Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,130	G→A	R110C (CGT→TGT)	fecE ←	ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,130	0	G	A	100.0%	175.2 / NA	47	R110C (CGT→TGT)	fecE	ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/19); total (28/19)

CGGGCGTATTCTGGGCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCG  >  NC_000913/4510983‑4511275

cGGGCGTATTCTGGGCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACAccc                                                                                                                                                >  2:710068/1‑151 (MQ=255)

    cGTATTCTGGGCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACAccccc                                                                                                                                              >  2:693638/1‑149 (MQ=255)

                cAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGCCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGcc                                                                                                                                  >  2:451319/1‑149 (MQ=255)

                   gACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGa                                                                                                                             <  2:242920/151‑1 (MQ=255)

                      cATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGAtt                                                                                                                           >  1:554272/1‑150 (MQ=255)

                               aaaTGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATAc                                                                                                                 >  2:583096/1‑151 (MQ=255)

                               aaaTGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATAc                                                                                                                 >  1:577602/1‑151 (MQ=255)

                                   gcgcgcTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGaa                                                                                                              <  1:23524/150‑1 (MQ=255)

                                    cgcgcTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAAc                                                                                                            >  1:135370/1‑151 (MQ=255)

                                           gcggtgACCCCCGGAAAGCTCCGTTTACCCACCGACCGCCAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCt                                                                                                       <  2:596474/149‑1 (MQ=255)

                                               tgACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTgg                                                                                                  >  1:533680/1‑150 (MQ=255)

                                                   cgccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAGCTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACtgt                                                                                             >  2:632651/1‑151 (MQ=255)

                                                      cgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                          >  1:822880/1‑151 (MQ=255)

                                                           aGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                     <  2:197231/151‑1 (MQ=255)

                                                               cGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctct                                                                                   <  2:559255/149‑1 (MQ=255)

                                                                           gcaCGGCAAGATGATTGATCCGGGGCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTg                                                                     >  2:466224/3‑151 (MQ=255)

                                                                           gaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTg                                                                     <  2:75390/151‑1 (MQ=255)

                                                                                 cAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAgg                                                               <  1:643136/151‑1 (MQ=255)

                                                                                  aaGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgc                                                              >  1:784867/1‑151 (MQ=255)

                                                                                   aGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgca                                                             <  1:146196/151‑1 (MQ=255)

                                                                                     atgatTGATCCGGTTCCATTTCAGGGCGACATTAACTCGTGCATTGTCTTCAACGGACAGACACCCCAAGGGTGACAGCCAGGTATTAGGACCATACGAAACCAGCTCCTGAACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                            <  2:162645/150‑1 (MQ=255)

                                                                                          tgatCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCg                                                       >  2:265667/1‑150 (MQ=255)

                                                                                          tgatCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCg                                                       >  1:339976/1‑150 (MQ=255)

                                                                                            atCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                                     >  1:166619/1‑150 (MQ=255)

                                                                                                 ggTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTg                                               <  1:394512/151‑1 (MQ=255)

                                                                                                    cTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCgg                                            >  1:90876/1‑151 (MQ=255)

                                                                                                     tGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCggg                                           >  1:7455/1‑151 (MQ=255)

                                                                                                      ggTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCgg                                            <  2:64901/149‑1 (MQ=255)

                                                                                                      ggTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCg                                             <  1:560626/148‑1 (MQ=255)

                                                                                                       gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGcc                                         >  1:425430/1‑151 (MQ=255)

                                                                                                        ttCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGcc                                         >  1:192244/1‑150 (MQ=255)

                                                                                                         tCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCa                                        <  2:45053/150‑1 (MQ=255)

                                                                                                             ggCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTgg                                   >  2:181004/1‑151 (MQ=255)

                                                                                                             ggCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTg                                    <  2:710291/150‑1 (MQ=255)

                                                                                                                      aaCTCGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATg                            >  2:127615/1‑149 (MQ=255)

                                                                                                                          cGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGagag                        <  2:135805/149‑1 (MQ=255)

                                                                                                                          cGTGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGagag                        <  1:280648/149‑1 (MQ=255)

                                                                                                                            tGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCat                     >  2:681840/1‑150 (MQ=255)

                                                                                                                            tGCATTGTCTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCat                     >  1:748450/1‑150 (MQ=255)

                                                                                                                                    cTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATggg            >  2:535785/1‑151 (MQ=255)

                                                                                                                                    cTTCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATggg            >  2:760659/1‑151 (MQ=255)

                                                                                                                                      tCAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGa           <  2:7433/150‑1 (MQ=255)

                                                                                                                                       cAGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGAtt         >  1:896469/1‑151 (MQ=255)

                                                                                                                                        aGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGAtt         >  1:67633/1‑150 (MQ=255)

                                                                                                                                        aGCGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTa        <  1:812595/151‑1 (MQ=255)

                                                                                                                                          cGGAGAGACACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATc      <  2:242985/151‑1 (MQ=255)

                                                                                                                                               agaCACCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCg  >  2:468146/1‑150 (MQ=255)

CGGGCGTATTCTGGGCCAGGACCATTGCCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCG  >  NC_000913/4510983‑4511275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A