Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I1 R1
|
99 |
64.8 |
3148104 |
96.3% |
3031624 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,867,151 |
A→G |
F134S (TTT→TCT) |
rpoS ← |
RNA polymerase, sigma S (sigma 38) factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,867,151 | 0 | A | G | 98.3%
| 211.3
/ ‑4.3
| 60 | F134S (TTT→TCT) | rpoS | RNA polymerase, sigma S (sigma 38) factor |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (29/30); total (30/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 |
TAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCA > NC_000913/2867059‑2867250
|
tAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTtctc > 1:380005/1‑100 (MQ=255)
gTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTa < 2:1378467/100‑1 (MQ=255)
tACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTAc < 1:162986/100‑1 (MQ=255)
aCGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTAcc > 2:455561/1‑100 (MQ=255)
tttGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACcgcgcg < 2:710462/100‑1 (MQ=255)
gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATc > 1:1289903/1‑100 (MQ=255)
gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATc > 2:1466988/1‑100 (MQ=255)
gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATc < 2:49802/100‑1 (MQ=255)
ttCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCa > 1:633078/1‑100 (MQ=255)
tAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGccc > 1:470492/1‑100 (MQ=255)
cGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTATCGCGCGGATCAGCCCCAgg > 2:572356/1‑100 (MQ=255)
cGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAgg > 1:1293192/1‑100 (MQ=255)
tCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCct < 1:525694/100‑1 (MQ=255)
cAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCctc > 2:452336/1‑100 (MQ=255)
aaTCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCctct > 2:586473/1‑100 (MQ=255)
tCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTc < 1:193511/100‑1 (MQ=255)
tCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTc < 2:957324/100‑1 (MQ=255)
cgccTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCg > 1:1143929/4‑100 (MQ=255)
cGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCg < 2:889548/100‑1 (MQ=255)
cGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCg > 1:64951/1‑100 (MQ=255)
cGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCg > 1:264811/1‑100 (MQ=255)
gTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGa < 1:1093377/100‑1 (MQ=255)
gTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGa < 1:576208/100‑1 (MQ=255)
tCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGAt < 1:899591/100‑1 (MQ=255)
cTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATa < 2:69210/100‑1 (MQ=255)
cTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATa < 2:884171/100‑1 (MQ=255)
cTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATa > 1:817603/1‑100 (MQ=255)
gCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAACCCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAgg < 1:1334393/100‑1 (MQ=255)
gCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAgg < 2:533178/100‑1 (MQ=255)
gCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAgg > 2:84982/1‑100 (MQ=255)
gCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAgg < 1:640486/100‑1 (MQ=255)
gAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTc > 1:1440928/1‑100 (MQ=255)
ccaccaGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGc < 1:905238/100‑1 (MQ=255)
caccaGGTTGCGTATGTTGAGAATCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCa > 1:1294415/1‑100 (MQ=255)
ccaGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAAc < 1:977860/100‑1 (MQ=255)
gTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCa > 1:44205/1‑100 (MQ=255)
tATGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCAc > 1:81024/1‑100 (MQ=255)
aTGTTGAGAAGCGGAATCCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACg < 1:886471/100‑1 (MQ=255)
aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACg > 2:623348/1‑100 (MQ=255)
aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACg < 1:663095/100‑1 (MQ=255)
tGTTGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGa > 2:1307410/1‑100 (MQ=255)
nttGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAt > 1:742044/2‑100 (MQ=255)
tGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTg > 2:117561/1‑100 (MQ=255)
tGAGAAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTg > 1:163809/1‑100 (MQ=255)
gagaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGc > 2:1205833/1‑100 (MQ=255)
agaAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGcc < 2:792125/100‑1 (MQ=255)
agaAGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGcc > 2:1452315/1‑100 (MQ=255)
aaGCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCAt < 1:432174/100‑1 (MQ=255)
gCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATaa < 2:264810/100‑1 (MQ=255)
gCGGAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATaa > 1:811561/1‑100 (MQ=255)
ggAAACCACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcg < 1:357195/100‑1 (MQ=255)
cACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGc < 2:440932/100‑1 (MQ=255)
cACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGc > 2:341229/1‑100 (MQ=255)
cACGTTCCGGGTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGc < 1:319111/100‑1 (MQ=255)
ccGGGGCAGACTTATCTACCGCGCGGATCAGCCTCAGGGTGACCTGTTCGATGAGGTCCAGCAGAGCCAGAACACGATTGCTATCACGGGCGACAAtt > 2:1193118/1‑98 (MQ=255)
ggTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTac < 2:550728/100‑1 (MQ=255)
ggTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTac > 1:33448/1‑100 (MQ=255)
gTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTacc < 1:1332544/100‑1 (MQ=255)
gTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTacc < 2:735967/100‑1 (MQ=255)
gTCAGACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTacc < 1:341230/100‑1 (MQ=255)
aaCTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTAccacca > 2:1234144/1‑100 (MQ=255)
|
TAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCA > NC_000913/2867059‑2867250
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
