Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F39 I0 R1 217 77.2 2771496 98.2% 2721609 143.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,169,384 G→A 9.7% intergenic (+54/+28) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,3840GA 9.7% 174.5 / 7.4 62intergenic (+54/+28)bhsA/ldtCbiofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base G (25/31);  new base A (3/3);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

AAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCA  >  NC_000913/1169239‑1169530
                                                                                                                                                  |                                                                                                                                                   
aaaaaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCGGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCgtgt                                                                                                                                                  >  4:24577/1‑149 (MQ=255)
     gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                                                                             <  3:649284/149‑1 (MQ=255)
     gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                                                                             <  1:394299/149‑1 (MQ=255)
     gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                                                                             <  1:46079/149‑1 (MQ=255)
     gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                                                                             <  2:122637/149‑1 (MQ=255)
     gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                                                                             <  1:64716/149‑1 (MQ=255)
     gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACACGGCATGTCGCgg                                                                                                                                             <  3:355345/149‑1 (MQ=255)
      cGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                                                                            <  2:144271/149‑1 (MQ=255)
       ggATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCATGCTACTGCTGA‑TTTTTTCCTCGAGACATGCCGGGTCGCgggg                                                                                                                                           >  2:612876/1‑149 (MQ=255)
        gATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGa                                                                                                                                          >  4:345409/1‑149 (MQ=255)
            agaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑tttt                                                                                                                                     >  3:85393/1‑149 (MQ=255)
             gaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑ttttt                                                                                                                                    <  2:252225/149‑1 (MQ=255)
               tGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACACGGCATGTCGCGGGGaaaaaatc                                                                                                                                   <  4:581158/149‑8 (MQ=255)
                      aaaaTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTATCCgg                                                                                                                            >  2:557096/1‑149 (MQ=255)
                      aaaaTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCggg                                                                                                                           >  1:418547/1‑149 (MQ=255)
                      aaaaTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCggg                                                                                                                           >  1:646773/1‑149 (MQ=255)
                          tCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCttt                                                                                                                       <  3:181406/149‑1 (MQ=255)
                           cTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTa                                                                                                                      <  4:27207/149‑1 (MQ=255)
                             ttCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACa                                                                                                                    <  4:614163/149‑1 (MQ=255)
                               ccGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGc                                                                                                                  <  2:260336/149‑1 (MQ=255)
                                 gTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAGTTTATAGATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTTCCCCGAGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCg                                                                                                                 >  2:45625/1‑149 (MQ=255)
                                 gTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGt                                                                                                                >  3:373650/1‑149 (MQ=255)
                                 gTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGt                                                                                                                <  2:443836/149‑1 (MQ=255)
                                      aCTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGGCATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                                                                           <  2:418547/149‑1 (MQ=255)
                                      aCTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                                                                           >  1:46330/1‑149 (MQ=255)
                                       cTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                                                                          >  1:571213/1‑149 (MQ=255)
                                                 cGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                                                                                <  4:85393/149‑1 (MQ=255)
                                                 cGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                                                                                <  4:49159/149‑1 (MQ=255)
                                                  ggTCCGAATACCCTCCATGGAACAGCAGTAATTTGTAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgat                                                                                               <  4:176769/149‑1 (MQ=255)
                                                   gTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatg                                                                                              >  4:592515/1‑149 (MQ=255)
                                                             ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                                                                    >  4:357413/1‑149 (MQ=255)
                                                               tCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACACGGCATGTCGCgg                                                                                                                                             <  4:237665/91‑1 (MQ=255)
                                                               tCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACACGGCATGTCGCgg                                                                                                                                             >  3:237665/1‑91 (MQ=39)
                                                                ccATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATcc                                                                                 <  2:571213/149‑1 (MQ=255)
                                                                  aTGGAACTGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTTCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                                                                                <  3:451070/132‑1 (MQ=255)
                                                                  aTGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                                                                                >  4:451070/1‑132 (MQ=255)
                                                                     gAACAGTAATAATTTATAAATAATCTTTAACCCTCATCAATGCCTGCTTCTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑GTTTTTTATCAGCGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCgg                                                                            <  4:106757/149‑1 (MQ=255)
                                                                      aaCAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGc                                                                           >  1:34983/1‑149 (MQ=255)
                                                                           cagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATccc                                                                      <  3:592515/149‑1 (MQ=255)
                                                                            attAAATTAAAAAGAAAGATTAACCCTTATTAATTGCTGCTAATGCTGA‑TTTTTTCCCCGCGACCAGCCGTGTTGCCGGGATTTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaa                                                                      <  1:557096/147‑3 (MQ=255)
                                                                              tAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGa                                                                   >  2:599661/1‑149 (MQ=255)
                                                                                  ttATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg                                                               >  1:74074/1‑149 (MQ=255)
                                                                                       aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCCCACTTGATGACGGCGGACATCCACCGGCATCCCGGGACGGAcatc                                                          <  2:183758/149‑1 (MQ=255)
                                                                                        ataaGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc                                                         <  4:310563/149‑1 (MQ=255)
                                                                                          aaGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccat                                                       <  1:67626/149‑1 (MQ=255)
                                                                                                  aCCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGtt                                               <  3:303508/149‑1 (MQ=255)
                                                                                                    ccTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGc                                             >  1:325570/1‑149 (MQ=255)
                                                                                                    ccTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGc                                             <  2:507096/149‑1 (MQ=255)
                                                                                                     cTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGTGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGTACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCa                                            >  1:603332/1‑149 (MQ=255)
                                                                                                         ttAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCAc                                        <  3:637761/149‑1 (MQ=255)
                                                                                                         ttAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGATTGTGGGCTCACTTGATCACGGCGGACATCTACTGGCATCCCGGAACGGACATCCATCACATGTTGCATCAc                                        >  1:502058/1‑149 (MQ=255)
                                                                                                                       tgctgA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGttt                          <  2:546629/149‑1 (MQ=255)
                                                                                                                         ctgA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGCACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                        >  2:681691/1‑149 (MQ=255)
                                                                                                                         ctgA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                        >  2:668783/1‑149 (MQ=255)
                                                                                                                         ctgA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                        >  1:144625/1‑149 (MQ=255)
                                                                                                                         ctgA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTCTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACCTCCACCGGCCTCCCGGAACGCACATCCATCCCATGTTGCATCACTTCAGCGTCAGTTtgt                        >  2:338761/1‑149 (MQ=255)
                                                                                                                          tgA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGATATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgtg                       <  1:92205/149‑1 (MQ=255)
                                                                                                                            a‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgct                     >  2:427952/1‑149 (MQ=255)
                                                                                                                                     cccGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCttt             >  2:15092/1‑149 (MQ=255)
                                                                                                                                     cccGCGACACGGCATGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGTGTCAGTTTGTGCTGCATCttt             >  2:136450/1‑149 (MQ=255)
                                                                                                                                     cccGCGACACGGCATGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCttt             >  4:657692/1‑149 (MQ=255)
                                                                                                                                                ccGTGTGGCGGGGA‑TTTTTTTATCCGGGCTTTTCAGCGTTTGTGGGCTCACTTGATGTCGGCGGACATCCATCGGCATCCCGGAACGGACATCCATCACATCTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGattgca  <  1:338761/149‑1 (MQ=255)
                                                                                                                                                ccGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGattgca  <  2:144625/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGA‑TTTTTTCCCCGCGACATGCCGTGTCGCGGGGA‑TTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCA  >  NC_000913/1169239‑1169530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A