Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,062	C→T	R344R (CGC→CGT)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,062	0	C	T	100.0%	161.3 / NA	44	R344R (CGC→CGT)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/23); total (21/23)

TGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAA  >  NC_000913/3969923‑3970207

tGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGa                                                                                                                                         >  2:528122/1‑150 (MQ=255)

 ggATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGaaa                                                                                                                                       <  1:84668/151‑1 (MQ=255)

    tCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAAt                                                                                                                                      >  1:705569/1‑149 (MQ=255)

     ccGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGc                                                                                                                                   >  1:334376/1‑151 (MQ=255)

       gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCa                                                                                                                                  >  1:538954/1‑150 (MQ=255)

            ttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACAct                                                                                                                             <  2:425621/150‑1 (MQ=255)

             tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACActg                                                                                                                            <  1:265589/150‑1 (MQ=255)

             tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGT‑GGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACActgc                                                                                                                           <  2:128838/150‑1 (MQ=255)

                  ccTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCgg                                                                                                                      >  1:763377/1‑151 (MQ=255)

                     aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTg                                                                                                                    <  1:537434/150‑1 (MQ=255)

                     aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGa                                                                                                                   <  1:711830/151‑1 (MQ=255)

                         cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAgcgc                                                                                                               >  2:137818/1‑151 (MQ=255)

                           aTTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAgcgc                                                                                                               <  2:2846/149‑1 (MQ=255)

                              tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAgg                                                                                                          >  1:575671/1‑151 (MQ=255)

                              tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCAATGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAgg                                                                                                          >  2:453688/1‑151 (MQ=255)

                               gCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGgc                                                                                                         <  2:520138/151‑1 (MQ=255)

                                   ccGCCGGAAGAACCGGAAAAACGCGATAGCCCACGACATGCCTTCCTGATGATAATGTGGGGCATTGTCGGGGGGCTGATCGGGGTTGGTGTCGAATTAACCCCTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCACAGGCGCTc                                                                                                     >  2:107710/2‑151 (MQ=255)

                                   aCGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                                                                      <  2:583305/150‑1 (MQ=255)

                                   aCGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                                                                      <  1:7853/150‑1 (MQ=255)

                                   aCGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTc                                                                                                     <  1:46657/151‑1 (MQ=255)

                                     gCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                                                                                   >  1:509705/1‑151 (MQ=255)

                                         gaagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                               >  2:468952/1‑151 (MQ=255)

                                          aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCtt                                                                                              <  1:263598/151‑1 (MQ=255)

                                            gaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                                                                            <  1:541272/151‑1 (MQ=255)

                                               ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                                                                                         <  1:743187/151‑1 (MQ=255)

                                                 ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGa                                                                                        >  2:732129/1‑150 (MQ=255)

                                                         gcgATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCg                                                                               >  1:374107/1‑151 (MQ=255)

                                                                     gtcgtGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac                                                                   >  1:133158/1‑151 (MQ=255)

                                                                       cgtGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                                 <  1:27/151‑1 (MQ=255)

                                                                        gtGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                                <  1:287976/151‑1 (MQ=255)

                                                                        gtGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                                 >  2:391898/1‑150 (MQ=255)

                                                                          gCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact                                                              <  2:711679/151‑1 (MQ=255)

                                                                                      atTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcg                                                  >  1:594505/1‑151 (MQ=255)

                                                                                              gggCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCgg                                           >  1:185947/1‑150 (MQ=255)

                                                                                                  aTTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGc                                       <  1:453842/150‑1 (MQ=255)

                                                                                                    tGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCa                                     <  1:447162/150‑1 (MQ=255)

                                                                                                         gggggCTGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCa                                  >  2:639065/1‑148 (MQ=255)

                                                                                                               tGATCGGGGCTGGTGTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGgcgc                         >  2:448226/1‑151 (MQ=255)

                                                                                                                          ggTCTCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGAAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCAt              <  1:492623/151‑1 (MQ=255)

                                                                                                                             gtCGCATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGc            <  1:468901/150‑1 (MQ=255)

                                                                                                                                 cATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTgg       <  2:512869/151‑1 (MQ=255)

                                                                                                                                 cATTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTgg       >  2:765106/1‑151 (MQ=255)

                                                                                                                                  aTTAACCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGc      >  2:65235/1‑151 (MQ=255)

                                                                                                                                      aCCCGTCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCaaaa  <  2:15411/151‑1 (MQ=255)

                                                                                                                                        ccgtcgTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCaaa   >  2:715838/1‑148 (MQ=255)

TGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAA  >  NC_000913/3969923‑3970207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A