Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A35 F9 I0 R1
|
1164 |
192.0 |
7567036 |
97.0% |
7340024 |
130.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,850,715 |
Δ1 bp |
18.2% |
intergenic (+22/‑145) |
sppA → / → ansA |
protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,850,715 | 0 | A | . | 18.2%
| 102.9
/ 17.9
| 33 | intergenic (+22/‑145) | sppA/ansA | protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
| Reads supporting (aligned to +/- strand): ref base A (13/14); new base . (3/3); total (16/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCAT > NC_000913/1850571‑1850861
|
aTGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc < 2:468284/149‑1 (MQ=255)
tACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc‑gtcg < 2:540248/149‑1 (MQ=255)
aCCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCgg < 1:895087/149‑1 (MQ=255)
ccTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGc < 1:608770/149‑1 (MQ=255)
gCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc‑gtc < 1:235270/143‑1 (MQ=255)
gCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc‑gtc > 2:235270/1‑143 (MQ=255)
tctACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGc > 4:950509/1‑125 (MQ=255)
tctACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGc < 3:950509/125‑1 (MQ=255)
gaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTc < 4:952722/136‑1 (MQ=255)
gaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTc > 3:952722/1‑136 (MQ=255)
gcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCat > 2:1642584/1‑141 (MQ=255)
gcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCat < 1:1642584/141‑1 (MQ=255)
gACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATa > 1:40723/1‑149 (MQ=255)
caAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCa > 1:966388/1‑149 (MQ=255)
caAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCa > 1:1496046/1‑149 (MQ=255)
tATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTc < 2:1496046/149‑1 (MQ=255)
tATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTc < 2:966388/149‑1 (MQ=255)
aCCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCt > 4:1028200/1‑109 (MQ=255)
aCCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCt < 3:1028200/109‑1 (MQ=255)
ttAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTc > 2:602475/1‑149 (MQ=255)
tCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTAtttt < 1:1177239/42‑1 (MQ=255)
tCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTAtttt > 2:1177239/1‑42 (MQ=255)
ttGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTcc < 3:1663551/84‑1 (MQ=255)
ttGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTcc > 4:1663551/1‑84 (MQ=255)
aGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATcc < 1:1382071/82‑1 (MQ=255)
aGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATcc > 2:1382071/1‑82 (MQ=255)
ggCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTc < 3:769092/131‑1 (MQ=255)
ggCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTc < 2:807600/131‑1 (MQ=255)
ggCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTc > 1:807600/1‑131 (MQ=255)
ggCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTc > 4:769092/1‑131 (MQ=255)
cGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc > 3:1430163/1‑57 (MQ=255)
cGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc < 4:1430163/57‑1 (MQ=255)
aCAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCAt > 2:982108/1‑149 (MQ=255)
|
ATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCAT > NC_000913/1850571‑1850861
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
