Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I1 R1 102 83.9 4092546 96.1% 3932936 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,261,155 A→T D186V (GAT→GTT)  yjbM → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,261,1550AT96.6% 297.8 / 0.7 88D186V (GAT→GTT) yjbMuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (46/39);  total (48/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

ACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTT  >  NC_000913/4261059‑4261252
                                                                                                |                                                                                                 
aCCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTcc                                                                                                <  2:421806/100‑1 (MQ=255)
aCCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTcc                                                                                                >  1:771930/1‑100 (MQ=255)
  cTGGGAGAGATGAAGCGTCTGGCTGATGTGGGCGAAATGTATTTTGATTGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGGACGGTCGTTcc                                                                                                <  2:1076630/98‑1 (MQ=255)
  cTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTg                                                                                              >  1:378252/1‑100 (MQ=255)
   tGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGa                                                                                             <  2:1290994/100‑1 (MQ=255)
   tGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGa                                                                                             <  1:1866982/100‑1 (MQ=255)
   tGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGa                                                                                             <  1:1805694/100‑1 (MQ=255)
     ggAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGata                                                                                           >  1:1280874/1‑100 (MQ=255)
     ggAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGata                                                                                           >  2:1902592/1‑100 (MQ=255)
      gagagaTGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGatat                                                                                          <  2:1293200/100‑1 (MQ=255)
          gaTGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTa                                                                                      >  2:1383393/1‑100 (MQ=255)
          gaTGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTa                                                                                      <  2:78105/100‑1 (MQ=255)
            tGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTGACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAg                                                                                    >  2:1432772/1‑100 (MQ=255)
            tGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAg                                                                                    >  1:57504/1‑100 (MQ=255)
               aGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCaa                                                                                 >  1:1880343/1‑100 (MQ=255)
                gCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCaaa                                                                                >  1:321919/1‑100 (MQ=255)
                 cGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCaaaa                                                                               >  2:320720/1‑100 (MQ=255)
                  gTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAAt                                                                              >  1:29072/1‑100 (MQ=255)
                     tGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCg                                                                           <  2:965605/100‑1 (MQ=255)
                      ggCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCga                                                                          <  1:1432781/100‑1 (MQ=255)
                       gCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCgag                                                                         <  2:61619/100‑1 (MQ=255)
                       gCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCgag                                                                         >  2:908984/1‑100 (MQ=255)
                          gATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCATTCCTGATATTTTAAGCAAAATGCGAGAtg                                                                      <  2:1088896/100‑1 (MQ=255)
                           aTGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGAtgc                                                                     <  1:897361/100‑1 (MQ=255)
                            tGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGAtgct                                                                    >  2:1991461/1‑100 (MQ=255)
                              ggggAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGAtgctgc                                                                  <  1:1350198/100‑1 (MQ=255)
                              ggggAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGAtgctgc                                                                  <  1:1465426/100‑1 (MQ=255)
                               gggAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGca                                                                 <  1:524131/100‑1 (MQ=255)
                                ggAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGcac                                                                >  2:625060/1‑100 (MQ=255)
                                 gagaAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGcacc                                                               <  1:837167/100‑1 (MQ=255)
                                 gagaAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGcacc                                                               <  2:2008177/100‑1 (MQ=255)
                                  agaAATGAATTTTGATGGCTATCTGGATAATATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCCGATATTTTAAGCAAAATGCGAGATGCTGcacca                                                              <  2:1809803/100‑1 (MQ=255)
                                     aaTGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaa                                                           >  1:395746/1‑100 (MQ=255)
                                       tGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaatt                                                         >  1:1883309/1‑100 (MQ=255)
                                        gAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaatta                                                        >  1:187495/1‑100 (MQ=255)
                                        gAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaatta                                                        <  1:1161139/100‑1 (MQ=255)
                                        gAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaatta                                                        >  2:775315/1‑100 (MQ=255)
                                         aaTTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaattaa                                                       >  2:923800/1‑100 (MQ=255)
                                          aTTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaattaat                                                      >  2:23180/1‑100 (MQ=255)
                                           ttttGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaattaatt                                                     <  2:771927/100‑1 (MQ=255)
                                              tgtttgCTATCTGGATTCGCTGTCACATCTTGGGAATGAAGGATCGCTCTTTCCTGAGATTTTAAGCAAGATGCGAGATGCTGCACCACaat                                                          <  2:1937284/87‑1 (MQ=255)
                                               gATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCt                                                 <  1:320721/100‑1 (MQ=255)
                                                 tGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTc                                               <  2:1402748/100‑1 (MQ=255)
