Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A22 F44 I0 R1 387 90.4 3244086 98.5% 3195424 139.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,025,578 G→A 8.4% S23N (AGT→AAT) ‡ yedP → putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,5780GA 8.4% 226.1 / 9.0 71S23N (AGT→AAT) ‡yedPputative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (34/31);  new base A (3/3);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

CGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAA  >  NC_000913/2025430‑2025724
                                                                                                                                                    |                                                                                                                                                  
cGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAg                                                                                                                                                    <  3:93056/149‑1 (MQ=255)
cGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGAACTTGATGGCACCCTGCTGGACAGTCATAg                                                                                                                                                    <  3:510349/149‑1 (MQ=255)
  ggATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGtt                                                                                                                                                  <  4:574309/149‑1 (MQ=255)
  ggATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGtt                                                                                                                                                  <  4:318642/149‑1 (MQ=255)
    aTCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTAt                                                                                                                                                <  1:407863/149‑1 (MQ=255)
       gCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGAc                                                                                                                                             <  3:588017/149‑1 (MQ=255)
         tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTg                                                                                                                                           >  3:159842/1‑149 (MQ=255)
           ccGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAACTATGACTgtc                                                                                                                                         <  3:686983/149‑3 (MQ=255)
            cGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGCCAGTCATAACTATGACTgtcc                                                                                                                                        <  2:189600/149‑4 (MQ=255)
                   atcatcTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAACTATGACTgtcca                                                                                                                                       >  3:211958/1‑139 (MQ=255)
                   atcatcTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAACTATGACTgtcca                                                                                                                                       <  4:211958/143‑5 (MQ=255)
                    tcatcTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAg                                                                                                                                >  4:764020/1‑149 (MQ=255)
                         tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTAt                                                                                                                                                <  3:576097/128‑1 (MQ=255)
                         tgtGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACGACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTAt                                                                                                                                                >  4:576097/3‑128 (MQ=255)
                              tAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCt                                                                                                                      >  2:704880/1‑149 (MQ=255)
                                     tgCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGt                                                                                                               <  4:749696/149‑1 (MQ=255)
                                     tgCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGt                                                                                                               <  1:26963/149‑1 (MQ=255)
                                      gCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGtt                                                                                                              <  1:704880/149‑1 (MQ=255)
                                           tttGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAcgc                                                                                                         <  3:704281/149‑1 (MQ=255)
                                              gagaATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                      >  4:270068/1‑149 (MQ=255)
                                                 aaTCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCa                                                                                                   >  4:190489/1‑149 (MQ=255)
                                                  atcatcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCaa                                                                                                  >  3:287437/1‑149 (MQ=255)
                                                   tcatcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGAc                                                                                                                                             <  1:82877/105‑1 (MQ=255)
                                                   tcatcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGAc                                                                                                                                             >  2:82877/1‑105 (MQ=255)
                                                    catcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAAt                                                                                                <  3:389634/149‑1 (MQ=255)
                                                        cTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTc                                                                                            <  3:764020/149‑1 (MQ=255)
                                                          ggTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTccc                                                                                          >  2:203262/1‑149 (MQ=255)
                                                           gTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCg                                                                                         >  1:270819/1‑149 (MQ=255)
                                                             aaGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTc                                                                                       <  4:159842/149‑1 (MQ=255)
                                                              agagGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCa                                                                                      >  4:732664/1‑149 (MQ=255)
                                                               gaggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGcccc                                                                                                                            >  3:541924/1‑110 (MQ=255)
                                                               gaggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAt                                                                                     >  3:571035/1‑149 (MQ=255)
                                                               gaggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAt                                                                                     >  3:71112/1‑149 (MQ=255)
                                                               gaggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCAGAGTTATGACTGGCAACCGGCAGcccc                                                                                                                            <  4:541924/110‑1 (MQ=255)
                                                                    gTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTctct                                                                                <  4:200581/149‑1 (MQ=255)
                                                                       cTGCGCGACTATGTTTTCAATTCAACAACCCCTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTa                                                                             <  3:111169/149‑1 (MQ=255)
                                                                          cgcgACTATGTTTTCAACTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGAGAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTGTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCa                                                                          <  4:501218/149‑1 (MQ=255)
                                                                               cTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTaaa                                                                     >  2:519750/1‑149 (MQ=255)
                                                                               cTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTaaa                                                                     >  4:232629/1‑149 (MQ=255)
                                                                                    ttttCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATc                                                                >  3:131242/1‑149 (MQ=255)
                                                                                       tcaattcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGc                                                             <  1:228166/149‑1 (MQ=255)
                                                                                       tcaattcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGc                                                             <  2:270819/149‑1 (MQ=255)
                                                                                          attcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGa                                                          >  3:410116/1‑149 (MQ=255)
                                                                                           ttcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGaa                                                         >  4:576119/1‑149 (MQ=255)
                                                                                               acaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGc                                                     <  2:334466/149‑1 (MQ=255)
                                                                                                    cACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTAc                                                >  3:579471/1‑149 (MQ=255)
                                                                                                    cACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTAc                                                >  4:162228/1‑149 (MQ=255)
                                                                                                     actactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACt                                               >  1:412709/1‑149 (MQ=255)
                                                                                                      ctactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACtt                                              >  1:163322/1‑149 (MQ=255)
                                                                                                      ctactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACtt                                              <  3:732664/149‑1 (MQ=255)
                                                                                                      ctactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACtt                                              <  4:131242/149‑1 (MQ=255)
                                                                                                          tGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGa                                                                                                                                              >  1:161454/1‑49 (MQ=255)
                                                                                                          tGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGa                                                                                                                                              <  2:161454/49‑1 (MQ=255)
                                                                                                            gTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaaa                                        >  4:696957/1‑149 (MQ=255)
                                                                                                             tttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGc                                                     <  2:460923/135‑1 (MQ=255)
                                                                                                             tttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGc                                                     >  1:460923/1‑135 (MQ=255)
                                                                                                              ttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaaaaa                                      <  4:287437/149‑1 (MQ=255)
                                                                                                                  aGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGtt                                  <  3:232629/149‑1 (MQ=255)
                                                                                                                  aGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGtt                                  <  1:519750/149‑1 (MQ=255)
                                                                                                                      aTCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgggg                              >  4:623109/1‑149 (MQ=255)
                                                                                                                       tCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGc                             >  2:301063/1‑149 (MQ=255)
                                                                                                                                cACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGtt                    <  4:410116/149‑1 (MQ=255)
                                                                                                                                      gctgGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTa                                                 <  2:456210/114‑1 (MQ=255)
                                                                                                                                      gctgGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTa                                                 >  1:456210/1‑114 (MQ=255)
                                                                                                                                          gccAGTCATAACTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGAt          >  1:707780/3‑149 (MQ=255)
                                                                                                                                           ccAGTCATAACTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGAtt         >  1:791989/2‑149 (MQ=255)
                                                                                                                                           aCAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGAtt         <  1:203262/149‑1 (MQ=255)
                                                                                                                                               tCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAACCATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCg     >  2:582629/1‑149 (MQ=255)
                                                                                                                                               tCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCg     >  4:507149/1‑149 (MQ=255)
                                                                                                                                                 aTAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAgg                      <  1:802949/130‑1 (MQ=255)
                                                                                                                                                 aTAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAgg                      >  2:802949/1‑130 (MQ=255)
                                                                                                                                                 aTAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGaa   >  3:60688/1‑149 (MQ=255)
                                                                                                                                                  tAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGaaa  >  3:516248/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAA  >  NC_000913/2025430‑2025724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A