Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,785,668 C→T L137L (CTG→TTG)  gpmM → phosphoglycero mutase III, cofactor‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,785,6680CT100.0% 154.7 / NA 41L137L (CTG→TTG) gpmMphosphoglycero mutase III, cofactor‑independent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

AATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATT  >  NC_000913/3785520‑3785814
                                                                                                                                                    |                                                                                                                                                  
aaTCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTg                                                                                                                                                  >  1:407160/1‑151 (MQ=255)
        aTCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGa                                                                                                                                          <  2:656856/151‑1 (MQ=255)
          cgcgCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAAc                                                                                                                                        <  1:420602/151‑1 (MQ=255)
               tttctttGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACgcggc                                                                                                                                   >  2:315911/1‑151 (MQ=255)
                  ctttGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGgcgc                                                                                                                                 >  1:667149/1‑150 (MQ=255)
                     tGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGTCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGa                                                                                                                              <  2:199151/150‑1 (MQ=255)
                      gCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGaa                                                                                                                             <  1:76035/150‑1 (MQ=255)
                            ccGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCt                                                                                                                      <  2:167174/151‑1 (MQ=255)
                              ggTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTAc                                                                                                                    >  2:466924/1‑151 (MQ=255)
                                        ggCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCAt                                                                                                          <  2:19993/151‑1 (MQ=255)
                                         gcgcGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCAtt                                                                                                         <  1:58026/151‑1 (MQ=255)
                                                  aTAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACgg                                                                                                <  2:548719/151‑1 (MQ=255)
                                                   tAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGt                                                                                               >  1:168025/1‑151 (MQ=255)
                                                       gCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTcgcg                                                                                           >  2:446493/1‑151 (MQ=255)
                                                                  aGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTccgccg                                                                                >  2:556935/1‑151 (MQ=255)
                                                                       aaGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAg                                                                           <  1:741993/151‑1 (MQ=255)
                                                                          cGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGc                                                                        >  2:571513/1‑151 (MQ=255)
                                                                           ggTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCt                                                                       <  1:220252/151‑1 (MQ=255)
                                                                               cacaTTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAAt                                                                   <  2:725511/151‑1 (MQ=255)
                                                                                acaTTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATc                                                                  <  1:581226/151‑1 (MQ=255)
                                                                                       gggTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCCGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCt                                                            <  2:663592/150‑1 (MQ=255)
                                                                                       ggatCTGCTCCTCGACGGCGGCGTACACAGCCCGGAAGGTCACATGATGGCGATGGTAGAATGGGGAGGTGGAAGCGGCGCAGGGAAAATCTACCTGCACGCATTCCTTGCCGGTCGCGACACTCCGCCGCGCGGTGAGGGATCCt                                                                >  1:354402/4‑146 (MQ=255)
                                                                                         gTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGa                                                          >  1:158819/1‑150 (MQ=255)
                                                                                                ctcCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAAt                                                    <  2:133348/149‑1 (MQ=255)
                                                                                                    gCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAAt                                                    <  1:155374/145‑1 (MQ=255)
                                                                                                        gcggcgTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGaaaa                                          <  1:733023/151‑1 (MQ=255)
                                                                                                          ggcgTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAAt                                        >  2:479664/1‑151 (MQ=255)
                                                                                                           gcgTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAAtt                                       >  1:774374/1‑151 (MQ=255)
                                                                                                             gTACACAGCCACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTg                                     <  2:343303/151‑1 (MQ=255)
                                                                                                                      cACGAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTgg                            <  2:568508/151‑1 (MQ=255)
                                                                                                                         gAAGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCa                         <  2:63908/151‑1 (MQ=255)
                                                                                                                          aaGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCa                         >  2:386575/1‑150 (MQ=255)
                                                                                                                          aaGATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCa                         >  1:427940/1‑150 (MQ=255)
                                                                                                                            gATCACATCATGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCaa                        >  2:585759/1‑149 (MQ=255)
                                                                                                                                   tcatGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGt               >  1:753761/1‑151 (MQ=255)
                                                                                                                                     atGGCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGt               >  1:656547/1‑149 (MQ=255)
                                                                                                                                        gCGATGGTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGt          >  2:338060/1‑151 (MQ=255)
                                                                                                                                             ggTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCcat      <  2:417025/150‑1 (MQ=255)
                                                                                                                                              gTAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCcatc     >  1:670282/1‑150 (MQ=255)
                                                                                                                                               tAGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCcatcat   >  2:231196/1‑151 (MQ=255)
                                                                                                                                                aGAATTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGTCAAAGGCCGCGTAGCGTCCATCAtt  <  1:303339/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATT  >  NC_000913/3785520‑3785814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A