Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A35 F9 I0 R1	1164	192.0	7567036	97.0%	7340024	130.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,703,191	C→T	5.3%	A320V (GCT→GTT)	add →	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,703,191	0	C	T	5.3%	256.1 / 3.6	76	A320V (GCT→GTT)	add	adenosine deaminase
Reads supporting (aligned to +/- strand): ref base C (32/40); new base T (2/2); total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

GTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTC  >  NC_000913/1703047‑1703327

gTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTTAg                                                                                                                                      <  4:998178/149‑1 (MQ=255)

gTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAg                                                                                                                                      <  3:836721/149‑1 (MQ=255)

  gCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGa                                                                                                                                    >  2:1364080/1‑149 (MQ=255)

    cAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTGACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  <  4:1542611/149‑1 (MQ=255)

        aTTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATCAATGGTCTAGAAATGGCTTTCCTCAGCGCTGAGGAAAAAcg                                                                                                                              >  4:1549696/1‑149 (MQ=255)

         ttAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGa                                                                                                                                       >  4:421006/1‑139 (MQ=255)

         ttAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGa                                                                                                                                       <  3:421006/139‑1 (MQ=255)

         ttAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAAcgc                                                                                                                             >  2:538279/1‑149 (MQ=255)

             cacTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCAc                                                                                                                         <  1:1364080/149‑1 (MQ=255)

             cacTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCAc                                                                                                                         <  1:226900/149‑1 (MQ=255)

              acTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACt                                                                                                                        <  4:1090383/149‑1 (MQ=255)

                    gATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCgaga                                                                                                                  >  4:1032235/1‑149 (MQ=255)

                       cccGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaa                                                                                                               <  3:100800/149‑1 (MQ=255)

                        ccGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaaa                                                                                                              <  2:1278141/149‑1 (MQ=255)

                            cGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTcg                                                                                                          <  3:1673079/149‑1 (MQ=255)

                              tACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAATAAGTcgcc                                                                                                        >  2:1618828/1‑149 (MQ=255)

                               aCAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTcgccg                                                                                                       <  3:1549696/149‑1 (MQ=255)

                                 aGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGt                                                                                                            >  2:162258/1‑142 (MQ=255)

                                 aGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGt                                                                                                            <  1:162258/142‑1 (MQ=255)

                                  gggAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  <  4:839780/119‑1 (MQ=255)

                                  gggAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  >  3:839780/1‑119 (MQ=255)

                                   ggAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGaa                                                                                                   <  2:1767877/149‑1 (MQ=255)

                                      gTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  <  4:1317611/115‑1 (MQ=255)

                                      gTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  >  3:1317611/1‑115 (MQ=255)

                                      gTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTa                                                                                                >  3:1052325/1‑149 (MQ=255)

                                        ggATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAAc                                                                                              <  4:1052325/149‑1 (MQ=255)

                                         gATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  >  1:935954/1‑112 (MQ=255)

                                         gATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  <  2:935954/112‑1 (MQ=255)

                                         gATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACa                                                                                             >  1:19844/1‑149 (MQ=255)

                                           tatCATTCACGAATATACTGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGa                                                                                                                                       <  3:832787/105‑1 (MQ=255)

                                           tatCATTCACGAATATACTGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGa                                                                                                                                       >  4:832787/1‑105 (MQ=255)

                                            atCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACaaaa                                                                                          <  3:1032235/149‑1 (MQ=255)

                                               aTTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCt                                                                                                                                         >  3:681539/1‑99 (MQ=255)

                                               aTTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCt                                                                                                                                         <  4:681539/99‑1 (MQ=255)

                                                 tCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggat                                                                                     <  1:538279/149‑1 (MQ=255)

                                                 tCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggat                                                                                     <  2:19844/149‑1 (MQ=255)

                                                   aCGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGTCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTcgccg                                                                                                       <  4:1394260/129‑1 (MQ=255)

                                                   aCGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaaa                                                                                                              >  2:55383/1‑122 (MQ=255)

