Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,922,656 T→C intergenic (‑216/+401) atpI ← / ← rsmG ATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,922,6560TC98.7% 284.9 / ‑4.3 76intergenic (‑216/+401)atpI/rsmGATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (41/34);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGTAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAAT  >  NC_000913/3922511‑3922801
                                                                                                                                                 |                                                                                                                                                 
cAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGAGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAAt                                                                                                                                               <  2:539002/150‑1 (MQ=255)
      tCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGt                                                                                                                                        >  2:440712/1‑151 (MQ=255)
       cAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGt                                                                                                                                        >  2:93056/1‑150 (MQ=255)
       cAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGt                                                                                                                                        >  1:889504/1‑150 (MQ=255)
       cAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTa                                                                                                                                       <  2:869547/151‑1 (MQ=255)
           aaGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGTAAATAACGCGTAAac                                                                                                                                    >  1:576474/1‑150 (MQ=255)
           aaGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAaca                                                                                                                                   <  2:442291/151‑1 (MQ=255)
                gTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGt                                                                                                                              <  2:432123/151‑1 (MQ=255)
                  cAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGa                                                                                                                            <  2:397996/151‑1 (MQ=255)
                   aaaTTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGAt                                                                                                                           >  1:757160/1‑151 (MQ=255)
                    aaTTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGAt                                                                                                                           >  1:521785/1‑150 (MQ=255)
                     aTTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATa                                                                                                                          >  2:536921/1‑150 (MQ=255)
                       tataCGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATaaa                                                                                                                        <  2:534569/150‑1 (MQ=255)
                              tGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCtt                                                                                                                >  1:890324/1‑151 (MQ=255)
                               gcgcCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCtt                                                                                                                <  2:90526/150‑1 (MQ=255)
                               gcgcCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTc                                                                                                               <  2:319157/151‑1 (MQ=255)
                                           tttCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATAtt                                                                                                   >  1:834978/1‑151 (MQ=255)
                                           tttCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATAtt                                                                                                   >  1:790815/1‑151 (MQ=255)
                                             tCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAACATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTgc                                                                                                 >  1:90529/1‑151 (MQ=255)
                                              cAAACAATAAGTAGGCAAAAGGTGAATAAATGGTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTgcg                                                                                                >  2:405591/1‑151 (MQ=255)
                                              cAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTgcg                                                                                                >  1:189599/1‑151 (MQ=255)
                                                aaCAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCa                                                                                              <  2:400841/151‑1 (MQ=255)
                                                  cAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATg                                                                                            <  2:104386/151‑1 (MQ=255)
                                                  cAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATg                                                                                            >  1:211082/1‑151 (MQ=255)
                                                  cAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATg                                                                                            <  2:33581/151‑1 (MQ=255)
                                                   aaTAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGt                                                                                           >  1:170472/1‑151 (MQ=255)
                                                    aTAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGtt                                                                                          >  1:895905/1‑151 (MQ=255)
                                                     tAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTc                                                                                         >  1:364090/1‑151 (MQ=255)
                                                      aaGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTATCCCTGTTCGATCATGAAAAACTGCAAATAACGCGTTAACACTGGTGATAAAGCGTGCTTCATATCACATATTGCGCATGTTcg                                                                                        <  2:184659/151‑1 (MQ=255)
                                                        gTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTcgcg                                                                                      >  1:838364/1‑151 (MQ=255)
                                                         tAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCgcgc                                                                                     >  1:126908/1‑151 (MQ=255)
                                                          aGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCgcgc                                                                                     >  1:371070/1‑150 (MQ=255)
                                                          aGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCgcgc                                                                                     >  1:775291/1‑150 (MQ=255)
                                                             cAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGcac                                                                                   >  2:868064/1‑149 (MQ=255)
                                                               aaaGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGc                                                                                >  1:279582/1‑150 (MQ=255)
                                                                aaGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGc                                                                                                                      <  1:155585/111‑1 (MQ=255)
                                                                aaGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGc                                                                                                                      >  2:155562/1‑111 (MQ=255)
