Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,844	A→G	V112A (GTG→GCG)	rarD ←	putative chloramphenical resistance permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,844	0	A	G	100.0%	151.9 / NA	44	V112A (GTG→GCG)	rarD	putative chloramphenical resistance permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/25); total (19/25)

AAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCG  >  NC_000913/4003696‑4003985

aaaaCTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGcc                                                                                                                                             <  2:627917/151‑1 (MQ=255)

 aaaCTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCa                                                                                                                                            <  1:262202/151‑1 (MQ=255)

   aCTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGCTCGCCAGc                                                                                                                                          >  1:83232/1‑151 (MQ=255)

      aaaTGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCggg                                                                                                                                       <  1:326374/151‑1 (MQ=255)

      aaaTGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTAGCCAg                                                                                                                                           >  2:607131/1‑147 (MQ=255)

       aaTGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGt                                                                                                                                      >  2:506170/1‑151 (MQ=255)

          gCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTta                                                                                                                                    >  2:645432/1‑150 (MQ=255)

          gCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTta                                                                                                                                    >  2:536948/1‑150 (MQ=255)

           ccAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTtaat                                                                                                                                  >  2:607403/1‑151 (MQ=255)

            cAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTtaata                                                                                                                                 <  2:459400/151‑1 (MQ=255)

             aGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTtaataa                                                                                                                                <  2:456474/151‑1 (MQ=255)

                 ccAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGt                                                                                                                             >  2:283644/1‑150 (MQ=255)

                         ataataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCaa                                                                                                                       >  2:433078/1‑148 (MQ=255)

                                 tAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                                                                                                            >  1:95725/1‑151 (MQ=255)

                                  aGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                                                                                                           <  2:239601/151‑1 (MQ=255)

                                      aaCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat                                                                                                       <  1:105175/151‑1 (MQ=255)

                                         cAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCatat                                                                                                     >  1:202511/1‑150 (MQ=255)

                                         cAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATg                                                                                                    >  2:428725/1‑151 (MQ=255)

                                            aaGCCCACAGCGGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTg                                                                                                 <  1:480291/151‑1 (MQ=255)

                                                   caGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTc                                                                                          >  1:350209/1‑151 (MQ=255)

                                                    aGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCa                                                                                         <  2:732299/151‑1 (MQ=255)

                                                        ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGc                                                                                     >  2:455348/1‑151 (MQ=255)

                                                          aCTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGccc                                                                                   <  2:721772/151‑1 (MQ=255)

                                                            tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGccc                                                                                   >  1:663671/1‑149 (MQ=255)

                                                             aaCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCa                                                                                  <  2:258825/149‑1 (MQ=255)

                                                             aaCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAg                                                                                 <  1:428674/150‑1 (MQ=255)

                                                                acGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATaa                                                                             <  2:12368/151‑1 (MQ=255)

                                                                       atatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAg                                                                      <  2:11106/151‑1 (MQ=255)

                                                                             ccAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGtt                                                                >  2:34260/1‑151 (MQ=255)

                                                                                         ccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAg                                                    <  2:143176/151‑1 (MQ=255)

                                                                                          cagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                                                   >  2:117890/1‑151 (MQ=255)

                                                                                               aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAc                                                 <  1:450997/148‑1 (MQ=255)

                                                                                                 tGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCa                                             <  1:243071/150‑1 (MQ=255)

                                                                                                      ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACt                                       <  2:350243/151‑1 (MQ=255)

                                                                                                                    cgcCGAGGAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAt                         >  1:487370/1‑151 (MQ=255)

                                                                                                                          ggAAAATCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCtg                   <  1:653932/151‑1 (MQ=255)

                                                                                                                            aaaaTCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCtgtg                 <  2:619466/151‑1 (MQ=255)

                                                                                                                              aaTCATCCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGc               <  2:692999/151‑1 (MQ=255)

                                                                                                                               atcatcCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCg              <  2:706162/151‑1 (MQ=255)

                                                                                                                                 catcCCCAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGt             <  2:188994/150‑1 (MQ=255)

                                                                                                                                    ccccAGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTg          >  1:749603/1‑150 (MQ=255)

                                                                                                                                        aGCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCa     <  2:303495/151‑1 (MQ=255)

                                                                                                                                         gCACAATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGTCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAg    <  2:563550/151‑1 (MQ=255)

                                                                                                                                           acaATGTTCGCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCg  >  1:3527/1‑151 (MQ=255)

AAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCG  >  NC_000913/4003696‑4003985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A