                                                   aCGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaaa                                                                                                              <  1:55383/122‑1 (MQ=255)

                                                   aCGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTcgccg                                                                                                       >  3:1394260/1‑129 (MQ=255)

                                                         ataCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCaaa                                                                             <  1:1618828/149‑1 (MQ=255)

                                                          taCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAAcgcg                                                                                                                            >  4:35914/1‑101 (MQ=255)

                                                          taCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAAcgcg                                                                                                                            <  3:35914/101‑1 (MQ=255)

                                                           aCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaaa                                                                                                              >  1:1472175/1‑114 (MQ=255)

                                                           aCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaaa                                                                                                              <  2:1472175/114‑1 (MQ=255)

                                                                 gCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  >  1:1705418/1‑88 (MQ=255)

                                                                 gCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGaaa                                                                                                                                  <  2:1705418/88‑1 (MQ=255)

                                                                    gcgcCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTaa                                                                                               <  3:387413/120‑1 (MQ=255)

                                                                    gcgcCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTaa                                                                                               >  4:387413/1‑120 (MQ=255)

                                                                         aGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggat                                                                                     >  3:429659/1‑125 (MQ=255)

                                                                         aGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggat                                                                                     <  4:429659/125‑1 (MQ=255)

                                                                              ctgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaaa                                                                                                              <  1:1363344/95‑1 (MQ=255)

                                                                              ctgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGaaaaa                                                                                                              >  2:1363344/1‑95 (MQ=255)

                                                                               tgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAg                                                                                                             <  3:1759409/95‑1 (MQ=255)

                                                                               tgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAg                                                                                                             >  4:1759409/1‑95 (MQ=255)

                                                                               tgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCaaa                                                                             <  4:1025070/127‑1 (MQ=255)

                                                                               tgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCaaa                                                                             >  3:1025070/1‑127 (MQ=255)

                                                                                        ccGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGTTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAc                                                                        <  2:1790981/123‑1 (MQ=255)

                                                                                        ccGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGTTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAc                                                                        >  1:1790981/1‑123 (MQ=255)

                                                                                        ccGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGTTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAc                                                                        >  1:974984/1‑123 (MQ=255)

                                                                                        ccGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGTTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAc                                                                        <  2:974984/123‑1 (MQ=255)

                                                                                            gAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggatg                                                                                    >  3:973375/1‑107 (MQ=255)

                                                                                            gAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggatg                                                                                    <  4:973375/107‑1 (MQ=255)

                                                                                            gAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggatg                                                                                    >  1:1143600/1‑107 (MQ=255)

                                                                                            gAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAatggatg                                                                                    <  2:1143600/107‑1 (MQ=255)

                                                                                                   tCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAg                                                                                                                                      >  3:482285/1‑50 (MQ=255)

                                                                                                   tCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAg                                                                                                                                      <  4:482285/50‑1 (MQ=255)

                                                                                                               aGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatc                                                                    >  4:51949/1‑104 (MQ=255)

                                                                                                               aGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatc                                                                    <  3:51949/104‑1 (MQ=255)

                                                                                                                        gTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGa                     >  4:1826137/1‑142 (MQ=255)

                                                                                                                        gTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGa                     <  3:1826137/142‑1 (MQ=255)

                                                                                                                                    cTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTc  >  1:499687/1‑149 (MQ=255)

                                                                                                                                     tttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCa                                                                         <  3:506942/77‑1 (MQ=255)

                                                                                                                                     tttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCa                                                                         >  4:506942/1‑77 (MQ=255)

                                                                                                                                        ccTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatcca                                                                  >  2:258426/1‑81 (MQ=255)

                                                                                                                                        ccTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatcca                                                                  <  1:258426/81‑1 (MQ=255)

                                                                                                                                               gcTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGc             <  4:314471/127‑1 (MQ=255)

                                                                                                                                               gcTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGc             >  3:314471/1‑127 (MQ=255)

GTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTC  >  NC_000913/1703047‑1703327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A