Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A35 F9 I0 R1
|
1164 |
192.0 |
7567036 |
97.0% |
7340024 |
130.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,823,338 |
G→C |
14.7% |
intergenic (+53/‑177) |
nadE → / → cho |
NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,823,338 | 0 | G | C | 14.7%
| 99.0
/ 8.9
| 34 | intergenic (+53/‑177) | nadE/cho | NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair |
| Reads supporting (aligned to +/- strand): ref base G (15/14); new base C (3/2); total (18/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
GTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACT > NC_000913/1823196‑1823485
|
gTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGt < 3:575464/149‑1 (MQ=255)
aaCAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCt > 3:876986/1‑149 (MQ=255)
tGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTAt < 4:876986/149‑1 (MQ=255)
gAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTc > 4:1717856/1‑149 (MQ=255)
tAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATAc > 4:1421820/1‑149 (MQ=255)
ccGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCa < 3:1421820/149‑1 (MQ=255)
cGCCAATTACCGTATTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAGATTCAATACATTGCAGGAGt < 3:1717856/149‑1 (MQ=255)
gatgatTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCt > 2:179798/1‑143 (MQ=255)
gatgatTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCt < 1:179798/143‑1 (MQ=255)
ggAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGt > 1:1573626/1‑70 (MQ=39)
ggAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGt < 2:1573626/70‑1 (MQ=255)
aaaaaGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGc < 1:59485/129‑1 (MQ=255)
aaaaaGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGc > 2:59485/1‑129 (MQ=255)
gTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTAt < 3:314660/91‑1 (MQ=255)
gTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTAt > 4:314660/1‑91 (MQ=255)
gTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGaa < 3:658127/119‑1 (MQ=255)
gTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGaa > 4:658127/1‑119 (MQ=255)
ataatTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAg > 2:594503/1‑108 (MQ=255)
ataatTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAg < 1:594503/108‑1 (MQ=255)
tatTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGgt < 3:1782404/135‑1 (MQ=255)
tatTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGgt > 4:1782404/1‑135 (MQ=255)
aTTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGAccc > 3:450226/1‑149 (MQ=255)
tGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAAc < 4:134787/113‑1 (MQ=255)
tGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAAc > 3:134787/1‑113 (MQ=255)
ttCACGCCGTATCCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCtt < 4:1244633/114‑1 (MQ=255)
ttCACGCCGCATCCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCGGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCg > 1:1082962/1‑149 (MQ=255)
ttCACGCCGCATCCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTATCCtt > 3:1244633/1‑114 (MQ=255)
tCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGc > 1:1391314/1‑149 (MQ=255)
aggCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAAt < 3:119759/135‑1 (MQ=255)
aCGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAAt > 4:119759/1‑137 (MQ=255)
gCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGt > 1:258835/1‑149 (MQ=255)
cGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCg < 4:1353008/66‑1 (MQ=255)
cGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCg > 3:1353008/1‑66 (MQ=255)
tGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACt < 4:450226/149‑1 (MQ=255)
|
GTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACT > NC_000913/1823196‑1823485
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
