Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,128,316	A→G	V27A (GTT→GCT)	metJ ←	transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,316	0	A	G	100.0%	164.2 / NA	45	V27A (GTT→GCT)	metJ	transcriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/24); total (21/24)

GCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACC  >  NC_000913/4128172‑4128460

gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTa                                                                                                                                             >  1:181606/1‑150 (MQ=255)

 catcatCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAAt                                                                                                                                           <  2:677606/151‑1 (MQ=255)

  atcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATc                                                                                                                                          <  1:598826/151‑1 (MQ=255)

        cGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTg                                                                                                                                    <  2:241950/151‑1 (MQ=255)

         ggCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGa                                                                                                                                   <  2:101752/151‑1 (MQ=255)

           cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACt                                                                                                                                 <  2:785228/151‑1 (MQ=255)

           cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACt                                                                                                                                 >  1:425975/1‑151 (MQ=255)

            aaaGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACtt                                                                                                                                >  1:352354/1‑151 (MQ=255)

            aaaGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACtt                                                                                                                                >  2:7339/1‑151 (MQ=255)

                   gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCa                                                                                                                           <  1:198280/149‑1 (MQ=255)

                        gTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACtcttct                                                                                                                    >  1:225755/1‑151 (MQ=255)

                              gCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGt                                                                                                              <  1:342616/151‑1 (MQ=255)

                              gCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGt                                                                                                              >  2:547623/1‑151 (MQ=255)

                               cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTg                                                                                                             <  2:432693/151‑1 (MQ=255)

                                     gAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGc                                                                                                       <  1:708593/151‑1 (MQ=255)

                                        aCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGt                                                                                                     >  2:726837/1‑150 (MQ=255)

                                         cGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTAt                                                                                                   >  2:803675/1‑151 (MQ=255)

                                              cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCt                                                                                              <  2:134627/151‑1 (MQ=255)

                                               gCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTg                                                                                             <  2:331581/151‑1 (MQ=255)

                                                cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGa                                                                                            <  1:150657/151‑1 (MQ=255)

                                                     cagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGatatat                                                                                       >  1:462855/1‑151 (MQ=255)

                                                        cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTcg                                                                                    >  2:228508/1‑151 (MQ=255)

                                                          cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTcgcc                                                                                  <  2:201915/151‑1 (MQ=255)

                                                          cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTcgcc                                                                                  >  1:683467/1‑151 (MQ=255)

                                                              gTTGGTAGCGTGACGCAGGTTGTTCACATGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTcgccgc                                                                                <  1:814448/149‑1 (MQ=255)

                                                                tGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTcc                                                                             >  2:505239/1‑150 (MQ=255)

                                                                 ggTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCAt                                                                           <  1:531085/151‑1 (MQ=255)

                                                                    aGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTc                                                                         >  1:696097/1‑150 (MQ=255)

                                                                         gACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCa                                                                    <  2:810939/150‑1 (MQ=255)

                                                                                gttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATAc                                                            >  1:262351/1‑151 (MQ=255)

                                                                                         cTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                     >  2:300145/1‑149 (MQ=255)

                                                                                         cTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                     <  1:300171/149‑1 (MQ=255)

                                                                                           gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCtt                                                 <  2:342242/151‑1 (MQ=255)

                                                                                             cgacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCg                                               >  1:359385/1‑151 (MQ=255)

                                                                                               acgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTc                                             >  2:115793/1‑151 (MQ=255)

                                                                                                  cgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCaa                                           <  1:447261/150‑1 (MQ=255)

                                                                                                   gtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCCGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATa                                         <  1:412017/151‑1 (MQ=255)

                                                                                                      cgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAt                                      <  1:757899/151‑1 (MQ=255)

                                                                                                       gtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                     <  2:364131/151‑1 (MQ=255)

                                                                                                            tCGGTGAGGATTTTTAACACCTTAAGAGGTATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATACTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTga                                   <  2:77298/148‑1 (MQ=255)

                                                                                                                          tAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGt                   <  2:431662/150‑1 (MQ=255)

                                                                                                                             cacCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAt               >  1:542459/1‑151 (MQ=255)

                                                                                                                                    agagGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt         >  2:548835/1‑150 (MQ=255)

                                                                                                                                          ctGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAc   >  2:223613/2‑150 (MQ=255)

                                                                                                                                           tGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAcc  >  1:904726/1‑150 (MQ=255)

GCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACC  >  NC_000913/4128172‑4128460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A