Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,685 A→G I219V (ATC→GTC)  wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,6850AG100.0% 175.6 / NA 50I219V (ATC→GTC) wzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/26);  total (24/26)

ACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCG  >  NC_000913/3969539‑3969830
                                                                                                                                                  |                                                                                                                                                 
aCGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTa                                                                                                                                               <  1:470783/151‑1 (MQ=255)
  gCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACg                                                                                                                                             >  1:67977/1‑151 (MQ=255)
     aataaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAcc                                                                                                                                          >  1:195682/1‑151 (MQ=255)
     aataaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAcc                                                                                                                                          <  2:704896/151‑1 (MQ=255)
       taaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAccgc                                                                                                                                        >  1:237717/1‑151 (MQ=255)
             gTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATg                                                                                                                                  >  2:481523/1‑151 (MQ=255)
                 cGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACa                                                                                                                              <  2:66122/151‑1 (MQ=255)
                  gTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAAc                                                                                                                               >  1:742807/1‑149 (MQ=255)
                      gTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCAtt                                                                                                                         <  2:766559/151‑1 (MQ=255)
                       tATGGTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCAtt                                                                                                                         <  1:761701/150‑1 (MQ=255)
                        aTGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTg                                                                                                                        >  1:512817/1‑150 (MQ=255)
                         tGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACAGCCGGATGAACAGCATTg                                                                                                                        >  1:634833/1‑149 (MQ=255)
                         tGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGGGAAGCGTCAGGAAGCGGTGGCGAAAGCCGTCTACGACCGCAGGATGAACAGCATTg                                                                                                                        >  2:492675/1‑149 (MQ=255)
                          gTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAg                                                                                                                      <  2:590300/150‑1 (MQ=255)
                          gTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAg                                                                                                                      <  1:631620/150‑1 (MQ=255)
                            tGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGAGTACCATCCAGATGAAAGCTCAGGAGAAGAGTCAGGAAGAGGTGGCGAAAGCCGTCTTCGACCGCCGGATGAACAGCATTGAg                                                                                                                      >  1:711605/1‑148 (MQ=255)
                                 ttGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCt                                                                                                              >  2:188899/1‑151 (MQ=255)
                                  tGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCt                                                                                                              <  2:298539/150‑1 (MQ=255)
                                   gccagccaGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTg                                                                                                             >  2:64889/1‑150 (MQ=255)
                                      agccagcGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGaaaa                                                                                                         <  2:541098/151‑1 (MQ=255)
                                      agccagcGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGaaa                                                                                                          >  2:679612/1‑150 (MQ=255)
                                       gccagcGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAt                                                                                                        <  1:447691/151‑1 (MQ=255)
                                                 agccagccATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGATGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagcag                                                                                              <  1:44486/151‑1 (MQ=255)
                                                  gccagccaTCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagcagc                                                                                             <  2:190876/151‑1 (MQ=255)
                                                   ccagccaTCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagcagc                                                                                             >  1:388692/1‑150 (MQ=255)
                                                    cagccaTCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCat                                                                                           <  1:64858/151‑1 (MQ=255)
                                                        caTCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataat                                                                                        >  2:743262/1‑150 (MQ=255)
                                                             gAATGATGAGCCGAAAGGCGCAGGGGTGGCGAGTAACATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGACGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAatat                                                                                      <  1:310703/147‑1 (MQ=255)
                                                                                  aTGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTTAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt                                                             >  2:515736/1‑151 (MQ=255)
                                                                                           gcgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGaa                                                    <  2:726998/151‑1 (MQ=255)
                                                                                           gcgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGa                                                     <  2:278459/150‑1 (MQ=255)
                                                                                              tACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAtt                                                  >  2:265622/1‑150 (MQ=255)
                                                                                                          gAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGaa                                     <  2:698517/151‑1 (MQ=255)
                                                                                                          gAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGa                                      <  2:430926/150‑1 (MQ=255)
                                                                                                          gAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGa                                      >  2:64067/1‑150 (MQ=255)
                                                                                                           aaaGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGaa                                     <  1:87335/150‑1 (MQ=255)
                                                                                                              gCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGt                                 <  2:770649/151‑1 (MQ=255)
                                                                                                                 cAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGtt                                <  2:383579/149‑1 (MQ=255)
                                                                                                                     tGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCt                          >  2:129073/1‑151 (MQ=255)
                                                                                                                      gAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCt                          <  2:128970/150‑1 (MQ=255)
                                                                                                                        aGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTgg                       >  2:46333/1‑151 (MQ=255)
                                                                                                                           gTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCg                    >  1:164871/1‑151 (MQ=255)
                                                                                                                             cAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTc                  <  2:103175/151‑1 (MQ=255)
                                                                                                                                    agGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCt           <  2:732947/151‑1 (MQ=255)
                                                                                                                                     ggTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTCCCTGCCGAGGAATTACCTGATTCAGAAGTGTTCCTGCTTGGGCGTCCAATGCt           <  1:286224/150‑1 (MQ=255)
                                                                                                                                         gCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAgg      <  2:122011/151‑1 (MQ=255)
                                                                                                                                           gAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCt    >  2:75809/1‑151 (MQ=255)
                                                                                                                                            aaaGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTc   >  2:600478/1‑151 (MQ=255)
                                                                                                                                             aaGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCg  >  2:561368/1‑151 (MQ=255)
                                                                                                                                             aaGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCg  >  1:2846/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCG  >  NC_000913/3969539‑3969830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A