                                                     tATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGa                                           <  2:343810/100‑1 (MQ=255)
                                                      aTCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGaa                                          >  2:1222637/1‑100 (MQ=255)
                                                       tCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAg                                         <  1:1797911/100‑1 (MQ=255)
                                                        cTGGATAAGATGTCACACCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGt                                        >  2:1506346/1‑100 (MQ=255)
                                                         tGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTc                                       >  2:446965/1‑100 (MQ=255)
                                                           gATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGt                                     >  1:1983360/1‑100 (MQ=255)
                                                            aTAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGtt                                    <  2:1880325/100‑1 (MQ=255)
                                                            aTAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGtt                                    <  2:80810/100‑1 (MQ=255)
                                                             tAAGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGttt                                   <  1:1999147/100‑1 (MQ=255)
                                                              aaGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTg                                  >  2:970631/1‑100 (MQ=255)
                                                               aGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGa                                 >  1:819060/1‑100 (MQ=255)
                                                               aGATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGa                                 >  2:2035239/1‑100 (MQ=255)
                                                                gATGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGAc                                <  2:29072/100‑1 (MQ=255)
                                                                  tGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGAccc                              >  2:1597612/1‑100 (MQ=255)
                                                                  tGTCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGAccc                              >  2:710008/1‑100 (MQ=255)
                                                                    tCACATCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCaa                            >  1:312915/1‑100 (MQ=255)
                                                                     cacaTCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAAc                           >  1:1489009/1‑100 (MQ=255)
                                                                     cacaTCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAAc                           >  1:1281016/1‑100 (MQ=255)
                                                                      acaTCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAAcc                          >  1:1087112/1‑100 (MQ=255)
                                                                      acaTCTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAAcc                          >  1:1087127/1‑100 (MQ=255)
                                                                          cTGGTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCaa                      >  2:1214721/1‑100 (MQ=255)
                                                                            ggTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGATGTCGTTTGACCCAACCTCAAAg                    <  2:1185575/100‑1 (MQ=255)
                                                                             gTGAATGAAGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCAGAATTAATTAGCTTCGCGAAGTCGTTTGATCCAACCTCaaatg                   <  1:1383402/100‑3 (MQ=255)
                                                                             aaaatatcaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAgg                   >  2:1055551/9‑100 (MQ=255)
                                                                              tgaatgaaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGa                  >  2:667578/1‑100 (MQ=255)
                                                                               gaatgaaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGaa                 >  2:903246/1‑100 (MQ=255)
                                                                                aatgaaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAag                <  2:1677193/100‑1 (MQ=255)
                                                                                  tgaaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAagag              >  2:431150/1‑100 (MQ=255)
                                                                                  tgaaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAagag              <  1:1902610/100‑1 (MQ=255)
                                                                                   gaaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAgaga             >  1:572951/1‑100 (MQ=255)
                                                                                    aaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGAt            <  1:1991479/100‑1 (MQ=255)
                                                                                    aaGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGAt            >  1:1422522/1‑100 (MQ=255)
                                                                                     aGGAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGAtt           <  1:1796971/100‑1 (MQ=255)
                                                                                       gAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTaa         >  1:70816/1‑100 (MQ=255)
                                                                                       gAACGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTaa         >  2:143787/1‑100 (MQ=255)
                                                                                        aaCGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTaaa        <  1:908988/100‑1 (MQ=255)
                                                                                         aCGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTaaaa       <  1:775318/100‑1 (MQ=255)
                                                                                         aCGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTaaaa       >  2:556671/1‑100 (MQ=255)
                                                                                         aCGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTaaaa       <  1:904839/100‑1 (MQ=255)
                                                                                          cGCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAAt      <  2:1280868/100‑1 (MQ=255)
                                                                                           gCTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATa     >  1:1685807/1‑100 (MQ=255)
                                                                                            cTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATAc    >  1:1094428/1‑100 (MQ=255)
                                                                                            cTCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATAc    >  1:686287/1‑100 (MQ=255)
                                                                                             tCGTTCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACt   <  2:395745/100‑1 (MQ=255)
                                                                                              cGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACtt  >  1:1930823/1‑100 (MQ=255)
                                                                                                |                                                                                                 
ACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTT  >  NC_000913/4261059‑4261252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A