                                             >  1:399186/1‑149 (MQ=255)

                                                        ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCag                                                                                                                               <  1:715466/110‑1 (MQ=255)

                                                        ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCag                                                                                                                               >  2:715466/1‑110 (MQ=255)

                                                        ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                                                                                        >  1:210169/1‑149 (MQ=255)

                                                        ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                                                                                        >  1:309075/1‑149 (MQ=255)

                                                        ttCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCCATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                                                                                        >  2:86841/1‑149 (MQ=255)

                                                        ttCAGAAGTAATCCATCATTCCAGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCCATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAcg                                                                                        >  4:443520/1‑149 (MQ=255)

                                                                  aTCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGAtt                                                                              >  1:617477/1‑149 (MQ=255)

                                                                   tCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTATCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGaa                                                                                  <  4:223959/144‑1 (MQ=255)

                                                                   tCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGaa                                                                                  >  3:223959/1‑144 (MQ=255)

                                                                   tCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTa                                                                             >  1:440778/1‑149 (MQ=255)

                                                                      cttaTTCCCGCTGTGACCCACAACGTGAACATCGTTACCTTTGTATCACATTGATCTTACCCGCCCCCACTGCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTCTGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCt                                                                          >  4:497788/4‑149 (MQ=255)

                                                                      cTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTa                                                                             >  4:263119/1‑146 (MQ=255)

                                                                      cTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTa                                                                             <  3:263119/146‑1 (MQ=255)

                                                                           tCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTgcgc                                                                     <  1:432280/149‑1 (MQ=255)

                                                                            cccGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCt                                                                    >  2:633904/1‑149 (MQ=255)

                                                                               gCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTtct                                                                 >  4:797437/1‑149 (MQ=255)

                                                                               gCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTtct                                                                 <  3:645520/149‑1 (MQ=255)

                                                                                         acaGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGat                                                       >  1:101653/1‑149 (MQ=255)

                                                                                         acaGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTATTTTCAGTATGATCTCGGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGACGATTACCTTGCGCTTCTCGCGGAAGat                                                       >  4:49712/1‑149 (MQ=255)

                                                                                            gCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCCATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATAtt                                                    <  1:86841/149‑1 (MQ=255)

                                                                                            gCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCCATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATAtt                                                    <  3:443520/149‑1 (MQ=255)

                                                                                                    aTCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGa                                            <  2:617477/149‑1 (MQ=255)

                                                                                                             tttGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTccc                                     >  1:712186/1‑147 (MQ=255)

                                                                                                             tttGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTccc                                     <  2:712186/147‑1 (MQ=255)

                                                                                                             tttGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTa                                   <  2:210169/149‑1 (MQ=255)

                                                                                                                  aTCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGAt                               >  2:393074/1‑148 (MQ=255)

                                                                                                                  aTCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGAt                               <  1:393074/148‑1 (MQ=255)

                                                                                                                     acaTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGAt                               <  3:719872/145‑1 (MQ=255)

                                                                                                                     acaTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGAt                               >  4:719872/1‑145 (MQ=255)

                                                                                                                     acaTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCg                           >  1:521046/1‑149 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGAtctc                                                                                                                                 >  3:372878/1‑45 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGAtctc                                                                                                                                 <  4:372878/45‑1 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATAtt                                                    >  4:621351/1‑122 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATAtt                                                    <  3:621351/122‑1 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTccc                                     >  1:634173/1‑137 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTccc                                     <  2:634173/137‑1 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGaa                         >  4:735865/1‑149 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGaa                         <  3:747703/149‑1 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGaa                         >  4:747703/1‑149 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGaa                         >  4:794674/1‑149 (MQ=255)

                                                                                                                       aTAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGAGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGaa                         <  2:407855/149‑1 (MQ=255)

                                                                                                                        tAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAAc                        >  1:393730/1‑149 (MQ=255)

                                                                                                                            ttttACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCAt                    >  2:702268/1‑149 (MQ=255)

                                                                                                                              ttACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTg                  <  4:571058/149‑1 (MQ=255)

                                                                                                                                    gCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTcc                                      >  4:98402/1‑123 (MQ=255)

                                                                                                                                    gCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTcc                                      <  3:98402/123‑1 (MQ=255)

                                                                                                                                        ccACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACaa        >  1:396867/1‑149 (MQ=255)

                                                                                                                                        ccACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACaa        <  2:396867/149‑1 (MQ=255)

                                                                                                                                            tCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGaa                                                 >  2:784/1‑104 (MQ=255)

                                                                                                                                            tCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGaa                                                 <  1:784/104‑1 (MQ=255)

                                                                                                                                            tCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTc    <  2:399186/149‑1 (MQ=255)

                                                                                                                                              cTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTcgc  >  1:702621/1‑149 (MQ=255)

                                                                                                                                              cTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTcgc  >  2:337936/1‑149 (MQ=255)

TCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC  >  NC_000913/2009646‑2009936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A