Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I0 R1 215 141.2 6785521 95.3% 6466601 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,411,637 Δ1 bp 100% coding (453/651 nt) yfbT ← sugar phosphatas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,6340C.63.7% 147.2 / 263.7 146coding (456/651 nt)yfbTsugar phosphatas
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base . (42/51);  total (71/75)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

TGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCC  >  NC_000913/2411535‑2411728
                                                                                                   |                                                                                              
tGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGc                                                                                                >  1:3150721/1‑100 (MQ=255)
tGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGc                                                                                                >  2:732871/1‑100 (MQ=255)
tGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGc                                                                                                >  1:1058619/1‑100 (MQ=255)
    ggAGCGTTACCCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCca                                                                                            <  1:134485/100‑1 (MQ=255)
      aGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGcccagca                                                                                          <  1:468202/100‑5 (MQ=255)
        cGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCcagcag                                                                                        >  2:556597/1‑100 (MQ=255)
         gTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCagcagc                                                                                       >  2:928523/1‑100 (MQ=255)
         gTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCt                                                                                      >  2:17468/1‑100 (MQ=255)
             aCCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTgcgc                                                                                  <  2:861247/100‑1 (MQ=255)
             aCCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTgcgc                                                                                  >  1:1282249/1‑100 (MQ=255)
              ccGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTgcgc                                                                                  >  2:3233911/1‑100 (MQ=255)
              ccGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTgcgc                                                                                  >  1:1634654/1‑100 (MQ=255)
              ccGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTgcgcg                                                                                 <  1:2981759/100‑1 (MQ=255)
              ccGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTgcgcg                                                                                 >  2:624686/1‑100 (MQ=255)
                gCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGcc                                                                               <  2:2557298/100‑1 (MQ=255)
                gCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGcc                                                                               <  2:1142292/100‑1 (MQ=255)
                 cAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGcc                                                                               >  2:772634/1‑100 (MQ=255)
                  aaTGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCt                                                                              <  2:3021907/100‑1 (MQ=255)
                  aaTGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTa                                                                             <  1:1335541/100‑1 (MQ=255)
                     gacatgacaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAAc                                                                           <  2:739637/100‑1 (MQ=255)
                     gacatgacaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACCCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACa                                                                          >  1:2696480/1‑100 (MQ=255)
                      acatgacaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACa                                                                          <  1:2433369/100‑1 (MQ=255)
                      acatgacaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACa                                                                          <  2:298110/100‑1 (MQ=255)
                       catgacaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGa                                                                        >  1:2253576/1‑100 (MQ=255)
                         tgacaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATa                                                                      <  1:140655/100‑1 (MQ=255)
                          gacaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATAc                                                                     >  1:442453/1‑100 (MQ=255)
                          gacaACCCGCCACCAGGCCAGAAAGCCCGCCAGCGGGAGCATCTTCCACCACCACACACCCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATAc                                                                     >  1:2887399/1‑100 (MQ=255)
                           acaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACg                                                                    <  2:3350348/100‑1 (MQ=255)
                           acaACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACg                                                                    >  2:1975183/1‑100 (MQ=255)
                             aaCCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGc                                                                   <  2:1634661/100‑1 (MQ=255)
                              aCCCGCCGCTAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCAt                                                                 >  1:2449811/1‑100 (MQ=255)
                                ccgccgCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATc                                                                >  1:2532567/1‑100 (MQ=255)
                                ccgccgCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCa                                                               <  1:81340/100‑1 (MQ=255)
                                 cgccgcCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAg                                                              <  1:388476/100‑1 (MQ=255)
                                     gccaggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTc                                                          >  2:1257743/1‑100 (MQ=255)
                                      ccaggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTc                                                          >  2:3116290/1‑100 (MQ=255)
                                      ccaggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCt                                                         >  2:2446799/1‑100 (MQ=255)
                                       caggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCt                                                         <  2:130197/100‑1 (MQ=255)
                                       caggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTg                                                        >  1:721852/1‑100 (MQ=255)
                                        aggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTgg                                                       <  2:2362864/100‑1 (MQ=255)
                                        aggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTgg                                                       <  2:2364533/100‑1 (MQ=255)
                                         ggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGt                                                      >  2:2194944/1‑100 (MQ=255)
                                         ggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGt                                                      <  2:2491742/100‑1 (MQ=255)
                                         ggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGt                                                      >  2:2194935/1‑100 (MQ=255)
                                         ggccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGt                                                      >  1:85134/1‑100 (MQ=255)
                                          gccagAAAGCACGCCAGCGGGGGCATCTACCACCACCACCCACTCCTGCGGCGCCAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTc                                                          <  1:308289/95‑1 (MQ=255)
                                          gccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGt                                                      >  2:1932174/1‑100 (MQ=255)
                                          gccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGt                                                      >  1:720048/1‑100 (MQ=255)
                                           ccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGtt                                                     >  1:282487/1‑100 (MQ=255)