                                                                     gAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCAtattt                                                                          <  2:69574/150‑1 (MQ=255)
                                                                     gAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCAtattt                                                                          >  1:646340/1‑150 (MQ=255)
                                                                       aTAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCAtatttatt                                                                       >  2:413825/1‑151 (MQ=255)
                                                                        tAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATatttattt                                                                      >  1:284608/1‑151 (MQ=255)
                                                                           aTGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACt                                                                   >  2:204267/1‑151 (MQ=255)
                                                                                 aaaTATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCa                                                              <  1:5048/150‑1 (MQ=255)
                                                                                   atatTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAat                                                           <  2:750760/151‑1 (MQ=255)
                                                                                     atTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAatg                                                          >  1:680863/1‑150 (MQ=255)
                                                                                     atTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAatg                                                          >  1:622262/1‑150 (MQ=255)
                                                                                     atTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAatg                                                          <  2:28580/150‑1 (MQ=255)
                                                                                       ttttCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAatgat                                                        <  1:347169/150‑1 (MQ=255)
                                                                                        tttCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAatgatg                                                       <  1:906466/150‑1 (MQ=255)
                                                                                          tCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCt                                                    >  1:853959/1‑151 (MQ=255)
                                                                                             aGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTg                                                 <  2:802772/151‑1 (MQ=255)
                                                                                                  aTACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGt                                            <  2:60310/151‑1 (MQ=255)
                                                                                                  aTACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGt                                            >  1:731521/1‑151 (MQ=255)
                                                                                                     cAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGttt                                          >  2:317554/1‑150 (MQ=255)
                                                                                                     cAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTa                                         <  2:171922/151‑1 (MQ=255)
                                                                                                      aaTTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTAt                                        <  2:841257/151‑1 (MQ=255)
                                                                                                      aaTTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTAt                                        >  2:778835/1‑151 (MQ=255)
                                                                                                      aaTTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTAt                                        <  1:723911/151‑1 (MQ=255)
                                                                                                          gCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGata                                    <  1:145897/151‑1 (MQ=255)
                                                                                                          gCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGat                                     <  1:545966/150‑1 (MQ=255)
                                                                                                          gCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGat                                     >  2:134168/1‑150 (MQ=255)
                                                                                                              cTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTc                                >  1:710073/1‑151 (MQ=255)
                                                                                                                  tCTGCTAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTc                            >  2:238817/1‑151 (MQ=255)
                                                                                                                       tAACCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaa                        >  1:760279/1‑150 (MQ=255)
                                                                                                                        aaCCCTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaa                       <  1:401705/150‑1 (MQ=255)
                                                                                                                          cccTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACa                    <  1:586346/151‑1 (MQ=255)
                                                                                                                           ccTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAg                   <  1:834976/151‑1 (MQ=255)
                                                                                                                           ccTGTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGCGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTGGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaaa                      >  2:590585/1‑148 (MQ=255)
                                                                                                                              gTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGa                  <  2:33410/149‑1 (MQ=255)
                                                                                                                              gTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGAt                 <  2:379727/150‑1 (MQ=255)
                                                                                                                              gTTCGATCATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATa                <  2:772889/151‑1 (MQ=255)
                                                                                                                                     cATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGtttt         >  1:721599/1‑151 (MQ=255)
                                                                                                                                     cATGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGttt          <  1:204292/150‑1 (MQ=255)
                                                                                                                                       tGAAAAACTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGCCTAAAAACAGATACTGTTTta        >  1:758594/1‑150 (MQ=255)
                                                                                                                                         aaaaaCTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTtaat      >  2:107032/1‑150 (MQ=255)
                                                                                                                                            aaCTGCAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAAt  <  2:235186/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGCATACAATTGCGACTTTTCTGCTAACCCTGTTCGATCATGAAAAACTGTAAATAACGCGTAAACACTGGTGATAAAGCGTGCTTCAGATCACATATTGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAAT  >  NC_000913/3922511‑3922801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A