Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F195 I0 R2
|
483 |
52.1 |
1908604 |
98.1% |
1872340 |
138.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
897,973 |
Δ1 bp |
12.9% |
intergenic (+44/‑16) |
potI → / → ybjO |
putrescine ABC transporter permease/DUF2593 family inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 897,973 | 0 | T | . | 12.9%
| 98.1
/ 9.5
| 31 | intergenic (+44/‑16) | potI/ybjO | putrescine ABC transporter permease/DUF2593 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (13/14); new base . (2/2); total (15/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01 |
TCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTT > NC_000913/897829‑898118
|
tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTcgcg < 4:53059/149‑1 (MQ=255)
tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTcgcg > 4:39879/1‑149 (MQ=255)
tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTcgcg < 4:22316/149‑1 (MQ=255)
cGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAt < 1:277041/149‑1 (MQ=255)
cgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGttt < 1:176842/149‑1 (MQ=255)
gTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGAc < 4:304504/149‑1 (MQ=255)
tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAt < 2:186103/132‑1 (MQ=255)
tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAt > 1:186103/1‑132 (MQ=255)
tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACg > 1:132527/1‑149 (MQ=255)
tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACg > 4:277789/1‑149 (MQ=255)
ggTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAtttt < 2:132527/149‑1 (MQ=255)
gATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGaa < 4:400782/149‑1 (MQ=255)
cTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACAt < 4:249989/149‑1 (MQ=255)
tCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATc > 4:257672/1‑149 (MQ=255)
aaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGaaaa > 2:421264/1‑134 (MQ=255)
aaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGaaaa < 1:421264/134‑1 (MQ=255)
gATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCt > 2:9030/1‑149 (MQ=255)
aGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAg < 1:9030/149‑1 (MQ=255)
cTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcggc > 3:356223/1‑149 (MQ=255)
cTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcggc < 3:39879/149‑1 (MQ=255)
tGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGGGCTGGTGCAGGTGgcggcg > 3:354500/1‑149 (MQ=255)
aGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa < 2:438146/88‑1 (MQ=255)
aGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa > 1:438146/1‑88 (MQ=255)
tAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCATGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatt > 3:299199/1‑149 (MQ=255)
aaaTTTGCCAACCTGGCTACATAATGCCCCG‑CATGTCGCGGCATTGTTTTCATGGAAGTCGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCattat < 4:408512/149‑1 (MQ=255)
aacaaTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGAc > 4:394727/4‑149 (MQ=255)
tGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGt > 4:164367/1‑101 (MQ=255)
tGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGt < 3:164367/101‑1 (MQ=255)
tGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCt > 2:170857/1‑149 (MQ=255)
cg‑cATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgatga < 3:257672/149‑1 (MQ=255)
aTGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGAttt > 3:344466/1‑149 (MQ=255)
|
TCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTT > NC_000913/897829‑898118
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
