Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I3 R1
|
283 |
58.3 |
1819865 |
98.8% |
1798026 |
150.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,351,153 |
G→A |
S170S (AGC→AGT) |
dcuS ← |
sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,351,153 | 0 | G | A | 100.0%
| 174.1
/ NA
| 44 | S170S (AGC→AGT) | dcuS | sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/20); total (24/20) |
GGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTC > NC_000913/4351009‑4351293
|
ggAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAAc > 2:189864/1‑151 (MQ=255)
gagaTTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACt > 2:418199/1‑151 (MQ=255)
aTTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCaa < 1:721362/151‑1 (MQ=255)
tttCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCa > 2:280756/1‑149 (MQ=255)
cGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGcc > 2:471980/1‑151 (MQ=255)
cGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGcc > 2:425456/1‑151 (MQ=255)
gTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGc < 2:21673/151‑1 (MQ=255)
ccGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGc > 1:62290/1‑151 (MQ=255)
aaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAAtt > 2:164479/1‑151 (MQ=255)
ggATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTg > 2:727473/1‑151 (MQ=255)
ttCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAt > 2:586065/1‑151 (MQ=255)
aGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAtttt < 1:686267/151‑1 (MQ=255)
gTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTc > 1:727313/1‑151 (MQ=255)
tACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTc > 1:546419/1‑150 (MQ=255)
tAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGt > 2:283174/1‑150 (MQ=255)
agacagCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATgg < 1:579666/146‑1 (MQ=255)
ccAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATAcgc < 2:89497/151‑1 (MQ=255)
aaTCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGt < 2:860672/151‑1 (MQ=255)
ccAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTgcgc < 1:730297/151‑1 (MQ=255)
cAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAAcc < 2:246884/151‑1 (MQ=255)
aaaTAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACcg > 1:355025/1‑151 (MQ=255)
aaaTAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACcg > 1:495735/1‑151 (MQ=255)
ataataCCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACcgcg < 2:481425/151‑1 (MQ=255)
aataCCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGa < 2:464259/150‑1 (MQ=255)
ataCCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGAtt < 2:827229/151‑1 (MQ=255)
aCCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGa < 2:356940/151‑1 (MQ=255)
ccGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGAt > 1:384363/1‑151 (MQ=255)
ccAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCg > 1:636434/1‑151 (MQ=255)
aGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGAc < 2:664734/151‑1 (MQ=255)
aGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGAc < 2:565795/151‑1 (MQ=255)
aaTGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACAttt > 2:353901/1‑151 (MQ=255)
aaTGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACAtt < 2:793257/150‑1 (MQ=255)
aTGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACAtttt > 2:659783/1‑151 (MQ=255)
ccAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTttcttc < 2:894682/150‑1 (MQ=255)
cAGCGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTGTGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTttctt < 2:768291/148‑1 (MQ=255)
cGACTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCAt > 2:293159/1‑151 (MQ=255)
aCTGTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTc < 1:717814/151‑1 (MQ=255)
gTCATTGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAgc < 1:586115/151‑1 (MQ=255)
tGATCTGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCttt > 2:276126/1‑151 (MQ=255)
tGTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAgg > 1:861856/1‑150 (MQ=255)
gTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAgg < 1:750636/149‑1 (MQ=255)
gTTGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGAt > 1:796453/1‑151 (MQ=255)
ttGGGTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATg > 1:228524/1‑151 (MQ=255)
gggTCACACGACTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGtc > 2:653159/1‑151 (MQ=255)
|
GGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTC > NC_000913/4351009‑4351293
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
