Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,319,158	T→C	Y150C (TAC→TGC)	phnJ ←	carbon‑phosphorus lyase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,319,158	0	T	C	100.0%	192.0 / NA	53	Y150C (TAC→TGC)	phnJ	carbon‑phosphorus lyase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/31); total (22/31)

GTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGTACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTC  >  NC_000913/4319016‑4319304

gTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTcc                                                                                                                                            <  2:687078/151‑1 (MQ=255)

      ttttGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGcgcg                                                                                                                                      >  2:428437/1‑151 (MQ=255)

        ttGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGt                                                                                                                                     <  1:110779/150‑1 (MQ=255)

        ttGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGt                                                                                                                                     <  1:355794/150‑1 (MQ=255)

          gggTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGc                                                                                                                                   <  1:239426/150‑1 (MQ=255)

                      ttCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGtt                                                                                                                       <  2:194337/150‑1 (MQ=255)

                      ttCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGtt                                                                                                                       <  1:74132/150‑1 (MQ=255)

                           gATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGtt                                                                                                                 >  1:707400/1‑151 (MQ=255)

                           gATCGGCGACGGGTCCATCACGTAGCGCCCATTTAACTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCAAGCGCGTGCATGGTGCGGGTTTCCGtt                                                                                                                 <  2:318736/151‑1 (MQ=255)

                                gCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTcgcg                                                                                                            >  2:720312/1‑151 (MQ=255)

                                gCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTcgcg                                                                                                            <  2:739781/151‑1 (MQ=255)

                                 cGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTcgcgc                                                                                                           >  2:894778/1‑151 (MQ=255)

                                  gACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTcgcgcg                                                                                                          >  1:904491/1‑151 (MQ=255)

                                      ggTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTc                                                                                                      <  1:775199/151‑1 (MQ=255)

                                         ccATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGa                                                                                                    <  2:825515/150‑1 (MQ=255)

                                             cACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCCTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATaaa                                                                                                <  2:683548/150‑1 (MQ=255)

                                                 tAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAgcgc                                                                                            >  1:835612/1‑150 (MQ=255)

                                                   gcgcCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGc                                                                                         <  1:797327/151‑1 (MQ=255)

                                                    cgcCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAg                                                                                          >  1:365655/1‑149 (MQ=255)

                                                         aTTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCg                                                                                   >  2:415109/1‑151 (MQ=255)

                                                                 tCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGAtt                                                                             <  1:287973/149‑1 (MQ=255)

                                                                     cGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCAcc                                                                       >  2:16947/1‑151 (MQ=255)

                                                                      ggATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCt                                                                      <  2:350512/151‑1 (MQ=255)

                                                                       gATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTg                                                                     <  2:903155/151‑1 (MQ=255)

                                                                          aGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGaa                                                                  <  2:314393/151‑1 (MQ=255)

                                                                          aGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGa                                                                   <  1:683647/150‑1 (MQ=255)

                                                                               tAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTAACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATa                                                              >  1:523204/1‑150 (MQ=255)

                                                                                    ggtggCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATc                                                        <  1:724751/151‑1 (MQ=255)

                                                                                       ggCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTg                                                      <  2:667699/150‑1 (MQ=255)

                                                                                        gCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGAt                                                    <  1:304719/151‑1 (MQ=255)

                                                                                        gCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGAt                                                    <  1:61970/151‑1 (MQ=255)

                                                                                          gATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCt                                                  >  1:890913/1‑151 (MQ=255)

                                                                                          gATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCt                                                  <  2:365629/151‑1 (MQ=255)

                                                                                          gATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCt                                                  <  1:415154/151‑1 (MQ=255)

                                                                                          gATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCt                                                  >  1:147501/1‑151 (MQ=255)

                                                                                          gATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCt                                                  <  2:43977/151‑1 (MQ=255)

                                                                                             atGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTc                                                <  1:753420/150‑1 (MQ=255)

                                                                                               gACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCgg                                              <  1:16969/150‑1 (MQ=255)

                                                                                                   gAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAgc                                         <  2:516806/151‑1 (MQ=255)

                                                                                                       cGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAgcggcg                                     >  2:121299/1‑151 (MQ=255)

                                                                                                           cGATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGttt                                  <  1:775966/150‑1 (MQ=255)

                                                                                                            gATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCg                                >  1:126721/1‑151 (MQ=255)

                                                                                                            gATATCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCg                                >  1:907061/1‑151 (MQ=255)

                                                                                                             atatCTTCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCgg                               <  1:304081/151‑1 (MQ=255)

                                                                                                                   tCATACAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGc                         >  1:178376/1‑151 (MQ=255)

                                                                                                                       aCAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTg                     >  1:406159/1‑151 (MQ=255)

                                                                                                                        cAGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTg                     >  1:34880/1‑150 (MQ=255)

                                                                                                                         aGTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGAc                   >  2:655557/1‑151 (MQ=255)

                                                                                                                          gTTTCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGAcg                  <  1:78927/151‑1 (MQ=255)

                                                                                                                             tCACCTGCATCACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGt               >  2:387066/1‑151 (MQ=255)

                                                                                                                                       cACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAAcc     >  2:906466/1‑151 (MQ=255)

                                                                                                                                       cACGCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAAc      <  2:79902/150‑1 (MQ=255)

                                                                                                                                          gCCGCACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTc  >  2:529408/1‑151 (MQ=255)

GTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCACGCCGTACTCTTCCAGCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTC  >  NC_000913/4319016‑4319304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A