                                           ccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGttt                                                    >  1:3352862/1‑100 (MQ=255)
                                           ccagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGttt                                                    >  1:177364/1‑100 (MQ=255)
                                            cagAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGttt                                                    <  1:290596/100‑1 (MQ=255)
                                             agAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTc                                                  <  2:809774/100‑1 (MQ=255)
                                              gAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTcc                                                 >  1:1141074/1‑100 (MQ=255)
                                                aaGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCcg                                                >  2:2668663/1‑100 (MQ=255)
                                                 aGCACGCCAGCGGGAGCATCTTCCACCACCACTCACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCcgcg                                              <  2:2018281/100‑1 (MQ=255)
                                                 aGCACGCCAGCGGGAGCATCTTCCACCACCACACAGTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCGAACAGATACGCATCAGGTTCTGGTTTTCcgc                                               <  1:518330/99‑1 (MQ=255)
                                                   cACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCgcgc                                             >  1:1263312/1‑100 (MQ=255)
                                                    aCGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCt                                            <  2:2314345/100‑1 (MQ=255)
                                                     cGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCtt                                           <  2:2314372/100‑1 (MQ=255)
                                                     cGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCtt                                           >  2:2115458/1‑100 (MQ=255)
                                                     cGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTc                                          <  2:3146203/100‑1 (MQ=255)
                                                      gCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATGCGCATCAGGTTCTGGTTTTCCGCGCTTc                                          <  2:84722/100‑1 (MQ=255)
                                                      gCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCa                                         >  1:1987241/1‑100 (MQ=255)
                                                       ccAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCAc                                        >  2:1744753/1‑100 (MQ=255)
                                                           cGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCg                                     >  1:336882/1‑100 (MQ=255)
                                                           cGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGc                                    <  2:447447/100‑1 (MQ=255)
                                                            gggTGCATCGTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCt                                   <  1:1726601/100‑1 (MQ=255)
                                                            gggAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGc                                    <  2:3190963/100‑1 (MQ=255)
                                                            gggAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGc                                    <  2:1110814/100‑1 (MQ=255)
                                                             ggAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCt                                   >  2:333389/1‑100 (MQ=255)
                                                             ggAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCt                                   >  2:964769/1‑100 (MQ=255)
                                                             ggAGCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTc                                  <  1:668233/100‑1 (MQ=255)
                                                                gCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGc                               >  2:438220/1‑100 (MQ=255)
                                                                gCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGc                               >  2:1836183/1‑100 (MQ=255)
                                                                gCATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGc                               <  1:257925/100‑1 (MQ=255)
                                                                 cATCTTCCACCACCACACACTCCTGCGGTGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGc                               <  2:693211/100‑1 (MQ=255)
                                                                 cATCTTCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGc                               <  2:3268924/99‑1 (MQ=255)
                                                                  aTCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCCTCAGGTTCTGGTTTTCCCCGCTTCACTATCTCAg                                >  1:204195/1‑98 (MQ=255)
                                                                   tCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCgg                             >  1:2210903/1‑100 (MQ=255)
                                                                      tCCACCACCACACCCTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTAc                         <  2:283573/100‑1 (MQ=255)
                                                                      tCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTa                          >  2:687371/1‑100 (MQ=255)
                                                                      tCCACCACCACACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTAc                         <  2:1282253/100‑1 (MQ=255)
                                                                        caccaccacACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACaa                       >  2:971974/1‑100 (MQ=255)
                                                                         accaccacACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACaa                       >  1:3200524/1‑100 (MQ=255)
                                                                          ccaccacACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAac                     >  2:2104192/1‑100 (MQ=255)
                                                                          ccaccacACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAac                     <  2:1192981/100‑1 (MQ=255)
                                                                            accacACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAacac                   >  1:581122/1‑100 (MQ=255)
                                                                             ccacACACTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACAcc                  <  2:1020417/100‑1 (MQ=255)
                                                                               acacacTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACctc                >  1:563967/1‑100 (MQ=255)
                                                                                cacacTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACctct               <  1:188746/100‑1 (MQ=255)
                                                                                 acacTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACctct               <  1:1925640/100‑1 (MQ=255)
                                                                                    cTCCTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTg           <  2:3169248/100‑1 (MQ=255)
                                                                                     tCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTg           <  1:2339956/100‑1 (MQ=255)
                                                                                       cTGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCgg        >  1:680217/1‑100 (MQ=255)
                                                                                        tGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCggg       <  2:1623209/100‑1 (MQ=255)
                                                                                        tGCGGCGCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCggg       <  1:2843205/100‑1 (MQ=255)
                                                                                           ggcgCAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAg    >  1:1754150/1‑100 (MQ=255)
                                                                                            gcgcAAG‑CCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGc   <  1:209225/100‑1 (MQ=255)
                                                                                             cgcAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTGCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGc   >  1:1474314/1‑100 (MQ=255)
                                                                                                   |                                                                                              
TGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCTCAGCGGTTACAAACACCTCTGGTGCGGGAAGCC  >  NC_000913/2411535‑2411728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A